| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,126,349 | A→G | 77.6% | S95S (TCT→TCC) | pheS ← | phenylalanine tRNA synthetase, alpha subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,126,349 | 0 | A | G | 77.6% | 50.1 / 9.1 | 40 | S95S (TCT→TCC) | pheS | phenylalanine tRNA synthetase, alpha subunit |
| Reads supporting (aligned to +/- strand): ref base A (2/7); new base G (20/11); total (22/18) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.35e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.44e-01 | |||||||||||
GTCGATGGTACGGGTAACCGGATGCAGACCGCCGTTTTCAATGCGACGACCTGGCAGAGAGACATCAATCGTTTCCGCCGCCAGACGCGCATTCAGTGCAGCGCTTTCCAGTTCCGCTTTACGCG > minE/1126292‑1126416 | gTCGATGGTACGGGTCACCGGATGCAGCCCGCCGTTTTCAATGCGACGACCCGGCAGGGAGACATCaa < 1:1332349/68‑1 (MQ=255)gTCGATGGTACGGGTCACCGGATGCAGCCCGCCGTTTTCAATGCGACGACCCGGCAGGGAGACATCaa < 1:1379135/68‑1 (MQ=255)gTCGATGGTACGGGTCACCGGATGCAGCCCGCCGTTTTCAATGCGACGACCCGGCAGGGAGACATCaa < 1:879628/68‑1 (MQ=255)gTCGATGGTACGGGTCACCGGATGCAGCCCGCCGTTTTCAATGCGACGACCCGGCAGGGAGACATCaa < 1:1544558/68‑1 (MQ=255)gTCGATGGTACGGGTCACCGGATGCAGCCCGCCGTTTTCAATGCGACGACCCGGCAGGGAGACATCaa < 1:171666/68‑1 (MQ=255)gTCGATGGTACGGGTCACCGGATGCAGCCCGCCGTTTTCAATGCGACGACCCGGCAGGGAGACATCaa < 1:75833/68‑1 (MQ=255)gTCGATGGTACGGGTCACCGGATGCAGCCCGCCGTTTTCAATGCGACGACCCGGCAGGGAGACATCaa < 1:2194106/68‑1 (MQ=255)gTCGATGGTACGGGTCACCGGATGCAGCCCGCCGTTTTCAATGCGACGACCCGGCAGGGAGACATCaa < 1:561889/68‑1 (MQ=255) tCGATGGTACGGGTCACCGGATGCAGCCCGCCGTTTTCAATGCGACGACCCGGCAGGGAGACATCaa < 1:1073963/67‑1 (MQ=255) tCGATGGTACGGGTCACCGGATGCAGCCCGCCGTTTTCAATGCGACGACCCGGCAGGGAGACATCaa < 1:882632/67‑1 (MQ=255) gggTCACCGGATGCAGCCCGCCGTTTTCAATGCGCCGACCCGGCAGGGAGACATCAATCGTTTccgcc > 1:2966534/1‑68 (MQ=255) gggTCACCGGATGCAGCCCGCCGTTTTCAATGCGACGACCCGGCAGGGAGACATc > 1:731398/1‑55 (MQ=255) gggTCACCGGATGCAGCCCGCCGTTTTCAATGCGACGACCCGGCAGGGAGACATCAATCGttt > 1:2339030/1‑63 (MQ=255) gggTCACCGGATGCAGCCCGCCGTTTTCAATGCGACGACCCGGCAGGGAGACATCAATCGTTTccgcc > 1:2726898/1‑68 (MQ=255) gggTCACCGGATGCAGCCCGCCGTTTTCAATGCGACGACCCGGCAGGGAGACATCAATCGTTTccgcc > 1:1262032/1‑68 (MQ=255) gggTCACCGGATGCAGCCCGCCGTTTTCAATGCGACGACCCGGCAGGGAGACATCAATCGTTTccgcc > 1:3329/1‑68 (MQ=255) gggTCACCGGATGCAGCCCGCCGTTTTCAATGCGACGACCCGGCAGGGAGACATCAATCGTTTccgcc > 1:3403063/1‑68 (MQ=255) gggTCACCGGATGCAGCCCGCCGTTTTCAATGCGACGACCCGGCAGGGAGACATCAATCGTTTccgcc > 1:526925/1‑68 (MQ=255) gggTCACCGGATGCAGCCCGCCGTTTTCAATGCGACGACCCGGCAGGGAGACATCAATCGTTTccgcc > 1:740394/1‑68 (MQ=255) gggTCACCGGATGCAGCCCGCCGTTTTCAATGCGACGACCCGGCAGGGAGACATCAATCGTTTccgcc > 1:812173/1‑68 (MQ=255) gggTCACCGGATGCAGCCCGCCGTTTTCAATGCGACGACCCGGCAGGGAGACATCAATCGTTTccgcc > 1:2604776/1‑68 (MQ=255) gggTCACCGGATGCAGCCCGCCGTTTTCAATGCGACGACCCGGCAGGGAGACATCAATCGTTTccgcc > 1:1489509/1‑68 (MQ=255) gggTCACCGGATGCAGCCCGCCGTTTTCAATGCGACGACCCGGCAGGGAGACATCAATCGTTTccgcc > 1:1643913/1‑68 (MQ=255) gggTCACCGGATGCAGCCCGCCGTTTTCAATGCGACGACCCGGCAGGGAGACATCAATCGTTTccgcc > 1:1840568/1‑68 (MQ=255) gggTCACCGGATGCAGCCCGCCGTTTTCAATGCGACGACCCGGCAGGGAGACATCAATCGTTTccgcc > 1:1926931/1‑68 (MQ=255) gggTCACCGGATGCAGCCCGCCGTTTTCAATGCGACGACCCGGCAGGGAGACATCAATCGTTTccgcc > 1:1998917/1‑68 (MQ=255) gggTCACCGGATGCAGCCCGCCGTTTTCAATGCGACGACCCGGCAGGGAGACATCAATCGTTTccgcc > 1:2267239/1‑68 (MQ=255) gggTCACCGGATGCAGCCCGCCGTTTTCAATGCGACGACCCGGCAGGGAGACATCAATCGTTTccgcc > 1:2498043/1‑68 (MQ=255) gggTCACCGGATGCAGCCCGCCGTTTTCAATGCGACGACCCGGCAGGGAGACATCAATCGTTTccgcc > 1:2599318/1‑68 (MQ=255) gggTCACCGGATGCAGCCAGCCGTTTTCAATGCGACGACCCGGCAGGGAGACATCAATCGTTTccgcc > 1:3399510/1‑68 (MQ=255) tcaCCGGATGCAGCCCGCCGTTTTCAATGCGACGACCCGGCAGGGAGACATCaa < 1:2658158/52‑1 (MQ=255) aCCGGATGCAGACCGCCGTTTTCAATGCGACGACCTGGCAGAGAGACATCAATCGTTTCCGCCGCCAGa < 1:3216484/69‑1 (MQ=255) aCCGGATGCAGACCGCCGTTTTCAATGCGACGACCTGGCAGAGAGACATCAATCGTTTCCGCCGCCAGa < 1:3052475/69‑1 (MQ=255) aCCGGATGCAGACCGCCGTTTTCAATGCGACGACCTGGCAGAGAGACATCAATCGTTTCCGCCGCCAGa < 1:1344406/69‑1 (MQ=255) ttCAATGCGACGACCTGGCAGAGAGACATCAATCGTTTCCGCCGCCAGACGCGCATTCAGTGCAGCGCt > 1:707778/1‑69 (MQ=255) ttCAATGCGACGACCTGGCAGAGAGACATCAATCGTTTCCGCCGCCAGACGCGCATTCAGTGCAGCGCt > 1:317231/1‑69 (MQ=255) tGCGACGACCTGGCAGAGAGACATCAATCGTTTCCGCCGCCAGACGCGCATTCAGTGCAGCGCTTTcc < 1:246495/68‑1 (MQ=255) tGCGACGACCTGGCAGAGAGACATCAATCGTTTCCGCCGCCAGACGCGCATTCAGTGCAGCGCTTTcc < 1:688593/68‑1 (MQ=255) tGCGACGACCTGGCAGAGAGACATCAATCGTTTCCGCCGCCAGACGCGCATTCAGTGCAGCGCTTTcc < 1:823230/68‑1 (MQ=255) tGCGACGACCTGGCAGAGAGACATCAATCGTTTCCGCCGCCAGACGCGCATTCAGTGCAGCGCTTTcc < 1:852092/68‑1 (MQ=255) agagaCATCAATCGTTTCCGCCGCCAGACGCGCATTCAGTGCAGCGCTTTCCAGTTCCGCTTTAcgcg < 1:2329357/68‑1 (MQ=255) | GTCGATGGTACGGGTAACCGGATGCAGACCGCCGTTTTCAATGCGACGACCTGGCAGAGAGACATCAATCGTTTCCGCCGCCAGACGCGCATTCAGTGCAGCGCTTTCCAGTTCCGCTTTACGCG > minE/1126292‑1126416 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |