| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,125,725 | A→G | 48.9% | T303T (ACT→ACC) | pheS ← | phenylalanine tRNA synthetase, alpha subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,125,725 | 0 | A | G | 48.9% | ‑5.8 / 81.8 | 88 | T303T (ACT→ACC) | pheS | phenylalanine tRNA synthetase, alpha subunit |
| Reads supporting (aligned to +/- strand): ref base A (26/19); new base G (22/21); total (48/40) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.69e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.80e-01 | |||||||||||
TGAGGAAACGCAGATCGTTTTCGAAGAATGAACGCAGGTCGGTGACGCCGTAACGCAACATAGTCAGACGCTCCATCCCCATCCCGAAGGCGAAACCAGAGTAAACTTCCGGGTCGATGCCAACGT > minE/1125664‑1125789 | tGAGGAAACGCAGATCGTTTTCGAAGAATGAACGCAGGTCGGTGACGCCGTAACGCAACATGGTCAGa > 1:412437/1‑68 (MQ=255)tGAGGAAACGCAGATCGTTTTCGAAGAATGAACGCAGGTCGGTGACGCCGTAACGCAACATGGTCAGAc > 1:3111952/1‑69 (MQ=255)tGAGGAAACGCAGATCGTTTTCGAAGAATGAACGCAGGTCGGTGACGCCGTAACGCAACATGGTCAGAc > 1:2155064/1‑69 (MQ=255)tGAGGAAACGCAGATCGTTTTCGAAGAATGAACGCAGGTCGGTGACGCCGTAACGCAACATGGTCAGAc > 1:226183/1‑69 (MQ=255)tGAGGAAACGCAGATCGTTTTCGAAGAATGAACGCAGGTCGGTGACGCCGTAACGCAACATGGTCAGAc > 1:2022187/1‑69 (MQ=255)tGAGGAAACGCAGATCGTTTTCGAAGAATGAACGCAGGTCGGTGACGCCGTAACGCAACATGGTCAGAc > 1:2317525/1‑69 (MQ=255)tGAGGAAACGCAGATCGTTTTCGAAGAATGAACGCAGGTCGGTGACGCCGTAACGCAACATGGTCAGAc > 1:2996065/1‑69 (MQ=255)tGAGGAAACGCAGATCGTTTTCGAAGAATGAACGCAGGTCGGTGACGCCGTAACGCAACATGGTCAGAc > 1:1955445/1‑69 (MQ=255)tGAGGAAACGCAGATCGTTTTCGAAGAATGAACGCAGGTCGGTGACGCCGTAACGCAACATGGTCAGAc > 1:627611/1‑69 (MQ=255)tGAGGAAACGCAGATCGTTTTCGAAGAATGAACGCAGGTCGGTGACGCCGTAACGCAACATGGTCAGAc > 1:299612/1‑69 (MQ=255)tGAGGAAACGCAGATCGTTTTCGAAGAATGAACGCAGGTCGGTGACGCCGTAACGCAACATGGTCAGAc > 1:3029328/1‑69 (MQ=255)tGAGGAAACGCAGATCGTTTTCGAAGAATGAACGCAGGTCGGTGACGCCGTAACGCAACATGGTCAGAc > 1:3170654/1‑69 (MQ=255)tGAGGAAACGCAGATCGTTTTCGAAGAATGAACGCAGGTCGGTGACGCCGTAACGCAACATGGTCAGAc > 1:1526216/1‑69 (MQ=255)tGAGGAAACGCAGATCGTTTTCGAAGAATGAACGCAGGTCGGTGACGCCGTAACGCAACATGGTCAGAc > 1:3184068/1‑69 (MQ=255)tGAGGAAACGCAGATCGTTTTCGAAGAATGAACGCAGGTCGGTGACGCCGTAACGCAACATGGTCAGAc > 1:3216498/1‑69 (MQ=255)tGAGGAAACGCAGATCGTTTTCGAAGAATGAACGCAGGTCGGTGACGCCGTAACGCAACATGGTCAGAc > 1:2621510/1‑69 (MQ=255)tGAGGAAACGCAGATCGTTTTCGAAGAATGAACGCAGGTCGGTGACGCCGTAACGCAACATGGTCAGAc > 1:2841219/1‑69 (MQ=255)tGAGGAAACGCAGATCGTTTTCGAAGAATGAACGCAGGTCGGTGACGCCGTAACGCAACATGGTCAGAc > 1:27006/1‑69 (MQ=255)tGAGGAAACGCAGATCGTTTTCGAAGAATGAACGCAGGTCGGTGACGCCGTAACGCAACATGGTCAGAc > 1:2828488/1‑69 (MQ=255)tGAGGAAACGCAGATCGTTTTCGAAGAATGAACGCAGGTCGGTGACGCCGTAACGCAACATGGTCAGAc > 1:564741/1‑69 (MQ=255)tGAGGAAACGCAGATCGTTTTCGAAGAATGAACGCAGGTCGGTGACGCCGTAACGCAACATGGTCAGAc > 1:2786973/1‑69 (MQ=255)tGAGGAAACGCAGATCATTTTCGAAGAATGAACGCAGGTCGGTGACGCCGTAACGCAACATGGTCAGAc > 1:3338457/1‑69 (MQ=255) cGCAGATCGTTTTCGAAGAATGAACGCAGGTCGGTGACGCCGTAACGCAACATAGTCAGACGCTccatc < 1:525297/69‑1 (MQ=255) cGCAGATCGTTTTCGAAGAATGAACGCAGGTCGGTGACGCCGTAACGCAACATAGTCAGACGCTccatc < 1:2571930/69‑1 (MQ=255) cGCAGATCGTTTTCGAAGAATGAACGCAGGTCGGTGACGCCGTAACGCAACATAGTCAGACGCTccatc < 1:3400732/69‑1 (MQ=255) cGCAGATCGTTTTCGAAGAATGAACGCAGGTCGGTGACGCCGTAACGCAACATAGTCAGACGCTccatc < 1:508297/69‑1 (MQ=255) cGCAGATCGTTTTCGAAGAATGAACGCAGGTCGGTGACGCCGTAACGCAACATAGTCAGACGCTccatc < 1:1624027/69‑1 (MQ=255) cGCAGATCGTTTTCGAAGAATGAACGCAGGTCGGTGACGCCGTAACGCAACATAGTCAGACGCTccatc < 1:551802/69‑1 (MQ=255) tCGTTTTCGAAGAATGAACGCAGGTCGGTGACGCCGTAACGCAACATAGTCAGACGCTccatccccatc > 1:1346559/1‑69 (MQ=255) tCGTTTTCGAAGAATGAACGCAGGTCGGTGACGCCGTAACGCAACATAGTCAGACGCTccatccccatc > 1:3297231/1‑69 (MQ=255) tCGTTTTCGAAGAATGAACGCAGGTCGGTGACGCCGTAACGCAACATAGTCAGACGCTccatccccatc > 1:2598896/1‑69 (MQ=255) tCGTTTTCGAAGAATGAACGCAGGTCGGTGACGCCGTAACGCAACATAGTCAGACGCTccatccccatc > 1:1785212/1‑69 (MQ=255) cGTTTTCGAAGAATGAACGCAGGTCGGTGACGCCGTAAGGCAACATAGTCAGACGCTc > 1:2296056/1‑58 (MQ=255) cGTTTTCGAAGAATGAACGCAGGTCGGTGACGCCGTAACGCAACATAGTCAGACGCTc > 1:3346554/1‑58 (MQ=255) cGTTTTCGAAGAATGAACGCAGGTCGGTGACGCCGTAACGCAACATAGTCAGACGCTc > 1:3335143/1‑58 (MQ=255) cGTTTTCGAAGAATGAACGCAGGTCGGTGACGCCGTAACGCAACATAGTCAGACGCTc > 1:2411361/1‑58 (MQ=255) cGTTTTCGAAGAATGAACGCAGGTCGGTGACGCCGTAACGCAACATAGTCAGACGCTc > 1:1849854/1‑58 (MQ=255) cGTTTTCGAAGAATGAACGCAGGTCGGTGACGCCGTAACGCAACATAGTCAGACGCTc > 1:1999637/1‑58 (MQ=255) cGTTTTCGAAGAATGAACGCAGGTCGGTGACGCCGTAACGCAACATAGTCAGACGCTc > 1:2324649/1‑58 (MQ=255) cGTTTTCGAAGAATGAACGCAGGTCGGTGACGCCGTAACGCAACATAGTCAGACGCTc > 1:2013815/1‑58 (MQ=255) cGTTTTCGAAGAATGAACGCAGGTCGGTGACGCCGTAACGCAACATAGTCAGACGCTc > 1:22909/1‑58 (MQ=255) cGTTTTCGAAGAATGAACGCAGGTCGGTGACGCCGTAACGCAACATAGTCAGACGCTc > 1:2287243/1‑58 (MQ=255) cGTTTTCGAAGAATGAACGCAGGTCGGTGACGACGTAACGCAACATAGTCAGACGCTc > 1:2551757/1‑58 (MQ=255) agaaTGAACGCAGGTCGGTGACGCCGTAACGCAACATAGTCAGACGCTCCATCCCCATCCCGAAGGCGa < 1:40073/69‑1 (MQ=255) gaaTGAACGCAGGTCGGTGACGCCGTAACGCAACATAGTCAGACGCTCCATCCCCATCCCGAAGGCGaa < 1:3277515/69‑1 (MQ=255) gaaTGAACGCAGGTCGGTGACGCCGTAACGCAACATAGTCAGACGCTCCATCCCCATCCCGAAGGCGaa < 1:1141196/69‑1 (MQ=255) gaaTGAACGCAGGTCGGTGACGCCGTAACGCAACATAGTCAGACGCTCCATCCCCATCCCGAAGGCGaa < 1:381827/69‑1 (MQ=255) gaaTGAACGCAGGTCGGTGACGCCGTAACGCAACATAGTCAGACGCTCCATCCCCATCCCGAAGGCGaa < 1:555626/69‑1 (MQ=255) gaaTGAACGCAGGTCGGTGACGCCGTAACGCAACATAGTCAGACGCTCCATCCCCATCCCGAAGGCGaa < 1:1431942/69‑1 (MQ=255) gaaTGAACGCAGGTCGGTGACGCCGTAACGCAACATAGTCAGACGCTCCATCCCCATCCCGAAGGCGaa < 1:145297/69‑1 (MQ=255) gaaTGAACGCAGGTCGGTGACGCCGTAACGCAACATAGTCAGACGCTCCATCCCCATCCCGAAGGCGaa < 1:1479591/69‑1 (MQ=255) gaaTGAACGCAGGTCGGTGACGCCGTAACGCAACATAGTCAGACGCTCCATCCCCATCCCGAAGGCGaa < 1:1517380/69‑1 (MQ=255) gaaTGAACGCAGGTCGGTGACGCCGTAACGCAACATAGTCAGACGCTCCATCCCCATCCCGAAGGCGaa < 1:1612092/69‑1 (MQ=255) gaaTGAACGCAGGTCGGTGACGCCGTAACGCAACATAGTCAGACGCTCCATCCCCATCCCGAAGGCGaa < 1:540427/69‑1 (MQ=255) gaaTGAACGCAGGTCGGTGACACCGTAACGCAACATAGTCAGACGCTCCATCCCCATCCCGAAGGCGaa < 1:3358687/69‑1 (MQ=255) aTGAACGCAGGTCGGTGACGCCGTAACGCAACATAGTCAGACGCTCCATCCCCATCCCGATGGCGaa < 1:436025/67‑1 (MQ=255) gAACGCAGGTCGGTGACGCCGTAACGCAACATGGTCAGACGCTCCATCCCCATCCCGAAGGCGAAACCa < 1:177201/69‑1 (MQ=255) gAACGCAGGTCGGTGACGCCGTAACGCAACATGGTCAGACGCTCCATCCCCATCCCGAAGGCGAAACCa < 1:3119907/69‑1 (MQ=255) gAACGCAGGTCGGTGACGCCGTAACGCAACATGGTCAGACGCTCCATCCCCATCCCGAAGGCGAAACCa < 1:2978182/69‑1 (MQ=255) gAACGCAGGTCGGTGACGCCGTAACGCAACATGGTCAGACGCTCCATCCCCATCCCGAAGGCGAAACCa < 1:2945251/69‑1 (MQ=255) gAACGCAGGTCGGTGACGCCGTAACGCAACATGGTCAGACGCTCCATCCCCATCCCGAAGGCGAAACCa < 1:3240534/69‑1 (MQ=255) gAACGCAGGTCGGTGACGCCGTAACGCAACATGGTCAGACGCTCCATCCCCATCCCGAAGGCGAAACCa < 1:2399644/69‑1 (MQ=255) gAACGCAGGTCGGTGACGCCGTAACGCAACATGGTCAGACGCTCCATCCCCATCCCGAAGGCGAAACCa < 1:2413618/69‑1 (MQ=255) gAACGCAGGTCGGTGACGCCGTAACGCAACATGGTCAGACGCTCCATCCCCATCCCGAAGGCGAAACCa < 1:40077/69‑1 (MQ=255) gAACGCAGGTCGGTGACGCCGTAACGCAACATGGTCAGACGCTCCATCCCCATCCCGAAGGCGAAACCa < 1:1385260/69‑1 (MQ=255) gAACGCAGGTCGGTGACGCCGTAACGCAACATGGTCAGACGCTCCATCCCCATCCCGAAGGCGAAACCa < 1:377341/69‑1 (MQ=255) aaCGCAGGTCGGTGACGCCGTAACGCAACATGGTCAGACGCTCCATCCCCATCCCGAAGGCGAAACCa < 1:1570916/68‑1 (MQ=255) cGGTGACGCCGTAACGCAACATAGTCAGACGCTCCATCCCCATCCCGAAGGCGAAACCAGAGTAAACtt > 1:593136/1‑69 (MQ=255) cGGTGACGCCGTAACGCAACATAGTCAGACGCTCCATCCCCATCCCGAAGGCGAAACCAGAGTAAACtt > 1:2666897/1‑69 (MQ=255) cGGTGACGCCGTAACGCAACATAGTCAGACGCTCCATCCCCATCCCGAAGGCGAAACCAGAGTAAACtt > 1:1773534/1‑69 (MQ=255) gACGCCGTAACGCAACATAGTCAGACGCTCCATCCCCATCCCGAAGGCGAAACCAGAGTAAACTTCCgg > 1:2591436/1‑69 (MQ=255) gACGCCGTAACGCAACATAGTCAGACGCTCCATCCCCATCCCGAAGGCGAAACCAGAGTAAACTTCCgg > 1:1308503/1‑69 (MQ=255) cGCCGTAACGCAACATGGTCAGACGCTCCATCCCCATCCCGAAGGCGAAACCa < 1:648370/53‑1 (MQ=255) cGCCGTAACGCAACATGGTCAGACGCTCCATCCCCATCCCGAAGGCGAAACCa < 1:381075/53‑1 (MQ=255) cGCCGTAACGCAACATGGTCAGACGCTCCATCCCCATCCCGAAGGCGAAACCa < 1:105785/53‑1 (MQ=255) cGCCGTAACGCAACATGGTCAGACGCTCCATCCCCATCCCGAAGGCGAAACCa < 1:2688257/53‑1 (MQ=255) cGCCGTAACGCAACATGGTCAGACGCTCCATCCCCATCCCGAAGGCGAAACCa < 1:2360571/53‑1 (MQ=255) cGCCGTAACGCAACATGGTCAGACGCTCCATCCCCATCCCGAAGGCGAAACCa < 1:484166/53‑1 (MQ=255) cGCCGTAACGCAACATGGTCAGACGCTCCATCCCCATCCCGAAGGCGAAACCa < 1:2194089/53‑1 (MQ=255) cGCCGTAACGCAACATGGTCAGACGCTCCATCCCCATCCCGAAGGCGAAACCa < 1:2037749/53‑1 (MQ=255) cGCCGTAACGCAACATGGTCAGACGCTCCATCCCCATCCCGAAGGCGAAACCa < 1:1728311/53‑1 (MQ=255) cGCCGTAACGCAACATGGTCAGACGCTCCATCCCCATCCCGAAGGCGAAACCa < 1:1391992/53‑1 (MQ=255) aCATAGTCAGACGCTCCATCCCCATCCCGAAGGCGAAACCAGAGTAAACTTCCGGGTCGATGCCAACGt > 1:90434/1‑69 (MQ=255) aCATAGTCAGACGCTCCATCCCCATCCCGAAGGCGAAACCAGAGTAAACTTCCGGGTCGATGCCAACGt > 1:2701055/1‑69 (MQ=255) aCATAGTCAGACGCTCCATCCCCATCCCGAAGGCGAAACCAGAGTAAACTTCCGGGTCGATGCCAACGt > 1:2865958/1‑69 (MQ=255) aCATAGTCAGACGCTCCATCCCCATCCCGAAGGCGAAACCAGAGTAAACTTCCGGGTCGATGCCAACGt > 1:2849043/1‑69 (MQ=255) aCATAGTCAGACGCTCCATCCCCATCCCGAAGGCGAAACCAGAGTAAACTTCCGGGTCGATGCCAACGt > 1:2713490/1‑69 (MQ=255) aCATAGTCAGACGCTCCATCCCCATCCCGAAGGCGAAACCAGAGTAAACTTCAGGGTCGATGCCAACGt > 1:973622/1‑69 (MQ=255) | TGAGGAAACGCAGATCGTTTTCGAAGAATGAACGCAGGTCGGTGACGCCGTAACGCAACATAGTCAGACGCTCCATCCCCATCCCGAAGGCGAAACCAGAGTAAACTTCCGGGTCGATGCCAACGT > minE/1125664‑1125789 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |