| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,130,566 | G→A | 25.2% | intergenic (‑305/‑219) | thrS ← / → arpB | threonyl‑tRNA synthetase/ECK1718:JW5278:b1720; hypothetical protein, N‑ter fragment |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,130,566 | 0 | G | A | 25.2% | 92.2 / 24.8 | 75 | intergenic (‑305/‑219) | thrS/arpB | threonyl‑tRNA synthetase/ECK1718:JW5278:b1720; hypothetical protein, N‑ter fragment |
| Reads supporting (aligned to +/- strand): ref base G (43/13); new base A (12/7); total (55/20) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.68e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
AGTACACCAGCAAAACGCTTAAAGAACAACTGATAACATCACGGTGCGTCTGAAAGGTTTACTGCGAGCGGATTTACAGCAGCATAAACTGAATCCAGCAAAGTTCGTTGCGTGATTTGGCGGTAGT > minE/1130503‑1130629 | aGTACACCAGCAAAACGCTTAAAGAACAACTGATAACATCACGGTGCGTCTGAAAGGTTTACTGCGAg > 1:500422/1‑68 (MQ=255)aGTACACCAGCAAAACGCTTAAAGAACAACTGATAACATCACGGTGCGTCTGAAAGGTTTACTGCGAg > 1:1781430/1‑68 (MQ=255)aGTACACCAGCAAAACGCTTAAAGAACAACTGATAACATCACGGTGCGTCTGAAAGGTTTACTGCGAg > 1:1861403/1‑68 (MQ=255)aGTACACCAGCAAAACGCTTAAAGAACAACTGATAACATCACGGTGCGTCTGAAAGGTTTACTGCGAg > 1:1468584/1‑68 (MQ=255)aGTACACCAGCAAAACGCTTAAAGAACAACTGATAACATCACGGTGCGTCTGAAAGGTTTACTGCGAg > 1:2110232/1‑68 (MQ=255)aGTACACCAGCAAAACGCTTAAAGAACAACTGATAACATCACGGTGCGTCTGAAAGGTTTACTGCGAg > 1:2324302/1‑68 (MQ=255)aGTACACCAGCAAAACGCTTAAAGAACAACTGATAACATCACGGTGCGTCTGAAAGGTTTACTGCGAg > 1:841083/1‑68 (MQ=255)aGTACACCAGCAAAACGCTTAAAGAACAACTGATAACATCACGGTGCGTCTGAAAGGTTTACTGCGAg > 1:845061/1‑68 (MQ=255)aGTACACCAGCAAAACGCTTAAAGAACAACTGATAACATCACGGTGCGTCTGAAAGGTTTACTGCGAg > 1:988057/1‑68 (MQ=255) tACACCAGCAAAACGCTTAAAGAACAACTGATAACATCACGGTGCGTCTGAAAGGTTTACTGCGAGCgg > 1:697940/1‑69 (MQ=255) tACACCAGCAAAACGCTTAAAGAACAACTGATAACATCACGGTGCGTCTGAAAGGTTTACTGCGAGCgg < 1:657392/69‑1 (MQ=255) tACACCAGCAAAACGCTTAAAGAACAACTGATAACATCACGGTGCGTCTGAAAGGTTTACTGCGAGCgg < 1:1012977/69‑1 (MQ=255) tACACCAGCAAAACGCTTAAAGAACAACTGATAACATCACGGTGCGTCTGAAAGGTTTACTGCGAGCgg > 1:420541/1‑69 (MQ=255) tACACCAGCAAAACGCTTAAAGAACAACTGATAACATCACGGTGCGTCTGAAAGGTTTACTGCGAGCgg > 1:2743468/1‑69 (MQ=255) tACACCAGCAAAACGCTTAAAGAACAACTGATAACATCACGGTGCGTCTGAAAGGTTTACTGCGAGCgg > 1:249556/1‑69 (MQ=255) tACACCAGCAAAACGCTTAAAGAACAACTGATAACATCACGGTGCGTCTGAAAGGTTTACTGCGAGCgg < 1:906568/69‑1 (MQ=255) tACACCAGCAAAACGCTTAAAGAACAACTGATAACATCACGGTGCGTCTGAAAGGTTTACTGCGAGCgg < 1:931654/69‑1 (MQ=255) tACACCAGCAAAACGCTTAAAGAACAACTGATAACATCACGGTGCGTCTGAAAGGTTTACTGCGAGCgg > 1:994881/1‑69 (MQ=255) tACACCAGCAAAACGCTTAAAGAACAACTGATAACATCACGGTGCGTCTGAAAGGTTTACTGCGAGCgg > 1:1088658/1‑69 (MQ=255) caacTGATAACATCACGGTGCGTCTGAAAGGTTTACTGCGATCGGATTTACAGCAGCATAAACTGaa > 1:1114907/1‑67 (MQ=255) caacTGATAACATCACGGTGCGTCTGAAAGGTTTACTGCGAGCGGATTTACAGCAGCATAAACTGaa > 1:1012397/1‑67 (MQ=255) caacTGATAACATCACGGTGCGTCTGAAAGGTTTACTGCGAGCGGATTTACAGCAGCATAAACTGaa > 1:2413165/1‑67 (MQ=255) caacTGATAACATCACGGTGCGTCTGAAAGGTTTACTGCGAGCGGATTTACAGCAGCATAAACTGaa > 1:141753/1‑67 (MQ=255) caacTGATAACATCACGGTGCGTCTGAAAGGTTTACTGCGAGCGGATTTACAGCAGCATAAACTGaa > 1:3201131/1‑67 (MQ=255) caacTGATAACATCACGGTGCGTCTGAAAGGTTTACTGCGAGCGGATTTACAGCAGCATAAACTGaa > 1:3215493/1‑67 (MQ=255) caacTGATAACATCACGGTGCGTCTGAAAGGTTTACTGCGAGCGGATTTACAGCAGCATAAACTGaa > 1:3261624/1‑67 (MQ=255) caacTGATAACATCACGGTGCGTCTGAAAGGTTTACTGCGAGCGGATTTACAGCAGCATAAACTGaa > 1:2081962/1‑67 (MQ=255) caacTGATAACATCACGGTGCGTCTGAAAGGTTTACTGCGAGCGGATTTACAGCAGCATAAACTGaa > 1:430619/1‑67 (MQ=255) caacTGATAACATCACGGTGCGTCTGAAAGGTTTACTGCGAGCGGATTTACAGCAGCATAAACTGaa > 1:1521064/1‑67 (MQ=255) caacTGATAACATCACGGTGCGTCTGAAAGGTTTACTGCGAGCGGATTTACAGCAGCATAAACTGaa > 1:818348/1‑67 (MQ=255) caacTGATAACATCACGGTGCGTCTGAAAGGTTTACTGCGAGCGGATTTACAGCAGCATAAACTGa > 1:3416245/1‑66 (MQ=255) gATAACATCACGGTGCGTCTGAAAGGTTTACTGCGAGCGGATTTACAGCAGCATAAACTGAATCCa > 1:1742684/1‑66 (MQ=255) gATAACATCACGGTGCGTCTGAAAGGTTTACTGCGAGCGGATTTACAGCAGCATAAACTGAATCCAg > 1:1378977/1‑67 (MQ=255) gATAACATCACGGTGCGTCTGAAAGGTTTACTGCGAGCGGATTTACAGCAGCATAAACTGAATCCAg > 1:379810/1‑67 (MQ=255) gATAACATCACGGTGCGTCTGAAAGGTTTACTGCGAGCGGATTTACAGCAGCATAAACTGAATCCAg > 1:53356/1‑67 (MQ=255) gATAACATCACGGTGCGTCTGAAAGGTTTACTGCGAGCGGATTTACAGCAGCATAAACTGAATCCAg > 1:1191181/1‑67 (MQ=255) gATAACATCACGGTGCGTCTGAAAGGTTTACTGCGAGCGGATTTACAGCAGCATAAACTGAATCCAg > 1:3038494/1‑67 (MQ=255) gATAACATCACGGTGCGTCTGAAAGGTTTACTGCGAGCGGATTTACAGCAGCATAAACTGAATCCAg > 1:2393296/1‑67 (MQ=255) gATAACATCACGGTGCGTCTGAAAGGTTTACTGCGAGCGGATTTACAGCAGCATAAACTGAATCCAg > 1:1526996/1‑67 (MQ=255) gATAACATCACGGTGCGTCTGAAAGGTTTACTGCGAGCGGATTTACAGCAGCATAAACTGAATCCAg > 1:1445635/1‑67 (MQ=255) tAACATCACAGAGTGTCTGAAAGGTTTACTACGAGCGGATTTACAGCAGCATAAACTGAATCCAGCaaa > 1:142670/1‑69 (MQ=255) tAACATCACAGAGTGTCTGAAAGGTTTACTACGAGCGGATTTACAGCAGCATAAACTGAATCCAGCaaa > 1:2339889/1‑69 (MQ=255) tAACATCACAGAGTGTCTGAAAGGTTTACTACGAGCGGATTTACAGCAGCATAAACTGAATCCAGCaaa > 1:1735598/1‑69 (MQ=255) tAACATCACAGAGTGTCTGAAAGGTTTACTACGAGCGGATTTACAGCAGCATAAACTGAATCCAGCaaa > 1:2977756/1‑69 (MQ=255) tAACATCACAGAGTGTCTGAAAGGTTTACTACGAGCGGATTTACAGCAGCATAAACTGAATCCAGCaaa > 1:2991461/1‑69 (MQ=255) tAACATCACAGAGTGTCTGAAAGGTTTACTACGAGCGGATTTACAGCAGCATAAACTGAATCCAGCaaa > 1:3001247/1‑69 (MQ=255) tAACATCACAGAGTGTCTGAAAGGTTTACTACGAGCGGATTTACAGCAGCATAAACTGAATCCAGCaaa > 1:3427490/1‑69 (MQ=255) tAACATCACAGAGTGTCTGAAAGGTTTACTACGAGCGGATTTACAGCAGCATAAACTGAATCCAGCaaa > 1:206607/1‑69 (MQ=255) tAACATCACAGAGTGTCTGAAAGGTTTACTACGAGCGGATTTACAGCAGCATAAACTGAATCCAGCaaa > 1:314389/1‑69 (MQ=255) tAACATCACAGAGTGTCTGAAAGGTTTACTACGAGCGGATTTACAGCAGCATAAACTGAATCCAGCaaa > 1:1629596/1‑69 (MQ=255) tAACATCACAGAGTGTCTGAAAGGTTTACTACGAGCGGATTTACAGCAGCATAAACTGAATCCAGCaaa > 1:1097067/1‑69 (MQ=255) tAACATCACAGAGTGTCTGAAAGGTTTACTACGAGCGGATTTACAGCAGCATAAACTGAATCCAGCaaa > 1:198474/1‑69 (MQ=255) cACTGTGCGTCTGAAAGGTTTACTGCGAGCGGATTTACAGCAGCATAAACTg < 1:89573/52‑1 (MQ=255) cACGGTGCGTCTGAAAGGTTTACTGCGAGCGGATTTACAGCAGCATAAACTg < 1:1968784/52‑1 (MQ=255) cACGGTGCGTCTGAAAGGTTTACTGCGAGCGGATTTACAGCAGCATAAACTg < 1:1185232/52‑1 (MQ=255) cACGGTGCGTCTGAAAGGTTTACTGCGAGCGGATTTACAGCAGCATAAACTg < 1:1868625/52‑1 (MQ=255) cACGGTGCGTCTGAAAGGTTTACTGCGAGCGGATTTACAGCAGCATAAACTg < 1:1271627/52‑1 (MQ=255) cACGGTGCGTCTGAAAGGTTTACTGCGAGCGGATTTACAGCAGCATAAACTg < 1:1581811/52‑1 (MQ=255) cACGGTGCGTCTGAAAGGTTTACTGCGAGCGGATTTACAGCAGCATAAACTg < 1:2410799/52‑1 (MQ=255) aCGGTGCGTCTGAAAGGTTTACTGCGAGCGGATTTACAGCAGCATAAACTGAATCCAGCAAAGTTCGtt > 1:3396531/1‑69 (MQ=255) aCGGTGCGTCTGAAAGGTTTACTGCGAGCGGATTTACAGCAGCATAAACTGAATCCAGCAAAGTTCGtt > 1:1397119/1‑69 (MQ=255) aCGGTGCGTCTGAAAGGTTTACTGCGAGCGGATTTACAGCAGCATAAACTGAATCCAGCAAAGTTCGtt > 1:211800/1‑69 (MQ=255) aCGGTGCGTCTGAAAGGTTTACTGCGAGCGGATTTACAGCAGCATAAACTGAATCCAGCAAAGTTCGtt > 1:1614490/1‑69 (MQ=255) aCGGTGCGTCTGAAAGGTTTACTGCGAGCGGATTTACAGCAGCATAAACTGAATCCAGCAAAGTTCGtt > 1:1806005/1‑69 (MQ=255) aCGGTGCGTCTGAAAGGTTTACTGCGAGCGGATTTACAGCAGCATAAACTGAATCCAGCAAAGTTCGtt > 1:295362/1‑69 (MQ=255) gCGTCTGAAAGGTTTACTGCGAGCGGATTTACAGCAGCATAAACTGAATCCAGCAAAGTTCGTTGCGTg < 1:65828/69‑1 (MQ=255) tGAAAGGTTTACTACGAGCGGATTTACAGCAGCATTAACTGAATCCAGCAAAg < 1:1599079/53‑1 (MQ=255) tGAAAGGTTTACTACGAGCGGATTTACAGCAGCATAAACTGAATCCAGCAAAg < 1:688542/53‑1 (MQ=255) tGAAAGGTTTACTACGAGCGGATTTACAGCAGCATAAACTGAATCCAGCAAAg < 1:472002/53‑1 (MQ=255) tGAAAGGTTTACTACGAGCGGATTTACAGCAGCATAAACTGAATCCAGCAAAg < 1:3401713/53‑1 (MQ=255) tGAAAGGTTTACTACGAGCGGATTTACAGCAGCATAAACTGAATCCAGCAAAg < 1:1465459/53‑1 (MQ=255) tGAAAGGTTTACTACGAGCGGATTTACAGCAGCATAAACTGAATCCAGCAAAg < 1:2734828/53‑1 (MQ=255) tGAAAGGGTTACTACGAGCGGATTTACAGCAGCATAAACTGAATCCAGCAAAg < 1:3057294/53‑1 (MQ=255) ttACTGCGAGCGGATTTACAGCAGCATAAACTGAATCCAGCAAAGTTCGTTGCGTGATTTGGCGgtagt > 1:1371543/1‑69 (MQ=255) ttACTGCGAGCGGATTTACAGCAGCATAAACTGAATCCAGAAAAGTTCGTTGCGTGATTTGGCGgtagt < 1:358683/69‑1 (MQ=255) | AGTACACCAGCAAAACGCTTAAAGAACAACTGATAACATCACGGTGCGTCTGAAAGGTTTACTGCGAGCGGATTTACAGCAGCATAAACTGAATCCAGCAAAGTTCGTTGCGTGATTTGGCGGTAGT > minE/1130503‑1130629 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |