| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 93,879 | G→T | 100% | V287V (GTG→GTT) | secA → | preprotein translocase subunit, ATPase that targets protein precursors to the SecYE core translocon |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 93,879 | 0 | G | T | 75.7% | 89.7 / 35.2 | 70 | V287V (GTG→GTT) | secA | preprotein translocase subunit, ATPase that targets protein precursors to the SecYE core translocon |
| Reads supporting (aligned to +/- strand): ref base G (12/5); new base T (0/53); total (12/58) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.82e-10 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.64e-01 | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
GAAAAATCTCGCCAGGTGAACCTGACCGAACGTGGTCTGGTGCTGATTGAAGAACTGCTGGTGAAAGAGGGCATCATGGATGAAGGGGAGTCTCTGTACTCTCCGGCCAACATCATGCTGATGCACCACGT > minE/93817‑93947 | gAAAAATCTCGCCAGGTGAACCTGACCGAACGTGGTCTGGTGCTGATTGAAGAACTGCTGGTGAAagag < 1:287321/69‑1 (MQ=255)gAAAAATCTCGCCAGGTGAACCTGACCGAACGTGGTCTGGTGCTGATTGAAGAACTGCTGGTGAAagag < 1:2997656/69‑1 (MQ=255)gAAAAATCTCGCCAGGTGAACCTGACCGAACGTGGTCTGGTGCTGATTGAAGAACTGCTGGTGAAagag < 1:2018937/69‑1 (MQ=255)gAAAAATCTCGCCAGGTGAACCTGACCGAACGTGGTCTGGTGCTGATTGAAGAACTGCTGGTGAAagag < 1:2474609/69‑1 (MQ=255) cAGGTGAACCTGACCGATCGTGGTCTGGTTCTGATTGAAGAACTGCTGGTTAAAGAAGGcatcat < 1:1215017/65‑1 (MQ=255) cAGGTGAACCTGACCGAACGTGGTCTGGTTCTGATTGAAGAACTGCTTGTTAAAGAAGGcatcat < 1:2918473/65‑1 (MQ=255) cAGGTGAACCTGACCGAACGTGGTCTGGTTCTGATTGAAGAACTGCTGGTTAAAGAAGGcatcat < 1:524548/65‑1 (MQ=255) cAGGTGAACCTGACCGAACGTGGTCTGGTTCTGATTGAAGAACTGCTGGTTAAAGAAGGcatcat < 1:574536/65‑1 (MQ=255) cAGGTGAACCTGACCGAACGTGGTCTGGTTCTGATTGAAGAACTGCTGGTTAAAGAAGGcatcat < 1:2149046/65‑1 (MQ=255) cAGGTGAACCTGACCGAACGTGGTCTGGTTCTGATTGAAGAACTGCTGGTTAAAGAAGGcatcat < 1:512175/65‑1 (MQ=255) cAGGTGAACCTGACCGAACGTGGTCTGGTTCTGATTGAAGAACTGCTGGTTAAAGAAGGcatcat < 1:451758/65‑1 (MQ=255) cAGGTGAACCTGACCGAACGTGGTCTGGTTCTGATTGAAGAACTGCTGGTTAAAGAAGGcatcat < 1:3355088/65‑1 (MQ=255) cAGGTGAACCTGACCGAACGTGGTCTGGTTCTGATTGAAGAACTGCTGGTTAAAGAAGGcatcat < 1:2282170/65‑1 (MQ=255) cAGGTGAACCTGACCGAACGTGGTCTGGTTCTGATTGAAGAACTGCTGGTTAAAGAAGGcatcat < 1:2288574/65‑1 (MQ=255) cAGGTGAACCTGACCGAACGTGGTCTGGTTCTGATTGAAGAACTGCTGGTTAAAGAAGGcatcat < 1:3189119/65‑1 (MQ=255) cAGGTGAACCTGACCGAACGTGGTCTGGTTCTGATTGAAGAACTGCTGGTTAAAGAAGGcatcat < 1:3117254/65‑1 (MQ=255) cAGGTGAACCTGACCGAACGTGGTCTGGTTCTGATTGAAGAACTGCTGGTTAAAGAAGGcatcat < 1:2417649/65‑1 (MQ=255) cAGGTGAACCTGACCGAACGTGGTCTGGTTCTGATTGAAGAACTGCTGGTTAAAGAAGGcatcat < 1:3096329/65‑1 (MQ=255) cAGGTGAACCTGACCGAACGTGGTCTGGTTCTGATTGAAGAACTGCTGGTTAAAGAAGGcatcat < 1:3065536/65‑1 (MQ=255) cAGGTGAACCTGACCGAACGTGGTCTGGTTCTGATTGAAGAACTGCTGGTTAAAGAAGGcatcat < 1:2619060/65‑1 (MQ=255) cAGGTGAACCTGACCGAACGTGGTCTGGTTCTGATTGAAGAACTGCTGGTTAAAGAAGGcatcat < 1:2639122/65‑1 (MQ=255) cAGGTGAACCTGACCGAACGTGGTCTGGTTCTGATTGAAGAACTGCTGGTTAAAGAAGGcatcat < 1:2731262/65‑1 (MQ=255) cAGGTGAACCTGACCGAACGTGGTCTGGTTCTGATTGAAGAACTGCTGGTTAAAGAAGGcatcat < 1:1981038/65‑1 (MQ=255) cAGGTGAACCTGACCGAACGTGGTCTGGTTCTGATTGAAGAACTGCTGGTTAAAGAAGGcatcat < 1:1113964/65‑1 (MQ=255) cAGGTGAACCTGACCGAACGTGGTCTGGTTCTGATTGAAGAACTGCTGGTTAAAGAAGGcatcat < 1:1238656/65‑1 (MQ=255) cAGGTGAACCTGACCGAACGTGGTCTGGTTCTGATTGAAGAACTGCTGGTTAAAGAAGGcatcat < 1:878543/65‑1 (MQ=255) cAGGTGAACCTGACCGAACGTGGTCTGGTTCTGATTGAAGAACTGCTGGTTAAAGAAGGcatcat < 1:1468923/65‑1 (MQ=255) cAGGTGAACCTGACCGAACGTGGTCTGGTTCTGATTGAAGAACTGCTGGTTAAAGAAGGcatcat < 1:1473518/65‑1 (MQ=255) cAGGTGAACCTGACCGAACGTGGTCTGGTTCTGATTGAAGAACTGCTGGTTAAAGAAGGcatcat < 1:816505/65‑1 (MQ=255) cAGGTGAACCTGACCGAACGTGGTCTGGTTCTGATTGAAGAACTGCTGGTTAAAGAAGGcatcat < 1:1749396/65‑1 (MQ=255) cAGGTGAACCTGACCGAACGTGGTCTGGTTCTGATTGAAGAACTGCTGGTTAAAGAAGGcatcat < 1:712179/65‑1 (MQ=255) cAGGTGAACCTGACCGAACGTGGTCTGGTTCTGATTGAAGAACTGCTGGTTAAAGAAGGcatcat < 1:185307/65‑1 (MQ=255) tggTGAACCTGACCGAACGTGGTATGGTTCTGATTGAAGAACTCCTGGTTAAAGAAGGcatctt < 1:683698/63‑3 (MQ=255) aGGTGAACCTGACCGAACGTGGTCTGGTTCTGATTGAAGAACTGCTGGTTAAAGAAGGcatcat < 1:1719692/64‑1 (MQ=255) cGAACGTGGTCTGGTTCTGATTGAAGAACTGCTGGTTAAAGAAGGCATCATGGATGAAGGTGAGtct < 1:744762/67‑1 (MQ=255) cGAACGTGGTCTGGTTCTGATTGAAGAACTGCTGGTTAAAGAAGGCATCATGGATGAAGGTGAGtct < 1:780008/67‑1 (MQ=255) cGAACGTGGTCTGGTTCTGATTGAAGAACTGCTGGTTAAAGAAGGCATCATGGATGAAGGTGAGtct < 1:865838/67‑1 (MQ=255) cGAACGTGGTCTGGTTCTGATTGAAGAACTGCTGGTTAAAGAAGGCATCATGGATGAAGGTGAGtct < 1:739331/67‑1 (MQ=255) cGAACGTGGTCTGGTTCTGATTGAAGAACTGCTGGTTAAAGAAGGCATCATGGATGAAGGTGAGtct < 1:893668/67‑1 (MQ=255) cGAACGTGGTCTGGTTCTGATTGAAGAACTGCTGGTTAAAGAAGGCATCATGGATGAAGGTGAGtct < 1:2900949/67‑1 (MQ=255) cGAACGTGGTCTGGTTCTGATTGAAGAACTGCTGGTTAAAGAAGGCATCATGGATGAAGGTGAGtct < 1:2650475/67‑1 (MQ=255) cGAACGTGGTCTGGTTCTGATTGAAGAACTGCTGGTTAAAGAAGGCATCATGGATGAAGGTGAGtct < 1:918014/67‑1 (MQ=255) cGAACGTGGTCTGGTTCTGATTGAAGAACTGCTGGTTAAAGAAGGCATCATGGATGAAGGTGAGtct < 1:2864640/67‑1 (MQ=255) cGAACGTGGTCTGGTTCTGATTGAAGAACTGCTGGTTAAAGAAGGCATCATGGATGAAGGTGAGtct < 1:184957/67‑1 (MQ=255) cGAACGTGGTCTGGTTCTGATTGAAGAACTGCTGGTTAAAGAAGGCATCATGGATGAAGGTGAGtct < 1:2024265/67‑1 (MQ=255) cGAACGTGGTCTGGTTCTGATTGAAGAACTGCTGGTTAAAGAAGGCATCATGGATGAAGGTGAGtct < 1:1845062/67‑1 (MQ=255) cGAACGTGGTCTGGTTCTGATTGAAGAACTGCTGGTTAAAGAAGGCATCATGGATGAAGGTGAGtct < 1:2190375/67‑1 (MQ=255) cGAACGTGGTCTGGTTCTGATTGAAGAACTGCTGGTTAAAGAAGGCATCATGGATGAAGGTGAGtct < 1:2386611/67‑1 (MQ=255) cGAACGTGGTCTGGTTCTGATTGAAGAACTGCTGGTTAAAGAAGGCATCATGGATGAAGGTGAGtct < 1:2491609/67‑1 (MQ=255) cGAACGTGGTCTGGTTCTGATTGAAGAACTGCTGGTTAAAGAAGGCATCATGGATGAAGGTGAGtct < 1:2521436/67‑1 (MQ=255) cGAACGTGGTCTGGTTCTGATTGAAGAACTGCTGGTTAAAGAAGGCATCATGGATGAAGGTGAGtct < 1:1024700/67‑1 (MQ=255) cGAACGTGGTCTGGTTCTGATTGAAGAACTGCTGGTTAAAGAAGGCATCATGGATGAAGGTGAGtct < 1:2650835/67‑1 (MQ=255) cGAACGTGGTCTGGTTCTGATTGAAGAACTGCTGGTTAAAGAAGGCATCATGGATGAAGGTGAGtct < 1:2663221/67‑1 (MQ=255) cGAACGTGGTCTGGTTCTGATTGAAGAACTGCTGGTTAAAGAAGGCATCATGGATGAAGGGGAGtct < 1:440775/67‑1 (MQ=255) cGAACGTGGTCTGGTTCTGATTGAAGAACTGCTGGTTAAAGAAGGCATCATGGAAGAAGGTGAGtct < 1:1524197/67‑1 (MQ=255) gAACGTGGTCTGGTTCTGATTGAAGAACTGCTGGTTAAAGAAGGCATCATGGATGAAGGTGAGtct < 1:2314019/66‑1 (MQ=255) aCGTGGTCTGGTGCTGATTGAAGAACTGCTGGTGAAAGAGGGCATCATGGATGAAGGGGAGTCTCTGTa < 1:3435001/69‑1 (MQ=255) tCTGGTTCTGATTGAAGAACTGCTGGTTAAAGAAGGcatcat < 1:1315512/42‑1 (MQ=25) gaagaaCTGCTGGTGAAAGAGGGCATCATGGATGAAGGGGAGTCTCTg > 1:1541191/1‑48 (MQ=255) gaagaaCTGCTGGTGAAAGAGGGCATCATGGATGAAGGGGAGTCTCTg > 1:3294195/1‑48 (MQ=255) gaagaaCTGCTGGTGAAAGAGGGCATCATGGATGAAGGGGAGTCTCTg > 1:1813661/1‑48 (MQ=255) gaagaaCTGCTGGTGAAAGAGGGCATCATGGATGAAGGGGAGTCTCTg > 1:615093/1‑48 (MQ=255) aCTGCTGGTGAAAGAGGGCATCATGGATGAAGGGGAGTCTCTGTAct > 1:2928701/1‑47 (MQ=255) aCTGCTGGTGAAAGAGGGCATCATGGATGAAGGGGAGTCTCTGTACTCTCCGGCCAACATCATGCTGa > 1:1697614/1‑68 (MQ=255) aCTGCTGGTGAAAGAGGGCATCATGGATGAAGGGGAGTCTCTGTACTCTCCGGCCAACATCATGCTGa > 1:2827515/1‑68 (MQ=255) aCTGCTGGTGAAAGAGGGCATCATGGATGAAGGGGAGTCTCTGTACTCTCCGGCCAACATCATGCTGa > 1:2127653/1‑68 (MQ=255) aCTGCTGGTGAAAGAGGGCATCATGGATGAAGGGGAGTCTCTGTACTCTCCGGCCAACATCATGCTGa > 1:2259276/1‑68 (MQ=255) aCTGCTGGTGAAAGAGGGCATCATGGATGAAGGGGAGTCTCTGTACTCTCCGGCCAACATCATGCTGa > 1:3268904/1‑68 (MQ=255) ctgctgGTGAAAGAGGGCATCATGGATGAAGGGGAGTCTCTGTACTCTCCGGCCAACATCATGCTGATg > 1:1565062/1‑69 (MQ=255) ctgctgGTGAAAGAGGGCATCATGGATGAAGGGGAGTCTCTGTACTCTCCGG‑CAACATCATGCTGATGc > 1:2235099/1‑69 (MQ=255) gAAAGAGGGCATCATGGATGAAGGGGAGTCTCTGTACTCTCCGGCCAACATCATGCTGATGCACCACGt < 1:3260530/69‑1 (MQ=255) gAAAGAGGGCATCATGGATGAAGGGGAGTCTCTGTACTCTCCGGCCAACATCATGCTGATGCACCACGt < 1:3206571/69‑1 (MQ=255) gAAAGAGGGCATCATGGATGAAGGGGAGTCTCTGTACTCTCCGGCCAACATCATGCTGATGCACCACGt < 1:1189179/69‑1 (MQ=255) gAAAGAGGGCATCATGGATGAAGGGGAGTCTCTGTACTCTCCGGCCAACATCATGCTGATGCACCACGt < 1:1052941/69‑1 (MQ=255) | GAAAAATCTCGCCAGGTGAACCTGACCGAACGTGGTCTGGTGCTGATTGAAGAACTGCTGGTGAAAGAGGGCATCATGGATGAAGGGGAGTCTCTGTACTCTCCGGCCAACATCATGCTGATGCACCACGT > minE/93817‑93947 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |