| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,133,310 | A→G | 52.2% | G155G (GGT→GGC) | ydiY ← | conserved hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,133,310 | 0 | A | G | 52.2% | 2.0 / 37.9 | 48 | G155G (GGT→GGC) | ydiY | conserved hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base A (17/6); new base G (9/16); total (26/22) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.08e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
ATAGCCCAGCGGCTGGGTTTCACTGGCATTGTCGGTATATTTGTCGTAACGCACACCTGGACCGAATTCAAAGCGGAAACTGTGTACCGGGCCATTCAAAAACTGACGACCAT > minE/1133250‑1133362 | aTAGCCCAGCGGCTGGGTTTCACTGGCATTGTCGGTATATTTGTCGTAACGCACACCTGGACCGAAtt > 1:1224260/1‑68 (MQ=255)aTAGCCCAGCGGCTGGGTTTCACTGGCATTGTCGGTATATTTGTCGTAACGCACACCTGGACCGAAtt > 1:2196674/1‑68 (MQ=255)aTAGCCCAGCGGCTGGGTTTCACTGGCATTGTCGGTATATTTGTCGTAACGCACACCTGGACCGAAtt > 1:2107146/1‑68 (MQ=255)aTAGCCCAGCGGCTGGGTTTCACTGGCATTGTCGGTATATTTGTCGTAACGCACACCTGGACCGAAtt > 1:29498/1‑68 (MQ=255) tAGCCCAGCGGCTGGGTTTCACTGGCATTGTCGGTATATTTGTCGTAACGCACACCTGGACCGAAtt > 1:222905/1‑67 (MQ=255) tAGCCCAGCGGCTGGGTTTCACTGGCATTGTCGGTATATTTGTCGTAACGCACACCTGGACCGAAtt > 1:1050531/1‑67 (MQ=255) tAGCCCAGCGGCTGGGTTTCACTGGCATTGTCGGTATATTTGTCGTAACGCACACCTGGACCGAAtt > 1:1239111/1‑67 (MQ=255) tAGCCCAGCGGCTGGGTTTCACTGGCATTGTCGGTATATTTGTCGTAACGCACACCTGGACCGAAtt > 1:886095/1‑67 (MQ=255) tAGCCCAGCGGCTGGGTTTCACTGGCATTGTCGGTATATTTGTCGTAACGCACACCTGGACCGAAtt > 1:1417625/1‑67 (MQ=255) tAGCCCAGCGGCTGGGTTTCACTGGCATTGTCGGTATATTTGTCGTAACGCACACCTGGACCGAAtt > 1:86204/1‑67 (MQ=255) gCCCAGCGGCTGGGTTTCTCTGGCATTGTCGGTATATTTGTCGTAACGCACACCAGGGCCGAATTCaaa > 1:1040855/1‑69 (MQ=255) gCCCAGCGGCTGGGTTTCACTGGCATTGTCGGTATATTTGTCGTAACGCACACCAGGGCCGAAtt > 1:2893748/1‑65 (MQ=255) gCCCAGCGGCTGGGTTTCACTGGCATTGTCGGTATATTTGTCGTAACGCACACCAGGGCCGAATTCaaa > 1:3043715/1‑69 (MQ=255) gCCCAGCGGCTGGGTTTCACTGGCATTGTCGGTATATTTGTCGTAACGCACACCAGGGCCGAATTCaaa > 1:637578/1‑69 (MQ=255) gCCCAGCGGCTGGGTTTCACTGGCATTGTCGGTATATTTGTCGTAACGCACACCAGGGCCGAATTCaaa > 1:2304343/1‑69 (MQ=255) gCCCAGCGGCTGGGTTTCACTGGCATTGTCGGTATATTTGTCGTAACGCACACCAGGGCCGAATTCaaa > 1:719299/1‑69 (MQ=255) gCCCAGCGGCTGGGTTTCACTGGCATTGTCGGTATATTTGTCGTAACGCACACCAGGGCCGAATTCaaa > 1:729922/1‑69 (MQ=255) gCCCAGCGGCTGGGTTTCACTGGCATTGTCGGTATATTTGTCGTAACGCACACCAGGGCCGAATTCaaa > 1:2660972/1‑69 (MQ=255) gCCCAGCGGCTGGGTTTCACTGGCATTGTCGGTATATTTGTCGTAACGCACACCAGGGCCGAATTCaa > 1:3409404/1‑68 (MQ=255) cTGGGTTTCACTGGCATTGTCGGTATATTTGTCGTAACGCACACCTGGACCGAATTCAAAGCGGAAACt < 1:124779/69‑1 (MQ=255) cTGGGTTTCACTGGCATTGTCGGTATATTTGTCGTAACGCACACCTGGACCGAATTCAAAGCGGAAACt < 1:3094716/69‑1 (MQ=255) tGGCATTGTCGGTATATTTGTCGTAACGCACACCTGGACCGAATTCAAAGCGGAAACTGTGTACCGGGc < 1:2826845/69‑1 (MQ=255) tGGCATTGTCGGTATATTTGTCGTAACGCACACCTGGACCGAATTCAAAGCGGAAACTGTGTACCGGGc < 1:1962125/69‑1 (MQ=255) tGGCATTGTCGGTATATTTGTCGTAACGCACACCTGGACCGAATTCAAAGCCGAAACTGTGTACCGGGc < 1:104007/69‑1 (MQ=255) tGGCATTGTCGGTATATTTGTCGTAACGCACACCAGGGCCGAATTCAAAGCGGAAACtg < 1:3273388/59‑1 (MQ=255) tGGCATTGTCGGTATATTTGTCGTAACGCACACCAGGGCCGAATTCAAAGCGGAAACtg < 1:939876/59‑1 (MQ=255) tGGCATTGTCGGTATATTTGTCGTAACGCACACCAGGGCCGAATTCAAAGCGGAAACtg < 1:1755535/59‑1 (MQ=255) tGGCATTGTCGGTATATTTGTCGTAACGCACACCAGGGCCGAATTCAAAGCGGAAACtg < 1:1765243/59‑1 (MQ=255) tGGCATTGTCGGTATATTTGTCGTAACGCACACCAGGGCCGAATTCAAAGCGGAAACtg < 1:1814854/59‑1 (MQ=255) tGGCATTGTCGGTATATTTGTCGTAACGCACACCAGGGCCGAATTCAAAGCGGAAACtg < 1:1855939/59‑1 (MQ=255) tGGCATTGTCGGTATATTTGTCGTAACGCACACCAGGGCCGAATTCAAAGCGGAAACtg < 1:3368258/59‑1 (MQ=255) tGGCATTGTCGGTATATTTGTCGTAACGCACACCAGGGCCGAATTCAAAGCGGAAACtg < 1:3317818/59‑1 (MQ=255) tGGCATTGTCGGTATATTTGTCGTAACGCACACCAGGGCCGAATTCAAAGCGGAAACtg < 1:3304121/59‑1 (MQ=255) tGGCATTGTCGGTATATTTGTCGTAACGCACACCAGGGCCGAATTCAAAGCGGAAACtg < 1:2241177/59‑1 (MQ=255) tGGCATTGTCGGTATATTTGTCGTAACGCACACCAGGGCCGAATTCAAAGCGGAAACtg < 1:3231964/59‑1 (MQ=255) tGGCATTGTCGGTATATTTGTCGTAACGCACACCAGGGCCGAATTCAAAGCGGAAACtg < 1:1861641/59‑1 (MQ=255) tGGCATTGTCGGTATATTTGTCGTAACGCACACCAGGGCCGAATTCAAAGCGGAAACtg < 1:1907490/59‑1 (MQ=255) tGGCATTGTCGGTATATTTGTCGTAACGCACACCAGGGCCGAATTCAAAGCGGAAACtg < 1:2431375/59‑1 (MQ=255) tGGCATTGTCGGTATATTTGTCGTAACGCACACCAGGGCCGAATTCAAAGCGGAAACtg < 1:2073342/59‑1 (MQ=255) ggCATTGTCGGTATATTTGTCGTAACGCACACCTGGACCGAATTCAAAGCGGAAACTGTGTACCGGGcc < 1:2461208/69‑1 (MQ=255) ggCATTGTCGGTATATTTGTCGTAACGCACACCAGGGCCGAATTCAAAGCGGAAACtg < 1:2452268/58‑1 (MQ=255) tttGTCGTAACGCACACCTGGACCGAATTCAAAGCGGAAACTGTGTACCGGGCCATTCAAAAACTgacg > 1:664826/1‑69 (MQ=255) tttGTCGTAACGCACACCTGGACCGAATTCAAAGCGGAAACTGTGTACCGGGCCATTCAAAAACTgacg > 1:771338/1‑69 (MQ=255) tttGTCGTAACCCACACCTGGACCGAATTCAAAGCGGAAACTGTGAACCGGGCCATTCAAAAACTgacg > 1:2735237/1‑69 (MQ=255) tGTCGTAACGCACACCTGGACCGAATTCAAAGCGGAAACTGTGTACCGGGCCATTCAAAAACTgacgac > 1:625559/1‑69 (MQ=255) tGTCGTAACGCACACCTGGACCGAATTCAAAGCGGAAACTGTGTACCGGGCCATTCAAAAACTgacga > 1:3102491/1‑68 (MQ=255) gTAACGCACACCTGGACCGAATTCAAAGCGGAAACTGTGTACCGGGCCATTCAAAAACTGACGACCAt > 1:534818/1‑68 (MQ=255) gTAACGCACACCTGGACCGAATTCAAAGCGGAAACTGTGTACCGGGCCATTCAAAAACTGACGACCAt > 1:2493834/1‑68 (MQ=255) | ATAGCCCAGCGGCTGGGTTTCACTGGCATTGTCGGTATATTTGTCGTAACGCACACCTGGACCGAATTCAAAGCGGAAACTGTGTACCGGGCCATTCAAAAACTGACGACCAT > minE/1133250‑1133362 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |