Predicted mutation
evidence seq id position mutation freq annotation gene description
RA minE 1,125,241 C→T 42.8% S132N (AGC→AAC)  pheT ← phenylalanine tRNA synthetase, beta subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE1,125,2410CT42.8% 14.4 / 33.9 49S132N (AGC→AAC) pheTphenylalanine tRNA synthetase, beta subunit
Reads supporting (aligned to +/- strand):  ref base C (19/9);  new base T (9/12);  total (28/21)
Fisher's exact test for biased strand distribution p-value = 9.19e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.23e-01

AAGTTTCAGGTATTCACGGATATCGGTGCCAATCGGCGCATCCGCAGGCAGTTCGATAATGCCGCTGTGATCGTCAGAAATGCCCAGTTCAGAGAAGGAGCACAGCATCCCTTCAGAC  >  minE/1125177‑1125294
                                                                |                                                     
aaGTTTCAGGTGTTCACGGATATCGGTGCCAATCGGCGCATCCGCAGGCAGTTCGATAATGCCGCtgtg                                                   >  1:415096/1‑69 (MQ=255)
aaGTTTCAGGTATTCACGGATATCGGTGCCAATCGGCGCATCCGCAGGCAGTTCGATAATGCCGCtgtg                                                   >  1:3278753/1‑69 (MQ=255)
aaGTTTCAGGTATTCACGGATATCGGTGCCAATCGGCGCATCCGCAGGCAGTTCGATAATGCCGCtgt                                                    >  1:116204/1‑68 (MQ=255)
              cACGGATATCGGTGCCAATCGGCGCATCCGCAGGCAGTTCGATAATGCCGCTGTGATCGTCAGAAATg                                      >  1:564620/1‑68 (MQ=255)
              cACGGATATCGGTGCCAATCGGCGCATCCGCAGGCAGTTCGATAATGCCGCTGTGATCGTCAGAAATg                                      >  1:1652933/1‑68 (MQ=255)
                cGGATATCGGTGCCAATCGGCGCATCCGCAGGCAGTTCGATAATGCCGCTGTGATCTTCAGAAATGcc                                    <  1:3323942/68‑1 (MQ=255)
                cGGATATCGGTGCCAATCGGCGCATCCGCAGGCAGTTCGATAATGCCGCTGTGATCGTCAGAAATGcc                                    <  1:3414831/68‑1 (MQ=255)
                cGGATATCGGTGCCAATCGGCGCATCCGCAGGCAGTTCGATAATGCCGCTGTGATCGTCAGAAATGcc                                    <  1:3361924/68‑1 (MQ=255)
                cGGATATCGGTGCCAATCGGCGCATCCGCAGGCAGTTCGATAATGCCGCTGTGATCGTCAGAAATGcc                                    <  1:1724095/68‑1 (MQ=255)
                cGGATATCGGTGCCAATCGGCGCATCCGCAGGCAGTTCGATAATGCCGCTGTGATCGTCAGAAATGcc                                    <  1:1725806/68‑1 (MQ=255)
                cGGATATCGGTGCCAATCGGCGCATCCGCAGGCAGTTCGATAATGCCGCTGTGATCGTCAGAAATGcc                                    <  1:3164256/68‑1 (MQ=255)
                cGGATATCGGTGCCAATCGGCGCATCCGCAGGCAGTTCGATAATGCCGCTGTGATCGTCAGAAATGcc                                    <  1:2755185/68‑1 (MQ=255)
                cGGATATCGGTGCCAATCGGCGCATCCGCAGGCAGTTCGATAATGCCGCTGTGATCGTCAGAAATGcc                                    <  1:2530374/68‑1 (MQ=255)
                      tCGGTGCCAATCGGCGCATCCGCAGGCAGTTCGATAATGCCGCTGTGATCGTCAGAAATGCCCAGtt                               >  1:694876/1‑67 (MQ=255)
                      tCGGTGCCAATCGGCGCATCCGCAGGCAGTTCGATAATGCCGCTGTGATCGTCAGAAATGCCCAGtt                               >  1:3311181/1‑67 (MQ=255)
                      tCGGTGCCAATCGGCGCATCCGCAGGCAGTTCGATAATGCCGCTGTGATCGTCAGAAATGCCCAGtt                               >  1:2162765/1‑67 (MQ=255)
                                 cGGCGCATCCGCAGGCAGTTCGATAATGCCGCTGTGATCGTCAGAAATGCCCAGTTCAGAGAAGGAg                    >  1:1076854/1‑67 (MQ=255)
                                 cGGCGCATCCGCAGGCAGTTCGATAATGCCGCTGTGATCGTCAGAAATGCCCAGTTCAGAGAAGGAg                    >  1:2094388/1‑67 (MQ=255)
                                 cGGCGCATCCGCAGGCAGTTCGATAATGCCGCTGTGATCGTCAGAAATGCCCAGTTCAGAGAAGGAg                    >  1:1323974/1‑67 (MQ=255)
                                 cGGCGCATCCGCAGGCAGTTCGATAATGCCGCTGTGATCGTCAGAAATGCCCAGTTCAGAGAAGGAg                    >  1:30896/1‑67 (MQ=255)
                                 cGGCGCATCCGCAGGCAGTTCGATAATGCCGCTGTGATCGTCAGAAATGCCCAGTTCAGAGAAGGAg                    >  1:2731303/1‑67 (MQ=255)
                                 cGGCGCATCCGCAGGCAGTTCGATAATGCCGCTGTGATCGTCAGAAATGCCCAGTTCAGAGAAGGAg                    >  1:2015503/1‑67 (MQ=255)
                                 cGGCGCATCCGCAGGCAGTTCGATAATGCCGCTGTGATCGTCAGAAATGCCCAGTTCAGAGAAGGAg                    >  1:298326/1‑67 (MQ=255)
                                  ggCGCATCCGCAGGCAGTTCGATAATGCCGCTGTGATCGTCAGAAATGCCCAGTTCAGAGAAGGAGcac                 >  1:428574/1‑69 (MQ=255)
                                         ccGCAGGCAGCTCGATAATGCCGTTATGATCGTCAGa                                          >  1:2432646/1‑37 (MQ=25)
                                         ccGCAGGCAGCTCGATAATGCCGTTATGATCGTCAGAAATTCCCAGCTCagag                          >  1:3306439/1‑53 (MQ=25)
                                         ccGCAGGCAGCTCGATAATGCCGTTATGATCGTCAGAAATTCCCAGCTCagag                          >  1:3264922/1‑53 (MQ=25)
                                         ccGCAGGCAGCTCGATAATGCCGTTATGATCGTCAGAAATTCCCAGCTCagag                          >  1:3341988/1‑53 (MQ=25)
                                         ccGCAGGCAGCTCGATAATGCCGTTATGATCGTCAGAAATTCCCAGCTCagag                          >  1:3088946/1‑53 (MQ=25)
                                         ccGCAGGCAGCTCGATAATGCCGTTATGATCGTCAGAAATTCCCAGCTCagag                          >  1:1498079/1‑53 (MQ=25)
                                         ccGCAGGCAGCTCGATAATGCCGTTATGATCGTCAGAAATTCCCAGCTCagag                          >  1:2916455/1‑53 (MQ=25)
                                         ccGCAGGCAGCTCGATAATGCCGTTATGATCGTCAGAAATTCCCAGCTCagag                          >  1:537066/1‑53 (MQ=25)
                                         ccGCAGGCAGCTCGATAATGCCGTTATGATCGTCAGAAATTCCCAGCTCagag                          >  1:2477322/1‑53 (MQ=25)
                                          cGCAGGCAGTTCGATAATGCCGCTGTGATCGTCAGAAATGcc                                    <  1:2600140/42‑1 (MQ=255)
                                          cGCAGGCAGTTCGATAATGCCGCTGTGATCGTCAGAAATGCCCAGTTCAGAGAAGGAGCACAGCATccc         >  1:1717746/1‑69 (MQ=255)
                                          cGCAGGCAGTTCGATAATGCCGCTGTGATCGTCAGAAATGCCCAGTTCAGAGAAGGAGCACAGCATccc         >  1:1722096/1‑69 (MQ=255)
                                          cGCAGGCAGTTCGATAATGCCGCTGTGATCGTCAGAAATGCCCAGTTCAGAGAAGGAGCACAGCATccc         >  1:233506/1‑69 (MQ=255)
                                                 agctCGATAATGCCGTTATGATCGTCAGAAATTCCCAGCTCAGAGAAGGAGCACAGCATCCCTTCAGAc  <  1:173811/66‑1 (MQ=255)
                                                 agctCGATAATGCCGTTATGATCGTCAGAAATTCCCAGCTCAGAGAAGGAGCACAGCATCCCTTCAGAc  <  1:3180252/66‑1 (MQ=255)
                                                 agctCGATAATGCCGTTATGATCGTCAGAAATTCCCAGCTCAGAGAAGGAGCACAGCATCCCTTCAGAc  <  1:1762375/66‑1 (MQ=255)
                                                 agctCGATAATGCCGTTATGATCGTCAGAAATTCCCAGCTCAGAGAAGGAGCACAGCATCCCTTCAGAc  <  1:3073148/66‑1 (MQ=255)
                                                 agctCGATAATGCCGTTATGATCGTCAGAAATTCCCAGCTCAGAGAAGGAGCACAGCATCCCTTCAGAc  <  1:1484348/66‑1 (MQ=255)
                                                 agctCGATAATGCCGTTATGATCGTCAGAAATTCCCAGCTCAGAGAAGGAGCACAGCATCCCTTCAGAc  <  1:2989078/66‑1 (MQ=255)
                                                 agctCGATAATGCCGTTATGATCGTCAGAAATTCCCAGCTCAGAGAAGGAGCACAGCATCCCTTCAGAc  <  1:483426/66‑1 (MQ=255)
                                                 agctCGATAATGCCGTTATGATCGTCAGAAATTCCCAGCTCAGAGAAGGAGCACAGCATCCCTTCAGAc  <  1:2585884/66‑1 (MQ=255)
                                                 agctCGATAATGCCGTTATGATCGTCAGAAATTCCCAGCTCAGAGAAGGAGCACAGCATCCCTTCAGAc  <  1:2567512/66‑1 (MQ=255)
                                                 agctCGATAATGCCGTTATGATCGTCAGAAATTCCCAGCTCAGAGAAGGAGCACAGCATCCCTTCAGAc  <  1:655831/66‑1 (MQ=255)
                                                   ctCGATAATGCCGTTATGATCGTCAGAAATTCCCAGCTCAGAGAAGGAGCACAGCATCCCTTCAGAc  <  1:1455608/66‑1 (MQ=255)
                                                   ctCGATAATGCCGTTATGATCGTCAGAAATTCCCAGCTCAGAGAAGGAGCACAGCATCCCTTCAGAc  <  1:2381613/66‑1 (MQ=255)
                                                                |                                                     
AAGTTTCAGGTATTCACGGATATCGGTGCCAATCGGCGCATCCGCAGGCAGTTCGATAATGCCGCTGTGATCGTCAGAAATGCCCAGTTCAGAGAAGGAGCACAGCATCCCTTCAGAC  >  minE/1125177‑1125294

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: