| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,113,361 | A→G | 41.9% | D481D (GAT→GAC) | pps ← | phosphoenolpyruvate synthase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,113,361 | 0 | A | G | 41.9% | 20.1 / 54.9 | 74 | D481D (GAT→GAC) | pps | phosphoenolpyruvate synthase |
| Reads supporting (aligned to +/- strand): ref base A (13/30); new base G (15/16); total (28/46) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.47e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.14e-01 | |||||||||||
GGCGAAGTCGAAAGCACGGTCCGGGTTACCGACGTTCATCATCACTTTCAACGGCAGATCCGGCATCGTTTCTACGCTGGAGCTTTTCACGCTAAATTCCAGCAACTCCGCATAGACGTAACCGG > minE/1113304‑1113428 | ggcGAAGTCGAAAGCACGGTCCGGGTTACCGACGTTCATCATCACTTTCAACGGCAGATCCGGCATCGt < 1:1156858/69‑1 (MQ=255)ggcGAAGTCGAAAGCACGGTCCGGGTTACCGACGTTCATCATCACTTTCAACGGCAGATCCGGCATCGt < 1:2112250/69‑1 (MQ=255)ggcGAAGTCGAAAGCACGGTCCGGGTTACCGACGTTCATCATCACTTTCAACGGCAGATCCGGCATCGt < 1:3342444/69‑1 (MQ=255)ggcGAAGTCGAAAGCACGGTCCGGGTTACCGACGTTCATCATCACTTTCAACGGCAGATCCGGCATCGt < 1:1882391/69‑1 (MQ=255)ggcGAAGTCGAAAGCACGGTCCGGGTTACCGACGTTCATCATCACTTTCAACGGCAGATCCGGCATCGt < 1:2797426/69‑1 (MQ=255)ggcGAAGTCGAAAGCACGGTCCGGGTTACCGACGTTCATCATCACTTTCAACGGCAGATCCGGCATCGt < 1:3165183/69‑1 (MQ=255)ggcGAAGTCGAAAGCACGGTCCGGGTTACCGACGTTCATCATCACTTTCAACGGCAGATCCGGCATCGt < 1:1742403/69‑1 (MQ=255) gcGAAGTCGAAAGCACGGTCCGGGTTACCGACGTTCATCATCACTTTCAACGGCAGATCCGGCATCGtt < 1:475191/69‑1 (MQ=255) gcGAAGTCGAAAGCACGGTCCGGGTTACCGACGTTCATCATCACTTTCAACGGCAGATCCGGCATCGtt < 1:153027/69‑1 (MQ=255) gAAGTCGAAAGCACGGTCCGGGTTACCGACGTTCATCATCACTTTCAACGGCAGATCCGGCATCGTTTc < 1:651237/69‑1 (MQ=255) gAAGTCGAAAGCACGGTCCGGGTTACCGACGTTCATCATCACTTTCAACGGCAGATCCGGCATCGTTTc < 1:452961/69‑1 (MQ=255) gAAGTCGAAAGCACGGTCCGGGTTACCGACGTTCATCATCACTTTCAACGGCAGATCCGGCATCGTTTc < 1:3390048/69‑1 (MQ=255) gAAGTCGAAAGCACGGTCCGGGTTACCGACGTTCATCATCACTTTCAACGGCAGATCCGGCATCGTTTc < 1:218451/69‑1 (MQ=255) aaGTCGAAAGCACGGTCCGGGTTACCGACGTTCATCATCACTTTCAACGGCAGATCCGGCATCGTTTc < 1:1601628/68‑1 (MQ=255) gAAAGCACGGTCCGGGTTACCGATGTTCATCATCACCTTCAACGGCAGGTCCGGCATCGTTTCTACg > 1:2662468/1‑67 (MQ=255) gAAAGCACGGTCCGGGTTACCGACGTTCATCATCACCTTCAACGGCAGGTCCGGCATGGTTTCTACGc > 1:3104207/1‑68 (MQ=255) gAAAGCACGGTCCGGGTTACCGACGTTCATCATCACCTTCAACGGCAGGTCCGGCATCGTTTCTACGc > 1:3078085/1‑68 (MQ=255) gAAAGCACGGTCCGGGTTACCGACGTTCATCATCACCTTCAACGGCAGGTCCGGCATCGTTTCTACGc > 1:1133534/1‑68 (MQ=255) gAAAGCACGGTCCGGGTTACCGACGTTCATCATCACCTTCAACGGCAGGTCCGGCATCGTTTCTACGc > 1:3189165/1‑68 (MQ=255) gAAAGCACGGTCCGGGTTACCGACGTTCATCATCACCTTCAACGGCAGGTCCGGCATCGTTTCTACGc > 1:2621115/1‑68 (MQ=255) gAAAGCACGGTCCGGGTTACCGACGTTCATCATCACCTTCAACGGCAGGTCCGGCATCGTTTCTACGc > 1:3306947/1‑68 (MQ=255) gAAAGCACGGTCCGGGTTACCGACGTTCATCATCACCTTCAACGGCAGGTCCGGCATCGTTTCTACGc > 1:585011/1‑68 (MQ=255) gAAAGCACGGTCCGGGTTACCGACGTTCATCATCACCTTCAACGGCAGGTCCGGCATCGTTTCTACGc > 1:203200/1‑68 (MQ=255) gAAAGCACGGTCCGGGTTACCGACGTTCATCATCACCTTCAACGGCAGGTCCGGCATCGTTTCTACGc > 1:1988479/1‑68 (MQ=255) gAAAGCACGGTCCGGGTTACCGACGTTCATCATCACCTTCAACGGCAGGTCCGGCATCGTTTCTACGc > 1:1483526/1‑68 (MQ=255) gAAAGCACGGTCCGGGTTACCGACGTTCATCATCACCTTCAACGGCAGGTCCGGCATCGTTTCTACGc > 1:1751046/1‑68 (MQ=255) gAAAGCACGGTCCGGGTTACCGACGTTCATCATCACCTTCAACGGCAGGTCCGGCATCGTTTCTACGc > 1:1414268/1‑68 (MQ=255) gAAAGCACGGTCCGGGTTACCGACGTTCATCATCACCTTCAACGGCAGGTCCGGCATCGTTTCTACGc > 1:1352898/1‑68 (MQ=255) gAAAGCACGGTACGGGTTACCGACGTTCATCATCACCTTCAACGGCAGGTCCGGCATCGTTTCTACGc > 1:3280761/1‑68 (MQ=255) ccGGGTTACCGACGTTCATCATCACCTTCAACGGCAGGTCCGGCATCGTTTCTACGCtt < 1:488280/59‑2 (MQ=255) ccGGGTTACCGACGTTCATCATCACCTTCAACGGCAGGTCCGGCATCGTTTCTACGCTg < 1:3306065/59‑1 (MQ=255) ccGGGTTACCGACGTTCATCATCACCTTCAACGGCAGGTCCGGCATCGTTTCTACGCTg < 1:3282898/59‑1 (MQ=255) ccGGGTTACCGACGTTCATCATCACCTTCAACGGCAGGTCCGGCATCGTTTCTACGCTg < 1:1489128/59‑1 (MQ=255) ccGGGTTACCGACGTTCATCATCACCTTCAACGGCAGGTCCGGCATCGTTTCTACGCTg < 1:3328126/59‑1 (MQ=255) ccGGGTTACCGACGTTCATCATCACCTTCAACGGCAGGTCCGGCATCGTTTCTACGCTg < 1:2803214/59‑1 (MQ=255) ccGGGTTACCGACGTTCATCATCACCTTCAACGGCAGGTCCGGCATCGTTTCTACGCTg < 1:1833843/59‑1 (MQ=255) ccGGGTTACCGACGTTCATCATCACCTTCAACGGCAGGTCCGGCATCGTTTCTACGCTg < 1:502102/59‑1 (MQ=255) ccGGGTTACCGACGTTCATCATCACCTTCAACGGCAGGTCCGGCATCGTTTCTACGCTg < 1:1206272/59‑1 (MQ=255) ccGGGTTACCGACGTTCATCATCACCTTCAACGGCAGGTCCGGCATCGTTTCTACGCTg < 1:2618240/59‑1 (MQ=255) ccGGGTTACCGACGTTCATCATCACCTTCAACGGCAGGTCCGGCATCGTTTCTACGCTg < 1:781099/59‑1 (MQ=255) ccGGGTTACCGACGTTCATCATCACCTTCAACGGCAGGTCCGGCATCGTTTCTACGCTg < 1:1953006/59‑1 (MQ=255) ccGGGTTACCGACGTTCATCATCACCTTCAACGGCAGGTCCGGCATCGTTTCTACGCTg < 1:91073/59‑1 (MQ=255) ccGGGTTACCGACGTTCATCATCACCTTCAACGGCAGGTCCGGCATCGTTTCTACGCTg < 1:2092723/59‑1 (MQ=255) cGGGTTACCGACGTTCATCATCACCTTCAACGGCAGGTCCGTCATCGTTTCTACGCTg < 1:148864/58‑1 (MQ=255) cGGGTTACCGACGTTCATCATCACCTTCAACGGCAGGTCCGGCATCGTTTCTACGCTg < 1:3005303/58‑1 (MQ=255) gTTCATCATCACTTTCAACGGCAGATCCGGCATCGTTTCTACGCTGGAGCTTTTCACGCTAAATTCCa > 1:306744/1‑68 (MQ=255) catcatcaCTTTCAACGGCAGATCCGGCATCGTTTCTACGCTGGAGCTTTTCACGCTAAATTCCAGCaa > 1:35854/1‑69 (MQ=255) catcatcaCTTTCAACGGCAGATCCGGCATCGTTTCTACGCTGGAGCTTTTCACGCTAAATTCCAGCaa > 1:910285/1‑69 (MQ=255) caCTTTCAACGGCAGATCCGGCATCGTTTCTACGCTGGAGCTTTTCACGCTAAATTCCAGCAACTCCg < 1:654221/68‑1 (MQ=255) caCTTTCAACGGCAGATCCGGCATCGTTTCTACGCTGGAGCTTTTCACGCTAAATTCCAGCAACTCCg < 1:894471/68‑1 (MQ=255) caCTTTCAACGGCAGATCCGGCATCGTTTCTACGCTGGAGCTTTTCACGCTAAATTCCAGCAACTCCg < 1:3330957/68‑1 (MQ=255) caCTTTCAACGGCAGATCCGGCATCGTTTCTACGCTGGAGCTTTTCACGCTAAATTCCAGCAACTCCg < 1:947814/68‑1 (MQ=255) caCTTTCAACGGCAGATCCGGCATCGTTTCTACGCTGGAGCTTTTCACGCTAAATTCCAGCAACTCCg < 1:1557132/68‑1 (MQ=255) caCTTTCAACGGCAGATCCGGCATCGTTTCTACGCTGGAGCTTTTCACGCTAAATTCCAGCAACTCCg < 1:1665190/68‑1 (MQ=255) caCTTTCAACGGCAGATCCGGCATCGTTTCTACGCTGGAGCTTTTCACGCTAAATTCCAGCAACTCCg > 1:231583/1‑68 (MQ=255) caCTTTCAACGGCAGATCCGGCATCGTTTCTACGCTGGAGCTTTTCACGCTAAATTCCAGCAACTCCg < 1:2980504/68‑1 (MQ=255) caCTTTCAACGGCAGATCCGGCATCGTTTCTACGCTGGAGCTTTTCACGCTAAATTCCAGCAACTCCg > 1:2317721/1‑68 (MQ=255) caCTTTCAACGGCAGATCCGGCATCGTTTCTACGCTGGAGCTTTTCACGCTAAATTCCAGCAACTCCg < 1:2688601/68‑1 (MQ=255) caCTTTCAACGGCAGATCCGGCATCGTTTCTACGCTGGAGCTTTTCACGCTAAATTCCAGCAACTCCg < 1:2674673/68‑1 (MQ=255) caCTTTCAACGGCAGATCCGGCATCGTTTCTACGCTGGAGCTTTTCACGCTAAATTCCAGCAACTCCg < 1:3225511/68‑1 (MQ=255) caCTTTCAACGGCAGATCCGGCATCGTTTCTACGCTGGAGCTTTTCACGCTAAATTCCAGCAACTCCg > 1:1931398/1‑68 (MQ=255) aCTTTCAACGGCAGATCCGGCATCGTTTCTACGCTGGAGCtt > 1:1957813/1‑42 (MQ=255) aCTTTCAACGGCAGATCCGGCATCGTTTCTACGCTGGAGCtt > 1:775271/1‑42 (MQ=255) aCTTTCAACGGCAGATCCGGCATCGTTTCTACGCTGGAGCtt > 1:1114822/1‑42 (MQ=255) aCTTTCAACGGCAGATCCGGCATCGTTTCTACGCTGGAGCTTTTCACGCTAAATTCCAGCAACTCCg > 1:560677/1‑67 (MQ=255) aCTTTCAACGGCAGATCCGGCATCGTTTCTACGCTGGAGCTTTTCACGCTAAATTCCAGCAACTCCg > 1:866856/1‑67 (MQ=255) aCTTTCAACGGCAGATCCGGCATCGTTTCTACGCTGGAGCTTTTCACGCTAAATTCCAGCAACTCCg > 1:245195/1‑67 (MQ=255) aCTTTCAACGGCAGATCCGGCATCGTTTCTACGCTGGAGCTTTTCACGCTAAATTCCAGCAACTCCg > 1:2630235/1‑67 (MQ=255) tttCAACGGCAGATCCGGCATCGTTTCTACGCTGGAGCTTTTCACGCTAAATTCCAGCAACTCCGCATa < 1:2917393/69‑1 (MQ=255) tttCAACGGCAGATCCGGCATCGTTTCTACGCTGGAGCTTTTCACGCTAAATTCCAGCAACTCCGCATa < 1:3098133/69‑1 (MQ=255) ttCAACGGCAGATCCGGCATCGTTTCTACGCTGGAGCTTTTCACGCTAAATTCCAGCAACTCCGCATa < 1:1684667/68‑1 (MQ=255) cAGATCCGGCATCGTTTCTACGCTGGAGCTTTTCACGCTAAATTCCAGCAACTCCg < 1:1834251/56‑1 (MQ=255) gATCCGGCATCGTTTCTACGCTGGAGCTTTTCACGCTAAATTCCAGCAACTCCGCATAGACGTAACCgg < 1:380918/69‑1 (MQ=255) gATCCGGCATCGTTTCTACGCTGGAGCTTTTCACGCTAAATTCCAGCAACTCCGCATAGACGTAACCgg < 1:2031060/69‑1 (MQ=255) | GGCGAAGTCGAAAGCACGGTCCGGGTTACCGACGTTCATCATCACTTTCAACGGCAGATCCGGCATCGTTTCTACGCTGGAGCTTTTCACGCTAAATTCCAGCAACTCCGCATAGACGTAACCGG > minE/1113304‑1113428 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |