| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,114,060 | C→A | 45.7% | L248L (CTG→CTT) | pps ← | phosphoenolpyruvate synthase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,114,060 | 0 | C | A | 45.7% | 6.5 / 33.5 | 35 | L248L (CTG→CTT) | pps | phosphoenolpyruvate synthase |
| Reads supporting (aligned to +/- strand): ref base C (17/2); new base A (10/6); total (27/8) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.05e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.53e-01 | |||||||||||
CATGCGGATTTTTTTCGACCCCATGGTGCGGCGCACGATAGCCGGGCGATTCGCCGCCAGTGTCGGTTTATGCACGTAAAACTCATCCGGGTTAACCGCACCCTGCACGACCATCTCA > minE/1114003‑1114120 | cATGCGGATTTTTTTCGACCCCATGGTGCGGCGCACGATAGCCGGGCGATTCGCCGCCAGTGTCGGttt > 1:1133358/1‑69 (MQ=255) aTGCGGATTTTTTTCGACCCCATGGTGCGGCGCACAATAGCCGGGCGATTCGCCGCAAGTGTCGGTTTa > 1:2620023/1‑69 (MQ=255) aTGCGGATTTTTTTCGACCCCATGGTGCGGCGCACAATAGCCGGGCGATTCGCCGCAAGTGTCGGTTTa > 1:453238/1‑69 (MQ=255) aTGCGGATTTTTTTCGACCCCATGGTGCGGCGCACAATAGCCGGGCGATTCGCCGCAAGTGTCGGTTTa > 1:3377156/1‑69 (MQ=255) aTGCGGATTTTTTTCGACCCCATGGTGCGGCGCACAATAGCCGGGCGATTCGCCGCAAGTGTCGGTTTa > 1:2989575/1‑69 (MQ=255) aTGCGGATTTTTTTCGACCCCATGGTGCGGCGCACAATAGCCGGGCGATTCGCCGCAAGTGTCGGTTTa > 1:2751304/1‑69 (MQ=255) aTGCGGATTTTTTTCGACCCCATGGTGCGGCGCACAATAGCCGGGCGATTCGCCGCAAGTGTCGGTTTa > 1:2718366/1‑69 (MQ=255) aTGCGGATTTTTTTCGACCCCATGGTGCGGCGCACAATAGCCGGGCGATTCGCCGCAAGTGTCGGTTTa > 1:2447483/1‑69 (MQ=255) aTGCGGATTTTTTTCGACCCCATGGTGCGGCGCACAATAGCCGGGCGATTCGCCGCAAGTGTCGGTTTa > 1:1556679/1‑69 (MQ=255) aTGCGGATTTTTTTCGACCCCATGGTGCGGCGCACAATAGCCGGGCGATTCGCCGCAAGTGTCGGTTTa > 1:1667491/1‑69 (MQ=255) aTGCGGATTTTTTTAGACCCCATGGTGCGGCGCAAAATAGCCGGGCGATTCGCAGCAAGTGTCGGttt > 1:2762440/1‑68 (MQ=255) ggTGCGGCGCACGATAGCCGGGCGATTCGCCGCCAGTGTCGGTTTATGCACGTAAAACTCATCCGGGtt > 1:685895/1‑69 (MQ=255) ggTGCGGCGCACGATAGCCGGGCGATTCGCCGCCAGTGTCGGTTTATGCACGTAAAACTCATCCGGGtt > 1:1340944/1‑69 (MQ=255) ggTGCGGCGCACGATAGCCGGGCGATTCGCCGCCAGTGTCGGTTTATGCACGTAAAACTCATCCGGGtt > 1:1404951/1‑69 (MQ=255) ggTGCGGCGCACGATAGCCGGGCGATTCGCCGCCAGTGTCGGTTTATGCACGTAAAACTCATCCGGGtt > 1:3060073/1‑69 (MQ=255) ggTGCGGCGCACAATAGCCGGGCGATTCGCCGCAAGTGTCGGTTTATGCACATAAAACTCATCCTGGtt < 1:2503330/69‑1 (MQ=255) ggTGCGGCGCACAATAGCCGGGCGATTCGCCGCAAGTGTCGGTTTATGCACATAAAACTCATCCGGGtt < 1:2171284/69‑1 (MQ=255) ggTGCGGCGCACAATAGCCGGGCGATTCGCCGCAAGTGTCGGTTTATGCACATAAAACTCATCCGGGtt < 1:3202119/69‑1 (MQ=255) ggTGCGGCGCACAATAGCCGGGCGATTCGCCGCAAGTGTCGGTTTATGCACATAAAACTCATCCGGGtt < 1:2886849/69‑1 (MQ=255) ggTGCGGCGCACAATAGCCGGGCGATTCGCCGCAAGTGTCGGTTTATGCACATAAAACTCATCCGGGtt < 1:275483/69‑1 (MQ=255) ggTGCGGCGCACAATAGCCGGGCGATTCGCCGCAAGTGTCGGTTTATGCACATAAAACTCATCCGGGtt < 1:2499302/69‑1 (MQ=255) cACGATAGCCGGGCGATTCGCCGCCAGTGTCGGTTTATGCACGTAAAACTCATCCGGGTTAACCGCAcc > 1:1120909/1‑69 (MQ=255) cACGATAGCCGGGCGATTCGCCGCCAGTGTCGGTTTATGCACGTAAAACTCATCCGGGTTAACCGCAcc > 1:3033651/1‑69 (MQ=255) cACGATAGCCGGGCGATTCGCCGCCAGTGTCGGTTTATGCACGTAAAACTCATCCGGGTTAACCGCAcc > 1:3121984/1‑69 (MQ=255) cACGATAGCCGGGCGATTCGCCGCCAGTGTCGGTTTATGCACGTAAAACTCATCCGGGTTAACCGCAcc > 1:2008341/1‑69 (MQ=255) gCCGGGCGATTCGCCGCCAGTGTCGGTTTATGCACGTAAAACTCATCCGGGTTAACCGCACCCTGCACg > 1:729751/1‑69 (MQ=255) tCGCCGCCAGTGTCGGTTTATGCACGTAAAACTCATCCGGGTTAACCGCa < 1:2296728/50‑1 (MQ=255) tCGCCGCCAGTGTCGGTTTATGCACGTAAAACTCATCCGGGTTAACCGCa < 1:2228181/50‑1 (MQ=255) tCGCCGCCAGTGTCGGTTTATGCACGTAAAACTCATCCGGGTTAACCGCACCCTGCACGACCATCTCa > 1:1637574/1‑68 (MQ=255) tCGCCGCCAGTGTCGGTTTATGCACGTAAAACTCATCCGGGTTAACCGCACCCTGCACGACCATCTCa > 1:245147/1‑68 (MQ=255) tCGCCGCCAGTGTCGGTTTATGCACGTAAAACTCATCCGGGTTAACCGCACCCTGCACGACCATCTCa > 1:1715707/1‑68 (MQ=255) tCGCCGCCAGTGTCGGTTTATGCACGTAAAACTCATCCGGGTTAACCGCACCCTGCACGACCATCTCa > 1:2301383/1‑68 (MQ=255) tCGCCGCCAGTGTCGGTTTATGCACGTAAAACTCATCCGGGTTAACCGCACCCTGCACGACCATCTCa > 1:530686/1‑68 (MQ=255) tCGCCGCCAGTGTCGGTTTATGCACGTAAAACTCATCCGGGTTAACCGCACCCTGCACGACCATCTCa > 1:901100/1‑68 (MQ=255) tCGCCGCCAGTGTCGGTTTATGCACGTAAAACTCATCCGGGTAAACCGCACCCTGCACGACCATCTCa > 1:3107696/1‑68 (MQ=255) | CATGCGGATTTTTTTCGACCCCATGGTGCGGCGCACGATAGCCGGGCGATTCGCCGCCAGTGTCGGTTTATGCACGTAAAACTCATCCGGGTTAACCGCACCCTGCACGACCATCTCA > minE/1114003‑1114120 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |