| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,119,187 | G→A | 62.9% | H175H (CAC→CAT) | ydiV ← | conserved hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,119,187 | 0 | G | A | 62.9% | 31.8 / 37.8 | 62 | H175H (CAC→CAT) | ydiV | conserved hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base G (15/8); new base A (8/31); total (23/39) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 8.59e-04 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.98e-01 | |||||||||||
TGCAACAAGGGGAAATTTGCGCCTGGATCGCACGAATAAACGGTTCGAAGGAGCGGTGCGTGATTTGTTGCTGAATAAAACTTTTATCCAGCATGACGCGGGTGAAAAGAC > minE/1119132‑1119242 | tGCAACAAGGGGAAATTTGCGCCTGGATCGCACGAATAAACGGTTCGAAGGAGCGGTGCGTGATttgtt > 1:3289900/1‑69 (MQ=255) gCAACAAGGGGAAATTTGCGCCTGGATCGCACGAATAAACGGTTCGAAGGAGCGGTGCGTGATttgttg > 1:2143012/1‑69 (MQ=255) gCAACAAGGGGAAATTTGCGCCTGGATCGCACGAATAAACGGTTCGAAGGAGCGGTGCGTGATttgttg > 1:3382611/1‑69 (MQ=255) gCAACAAGGGGAAATTTGCGCCTGGATCGCACGAATAAACGGTTCGAAGGAGCGGTGCGTGATttgttg > 1:374708/1‑69 (MQ=255) gCAACAAGGGGAAATTTGCGCCTGGATCGCACGAATAAACGGTTCGAAGGAGCGGTGCGTGATttgttg > 1:2997404/1‑69 (MQ=255) gCAACAAGGGGAAATTTGCGCCTGGATCGCACGAATAAACGGTTCGAAGGAGCGGTGCGTGATttgttg > 1:2642883/1‑69 (MQ=255) gCAACAAGGGGAAATTTGCGCCTGGATCGCACGAATAAACGGTTCGAAGGAGCGGTGCGTGATttgttg > 1:2074094/1‑69 (MQ=255) gCAACAAGGGGAAATTTGCGCCTGGATCGCACGAATAAACGGTTCGAAGGAGCGGTGCGTGATttgttg > 1:1919407/1‑69 (MQ=255) gCAACAAGGGGAAATTTGCGCCTGGATCGCACGAATAAACGGTTCGAAGGAGCGGTGCGTGATttgttg > 1:1740563/1‑69 (MQ=255) gCAACAAGGGGAAATTTGCGCCTGGATCGCACGAATAAACGGTTCGAAGGAGCGGTGCGTGATttgttg > 1:919546/1‑69 (MQ=255) gCAACAAGGGGAAATTTGCGCCTGGATCGCACGAATAAACGGTTCGAAGGAGCGGTGCGTGATttgttg > 1:1188441/1‑69 (MQ=255) gAAATTTGCGCCTGGATCGCACGAATAAACGGTTCGAAGGAGCGATGCGTGAtt > 1:1569910/1‑54 (MQ=255) gAAATTTGCGCCTGGATCGCACGAATAAACGGTTCGAAGGAGCGATGCGTGATTTGTTGCTGAATaaaa > 1:2939738/1‑69 (MQ=255) gAAATTTGCGCCTGGATCGCACGAATAAACGGTTCGAAGGAGCGATGCGTGATTTGTTGCTGAATaaaa > 1:662953/1‑69 (MQ=255) gAAATTTGCGCCTGGATCGCACGAATAAACGGTTCGAAGGAGCGATGCGTGATTTGTTGCTGAATaaaa > 1:2171487/1‑69 (MQ=255) gAAATTTGCGCCTGGATCGCACGAATAAACGGTTCGAAGGAGCGATGCGTGATTTGTTGCTGAATaaaa > 1:1727404/1‑69 (MQ=255) gAAATTTGCGCCTGGATCGCACGAATAAACGGTTCGAAGGAGCGATGCGTGATTTGTTGCTGAATaaaa > 1:3084806/1‑69 (MQ=255) gAAATTTGCGCCTGGATCGCACGAATAAACGGTTCGAAGGAGCGATGCGTGATTTGTTGCTGAATaaaa > 1:1097490/1‑69 (MQ=255) gAAATTTGCGCCTGGATCGCACGAATAAACGGTTCGAAGGAGCGATGCGTGATTTGTTGCTGAATaaaa > 1:1093546/1‑69 (MQ=255) tGCGCCTGGATCTCACGAATAAACGGTTCGAAGGAGCGATGCGTg < 1:2080178/45‑1 (MQ=38) tGCGCCTGGATCGCACGAATAAACGGTTCGAAGGAGCGATGCGTg < 1:812302/45‑1 (MQ=255) tGCGCCTGGATCGCACGAATAAACGGTTCGAAGGAGCGATGCGTg < 1:477695/45‑1 (MQ=255) tGCGCCTGGATCGCACGAATAAACGGTTCGAAGGAGCGATGCGTg < 1:2828879/45‑1 (MQ=255) tGCGCCTGGATCGCACGAATAAACGGTTCGAAGGAGCGATGCGTg < 1:3294008/45‑1 (MQ=255) tGCGCCTGGATCGCACGAATAAACGGTTCGAAGGAGCGATGCGTg < 1:270232/45‑1 (MQ=255) tGCGCCTGGATCGCACGAATAAACGGTTCGAAGGAGCGATGCGTg < 1:2696953/45‑1 (MQ=255) tGCGCCTGGATCGCACGAATAAACGGTTCGAAGGAGCGATGCGTg < 1:1082939/45‑1 (MQ=255) tGCGCCTGGATCGCACGAATAAACGGTTCGAAGGAGCGATGCGTg < 1:2189240/45‑1 (MQ=255) tGCGCCTGGATCGCACGAATAAACGGTTCGAAGGAGCGATGCGTg < 1:1607468/45‑1 (MQ=255) tGCGCCTGGATCGCACGAATAAACGGTTCGAAGGAGCGATGCGTg < 1:1908084/45‑1 (MQ=255) tGCGCCTGGATCGCACGAATAAACGGTTCGAAGGAGCGATGCGTg < 1:2282588/45‑1 (MQ=255) tGCGCCTGGATCGCACGAATAAACGGTTCGAAGGAGCGATGCGTg < 1:131567/45‑1 (MQ=255) taTCGCACGAATAAACGGTTCGAAGGAGCGGTGCGTGATTTGTTGCTGAATAAAACTTTTATCCAGCAt > 1:1532431/2‑69 (MQ=255) gATCGCACGAATAAACGGTTCGAAGGAGCGGTTCGTGATTTGTTGCTGAATAAAACTTTTATCCAGCAt > 1:384838/1‑69 (MQ=255) gATCGCACGAATAAACGGTTCGAAGGAGCGGTGCGTGATTTGTTGCTGAATAAAACTTTTATCCAGCAt > 1:1712087/1‑69 (MQ=255) tCGCACGAATAAACGGTTCGAAGGAGCGGTGCGTGATTTGTTGCTGAATAAAACTTTTATCCAGCAt < 1:1755626/67‑1 (MQ=255) tCGCACGAATAAACGGTTCGAAGGAGCGGTGCGTGATTTGTTGCTGAATAAAACTTTTATCCAGCAt < 1:1380690/67‑1 (MQ=255) tCGCACGAATAAACGGTTCGAAGGAGCGGTGCGTGATTTGTTGCTGAATAAAACTTTTATCCAGCAt < 1:426154/67‑1 (MQ=255) tCGCACGAATAAACGGTTCGAAGGAGCGGTGCGTGATTTGTTGCTGAATAAAACTTTTATCCAGCAt < 1:3116244/67‑1 (MQ=255) tCGCACGAATAAACGGTTCGAAGGAGCGGTGCGTGATTTGTTGCTGAATAAAACTTTTATCCAGCAt < 1:3012559/67‑1 (MQ=255) tCGCACGAATAAACGGTTCGAAGGAGCGGTGCGTGATTTGTTGCTGAATAAAACTTTTATCCAGCAt < 1:825893/67‑1 (MQ=255) tCGCACGAATAAACGGTTCGAAGGAGCGGTGCGTGATTTGTTGCTGAATAAAACTTTTATCCAGCAt < 1:1282001/67‑1 (MQ=255) tCGCACGAATAAACGGTTCGAAGGAGCGGTGCGTGATTTGTTGCTGAATAAAACTTTTATCCAGCAt < 1:2462323/67‑1 (MQ=255) cGAATAAACGGTTCGAAGGAGCGGTGCGTGATTTGTTGCTGAATAAAACtttt > 1:656481/1‑53 (MQ=255) gatCGAAGGAGCGATGCGTGATTTGTTGCTGAATAAAACTTTTATCCAGCATGACGCGGGTGAAAAGAc < 1:1617911/67‑1 (MQ=255) gTTCGAAGGAGCGATGCGTGATTTGTTGCTGAATAAAACTTTTATCCAGCATGACGCGGGTGAAAAGAc < 1:502545/69‑1 (MQ=255) gTTCGAAGGAGCGATGCGTGATTTGTTGCTGAATAAAACTTTTATCCAGCATGACGCGGGTGAAAAGAc < 1:992747/69‑1 (MQ=255) gTTCGAAGGAGCGATGCGTGATTTGTTGCTGAATAAAACTTTTATCCAGCATGACGCGGGTGAAAAGAc < 1:1067090/69‑1 (MQ=255) gTTCGAAGGAGCGATGCGTGATTTGTTGCTGAATAAAACTTTTATCCAGCATGACGCGGGTGAAAAGAc < 1:876970/69‑1 (MQ=255) gTTCGAAGGAGCGATGCGTGATTTGTTGCTGAATAAAACTTTTATCCAGCATGACGCGGGTGAAAAGAc < 1:3410824/69‑1 (MQ=255) gTTCGAAGGAGCGATGCGTGATTTGTTGCTGAATAAAACTTTTATCCAGCATGACGCGGGTGAAAAGAc < 1:3381109/69‑1 (MQ=255) gTTCGAAGGAGCGATGCGTGATTTGTTGCTGAATAAAACTTTTATCCAGCATGACGCGGGTGAAAAGAc < 1:3357956/69‑1 (MQ=255) gTTCGAAGGAGCGATGCGTGATTTGTTGCTGAATAAAACTTTTATCCAGCATGACGCGGGTGAAAAGAc < 1:3339978/69‑1 (MQ=255) gTTCGAAGGAGCGATGCGTGATTTGTTGCTGAATAAAACTTTTATCCAGCATGACGCGGGTGAAAAGAc < 1:2904477/69‑1 (MQ=255) gTTCGAAGGAGCGATGCGTGATTTGTTGCTGAATAAAACTTTTATCCAGCATGACGCGGGTGAAAAGAc < 1:2841997/69‑1 (MQ=255) gTTCGAAGGAGCGATGCGTGATTTGTTGCTGAATAAAACTTTTATCCAGCATGACGCGGGTGAAAAGAc < 1:2666077/69‑1 (MQ=255) gTTCGAAGGAGCGATGCGTGATTTGTTGCTGAATAAAACTTTTATCCAGCATGACGCGGGTGAAAAGAc < 1:2665192/69‑1 (MQ=255) gTTCGAAGGAGCGATGCGTGATTTGTTGCTGAATAAAACTTTTATCCAGCATGACGCGGGTGAAAAGAc < 1:2550893/69‑1 (MQ=255) gTTCGAAGGAGCGATGCGTGATTTGTTGCTGAATAAAACTTTTATCCAGCATGACGCGGGTGAAAAGAc < 1:1780536/69‑1 (MQ=255) gTTCGAAGGAGCGATGCGTGATTTGTTGCTGAATAAAACTTTTATCCAGCATGACGCGGGTGAAAAGAc < 1:1331127/69‑1 (MQ=255) gTTCGAAGGAGCGATGCGTGATTTGTTGCTGAATAAAACTTTTATCCAGCATGACGCGGGTGAAAAGAc < 1:1315068/69‑1 (MQ=255) gTTCGAAGGAGCGATGCGTGATTTGTTGCTGAATAAAACTTTTATCCAGCATGACGCGGGTGAAAAGAc < 1:1210917/69‑1 (MQ=255) | TGCAACAAGGGGAAATTTGCGCCTGGATCGCACGAATAAACGGTTCGAAGGAGCGGTGCGTGATTTGTTGCTGAATAAAACTTTTATCCAGCATGACGCGGGTGAAAAGAC > minE/1119132‑1119242 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |