| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,125,258 | G→T | 46.5% | G126G (GGC→GGA) | pheT ← | phenylalanine tRNA synthetase, beta subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,125,258 | 0 | G | T | 46.5% | 12.8 / 41.5 | 43 | G126G (GGC→GGA) | pheT | phenylalanine tRNA synthetase, beta subunit |
| Reads supporting (aligned to +/- strand): ref base G (14/9); new base T (8/12); total (22/21) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.27e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.60e-01 | |||||||||||
CACGGATATCGGTGCCAATCGGCGCATCCGCAGGCAGTTCGATAATGCCGCTGTGATCGTCAGAAATGCCCAGTTCAGAGAAGGAGCACAGCATCCCTTCAGAC > minE/1125191‑1125294 | cACGGATATCGGTGCCAATCGGCGCATCCGCAGGCAGTTCGATAATGCCGCTGTGATCGTCAGAAATg > 1:564620/1‑68 (MQ=255)cACGGATATCGGTGCCAATCGGCGCATCCGCAGGCAGTTCGATAATGCCGCTGTGATCGTCAGAAATg > 1:1652933/1‑68 (MQ=255) cGGATATCGGTGCCAATCGGCGCATCCGCAGGCAGTTCGATAATGCCGCTGTGATCTTCAGAAATGcc < 1:3323942/68‑1 (MQ=255) cGGATATCGGTGCCAATCGGCGCATCCGCAGGCAGTTCGATAATGCCGCTGTGATCGTCAGAAATGcc < 1:1724095/68‑1 (MQ=255) cGGATATCGGTGCCAATCGGCGCATCCGCAGGCAGTTCGATAATGCCGCTGTGATCGTCAGAAATGcc < 1:2530374/68‑1 (MQ=255) cGGATATCGGTGCCAATCGGCGCATCCGCAGGCAGTTCGATAATGCCGCTGTGATCGTCAGAAATGcc < 1:2755185/68‑1 (MQ=255) cGGATATCGGTGCCAATCGGCGCATCCGCAGGCAGTTCGATAATGCCGCTGTGATCGTCAGAAATGcc < 1:3164256/68‑1 (MQ=255) cGGATATCGGTGCCAATCGGCGCATCCGCAGGCAGTTCGATAATGCCGCTGTGATCGTCAGAAATGcc < 1:1725806/68‑1 (MQ=255) cGGATATCGGTGCCAATCGGCGCATCCGCAGGCAGTTCGATAATGCCGCTGTGATCGTCAGAAATGcc < 1:3361924/68‑1 (MQ=255) cGGATATCGGTGCCAATCGGCGCATCCGCAGGCAGTTCGATAATGCCGCTGTGATCGTCAGAAATGcc < 1:3414831/68‑1 (MQ=255) tCGGTGCCAATCGGCGCATCCGCAGGCAGTTCGATAATGCCGCTGTGATCGTCAGAAATGCCCAGtt > 1:3311181/1‑67 (MQ=255) tCGGTGCCAATCGGCGCATCCGCAGGCAGTTCGATAATGCCGCTGTGATCGTCAGAAATGCCCAGtt > 1:2162765/1‑67 (MQ=255) tCGGTGCCAATCGGCGCATCCGCAGGCAGTTCGATAATGCCGCTGTGATCGTCAGAAATGCCCAGtt > 1:694876/1‑67 (MQ=255) cGGCGCATCCGCAGGCAGTTCGATAATGCCGCTGTGATCGTCAGAAATGCCCAGTTCAGAGAAGGAg > 1:2731303/1‑67 (MQ=255) cGGCGCATCCGCAGGCAGTTCGATAATGCCGCTGTGATCGTCAGAAATGCCCAGTTCAGAGAAGGAg > 1:30896/1‑67 (MQ=255) cGGCGCATCCGCAGGCAGTTCGATAATGCCGCTGTGATCGTCAGAAATGCCCAGTTCAGAGAAGGAg > 1:298326/1‑67 (MQ=255) cGGCGCATCCGCAGGCAGTTCGATAATGCCGCTGTGATCGTCAGAAATGCCCAGTTCAGAGAAGGAg > 1:1076854/1‑67 (MQ=255) cGGCGCATCCGCAGGCAGTTCGATAATGCCGCTGTGATCGTCAGAAATGCCCAGTTCAGAGAAGGAg > 1:1323974/1‑67 (MQ=255) cGGCGCATCCGCAGGCAGTTCGATAATGCCGCTGTGATCGTCAGAAATGCCCAGTTCAGAGAAGGAg > 1:2015503/1‑67 (MQ=255) cGGCGCATCCGCAGGCAGTTCGATAATGCCGCTGTGATCGTCAGAAATGCCCAGTTCAGAGAAGGAg > 1:2094388/1‑67 (MQ=255) ggCGCATCCGCAGGCAGTTCGATAATGCCGCTGTGATCGTCAGAAATGCCCAGTTCAGAGAAGGAGcac > 1:428574/1‑69 (MQ=255) ccGCAGGCAGCTCGATAATGCCGTTATGATCGTCAGAAATTCCCAGCTCagag > 1:537066/1‑53 (MQ=25) ccGCAGGCAGCTCGATAATGCCGTTATGATCGTCAGAAATTCCCAGCTCagag > 1:2916455/1‑53 (MQ=25) ccGCAGGCAGCTCGATAATGCCGTTATGATCGTCAGAAATTCCCAGCTCagag > 1:3088946/1‑53 (MQ=25) ccGCAGGCAGCTCGATAATGCCGTTATGATCGTCAGAAATTCCCAGCTCagag > 1:1498079/1‑53 (MQ=25) ccGCAGGCAGCTCGATAATGCCGTTATGATCGTCAGAAATTCCCAGCTCagag > 1:2477322/1‑53 (MQ=25) ccGCAGGCAGCTCGATAATGCCGTTATGATCGTCAGAAATTCCCAGCTCagag > 1:3264922/1‑53 (MQ=25) ccGCAGGCAGCTCGATAATGCCGTTATGATCGTCAGAAATTCCCAGCTCagag > 1:3306439/1‑53 (MQ=25) ccGCAGGCAGCTCGATAATGCCGTTATGATCGTCAGAAATTCCCAGCTCagag > 1:3341988/1‑53 (MQ=25) cGCAGGCAGTTCGATAATGCCGCTGTGATCGTCAGAAATGcc < 1:2600140/42‑1 (MQ=255) cGCAGGCAGTTCGATAATGCCGCTGTGATCGTCAGAAATGCCCAGTTCAGAGAAGGAGCACAGCATccc > 1:233506/1‑69 (MQ=255) cGCAGGCAGTTCGATAATGCCGCTGTGATCGTCAGAAATGCCCAGTTCAGAGAAGGAGCACAGCATccc > 1:1722096/1‑69 (MQ=255) cGCAGGCAGTTCGATAATGCCGCTGTGATCGTCAGAAATGCCCAGTTCAGAGAAGGAGCACAGCATccc > 1:1717746/1‑69 (MQ=255) agctCGATAATGCCGTTATGATCGTCAGAAATTCCCAGCTCAGAGAAGGAGCACAGCATCCCTTCAGAc < 1:655831/66‑1 (MQ=255) agctCGATAATGCCGTTATGATCGTCAGAAATTCCCAGCTCAGAGAAGGAGCACAGCATCCCTTCAGAc < 1:483426/66‑1 (MQ=255) agctCGATAATGCCGTTATGATCGTCAGAAATTCCCAGCTCAGAGAAGGAGCACAGCATCCCTTCAGAc < 1:3180252/66‑1 (MQ=255) agctCGATAATGCCGTTATGATCGTCAGAAATTCCCAGCTCAGAGAAGGAGCACAGCATCCCTTCAGAc < 1:1484348/66‑1 (MQ=255) agctCGATAATGCCGTTATGATCGTCAGAAATTCCCAGCTCAGAGAAGGAGCACAGCATCCCTTCAGAc < 1:173811/66‑1 (MQ=255) agctCGATAATGCCGTTATGATCGTCAGAAATTCCCAGCTCAGAGAAGGAGCACAGCATCCCTTCAGAc < 1:1762375/66‑1 (MQ=255) agctCGATAATGCCGTTATGATCGTCAGAAATTCCCAGCTCAGAGAAGGAGCACAGCATCCCTTCAGAc < 1:3073148/66‑1 (MQ=255) agctCGATAATGCCGTTATGATCGTCAGAAATTCCCAGCTCAGAGAAGGAGCACAGCATCCCTTCAGAc < 1:2989078/66‑1 (MQ=255) agctCGATAATGCCGTTATGATCGTCAGAAATTCCCAGCTCAGAGAAGGAGCACAGCATCCCTTCAGAc < 1:2585884/66‑1 (MQ=255) agctCGATAATGCCGTTATGATCGTCAGAAATTCCCAGCTCAGAGAAGGAGCACAGCATCCCTTCAGAc < 1:2567512/66‑1 (MQ=255) ctCGATAATGCCGTTATGATCGTCAGAAATTCCCAGCTCAGAGAAGGAGCACAGCATCCCTTCAGAc < 1:1455608/66‑1 (MQ=255) ctCGATAATGCCGTTATGATCGTCAGAAATTCCCAGCTCAGAGAAGGAGCACAGCATCCCTTCAGAc < 1:2381613/66‑1 (MQ=255) | CACGGATATCGGTGCCAATCGGCGCATCCGCAGGCAGTTCGATAATGCCGCTGTGATCGTCAGAAATGCCCAGTTCAGAGAAGGAGCACAGCATCCCTTCAGAC > minE/1125191‑1125294 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |