| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,125,105 | A→G | 54.8% | R177R (CGT→CGC) | pheT ← | phenylalanine tRNA synthetase, beta subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,125,105 | 0 | A | G | 54.8% | 17.4 / 66.8 | 84 | R177R (CGT→CGC) | pheT | phenylalanine tRNA synthetase, beta subunit |
| Reads supporting (aligned to +/- strand): ref base A (21/17); new base G (28/18); total (49/35) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.60e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.12e-01 | |||||||||||
GGTCGCACCAACCGGAACGATTTCCGGTTGAACCAGCGGCAGCTGGTTCAGCACGGCAACGTCACGCGCAACACCAATGATGCCTAAGCAGTCGGCACGGTTTGGCGTCACGCTGATTTCGATG > minE/1125042‑1125165 | ggTCGCACCAACCGGAACGATTTCCGGTTGAACCAGCGGCAGCTGGTTCAGCACGGCAACGTCACgcgc > 1:2180302/1‑69 (MQ=255)ggTCGCACCAACCGGAACGATTTCCGGTTGAACCAGCGGCAGCTGGTTCAGCACGGCAACGTCACgcgc > 1:2172309/1‑69 (MQ=255)ggTCGCACCAACCGGAACGATTTCCGGTTGAACCAGCGGCAGCTGGTTCAGCACGGCAACGTCACgcgc > 1:799620/1‑69 (MQ=255)ggTCGCACCAACCGGAACGATTTCCGGTTGAACCAGCGGCAGCTGGTTCAGCACGGCAACGTCACgcgc > 1:723087/1‑69 (MQ=255)ggTCGCACCAACCGGAACGATTTCCGGTTGAACCAGCGGCAGCTGGTTCAGCACGGCAACGTCACgcgc > 1:2210105/1‑69 (MQ=255)ggTCGCACCAACCGGAACGATTTCCGGTTGAACCAGCGGCAGCTGGTTCAGCACGGCAACGTCACgcgc > 1:2798365/1‑69 (MQ=255)ggTCGCACCAACCGGAACGATTTCCGGTTGAACCAGCGGCAGCTGGTTCAGCACGGCAACGTCACgcgc > 1:3286779/1‑69 (MQ=255)ggTCGCACCAACCGGAACGATTTCCGGTTGAACCAGCGGCAGCTGGTTCAGCACGGCAACGTCACgcgc > 1:2807485/1‑69 (MQ=255) tCGCACCAACCGGAACGATTTCCGGTTGAACCAGCGGCAGCTGGTTCAGCACGGCAACGTCACGCGCaa > 1:2253249/1‑69 (MQ=255) aCCAACCGGAACGATTTCCGGTTCAACCAGCGGCAGCTGGTTGAACACGGCAACGTCGCGCGCAACAcc > 1:3241539/1‑69 (MQ=255) aCCAACCGGAACGATTTCCGGTTCAACCAGCGGCAGCTGGTTCAACACGGCAACGTCGCGCGCAacac > 1:2187984/1‑68 (MQ=255) aCCAACCGGAACGATTTCCGGTTCAACCAGCGGCAGCTGGTTCAACACGGCAACGTCGCGCGCAACAcc > 1:88472/1‑69 (MQ=255) aCCAACCGGAACGATTTCCGGTTCAACCAGCGGCAGCTGGTTCAACACGGCAACGTCGCGCGCAACAcc > 1:2167796/1‑69 (MQ=255) aCCAACCGGAACGATTTCCGGTTCAACCAGCGGCAGCTGGTTCAACACGGCAACGTCGCGCGCAACAcc > 1:2934512/1‑69 (MQ=255) aCCAACCGGAACGATTTCCGGTTCAACCAGCGGCAGCTGGTTCAACACGGCAACGTCGCGCGCAACAcc > 1:1812798/1‑69 (MQ=255) aCCAACCGGAACGATTTCCGGTTCAACCAGCGGCAGCTGGTTCAACACGGCAACGTCGCGCGCAACAcc > 1:3357169/1‑69 (MQ=255) aCCAACCGGAACGATTTCCGGTTCAACCAGCGGCAGCTGGTTCAACACGGCAACGTCGCGCGCAACAcc > 1:2798925/1‑69 (MQ=255) aCCAACCGGAACGATTTCCGGTTCAACCAGCGGCAGCTGGTTCAACACGGCAACGTCGCGCGCAACAcc > 1:1659409/1‑69 (MQ=255) aCCAACCGGAACGATTTCCGGTTCAACCAGCGGCAGCTGGTTCAACACGGCAACGTCGCGCGCAACAcc > 1:2327500/1‑69 (MQ=255) aCCAACCGGAACGATTTCCGGTTCAACCAGCGGCAGCTGGTTCAACACGGCAACGTCGCGCGCAACAcc > 1:1404104/1‑69 (MQ=255) aCCAACCGGAACGATTTCCGGTTCAACCAGCGGCAGCTGGTTCAACACGGCAACGTCGCGCGCAACAcc > 1:616876/1‑69 (MQ=255) aCCAACCGGAACGATTTCCGGTTCAACCAGCGGCAGCTGGTTCAACACGGCAACGTCGCGCGCAACAcc > 1:1197273/1‑69 (MQ=255) agccGGAACGATTTCCGGTTGAACCAGCGGCAGCTGGTTCAGCACGGCAACGTCACGCGCAACACCAat < 1:1884758/67‑1 (MQ=255) aaCCGGAACGATTTCCTGTTGAACCAGCGGCAGCTGGTTCAGCACGGCAACGTCACGCGCAACACCAat < 1:2819441/69‑1 (MQ=255) aaCCGGAACGATTTCCGGTTTAACCAGCGGCAGCTGGTTCAGCACGGCAACGTCACGCGCAACACCAat < 1:2180433/69‑1 (MQ=255) aaCCGGAACGATTTCCGGTTGAACCAGCGGCAGCTGGTTCAGCACGGCAACGTCACGCGCAACACCAat < 1:2817411/69‑1 (MQ=255) aaCCGGAACGATTTCCGGTTGAACCAGCGGCAGCTGGTTCAGCACGGCAACGTCACGCGCAACACCAat < 1:2331151/69‑1 (MQ=255) aaCCGGAACGATTTCCGGTTGAACCAGCGGCAGCTGGTTCAGCACGGCAACGTCACGCGCAACACCAat < 1:2730926/69‑1 (MQ=255) aaCCGGAACGATTTCCGGTTGAACCAGCGGCAGCTGGTTCAGCACGGCAACGTCACGCGCAACACCAat < 1:300631/69‑1 (MQ=255) aaCCGGAACGATTTCCGGTTGAACCAGCGGCAGCTGGTTCAGCACGGCAACGTCACGCGCAACACCAat < 1:3016695/69‑1 (MQ=255) aaCCGGAACGATTTCCGGTTGAACCAGCGGCAGCTGGTTCAGCACGGCAACGTCACGCGCAACACCAat < 1:305769/69‑1 (MQ=255) aaCCGGAACGATTTCCGGTTGAACCAGCGGCAGCTGGTTCAGCACGGCAACGTCACGCGCAACACCAat < 1:196456/69‑1 (MQ=255) aaCCGGAACGATTTCCGGTTGAACCAGCGGCAGCTGGTTCAGCACGGCAACGTCACGCGCAACACCAat < 1:3298333/69‑1 (MQ=255) aaCCGGAACGATTTCCGGTTGAACCAGCGGCAGCTGGTTCAGCACGGCAACGTCACGCGCAACACCAat < 1:337432/69‑1 (MQ=255) aaCCGGAACGATTTCCGGTTGAACCAGCGGCAGCTGGTTCAGCACGGCAACGTCACGCGCAACACCAat < 1:393408/69‑1 (MQ=255) aaCCGGAACGATTTCCGGTTGAACCAGCGGCAGCTGGTTCAGCACGGCAACGTCACGCGCAACACCAat < 1:1483432/69‑1 (MQ=255) aaCCGGAACGATTTCCGGTTGAACCAGCGGCAGCTGGTTCAGCACGGCAACGTCACGCGCAACACCAat < 1:1156618/69‑1 (MQ=255) aaCCGGAACGATTTCCGGTTGAACCAGCGGCAGCTGGTTCAGCACGGCAACGGCACGCGCAACACCAat < 1:1127110/69‑1 (MQ=255) tGAACCAGCGGCAGCTGGTTCAGCACGGCAACGTCACGCGCAACACCAat < 1:1764894/50‑1 (MQ=255) tGAACCAGCGGCAGCTGGTTCAGCACGGCAACGTCACGCGCAACACCAATGATGCCTAAGCAGTCGGCa > 1:22615/1‑69 (MQ=255) tGAACCAGCGGCAGCTGGTTCAGCACGGCAACGTCACGCGCAACACCAATGATGCCTAAGCAGTCGGCa > 1:2019938/1‑69 (MQ=255) ggCAGCTGGTTCAACACGGCAACGTCGCGCGCAACACCAATGATACCTAAGCAGTCGGCACGGTTTGGc < 1:1110785/69‑1 (MQ=255) ggCAGCTGGTTCAACACGGCAACGTCGCGCGCAACACCAATGATACCTAAGCAGTCGGCACGGTTTGGc < 1:2530924/69‑1 (MQ=255) ggCAGCTGGTTCAACACGGCAACGTCGCGCGCAACACCAATGATACCTAAGCAGTCGGCACGGTTTGGc < 1:728402/69‑1 (MQ=255) ggCAGCTGGTTCAACACGGCAACGTCGCGCGCAACACCAATGATACCTAAGCAGTCGGCACGGTTTGGc < 1:1293628/69‑1 (MQ=255) ggCAGCTGGTTCAACACGGCAACGTCGCGCGCAACACCAATGATACCTAAGCAGTCGGCACGGTTTGGc < 1:3380884/69‑1 (MQ=255) ggCAGCTGGTTCAACACGGCAACGTCGCGCGCAACACCAATGATACCTAAGCAGTCGGCACGGTTTGGc < 1:3262722/69‑1 (MQ=255) ggCAGCTGGTTCAACACGGCAACGTCGCGCGCAACACCAATGATACCTAAGCAGTCGGCACGGTTTGGc < 1:2133777/69‑1 (MQ=255) ggCAGCTGGTTCAACACGGCAACGTCGCGCGCAACACCAATGATACCTAAGCAGTCGGCACGGTTTGGc < 1:3137979/69‑1 (MQ=255) ggCAGCTGGTTCAACACGGCAACGTCGCGCGCAACACCAATGATACCTAAGCAGTCGGCACGGTTTGGc < 1:3103916/69‑1 (MQ=255) ggCAGCTGGTTCAACACGGCAACGTCGCGCGCAACACCAATGATACCTAAGCAGTCGGCACGGTTTGGc < 1:2115104/69‑1 (MQ=255) gCAGCTGGTTCAACACGGCAACGTCGCGCGCAACACCAATGATACCTAAGCAGTCGGCACGGTTTGGc < 1:2575739/68‑1 (MQ=255) cAGCACGGCAACGTCACGCGCAACACCAATGATGCCTAAGCAGTCGGCACGGTTTGGCGTCACGCTGa > 1:509062/1‑68 (MQ=255) cAGCACGGCAACGTCACGCGCAACACCAATGATGCCTAAGCAGTCGGCACGGTTTGGCGTCACGCTGAt > 1:605953/1‑69 (MQ=255) cAGCACGGCAACGTCACGCGCAACACCAATGATGCCTAAGCAGTCGGCACGGTTTGGCGTCACGCTGAt > 1:801611/1‑69 (MQ=255) cAGCACGGCAACGTCACGCGCAACACCAATGATGCCTAAGCAGTCGGCACGGTTTGGCGTCACGCTGAt > 1:2827767/1‑69 (MQ=255) cAGCACGGCAACGTCACGCGCAACACCAATGATGCCTAAGCAGTCGGCACGGTTTGGCGTCACGCTGAt > 1:475504/1‑69 (MQ=255) cAGCACGGCAACGTCACGCGCAACACCAATGATGCCTAAGCAGTCGGCACGGTTTGGCGTCACGCTGAt > 1:1347890/1‑69 (MQ=255) cAGCACGGCAACGTCACGCGCAACACCAATGATGCCTAAGCAGTCGGCACGGTTTGGCGTCACGCTGAt > 1:3354816/1‑69 (MQ=255) cAGCACGGCAACGTCACGCGCAACACCAATGATGCCTAAGCAGTCGGCACGGTTTGGCGTCACGCTGAt > 1:2302479/1‑69 (MQ=255) cAGCACGGCAACGTCACGCGCAACACCAATGATGCCTAAGCAGTCGGCACGGTTTGGCGTCACGCTGAt > 1:1758570/1‑69 (MQ=255) aacACGGCAACGTCGCGCGCAACACCAATGATACCTGAGCAGTCGGCACTGTTTGGCGTCACGCTAAtt > 1:3366780/3‑69 (MQ=255) aacACGGCAACGTCGCGCGCAACACCAATGATACCTAAGCAGTCGGCACTGTTTGGCGTCACGCTAAtt > 1:226630/3‑69 (MQ=255) aacACGGCAACGTCGCGCGCAACACCAATGATACCTAAGCAGTCGGCACTGTTTGGCGTCACGCTAAtt > 1:421248/3‑69 (MQ=255) aacACGGCAACGTCGCGCGCAACACCAATGATACCTAAGCAGTCGGCACTGTTTGGCGTCACGCTAAtt > 1:1555673/3‑69 (MQ=255) aacACGGCAACGTCGCGCGCAACACCAATGATACCTAAGCAGTCGGCACTGTTTGGCGTCACGCTAAtt > 1:613496/3‑69 (MQ=255) aacACGGCAACGTCGCGCGCAACACCAATGATACCTAAGCAGTCGGCACTGTTTGGCGTCACGCTAAtt > 1:3367547/3‑69 (MQ=255) aacACGGCAACGTCGCGCGCAACACCAATGATACCTAAGCAGTCGGCACTGTTTGGCGTCACGCTAAtt > 1:330599/3‑69 (MQ=255) aacACGGCAACGTCGCGCGCAACACCAATGATACCTAAGCAGTCGGCACTGTTTGGCGTCACGCTAAtt > 1:1846718/3‑69 (MQ=255) aacACGGCAACGTCGCGCGCAACACCAATGATACCTAAGCAGTCGGCACTGTTTGGCGTCACGCTAAtt > 1:1891516/3‑69 (MQ=255) aacACGGCAACGTCGCGCGCAACACCAATGATACCTAAGCAGTCGGCACTGTTTGGCGTCACGCTAAtt > 1:697509/3‑69 (MQ=255) aacACGGCAACGTCGCGCGCAACACCAATGATACCTAAGCAGTCGGCACTGTTTGGCGTCACGCTAAtt > 1:118739/3‑69 (MQ=255) aacACGGCAACGTCGCGCGCAACACCAATGATACCTAAGCAGTCGGCACTGTTTGGCGTCACGCTAAtt > 1:2909791/3‑69 (MQ=255) aacACGGCAACGTCGCGCGCAACACCAATGATACCTAAGCAGTCGGCACTGTTTGGCGTCACGCTAAtt > 1:19766/3‑69 (MQ=255) aacACGGCAACGTCGCGCGCAACACCAATGATACCTAAGCAGTCGGCACTGTTTGGCGTCACGCTAAt > 1:2554910/3‑68 (MQ=255) aacACGGCAACGTCGCGCGCAACACCAATGATACCTAAGCAGTCGGCACTGTTTGGCGTCACGCTAAt > 1:3420264/3‑68 (MQ=255) acACGGCAACGTCGCGCGCAACACCAATGATACCTAAGCAGTCGGCACGGTTTGGCGTCACGCTAAtt < 1:356624/67‑1 (MQ=255) acACGGCAACGTCGCGCGCAACACCAATGATACCTAAGCAGTCGGCACGGTTTGGCGTCACGCTAAtt < 1:2760630/67‑1 (MQ=255) acACGGCAACGTCGCGCGCAACACCAATGATACCTAAGCAGTCGGCACGGTTTGGCGTCACGCTAAtt < 1:1937142/67‑1 (MQ=255) acACGGCAACGTCGCGCGCAACACCAATGATACCTAAGCAGTCGGCACGGTTTGGCGTCACGCTAAtt < 1:3067396/67‑1 (MQ=255) acACGGCAACGTCGCGCGCAACACCAATGATACCTAAGCAGTCGGCACGGTTTGGCGTCACGCTAAtt < 1:806047/67‑1 (MQ=255) acACGGCAACGTCGCGCGCAACACCAATGATACCTAAGCAGTCGGCACGGTTTGGCGTCACGCTAAtt < 1:2073350/67‑1 (MQ=255) aCGGCAACGTCGCGCGCAACACCAATGATACCTAAGCAGTCGGCACGGTTTGGc < 1:2200353/54‑1 (MQ=255) gCAACGTCACGCGCAACACCAATGATGCCTAAGCAGTCGGCACGGTTTGGCGTCACGCTGATTTCGATg > 1:2777923/1‑69 (MQ=255) | GGTCGCACCAACCGGAACGATTTCCGGTTGAACCAGCGGCAGCTGGTTCAGCACGGCAACGTCACGCGCAACACCAATGATGCCTAAGCAGTCGGCACGGTTTGGCGTCACGCTGATTTCGATG > minE/1125042‑1125165 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |