| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,114,225 | T→C | 33.3% | Q193Q (CAA→CAG) | pps ← | phosphoenolpyruvate synthase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,114,225 | 0 | T | C | 33.3% | 59.8 / 43.5 | 81 | Q193Q (CAA→CAG) | pps | phosphoenolpyruvate synthase |
| Reads supporting (aligned to +/- strand): ref base T (23/31); new base C (17/10); total (40/41) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.02e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.95e-01 | |||||||||||
TCGGTATCAATGGAGAACATCACGCCAGATGATGCGAGGTCAGAGCGCACCATCCGTTGAACACCGGCGGAGAGCGCCACACCACGGTGATCGTAACCCTGGTGCACACGATAAGAGATGGC > minE/1114169‑1114290 | tCGGTATCAATGGAGAACATCACGCCAGATGATGCGAGGTCAGAGCGCACCATCCGTTGAACACcggcg < 1:1634888/69‑1 (MQ=255) aaTGGAGAACATCACGCCAGATGATGCGAGGTCAGAGCGCACCATCCGTTGAACACCGGCGGAGAgcgc > 1:419609/1‑69 (MQ=255) aaTGGAGAACATCACGCCAGATGATGCGAGGTCAGAGCGCACCATCCGTTGAACACCGGCGGAGAgcgc > 1:2446712/1‑69 (MQ=255) aaTGGAGAACATCACGCCAGATGATGCGAGGTCAGAGCGCACCATCCGTTGAACACCGGCGGAGAgcgc > 1:681839/1‑69 (MQ=255) aaTGGAGAACATCACGCCAGATGATGCGAGGTCAGAGCGCACCATCCGTTGAACACCGGCGGAGAgcgc > 1:3154038/1‑69 (MQ=255) aaTGGAGAACATCACGCCAGATGATGCGAGGTCAGAGCGCACCATCCGTTGAACACCGGCGGAGAgcgc > 1:331750/1‑69 (MQ=255) aaTGGAGAACATCACGCCAGATGATGCGAGGTCAGAGCGCACCATCCGTTGAACACCGGCGGAGAgcgc > 1:3409159/1‑69 (MQ=255) cATCACGCCAGATGATGCGAGGTCAGAGCGCACCATCCGTTGAACACCGGCGGAGAGCGCCACACCACg > 1:1288593/1‑69 (MQ=255) cATCACGCCAGATGATGCGAGGTCAGAGCGCACCATCCGTTGAACACCGGCGGAGAGCGCCACACCACg > 1:3396280/1‑69 (MQ=255) cATCACGCCAGATGATGCGAGGTCAGAGCGCACCATCCGTTGAACACCGGCGGAGAGCGCCACACCACg > 1:1205619/1‑69 (MQ=255) cATCACGCCAGATGATGCGAGGTCAGAGCGCACCATCCGTTGAACACCGGCGGAGAGCGCCACACCACg > 1:792543/1‑69 (MQ=255) cATCACGCCAGATGATGCGAGGTCAGAGCGCACCATCCGTTGAACACCGGCGGAGAGCGCCACACCACg > 1:2100285/1‑69 (MQ=255) aTCACGCCAGATGATGCGAGGTCAGAGCGCACCATCCGTTGAACACCGGCGGAGAGCGCCACACCACg > 1:329605/1‑68 (MQ=255) aTCACGCCAGATGATGCGAGGTCAGAGCGCACCATCCGTTGAACACCGGCGGAGAGCGCCACACCACg > 1:2915385/1‑68 (MQ=255) aTCACGCCAGATGATGCGAGGTCAGAGCGCACCATCCGTTGAACACCGGCGGAGAGCGCCACACCACg > 1:927036/1‑68 (MQ=255) aTCACGCCAGATGATGCGAGGTCAGAGCGCACCATCCGTTGAACACCGGCGGAGAGCGCCACACCACg > 1:2022901/1‑68 (MQ=255) cACGCCAGATGATGCGAGGTCAGAGCGCACCATCCGTTGAACACCGGCGGAGAGCGCCGCACCACGGTg < 1:1240062/69‑1 (MQ=255) cACGCCAGATGATGCGAGGTCAGAGCGCACCATCCGTTGAACACCGGCGGAGAGCGCCACACCACGGTg < 1:337613/69‑1 (MQ=255) cACGCCAGATGATGCGAGGTCAGAGCGCACCATCCGTTGAACACCGGCGGAGAGCGCCACACCACGGTg < 1:64058/69‑1 (MQ=255) cACGCCAGATGATGCGAGGTCAGAGCGCACCATCCGTTGAACACCGGCGGAGAGCGCCACACCACGGTg < 1:287491/69‑1 (MQ=255) cACGCCAGATGATGCGAGGTCAGAGCGCACCATCCGTTGAACACCGGCGGAGAGCGCCACACCACGGTg < 1:2421077/69‑1 (MQ=255) cACGCCAGATGATGCGAGGTCAGAGCGCACCATCCGTTGAACACCGGCGGAGAGCGCCACACCACGGTg < 1:2198786/69‑1 (MQ=255) cACGCCAGATGATGCGAGGTCAGAGCGCACCATCCGTTGAACACCGGCGGAGAGCGCCACACCACGGTg < 1:1822022/69‑1 (MQ=255) cACGCCAGATGATGCGAGGTCAGAGCGCACCATCCGTTGAACACCGGCGGAGAGCGCCACACCACGGTg < 1:1513265/69‑1 (MQ=255) cACGCCAGATGATGCGAGGTCAGAGCGCACCATCCGTTGAACACCGGCGGAGAGCGCCACACCACGGTg < 1:1181794/69‑1 (MQ=255) cACGCCAGAAGATGCGAGGTCGGAGCGCACCATCCGCTGAACACCGGCGGAGAGCGCTACGCCACGATg < 1:975178/69‑1 (MQ=255) cACGCCAGAAGATGCGAGGTCGGAGCGCACCATCCGCTGAACACCGGCGGAGAGCGCTACGCCACGATg < 1:3247734/69‑1 (MQ=255) cACGCCAGAAGATGCGAGGTCGGAGCGCACCATCCGCTGAACACCGGCGGAGAGCGCTACGCCACGATg < 1:3134574/69‑1 (MQ=255) cACGCCAGAAGATGCGAGGTCGGAGCGCACCATCCGCTGAACACCGGCGGAGAGCGCTACGCCACGATg < 1:987955/69‑1 (MQ=255) cACGCCAGAAGATGCGAGGTCGGAGCGCACCATCCGCTGAACACCGGCGGAGAGCGCTACGCCACGATg < 1:3015331/69‑1 (MQ=255) cACGCCAGAAGATGCGAGGTCGGAGCGCACCATCCGCTGAACACCGGCGGAGAGCGCTACGCCACGATg < 1:1552701/69‑1 (MQ=255) cACGCCAGAAGATGCGAGGTCGGAGCGCACCATCCGCTGAACACCGGCGGAGAGCGCTACGCCACGATg < 1:3323777/69‑1 (MQ=255) cACGCCAGAAGATGCGAGGTCGGAGCGCACCATCCGCTGAACACCGGCGGAGAGCGCTACGCCACGATg < 1:455576/69‑1 (MQ=255) cACGCCAGAAGATGCGAGGTCGGAGCGCACCATCCGCTGAACACCGGCGGAGAGCGCTACGCCACGATg < 1:2276939/69‑1 (MQ=255) cACGCCAGAAGATGCGAGGTCGGAGCGCACCATCCGCTGAACACCGGCGGAGAGCGCTACGCCACGATg < 1:423206/69‑1 (MQ=255) aCGCCAGATGATGCGATGTCAGAGCGCACCATCCGTTGAACACCGGCGGAGAGCGCCACACCACGGTg < 1:825291/68‑1 (MQ=255) aCGCCAGATGATGCGAGGTCAGAGCGCACCATCCGTTGAACACCGGCGGAGAGCGCCACACCACGGTg < 1:2871786/68‑1 (MQ=255) tgatgCGAGGTCAGAGCGCACCATCCGTTGAACACCGGCGGAGAGCGCCACACCACGGTGATCGTa < 1:779707/66‑1 (MQ=255) tgatgCGAGGTCAGAGCGCACCATCCGTTGAACACCGGCGGAGAGCGCCACACCACGGTGATCGTa < 1:3339457/66‑1 (MQ=255) tgatgCGAGGTCAGAGCGCACCATCCGTTGAACACCGGCGGAGAGCGCCACACCACGGTGATCGTa < 1:81802/66‑1 (MQ=255) tgatgCGAGGTCAGAGCGCACCATCCGTTGAACACCGGCGGAGAGCGCCACACCACGGTGATCGTa < 1:1772809/66‑1 (MQ=255) gatgCGAGGTCAGAGCGCACCATCCGTTGAACACCGGCGGAGAGCGCCACACCACGGTGATCGTa < 1:45211/65‑1 (MQ=255) ggagCGCACCATCCGCTGAACACCGGCGGAGAGCGCTACGCCACGATGGTCGTAACCCTGGTGCacac > 1:1240889/2‑68 (MQ=255) ggagCGCACCATCCGCTGAACACCGGCGGAGAGCGCTACGCCACGATGGTCGTAACCCTGGTGCacac > 1:3324753/2‑68 (MQ=255) ggagCGCACCATCCGCTGAACACCGGCGGAGAGCGCTACGCCACGATGGTCGTAACCCTGGTGCacac > 1:405293/2‑68 (MQ=255) ggagCGCACCATCCGCTGAACACCGGCGGAGAGCGCTACGCCACGATGGTCGTAACCCTGGTGCacac > 1:1420057/2‑68 (MQ=255) ggagCGCACCATCCGCTGAACACCGGCGGAGAGCGCTACGCCACGATGGTCGTAACCCTGGTGCacac > 1:1998210/2‑68 (MQ=255) ggagCGCACCATCCGCTGAACACCGGCGGAGAGCGCTACGCCACGATGGTCGTAACCCTGGTGCacac > 1:1163883/2‑68 (MQ=255) ggagCGCACCATCCGCTGAACACCGGCGGAGAGCGCTACGCCACGATGGTCGTAACCCTGGTGCacac > 1:663800/2‑68 (MQ=255) ggagCGCACCATCCGCTGAACACCGGCGGAGAGCGCTACGCCACGATGGTCGTAACCCTGGTGCacac > 1:3044785/2‑68 (MQ=255) ggagCGCACCATCCGCTGAACACCGGCGGAGAGCGCTACGCCACGATGGTCGTAACCCTGGTGCacac > 1:739486/2‑68 (MQ=255) ggagCGCACCATCCGCTGAACACCGGCGGAGAGCGCTACGCCACGATGGTCGTAACCCTGGTGCacac > 1:963935/2‑68 (MQ=255) ggagCGCACCATCCGCTGAACACCGGCGGAGAGCGCTACGCCACGATGGTCGTAACCCTGGTGCacac > 1:1621203/2‑68 (MQ=255) ggagCGCACCATCCGCTGAACACCGGCGGAGAGCGCTACGCCACGATGGTCGTAACCCTGGTGCacac > 1:2645514/2‑68 (MQ=255) ggagCGCACCATCCGCTGAACACCGGCGGAGAGCGCTACGCCACGATGGTCGTAACCCTGGTGCacac > 1:84192/2‑68 (MQ=255) ggagCGCACCATCCGCTGAACACCGGCGGAGAGCGCTACGCCACGATGGTCGTAACCCTGGTGCacac > 1:1677959/2‑68 (MQ=255) ggagCGCACCATCCGCTGAACACCGGCGGAGAGCGCTACGCCACGATGGTCGTAACCCTGGTGCacac > 1:2274438/2‑68 (MQ=255) ggagCGCACCATCCGCTGAACACCGGCGGAGAGCGCTACGCCACGATGGTCGTAACCCTGGTGCacac > 1:2162066/2‑68 (MQ=255) ggagCGCACCATCCGCTGAACACCGGCGGAGAGCGCTACGCCACGATGGTCGTAACCCTGGTGCacac > 1:1818891/2‑68 (MQ=255) cACCATCCGTTGAACACCGGCGGAGAGCGCCACACCACGGTGATCGTAACCCTGGTGCACACGATAaga < 1:465043/69‑1 (MQ=255) cACCATCCGTTGAACACCGGCGGAGAGCGCCACACCACGGTGATCGTAACCCTGGTGCACACGATAaga < 1:1087571/69‑1 (MQ=255) cACCATCCGTTGAACACCGGCGGAGAGCGCCACACCACGGTGATCGTAACCCTGGTGCACACGATAaga < 1:99443/69‑1 (MQ=255) cACCATCCGTTGAACACCGGCGGAGAGCGCCACACCACGGTGATCGTAACCCTGGTGCACACGATAaga < 1:3339911/69‑1 (MQ=255) cACCATCCGTTGAACACCGGCGGAGAGCGCCACACCACGGTGATCGTAACCCTGGTGCACACGATAaga < 1:3311775/69‑1 (MQ=255) cACCATCCGTTGAACACCGGCGGAGAGCGCCACACCACGGTGATCGTAACCCTGGTGCACACGATAaga < 1:1406038/69‑1 (MQ=255) cACCATCCGTTGAACACCGGCGGAGAGCGCCACACCACGGTGATCGTAACCCTGGTGCACACGATAaga < 1:3264014/69‑1 (MQ=255) cACCATCCGTTGAACACCGGCGGAGAGCGCCACACCACGGTGATCGTAACCCTGGTGCACACGATAaga < 1:3004421/69‑1 (MQ=255) cACCATCCGTTGAACACCGGCGGAGAGCGCCACACCACGGTGATCGTAACCCTGGTGCACACGATAaga < 1:1576788/69‑1 (MQ=255) cACCATCCGTTGAACACCGGCGGAGAGCGCCACACCACGGTGATCGTAACCCTGGTGCACACGATAaga < 1:2588815/69‑1 (MQ=255) cACCATCCGTTGAACACCGGCGGAGAGCGCCACACCACGGTGATCGTAACCCTGGTGCACACGATAaga < 1:2396964/69‑1 (MQ=255) cACCATCCGTTGAACACCGGCGGAGAGCGCCACACCACGGTGATCGTAACCCTGGTGCACACGATAaga < 1:220665/69‑1 (MQ=255) cACCATCCGTTGAACACCGGCGGAGAGCGCCACACCACGGTGATCGTAACCCTGGTGCACACGATAaga < 1:1649152/69‑1 (MQ=255) ccATCCGTTGAACACGGGCGGAGAGCGCCACACCACGGTGATCGTa < 1:2604412/46‑1 (MQ=255) cGTTGAACACCGGCGGAGAGCGCCACACCACGGTGATCGTAACCCTGGTGCACACGATAAGAGATGgc > 1:1364511/1‑68 (MQ=255) cGTTGAACACCGGCGGAGAGCGCCACACCACGGTGATCGTAACCCTGGTGCACACGATAAGAGATGgc > 1:344422/1‑68 (MQ=255) cGTTGAACACCGGCGGAGAGCGCCACACCACGGTGATCGTAACCCTGGTGCACACGATAAGAGATGgc > 1:1460464/1‑68 (MQ=255) cGTTGAACACCGGCGGAGAGCGCCACACCACGGTGATCGTAACCCTGGTGCACACGATAAGAGATGgc > 1:1634935/1‑68 (MQ=255) cGTTGAACACCGGCGGAGAGCGCCACACCACGGTGATCGTAACCCTGGTGCACACGATAAGAGATGgc > 1:2963740/1‑68 (MQ=255) cGTTGAACACCGGCGGAGAGCGCCACACCACGGTGATCGTAACCCTGGTGCACACGATAAGAGATGgc > 1:3220545/1‑68 (MQ=255) cGTTGAACACCGGCGGAGAGCGCCACACCACGGTGATCGTAACCCTGGTGCACACGATAAGAGATGgc > 1:50272/1‑68 (MQ=255) cGTTGAACACCGGCGGAGAGCGCCACACCACGGTGATCGTAACCCTGGTGCACACGATAAGAGATGgc > 1:3356651/1‑68 (MQ=255) | TCGGTATCAATGGAGAACATCACGCCAGATGATGCGAGGTCAGAGCGCACCATCCGTTGAACACCGGCGGAGAGCGCCACACCACGGTGATCGTAACCCTGGTGCACACGATAAGAGATGGC > minE/1114169‑1114290 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |