| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,117,194 | A→G | 54.8% | intergenic (+22/‑110) | aroH → / → ydiE | 3‑deoxy‑D‑arabino‑heptulosonate‑7‑phosphate synthase, tryptophan repressible/conserved hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,117,194 | 0 | A | G | 54.8% | 7.7 / 40.7 | 53 | intergenic (+22/‑110) | aroH/ydiE | 3‑deoxy‑D‑arabino‑heptulosonate‑7‑phosphate synthase, tryptophan repressible/conserved hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base A (13/11); new base G (12/17); total (25/28) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.15e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.69e-01 | |||||||||||
TCGAAAAACTCGCCTCTGCGGTAGATACCCGCTTCTGAATGCGTGCCCATTCCTGACGGAATGGGCATTTCTGCGCAACTTGTTGTCTTCTCAACAAATTACTGCTTGCTCTGGTCAGC > minE/1117135‑1117253 | tCGAAAAACTCGCCTCTGCGGTAGATACTCGCTTCTGAATGCGTGCCCATTCCTGACGGGATGGGCAtt > 1:274560/1‑69 (MQ=255)tCGAAAAACTCGCCTCTGCGGTAGATACTCGCTTCTGAATGCGTGCCCATTCCTGACGGGATGGGCAtt > 1:1793705/1‑69 (MQ=255)tCGAAAAACTCGCCTCTGCGGTAGATACTCGCTTCTGAATGCGTGCCCATTCCTGACGGGATGGGCAtt > 1:686790/1‑69 (MQ=255) ctctGCGGTAGATACCCGCTTCTGAATGCGTGCCCATTCCTGACGGAATGGGCATTTCTGCGCAACtt < 1:311834/68‑1 (MQ=255) gTAGATACCCGCTTCTGAATGCGTGCCCATTCCTGACGGAATGGGCATTTCTGCGCAACTtgttgt < 1:997972/66‑1 (MQ=255) gTAGATACCCGCTTCTGAATGCGTGCCCATTCCTGACGGAATGGGCATTTCTGCGCAACTTGTTGtctt < 1:2933069/69‑1 (MQ=255) gTAGATACCCGCTTCTGAATGCGTGCCCATTCCTGACGGAATGGGCATTTCTGCGCAACTTGTTGtctt < 1:2218834/69‑1 (MQ=255) aGATACCCGCTTCTGAATGCGTGCCCATTCCTGACGGAATGGGCATTTCTGCGCAACTTGTTGtctt < 1:1547699/67‑1 (MQ=255) aGATACCCGCTTCTGAATGCGTGCCCATTCCTGACGGAATGGGCATTTCTGCGCAACTTGTTGtctt < 1:1040529/67‑1 (MQ=255) aGATACCCGCTTCTGAATGCGTGCCCATTCCTGACGGAATGGGCATTTCTGCGCAACTTGTTGtctt < 1:3287445/67‑1 (MQ=255) aGATACCCGCTTCTGAATGCGTGCCCATTCCTGACGGAATGGGCATTTCTGCGCAACTTGTTGtctt < 1:95036/67‑1 (MQ=255) tACCCGCTTCTGAATGCGTGCCCATTCCTGACGGAATGGGCAtt > 1:2760900/1‑44 (MQ=255) tACCCGCTTCTGAATGCGTGCCCATTCCTGACGGAATGGGCATTTCTGCGCAACTTGTTGTCTTCTca > 1:2648179/1‑68 (MQ=255) tACCCGCTTCTGAATGCGTGCCCATTCCTGACGGAATGGGCATTTCTGCGCAACTTGTTGTCTTCTca > 1:422511/1‑68 (MQ=255) tACCCGCTTCTGAATGCGTGCCCATTCCTGACGGAATGGGCATTTCTGCGCAACTTGTTGTCTTCTca > 1:2439024/1‑68 (MQ=255) tACCCGCTTCTGAATGCGTGCCCATTCCTGACGGAATGGGCATTTCTGCGCAACTTGTTGTCTTCTca > 1:2763845/1‑68 (MQ=255) tACCCGCTTCTGAATGCGTGCCCATTCCTGACGGAATGGGCATTTCTGCGCAACTTGTTGTCTTCTca > 1:2060984/1‑68 (MQ=255) tACCCGCTTCTGAATGCGTGCCCATTCCTGACGGAATGGGCATTTCTGCGCAACTTGTTGTCTTCTca > 1:2150223/1‑68 (MQ=255) tACCCGCTTCTGAATGCGTGCCCATTCCTGACGGAATGGGCAGTTCTGCGCAACTtgttgt > 1:3107819/1‑61 (MQ=255) ttCTGAATGCGTGCCAATTCCTGACGGAATGGGCATTTCTGCGCAACTTGTTGtctt < 1:391345/57‑1 (MQ=255) gAATGCGTGCCCATTCCTGACGGGATGGGCATTTCTGCGCAACTTGTTGTCTTCTCAACAAATTACta < 1:3035213/68‑2 (MQ=255) gAATGCGTGCCCATTCCTGACGGGATGGGCATTTCTGCGCAACTTGTTGTCTTCTCAACAAATTACta < 1:7210/68‑2 (MQ=255) gAATGCGTGCCCATTCCTGACGGGATGGGCATTTCTGCGCAACTTGTTGTCTTCTCAACAAATTACta < 1:2915423/68‑2 (MQ=255) gAATGCGTGCCCATTCCTGACGGGATGGGCATTTCTGCGCAACTTGTTGTCTTCTCAACAAATTACta < 1:3180966/68‑2 (MQ=255) gAATGCGTGCCCATTCCTGACGGGATGGGCATTTCTGCGCAACTTGTTGTCTTCTCAACAAATTACta < 1:722004/68‑2 (MQ=255) gAATGCGTGCCCATTCCTGACGGGATGGGCATTTCTGCGCAACTTGTTGTCTTCTCAACAAATTACta < 1:3391082/68‑2 (MQ=255) gAATGCGTGCCCATTCCTGACGGGATGGGCATTTCTGCGCAACTTGTTGTCTTCTCAACAAATTACta < 1:264578/68‑2 (MQ=255) gAATGCGTGCCCATTCCTGACGGGATGGGCATTTCTGCGCAACTTGTTGTCTTCTCAACAAATTACta < 1:472965/68‑2 (MQ=255) gAATGCGTGCCCATTCCTGACGGGATGGGCATTTCTGCGCAACTTGTTGTCTTCTCAACAAATTACta < 1:2380434/68‑2 (MQ=255) gAATGCGTGCCCATTCCTGACGGGATGGGCATTTCTGCGCAACTTGTTGTCTTCTCAACAAATTACta < 1:2244428/68‑2 (MQ=255) gAATGCGTGCCCATTCCTGACGGGATGGGCATTTCTGCGCAACTTGTTGTCTTCTCAACAAATTACta < 1:2229450/68‑2 (MQ=255) gAATGCGTGCCCATTCCTGACGGGATGGGCATTTCTGCGCAACTTGTTGTCTTCTCAACAAATTACta < 1:1069338/68‑2 (MQ=255) gAATGCGTGCCCATTCCTGACGGGATGGGCATTTCTGCGCAACTTGTTGTCTTCTCAACAAATTACta < 1:2096253/68‑2 (MQ=255) gAATGCGTGCCCATTCCTGACGGGATGGGCATTTCTGCGCAACTTGTTGTCTTCTCAACAAATTACta < 1:1722916/68‑2 (MQ=255) gAATGCGTGCCCATTCCTGACGGGATGGGCATTTCTGCGCAACTTGTTGTCTTCTCAACAAATTACta < 1:121977/68‑2 (MQ=255) gAATGCGTGCCCATTCCTGACGGGATGGGCATTTCTGCGCAACTTGTTGTCTTCTCAACAAATTACta < 1:1339015/68‑2 (MQ=255) aaTGCGTGCCCATTCCTGACGGGATGGGCATTTCTGCGCAACTTGTTGTCTTCTCAACAAATTACta < 1:3057227/67‑2 (MQ=255) aTGCGTGCCCATTCCTGACGGAATGGGCATTTCTGCGCAACtt < 1:2481625/43‑1 (MQ=255) gTGCCCATTCCTGACGGGATGGGCATTTCTGCGCaa > 1:1347529/1‑36 (MQ=255) gTGCCCATTCCTGACGGGATGGGCATTTCTGCGCAACTTGTTGTCTTCTCAACAAATTACTACTTGctc > 1:1068442/1‑69 (MQ=255) gTGCCCATTCCTGACGGGATGGGCATTTCTGCGCAACTTGTTGTCTTCTCAACAAATTACTACTTGctc > 1:889225/1‑69 (MQ=255) gTGCCCATTCCTGACGGGATGGGCATTTCTGCGCAACTTGTTGTCTTCTCAACAAATTACTACTTGctc > 1:847072/1‑69 (MQ=255) gTGCCCATTCCTGACGGGATGGGCATTTCTGCGCAACTTGTTGTCTTCTCAACAAATTACTACTTGctc > 1:679305/1‑69 (MQ=255) gTGCCCATTCCTGACGGGATGGGCATTTCTGCGCAACTTGTTGTCTTCTCAACAAATTACTACTTGctc > 1:1533606/1‑69 (MQ=255) gTGCCCATTCCTGACGGGATGGGCATTTCTGCGCAACTTGTTGTCTTCTCAACAAATTACTACTTGctc > 1:154806/1‑69 (MQ=255) gTGCCCATTCCTGACGGGATGGGCATTTCTGCGCAACTTGTTGTCTTCTCAACAAATTACTACTTGctc > 1:1619587/1‑69 (MQ=255) gTGCCCATTCCTGACGGGATGGGCATTTCTGAGCAACTTGTTGTCTTCTCAACAAATTACTACTTGctc > 1:2575769/1‑69 (MQ=255) ccTGACGGAATGGGCATTTCTGCGCAACTTGTTGTCTTCTCAACAAATTACTGCTTGCTCTg > 1:674164/1‑62 (MQ=255) ccTGACGGAATGGGCATTTCTGCGCAACTTGTTGTCTTCTCAACAAATTACTGCTTGCTCTGGTCAGc > 1:828017/1‑68 (MQ=255) ccTGACGGAATGGGCATTTCTGCGCAACTTGTTGTCTTCTCAACAAATTACTGCTTGCTCTGGTCAGc > 1:1833577/1‑68 (MQ=255) ccTGACGGAATGGGCATTTCTGCGCAACTTGTTGTCTTCTCAACAAATTACTGCTTGCTCTGGTCAGc > 1:1895893/1‑68 (MQ=255) ccTGACGGAATGGGCATTTCTGCGCAACTTGTTGTCTTCTCAACAAATTACTGCTTGCTCTGGTCAGc > 1:2210956/1‑68 (MQ=255) cTGACGGAATGGGCATTTCTGCGCAACTTGTTGtctt < 1:1543716/37‑1 (MQ=255) | TCGAAAAACTCGCCTCTGCGGTAGATACCCGCTTCTGAATGCGTGCCCATTCCTGACGGAATGGGCATTTCTGCGCAACTTGTTGTCTTCTCAACAAATTACTGCTTGCTCTGGTCAGC > minE/1117135‑1117253 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |