| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,121,639 | G→A | 47.7% | V54V (GTC→GTT) ‡ | btuE ← | predicted glutathione peroxidase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,121,639 | 0 | G | A | 47.7% | 8.4 / 54.4 | 65 | V54V (GTC→GTT) ‡ | btuE | predicted glutathione peroxidase |
| Reads supporting (aligned to +/- strand): ref base G (14/20); new base A (23/8); total (37/28) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.17e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.23e-01 | |||||||||||
GCTGCCCGGTTCTTGTTCCAGAAACTGGTTGCACGGGAATCCCAGCACCATAAAACCTCGATCGACCCAGGCTTTCTGAATATTCTCCAACTGCTCATATTGCGGCGTTAAGCCACACTTTGAGGCGACATT > minE/1121576‑1121707 | tcTGCCCGGTTCTTGTTCCAGAAACTGGTTGCACGGGAATCCCAGCACCATAAAACCTCGATCGACCCa > 1:2783808/2‑69 (MQ=255)gCTGCCCGGTTCTTGTTCCAGAAACTGGTTGCACGGGAATCCCAGCACCATAAAACCTCGATCGACCCa > 1:3428475/1‑69 (MQ=255)gCTGCCCGGTTCTTGTTCCAGAAACTGGTTGCACGGGAATCCCAGCACCATAAAACCTCGATCGACCCa > 1:2333210/1‑69 (MQ=255)gCTGCCCGGTTCTTGTTCCAGAAACTGGTTCCACGGGAATCCCAGCACCATAAAACCTCGATCGACCCa > 1:631572/1‑69 (MQ=255) ccAGAAACTGGTTGCACGGGAATCCCAGCACCATAAAACCTCGATCGACCCAGGCTTTCTGAATATtc > 1:2003058/1‑68 (MQ=255) ccAGAAACTGGTTGCACGGGAATCCCAGCACCATAAAACCTCGATCGACCCAGGCTTTCTGAATATtc > 1:899645/1‑68 (MQ=255) ccAGAAACTGGTTACACGGGAATCCCAGCACCACAAAACCACGATCAGCCCAGGCTTTCTGAATATtc < 1:3347877/68‑1 (MQ=255) ccAGAAACTGGTTACACGGGAATCCCAGCACCACAAAACCACGATCAGCCCAGGCTTTCTGAATATtc < 1:206436/68‑1 (MQ=255) ccAGAAACTGGTTACACGGGAATCCCAGCACCACAAAACCACGATCAGCCCAGGCTTTCTGAATATtc < 1:954514/68‑1 (MQ=255) ccAGAAACTGGTTACACGGGAATCCCAGCACCACAAAACCACGATCAGCCCAGGCTTTCTGAATATtc < 1:929009/68‑1 (MQ=255) ccAGAAACTGGTTACACGGGAATCCCAGCACCACAAAACCACGATCAGCCCAGGCTTTCTGAATATtc < 1:385883/68‑1 (MQ=255) ccAGAAACTGGTTACACGGGAATCCCAGCACCACAAAACCACGATCAGCCCAGGCTTTCTGAATATtc < 1:2272876/68‑1 (MQ=255) ccAGAAACTGGTTACACGGGAATCCCAGCACCACAAAACCACGATCAGCCCAGGCTTTCTGAATATtc < 1:2362070/68‑1 (MQ=255) ccAGAAACTGGTTACACGGGAATCCCAGCACCACAAAACCACGATCAGCCCAGGCTTTCTGAATATtc < 1:541073/68‑1 (MQ=255) ggTTGCACGGGAATCCCAGCACCATAAAACCTCGATCGACCCAGGCTTTCTGAATATTCTCCAACTGCt < 1:2891951/69‑1 (MQ=255) ggTTGCACGGGAATCCCAGCACCATAAAACCTCGATCGACCCAGGCTTTCTGAATATTCTCCAACTGCt < 1:2861827/69‑1 (MQ=255) ggTTGCACGGGAATCCCAGCACCATAAAACCTCGATCGACCCAGGATTTCTGAATATTCTCCAACTGCt < 1:2799681/69‑1 (MQ=255) tGCACGGGAATCCCAGCACCATAAAACCTCGATCGCCCCAGGCTTTCTGAATATTCTCCAACTGCTCa < 1:585735/68‑1 (MQ=255) tGCACGGGAATCCCAGCACCATAAAACCTCGATCGACCCAGGCTTTCTGAATATTCTCCAACTGCTCat < 1:1020093/69‑1 (MQ=255) tGCACGGGAATCCCAGCACCATAAAACCTCGATCGACCCAGGCTTTCTGAATATTCTCCAACTGCTCat < 1:547664/69‑1 (MQ=255) tGCACGGGAATCCCAGCACCATAAAACCTCGATCGACCCAGGCTTTCTGAATATTCTCCAACTGCTCa < 1:1902983/68‑1 (MQ=255) tGCACGGGAATCCCAGCACCATAAAACCTCGATCGACCCAGGCTTTCTGAATATTCTCCAACTGCTCa < 1:1924596/68‑1 (MQ=255) gCACGGGAATCCCAGCACCATAAAACCTCGATCGACCCAGGCTTTCTGAATATTCTCCAACTGCTCa < 1:1784506/67‑1 (MQ=255) gCACGGGAATCCCAGCACCATAAAACCTCGATCGACCCAGGCGTTCTGAATATTCTCCAACTGCTCa < 1:1014070/67‑1 (MQ=255) ggAATCCCAGCACCATAAAACCTCGATCGACCCAGGCTTTCTGAATATTCTCCAACTGCTCATATTgcg > 1:1051120/1‑69 (MQ=255) gAATCCCAGCACCACAAAACCACGATCAGCCCAGGc > 1:2183274/1‑36 (MQ=19) gAATCCCAGCACCACAAAACCACGATCAGCCCAGGCt > 1:2576707/1‑37 (MQ=21) gAATCCCAGCACCACAAAACCACGATCAGCCCAGGCt > 1:3395973/1‑37 (MQ=21) gAATCCCAGCACCACAAAACCACGATCAGCCCAGGCt > 1:3411250/1‑37 (MQ=21) gAATCCCAGCACCACAAAACCACGATCAGCCCAGGCt > 1:3274308/1‑37 (MQ=21) gAATCCCAGCACCACAAAACCACGATCAGCCCAGGCt > 1:3161735/1‑37 (MQ=21) gAATCCCAGCACCACAAAACCACGATCAGCCCAGGCt > 1:2980290/1‑37 (MQ=21) gAATCCCAGCACCACAAAACCACGATCAGCCCAGGCt > 1:2963872/1‑37 (MQ=21) gAATCCCAGCACCACAAAACCACGATCAGCCCAGGCt > 1:1130251/1‑37 (MQ=21) gAATCCCAGCACCACAAAACCACGATCAGCCCAGGCt > 1:1292487/1‑37 (MQ=21) gAATCCCAGCACCACAAAACCACGATCAGCCCAGGCt > 1:1296920/1‑37 (MQ=21) gAATCCCAGCACCACAAAACCACGATCAGCCCAGGCt > 1:1321929/1‑37 (MQ=21) gAATCCCAGCACCACAAAACCACGATCAGCCCAGGCt > 1:2707523/1‑37 (MQ=21) gAATCCCAGCACCACAAAACCACGATCAGCCCAGGCt > 1:1582942/1‑37 (MQ=21) gAATCCCAGCACCACAAAACCACGATCAGCCCAGGCt > 1:2022561/1‑37 (MQ=21) gAATCCCAGCACCACAAAACCACGATCAGCCCAGGCt > 1:171521/1‑37 (MQ=21) gAATCCCAGCACCACAAAACCACGATCAGCCCAGGCt > 1:469848/1‑37 (MQ=21) gAATCCCAGCACCACAAAACCACGATCAGCCCAGGCt > 1:630646/1‑37 (MQ=21) gAATCCCAGCACCACAAAACCACGATCAGCCCAGGCt > 1:630579/1‑37 (MQ=21) gAATCCCAGCACCACAAAACCACGATCAGCCCAGGCt > 1:2100877/1‑37 (MQ=21) gAATCCCAGCACCACAAAACCACGATCAGCCCAGGCt > 1:1835752/1‑37 (MQ=21) gAATCCCAGCACCACAAAACCACGATCAGCCCAGGCt > 1:1859232/1‑37 (MQ=21) gAATCCCAGCACCACAAAACCACGATCAGCCCAGGCt > 1:628020/1‑37 (MQ=21) ccAGCACCATAAAACCTCGATCGACCCAGGCTTTCTGaa < 1:2551721/39‑1 (MQ=255) ccAGCACCATAAAACCTCGATCGACCCAGGCTTTCTGaa < 1:2347281/39‑1 (MQ=255) ccAGCACCATAAAACCTCGATCGACCCAGGCTTTCTGaa < 1:1735397/39‑1 (MQ=255) ccAGCACCATAAAACCTCGATCGACCCAGGCTTTCTGAATATTCTcc > 1:432808/1‑47 (MQ=255) ccAGCACCATAAAACCTCGATCGACCCAGGCTTTCTGAATATTCTCCAACTGCTCata > 1:2162895/1‑58 (MQ=255) ccAGCACCATAAAACCTCGATCGACCCAGGCTTTCTGAATATTCTCCAACTGCTCATATTGCGGCGTTa > 1:979585/1‑69 (MQ=255) ccAGCACCATAAAACCTCGATCGACCCAGGCTTTCTGAATATTCTCCAACTGCTCATATTGCGGCGTTa > 1:2370488/1‑69 (MQ=255) ccAGCACCATAAAACCTCGATCGACCCAGGCTTTCTGAATATTCTCCAACTGCTCATATTGCGGCGTTa > 1:2322759/1‑69 (MQ=255) ccAGCACCATAAAACCTCGATCGACCCAGGCTTTCTGAATATTCTCCAACTGCTCATATTGCGGCGTTa > 1:1370958/1‑69 (MQ=255) ccAGCACCATAAAACCTCGATCGACCCAGGCTTTCTGAATATTCTCCAACTGCTCATATTGCGGCGTTa > 1:1759041/1‑69 (MQ=255) cATAAAACCTCGATCGACCCAGGCTTTCTGAATATTCTCCAACTGCTCATATTGCGGCGTTAAGCcaca < 1:2055692/69‑1 (MQ=255) cATAAAACCTCGATCGACCCAGGCTTTCTGAATATTCTCCAACTGCTCATATTGCGGCGTTAAGCcaca < 1:2035831/69‑1 (MQ=255) cATAAAACCTCGATCGACCCAGGCTTTCTGAATATTCTCCAACTGCTCATATTGCGGCGTTAAGCcaca < 1:1562107/69‑1 (MQ=255) aaaCCTCGATCGACCCAGGCTTTCTGAATATTCTCCAACTGCTCATATTGCGGCGTTAAGCca < 1:1014727/63‑1 (MQ=255) aaaCCTCGATCGACCCAGGCTTTCTGAATATTCTCCAACTGCTCATATTGCGGCGTTAAGCca < 1:1022195/63‑1 (MQ=255) aaaCCTCGATCGACCCAGGCTTTCTGAATATTCTCCAACTGCTCATATTGCGGCGTTAAGCca < 1:2598436/63‑1 (MQ=255) aaaCCTCGATCGACCCAGGCTTTCTGAATATTCTCCAACTGCTCATATTGCGGCGTTAAGCca < 1:1774602/63‑1 (MQ=255) cgaCCCAGGCTTTCTGAATATTCTCCAACTGCTCATATTGCGGCGTTAAGCCACACTTTGAGGCGACa > 1:3353886/1‑68 (MQ=255) cgaCCCAGGCTTTCTGAATATTCTCCAACTGCTCATATTGCGGCGTTAAGCCACACTTTGAGGCGACa > 1:784032/1‑68 (MQ=255) cgaCCCAGGCTTTCTGAATATTCTCCAACTGCTCATATTGCGGCGTTAAGCCACACTTTGAGGCGACa > 1:2310166/1‑68 (MQ=255) cgaCCCAGGCTTTCTGAATATTCTCCAACTGCTCATATTGCGGCGTTAAGCCACACTTTGAGGCGACa > 1:377734/1‑68 (MQ=255) cgaCCCAGGCTTTCTGAATATTCTCCAACTGCTCATATTGCGGCGATAAGCCACACt > 1:1629482/1‑57 (MQ=255) gaCCCAGGCTTTCTGAATATTCTCCAACTGCTCATATTGCGGCGTTAAGCCACACTTTGAGGCGACAtt < 1:1928885/69‑1 (MQ=255) gaCCCAGGCTTTCTGAATATTCTCCAACTGCTCATATTGCGGCGTTAAGCCACACTTTGAGGCGACAtt < 1:3372894/69‑1 (MQ=255) | GCTGCCCGGTTCTTGTTCCAGAAACTGGTTGCACGGGAATCCCAGCACCATAAAACCTCGATCGACCCAGGCTTTCTGAATATTCTCCAACTGCTCATATTGCGGCGTTAAGCCACACTTTGAGGCGACATT > minE/1121576‑1121707 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |