| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,112,851 | T→A | 68.3% | V651V (GTA→GTT) | pps ← | phosphoenolpyruvate synthase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,112,851 | 0 | T | A | 68.3% | 92.7 / 59.5 | 85 | V651V (GTA→GTT) | pps | phosphoenolpyruvate synthase |
| Reads supporting (aligned to +/- strand): ref base T (19/8); new base A (16/42); total (35/50) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.11e-04 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.92e-01 | |||||||||||
CCCGTTCTCGCCACGTTTCAGCCCCTGACGCGCCAGTTCTTCAACCACCGCTTTCGCCTGATCTACGGTACGCACGAACGGGATCATGATCTCAACGTTGGTCAGTCCCATGTCGTTGCGCACACGTTT > minE/1112788‑1112916 | cccGTTCTCGCCACGTTTCAGCCCCTGACGCGCCAGTTCTTCAACCACCGCTTTCGCCTGATCTACgg < 1:3418793/68‑1 (MQ=255)cccGTTCTCACCACGTTTCAGCCCCTGATGCGCCAGTTCCTCAACCACCGCTTTCGCCTGATCAACGGt < 1:317995/69‑1 (MQ=255)cccGTTCTCACCACGTTTCAGCCCCTGATGCGCCAGTTCCTCAACCACCGCTTTCGCCTGATCAACGGt < 1:1925809/69‑1 (MQ=255)cccGTTCTCACCACGTTTCAGCCCCTGATGCGCCAGTTCCTCAACCACCGCTTTCGCCTGATCAACGGt < 1:3328127/69‑1 (MQ=255)cccGTTCTCACCACGTTTCAGCCCCTGATGCGCCAGTTCCTCAACCACCGCTTTCGCCTGATCAACGGt < 1:2288944/69‑1 (MQ=255)cccGTTCTCACCACGTTTCAGCCCCTGATGCGCCAGTTCCTCAACCACCGCTTTCGCCTGATCAACGGt < 1:544195/69‑1 (MQ=255)cccGTTCTCACCACGTTTCAGCCCCTGATGCGCCAGTTCCTCAACCACCGCTTTCGCCTGATCAACGGt < 1:2628138/69‑1 (MQ=255)cccGTTCTCACCACGTTTCAGCCCCTGATGCGCCAGTTCCTCAACCACCGCTTTCGCCTGATCAACGGt < 1:1034492/69‑1 (MQ=255)cccGTTCTCACCACGTTTCAGCCCCTGATGCGCCAGTTCCTCAACCACCGCTTTCGCCTGATCAACGGt < 1:1570227/69‑1 (MQ=255)cccGTTCTCACCACGTTTCAGCCCCTGATGCGCCAGTTCCTCAACCACCGCTTTCGCCTGATCAACGGt < 1:3227292/69‑1 (MQ=255)cccGTTCTCACCACGTTTCAGCCCCTGATGCGCCAGTTCCTCAACCACCGCTTTCGCCTGATCAACGGt < 1:2737193/69‑1 (MQ=255)cccGTTCTCACCACGTTTCAGCCCCTGATGCGCCAGTTCCTCAACCACCGCTTTCGCCTGATCAACGGt < 1:1291284/69‑1 (MQ=255)cccGTTCTCACCACGTTTCAGCCCCTGATGCGCCAGTTCCTCAACCACCGCTTTCGCCTGATCAACGGt < 1:2817975/69‑1 (MQ=255)cccGTTCTCACCACGTTTCAGCCCCTGATGCGCCAGTTCCTCAACCACCGCTTTCGCCTGATCAACGGt < 1:2820836/69‑1 (MQ=255)cccGTTCTCACCACGTTTCAGCCCCTGATGCGCCAGTTCCTCAACCACCGCTTTCGCCTGATCAACGGt < 1:2916267/69‑1 (MQ=255)cccGTTCTCACCACGTTTCAGCCCCTGATGCGCCAGTTCCTCAACCACCGCTTTCGCCTGATCAACGGt < 1:3108989/69‑1 (MQ=255)cccGTTCTCACCACGTTTCAGCCCCTGATGCGCCAGTTCCTCAACCACCGCTTTCGCCTGATCAACGGt < 1:929303/69‑1 (MQ=255)cccGTTCTCACCACGTTTCAGCCCCTGATGCGCCAGTTCCTCAACCACCGCTTTCGCCTGATCAACCGt < 1:3163005/69‑1 (MQ=255) ccGTTCTCACCACGTTTCAGCCCCTGATGCGCCAGTTCCTCAACCACCGCTTTCGCCTGATCAACGGt < 1:493064/68‑1 (MQ=255) ccGTTCTCACCACGTTTCAGCCCCTGATGCGCCAGTTCCTCAACCACCGCTTTCGCCTGATCAACGGt < 1:188708/68‑1 (MQ=255) ctcGCCACGTTTCAGCCCCTGACGCGCCAGTTCTTCAACCACCGCTTTCGCCTGATCTACGGTACGCa > 1:2785149/1‑68 (MQ=255) ctcGCCACGTTTCAGCCCCTGACGCGCCAGTTCTTCAACCACCGCTTTCGCCTGATCTACGGTACGCa > 1:3197036/1‑68 (MQ=255) ctcGCCACGTTTCAGCCCCTGACGCGCCAGTTCTTCAACCACCGCTTTCGCCTGATCTACGGTACGCa > 1:3227671/1‑68 (MQ=255) ctcGCCACGTTTCAGCCCCTGACGCGCCAGTTCTTCAACCACCGCTTTCGCCTGATCTACGGTACGCa > 1:2248657/1‑68 (MQ=255) ctcGCCACGTTTCAGCCCCTGACGCGCCAGTTCTTCAACCACCGCTTTCGCCTGATCTACGGTACGCa > 1:731676/1‑68 (MQ=255) ctcGCCACGTTTCAGCCCCTGACGCGCCAGTTCTTCAACCACCGCTTTCGCCTGATCTACGGTACGCa > 1:1453614/1‑68 (MQ=255) gTTTCAGCCCCTGATGCGCCAGTTCCTCAACCACCGCTTTCGCCTGATCAACGGTACGCACGAACggg > 1:774937/1‑68 (MQ=255) gTTTCAGCCCCTGATGCGCCAGTTCCTCAACCACCGCTTTCGCCTGATCAACGGTACGCACGAACggg > 1:340341/1‑68 (MQ=255) gTTTCAGCCCCTGATGCGCCAGTTCCTCAACCACCGCTTTCGCCTGATCAACGGTACGCACGAACggg > 1:1543061/1‑68 (MQ=255) gTTTCAGCCCCTGATGCGCCAGTTCCTCAACCACCGCTTTCGCCTGATCAACGGTACGCACGAACggg > 1:272334/1‑68 (MQ=255) gTTTCAGCCCCTGATGCGCCAGTTCCTCAACCACCGCTTTCGCCTGATCAACGGTACGCACGAACggg > 1:2728363/1‑68 (MQ=255) gTTTCAGCCCCTGATGCGCCAGTTCCTCAACCACCGCTTTCGCCTGATCAACGGTACGCACGAACggg > 1:2742419/1‑68 (MQ=255) gTTTCAGCCCCTGATGCGCCAGTTCCTCAACCACCGCTTTCGCCTGATCAACGGTACGCACGAACggg > 1:824543/1‑68 (MQ=255) gTTTCAGCCCCTGATGCGCCAGTTCCTCAACCACCGCTTTCGCCTGATCAACGGTACGCACGAACggg > 1:1233903/1‑68 (MQ=255) gTTTCAGCCCCTGATGCGCCAGTTCCTCAACCACCGCTTTCGCCTGATCAACGGTACGCACGAACggg > 1:179038/1‑68 (MQ=255) gTTTCAGCCCCTGATGCGCCAGTTCCTCAACCACCGCTTTCGCCTGATCAACGGTACGCACGAACggg > 1:3013331/1‑68 (MQ=255) gTTTCAGCCCCTGATGCGCCAGTTCCTCAACCACCGCTTTCGCCTGATCAACGGTACGCACGAACggg > 1:3049002/1‑68 (MQ=255) gTTTCAGCCCCTGATGCGCCAGTTCCTCAACCACCGCTTTCGCCTGATCAACGGTACGCACGAACggg > 1:1147270/1‑68 (MQ=255) gTTTCAGCCCCTGATGCGCCAGTTCCTCAACCACCGCTTTCGCCTGATCAACGGTACGCACGAACggg > 1:1874741/1‑68 (MQ=255) gTTTCAGCCCCTGATGCGCCAGTTCCTCAACCACCGCTTTCGCCTGATCAACGGTACGCACGAACggg > 1:340276/1‑68 (MQ=255) gTTTCAGCCCCTGATGCGCCAGTTCCTCAACCACCGCTTTCGCCTGATCAACGGTACGCACGAACggg > 1:1879779/1‑68 (MQ=255) gTTTCAGCCCCTGATACGCCAGTTCCTCAACCACCGCTTTCGCCTGATCAACGGTACGCACGAACggg > 1:1614958/1‑68 (MQ=255) tcttcAACCACCGCTTTCGCCTGATCTACGGTACGCAc > 1:2634094/1‑38 (MQ=255) tcttcAACCACCGCTTTCGCCTGATCTACGGTACGCACGAACGGGATCATGATCTCAACGTTGGTCAGt > 1:2679807/1‑69 (MQ=255) tcttcAACCACCGCTTTCGCCTGATCTACGGTACGCACGAACGGGATCATGATCTCAACGTTGGTCAGt > 1:1448764/1‑69 (MQ=255) cttcAACCACCGCTTTCGCCTGATCTACGGTACGCACGAACGGGATCATGATGTCAACGTTGGTCAGt > 1:2844170/1‑68 (MQ=255) cttcAACCACCGCTTTCGCCTGATCTACGGTACGCACGAACGGGATCATGATCTCAACGTTGGTCAg > 1:954172/1‑67 (MQ=255) cttcAACCACCGCTTTCGCCTGATCTACGGTACGCACGAACGGGATCATGATCTCAACGTTGGTCAGt > 1:2565099/1‑68 (MQ=255) cttcAACCACCGCTTTCGCCTGATCTACGGTACGCACGAACGGGATCATGATCTCAACGTTGGTCAGt > 1:3243025/1‑68 (MQ=255) cttcAACCACCGCTTTCGCCTGATCTACGGTACGCACGAACGGGATCATGATCTCAACGTTGGTCAGt > 1:3297703/1‑68 (MQ=255) cttcAACCACCGCTTTCGCCTGATCTACGGTACGCACGAACGGGATCATGATCTCAACGTTGGTCAGt > 1:1740035/1‑68 (MQ=255) cttcAACCACCGCTTTCGCCTGATCTACGGTACGCACGAACGGGATCATGATCTCAACGTTGGTCAGt > 1:2267364/1‑68 (MQ=255) cttcAACCACCGCTTTCGCCTGATCTACGGTACGCACGAACGGGATCATGATCTCAACGTTGGTCAGt > 1:2147378/1‑68 (MQ=255) cttcAACCACCGCTTTCGCCTGATCTACGGTACGCACGAACGGGATCATGATCTCAACGTTGGTCAGt > 1:3381236/1‑68 (MQ=255) tcAACCACCGCTTTCGCCTGATCTACGGTACGCACGAACGGGATCATGATCTCAACGTTGGTCAGTcc < 1:2502539/68‑1 (MQ=255) tcAACCACCGCTTTCGCCTGATCTACGGTACGCACGAACGGGATCATGATCTCAACGTTGGTCAGTcc < 1:2768308/68‑1 (MQ=255) tcAACCACCGCTTTCGCCTGATCTACGGTACGCACGAACGGGATCATGATCTCAACGTTGGTCAGTcc < 1:321662/68‑1 (MQ=255) tcAACCACCGCTTTCGCCTGATCTACGGTACGCACGAACGGGATCATGATCTCAACGTTGGTCAGTcc < 1:2838268/68‑1 (MQ=255) tcAACCACCGCTTTCGCCTGATCTACGGTACGCACGAACGGGATCATGATCTCAACGTTGGTCAGTcc < 1:123030/68‑1 (MQ=255) tcAACCACCGCTTTCGCCTGATCTACGGTACGCACGAACGGGATCATGATCTCAACGTTGGTCAGTcc < 1:197099/68‑1 (MQ=255) cAACCACCGCTTTCGCCTGATCAACGGTACGCACGAACGGGATCATGATCTCAACGTTAGTCa < 1:2647247/63‑1 (MQ=255) cAACCACCGCTTTCGCCTGATCAACGGTACGCACGAACGGGATCATGATCTCAACGTTAGTCa < 1:850119/63‑1 (MQ=255) cAACCACCGCTTTCGCCTGATCAACGGTACGCACGAACGGGATCATGATCTCAACGTTAGTCa < 1:3180785/63‑1 (MQ=255) cAACCACCGCTTTCGCCTGATCAACGGTACGCACGAACGGGATCATGATCTCAACGTTAGTCa < 1:52477/63‑1 (MQ=255) cAACCACCGCTTTCGCCTGATCAACGGTACGCACGAACGGGATCATGATCTCAACGTTAGTCa < 1:2814617/63‑1 (MQ=255) cAACCACCGCTTTCGCCTGATCAACGGTACGCACGAACGGGATCATGATCTCAACGTTAGTCa < 1:1155375/63‑1 (MQ=255) cAACCACCGCTTTCGCCTGATCAACGGTACGCACGAACGGGATCATGATCTCAACGTTAGTCa < 1:1206488/63‑1 (MQ=255) cAACCACCGCTTTCGCCTGATCAACGGTACGCACGAACGGGATCATGATCTCAACGTTAGTCa < 1:1252576/63‑1 (MQ=255) cAACCACCGCTTTCGCCTGATCAACGGTACGCACGAACGGGATCATGATCTCAACGTTAGTCa < 1:1665811/63‑1 (MQ=255) cAACCACCGCTTTCGCCTGATCAACGGTACGCACGAACGGGATCATGATCTCAACGTTAGTCa < 1:1736428/63‑1 (MQ=255) cAACCACCGCTTTCGCCTGATCAACGGTACGCACGAACGGGATCATGATCTCAACGTTAGTCa < 1:1839312/63‑1 (MQ=255) cAACCACCGCTTTCGCCTGATCAACGGTACGCACGAACGGGATCATGATCTCAACGTTAGTCa < 1:188405/63‑1 (MQ=255) cAACCACCGCTTTCGCCTGATCAACGGTACGCACGAACGGGATCATGATCTCAACGTTAGTCa < 1:1910734/63‑1 (MQ=255) cAACCACCGCTTTCGCCTGATCAACGGTACGCACGAACGGGATCATGATCTCAACGTTAGTCa < 1:2183962/63‑1 (MQ=255) cAACCACCGCTTTCGCCTGATCAACGGTACGCACGAACGGGATCATGATCTCAACGTTAGTCa < 1:2344671/63‑1 (MQ=255) cAACCACCGCTTTCGCCTGATCAACGGTACGCACGAACGGGATCATGATCTCAACGTTAGTCa < 1:2515422/63‑1 (MQ=255) cAACCACCGCTTTCGCCTGATCAACGGTACGCACGAACGGGATCATGATCTCAACGTTAGTCa < 1:2623971/63‑1 (MQ=255) cAACCACCGCTTTCGCCTGATCAACGGTACGCACGAACGGGATCATGATCTCAACGTTAGTCa < 1:2664366/63‑1 (MQ=255) cAACCACCGCTTTCGCCTGATCAACGGTACGCACGAACGGGATCATGATCTCAACGTTAGTCa < 1:2720124/63‑1 (MQ=255) cAACCACCGCTTTCGCCTGATCAACGGTACGCACGAACGGGATCATGATCTCAACGTTAGTCa < 1:3077796/63‑1 (MQ=255) cAACCACCGCTTTCGCCTGATCAACGGTACGCACGAACGGGATCATGATCTAAACGTTAGTCa < 1:1369199/63‑1 (MQ=255) ccGCTTTCGCCTGATCAACGGTACGCACGAACGGGATCATGATCTCAACGTTAGTCa < 1:1661876/57‑1 (MQ=255) cTTTCGCCTGATCAACGGTACGCACGAACGGTATCATGATCTCAACGTTAGTCa < 1:1628676/54‑1 (MQ=255) cTACGGTACGCACGAACGGGATCATGATCTCAACGTTGGTCAGTCCCATGTCGTTGCGCACACGttt < 1:2140540/67‑1 (MQ=255) cTACGGTACGCACGAACGGGATCATGATCT‑AACGTTGGTCAGTCCCATGTCGtt > 1:113492/1‑54 (MQ=255) | CCCGTTCTCGCCACGTTTCAGCCCCTGACGCGCCAGTTCTTCAACCACCGCTTTCGCCTGATCTACGGTACGCACGAACGGGATCATGATCTCAACGTTGGTCAGTCCCATGTCGTTGCGCACACGTTT > minE/1112788‑1112916 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |