| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 95,017 | T→C | 8.3% | L667L (TTG→CTG) | secA → | preprotein translocase subunit, ATPase that targets protein precursors to the SecYE core translocon |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 95,017 | 0 | T | C | 8.3% | 343.1 / 10.7 | 165 | L667L (TTG→CTG) | secA | preprotein translocase subunit, ATPase that targets protein precursors to the SecYE core translocon |
| Reads supporting (aligned to +/- strand): ref base T (108/43); new base C (4/10); total (112/53) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.94e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.95e-01 | |||||||||||
Only 100 of 165 total aligned reads displayed.
GGAATATGATGACGTGGCTAACGATCAGCGTCGCGCCATTTACTCCCAGCGTAACGAACTGTTGGATGTCAGCGATGTGAGCGAAACCATTAACAGCATTCGTGAAGATGTGTTCAAAGCGACCATTG > minE/94956‑95083 | ggAATATGATGACGTGGCTAACGATCAGCGTCGCGCCATTTACTCCCAGCGTAACGAACTGTTGGAt < 1:517849/67‑1 (MQ=255) gatgaCGTGGCTAACGATCAGCGTCGCGCCATTTACTCCCAGCGTAACGAACTGTTGGATGTCAGCg > 1:1714837/1‑67 (MQ=255) gatgaCGTGGCTAACGATCAGCGTCGCGCCATTTACTCCCAGCGTAACGAACTGTTGGATGTCAGCGAt > 1:2291133/1‑69 (MQ=255) gatgaCGTGGCTAACGATCAGCGTCGCGCCATTTACTCCCAGCGTAACGAACTGTTGGATGTCAGCGAt > 1:2840572/1‑69 (MQ=255) gatgaCGTGGCTAACGATCAGCGTCGCGCCATTTACTCCCAGCGTAACGAACTGTTGGATGTCAGCGAt > 1:2523682/1‑69 (MQ=255) gatgaCGTGGCTAACGATCAGCGTCGCGCCATTTACTCCCAGCGTAACGAACTGTTGGATGTCAGCGAt > 1:1307087/1‑69 (MQ=255) atgaCGTGGCTAACGATCAGCGTCGCGCCATTTACTCCCAGCGTAACGAACTGTTGGATGTCAGCGAt > 1:1402329/1‑68 (MQ=255) atgaCGTGGCTAACGATCAGCGTCGCGCCATTTACTCCCAGCGTAACGAACTGTTGGATGTCAGCGAt > 1:3091363/1‑68 (MQ=255) atgaCGTGGCTAACGATCAGCGTCGCGCCATTTACTCCCAGCGTAACGAACTGTTGGATGTCAGCGAt > 1:1933632/1‑68 (MQ=255) atgaCGTGGCTAACGATCAGCGTCGCGCCATTTACTCCCAGCGTAACGAACTGTTGGATGTCAGCGAt > 1:3249596/1‑68 (MQ=255) atgaCGTGGCTAACGATCAGCGTCGCGCCATTTACTCCCAGCGTAACGAACTGTTGGATGTCAGCGAt > 1:1548513/1‑68 (MQ=255) atgaCGTGGCTAACGATCAGCGTCGCGCCATTTACTCCCAGCGTAACGAACTGTTGGATGTCAGCGAt > 1:266749/1‑68 (MQ=255) atgaCGTGGCTAACGATCAGCGTCGCGCCATTTACTCCCAGCGTAACGAACTGTTGGATGTCAGCGAt > 1:2561963/1‑68 (MQ=255) atgaCGTGGCTAACGATCAGCGTCGCGCCATTTACTCCCAGCGTAACGAACTGTTGGATGTCAGCGAt > 1:1419185/1‑68 (MQ=255) atgaCGTGGCTAACGATCAGCGTCGCGCCATTTACTCCCAGCGTAACGAACTGTTGGATGTCAGCGAt > 1:1379924/1‑68 (MQ=255) atgaCGTGGCTAACGATCAGCGTCGCGCCATTTACTCCCAGCGTAACGAACTGTTGGATGTCAGCGAt > 1:2478626/1‑68 (MQ=255) atgaCGTGGCTAACGATCAGCGTCGCGCCATTTACTCCCAGCGTAACGAACTGTTGGATGTCAGCGAt > 1:2173761/1‑68 (MQ=255) atgaCGTGGCTAACGATCAGCGTCGCGCCATTTACTCCCAGCGTAACGAACTGTTGGATGTCAGCGAt > 1:1085846/1‑68 (MQ=255) atgaCGTGGCTAACGATCAGCGTCGCGCCATTTACTCCCAGCGTAACGAACTGTTGGATGTCAGCGAt > 1:2387039/1‑68 (MQ=255) atgaCGTGGCTAACGATCAGCGTCGCGCCATTTACTCCCAGAGTAACGAACTGTTGGATGTCAGCGAt > 1:2173663/1‑68 (MQ=255) gaCGTGGCTAACGATCAGCGTCGCGCCATTTACTCCCAGCGTAACGAACTGTTGGATGTCAGCGAtgtg < 1:1352258/69‑1 (MQ=255) gaCGTGGCTAACGATCAGCGTCGCGCCATTTACTCCCAGCGTAACGAACTGTTGGATGTCAGCGAtgtg < 1:1288808/69‑1 (MQ=255) gaCGTGGCTAACGATCAGCGTCGCGCCATTTACTCCCAGCGTAACGAACTGCTGGATGTCAGCGAtgtg < 1:2656835/69‑1 (MQ=255) gaCGTGGCTAACGATCAGCGTCGCGCCATTTACTCCCAGCGTAACGAACTGCTGGATGTCAGCGAtgtg < 1:33798/69‑1 (MQ=255) gaCGTGGCTAACGATCAGCGTCGCGCCATTTACTCCCAGCGTAACGAACTGCTGGATGTCAGCGAtgtg < 1:1893742/69‑1 (MQ=255) gaCGTGGCTAACGATCAGCGTCGCGCCATTTACTCCCAGCGTAACGAACTGCTGGATGTCAGCGAtgtg < 1:917574/69‑1 (MQ=255) ggCTAACGATCAGCGTCGCGCCATTTACTCCCAGCGTAACGAACTGCTGGATGTCAGCGATGTGAGCGa > 1:3218013/1‑69 (MQ=255) ggCTAACGATCAGCGTCGCGCCATTTACTCCCAGCGTAACGAACTGCTGGATGTCAGCGATGTGAGCGa > 1:560996/1‑69 (MQ=255) cAGCGTCGCGCCATTTACTCCCAGCGTAACGAACTGTTGGATGTCAGCGATGTGAGCGAAACCATTAAc > 1:3132873/1‑69 (MQ=255) aTTTACTCCCAGCGTAACGAACTGTTGGATGTCAGCGATGTGAGCGAAACCATTAACAGCATTCGTGaa < 1:2876889/69‑1 (MQ=255) aTTTACTCCCAGCGTAACGAACTGTTGGATGTCAGCGATGTGAGCGAAACCATTAACAGCATTCGTGaa < 1:2398167/69‑1 (MQ=255) aTTTACTCCCAGCGTAACGAACTGTTGGATGTCAGCGATGTGAGCGAAACCATTAACAGCATTCGTGaa < 1:366659/69‑1 (MQ=255) aTTTACTCCCAGCGTAACGAACTGTTGGATGTCAGCGATGTGAGCGAAACCATTAACAGCATTCGTGaa < 1:642498/69‑1 (MQ=255) tttACTCCCAGCGTAACGAACTGTTGGATGTCAGCGATGTGAGCGAAACCATTAACAGCATTCGTGaa < 1:708378/68‑1 (MQ=255) tACTCCGAGCGTAACGAACTGTTGGATGTCAGCGATGTGAGCGAAACCAtt > 1:4691/1‑51 (MQ=255) tACTCCCAGCGTAACGTACTGTTGGATGTCAGCGATGTGAGCGAAACCAtt < 1:457840/51‑1 (MQ=255) tACTCCCAGCGTAACGCACTGTTGGATGTCAGCGATGTGa > 1:1776655/1‑40 (MQ=255) tACTCCCAGCGTAACGAACTGTTGGATGTCAGCGATGTGAGCGAAACCAtt < 1:2588183/51‑1 (MQ=255) tACTCCCAGCGTAACGAACTGTTGGATGTCAGCGATGTGAGCGAAACCAtt > 1:236151/1‑51 (MQ=255) tACTCCCAGCGTAACGAACTGTTGGATGTCAGCGATGTGAGCGAAACCAtt > 1:855489/1‑51 (MQ=255) tACTCCCAGCGTAACGAACTGTTGGATGTCAGCGATGTGAGCGAAACCAtt < 1:833053/51‑1 (MQ=255) tACTCCCAGCGTAACGAACTGTTGGATGTCAGCGATGTGAGCGAAACCAtt < 1:795471/51‑1 (MQ=255) tACTCCCAGCGTAACGAACTGTTGGATGTCAGCGATGTGAGCGAAACCAtt > 1:3221540/1‑51 (MQ=255) tACTCCCAGCGTAACGAACTGTTGGATGTCAGCGATGTGAGCGAAACCAtt > 1:968083/1‑51 (MQ=255) tACTCCCAGCGTAACGAACTGTTGGATGTCAGCGATGTGAGCGAAACCAtt > 1:2958286/1‑51 (MQ=255) tACTCCCAGCGTAACGAACTGTTGGATGTCAGCGATGTGAGCGAAACCAtt > 1:3002872/1‑51 (MQ=255) tACTCCCAGCGTAACGAACTGTTGGATGTCAGCGATGTGAGCGAAACCAtt < 1:2273428/51‑1 (MQ=255) tACTCCCAGCGTAACGAACTGTTGGATGTCAGCGATGTGAGCGAAACCAtt > 1:1252371/1‑51 (MQ=255) tACTCCCAGCGTAACGAACTGTTGGATGTCAGCGATGTGAGCGAAACCAtt > 1:1160992/1‑51 (MQ=255) tACTCCCAGCGTAACGAACTGTTGGATGTCAGCGATGTGAGCGAAACCAtt > 1:1962659/1‑51 (MQ=255) tACTCCCAGCGTAACGAACTGTTGGATGTCAGCGATGTGAGCGAAACCAtt > 1:1107182/1‑51 (MQ=255) tACTCCCAGCGTAACGAACTGTTGGATGTCAGCGATGTGAGCGAAACCATTAACAGc > 1:1606509/1‑57 (MQ=255) tACTCCCAGCGTAACGAACTGTTGGATGTCAGCGATGTGAGCGAAACCATTAACAGCATTCGTGAAGa > 1:2696430/1‑68 (MQ=255) tACTCCCAGCGTAACGAACTGTTGGATGTCAGCGATGTGAGCGAAACCATTAACAGCATTCGTGAAGAt > 1:3315418/1‑69 (MQ=255) tACTCCCAGCGTAACGAACTGTTGGATGTCAGCGATGTGAGCGAAACCATTAACAGCATTCGTGAAGAt > 1:566676/1‑69 (MQ=255) tACTCCCAGCGTAACGAACTGTTGGATGTCAGCGATGTGAGCGAAACCATTAACAGCATTCGTGAAGAt > 1:1118014/1‑69 (MQ=255) tACTCCCAGCGTAACGAACTGTTGGATGTCAGCGATGTGAGCGAAACCATTAACAGCATTCGTGAAGAt > 1:2555184/1‑69 (MQ=255) cccAGCGTAACGAACTGTTGGATGTCAGCGATGTGAGCGAAACCATTAACAGCATTCGTGAAGAtgtg < 1:1424058/68‑1 (MQ=255) gCGTAACGAACTGTTGGATGTCAGCGATGTGAGCGAAACCATTAACAGCATTCGTGAAGATGTGTTCaa < 1:1523000/69‑1 (MQ=255) tAACGAACTGTTGGATGTCAGCGATGTGAGCGAAACCATTAACAGCATTCGTGAAGATGTGTTCAAAGc < 1:740580/69‑1 (MQ=255) tAACGAACTGTTGGATGTCAGCGATGTGAGCGAAACCATTAACAGCATTCGTGAAGATGTGTTCAAAGc < 1:2951124/69‑1 (MQ=255) aaCGAACTGTTGGATGTCAGCGATGTGAGCGAAACCATTAACAGCATTCGTGAAGATGTGTTCAAAGCg < 1:2164326/69‑1 (MQ=255) aaCGAACTGTTGGATGTCAGCGATGTGAGCGAAACCATTAACAGCATTCGTGAAGATGTGTTCAAAGCg < 1:1768827/69‑1 (MQ=255) aCGAACTGTTGGATGTCAGCGATGTGAGCGAACCCATTAACAGCATTCGTGAAGATGTGTTCAAAGCg > 1:2544120/1‑68 (MQ=255) aCGAACTGTTGGATGTCAGCGATGTGAGCGAAACCATTaa > 1:2709286/1‑40 (MQ=255) aCGAACTGTTGGATGTCAGCGATGTGAGCGAAACCATTAACAGCATTCGTGAAGATGTGTTCAAAgag > 1:3085176/1‑66 (MQ=255) aCGAACTGTTGGATGTCAGCGATGTGAGCGAAACCATTAACAGCATTCGTGAAGATGTGTTCAAAGc > 1:2115112/1‑67 (MQ=255) aCGAACTGTTGGATGTCAGCGATGTGAGCGAAACCATTAACAGCATTCGTGAAGATGTGTTCAAAGc > 1:1049372/1‑67 (MQ=255) aCGAACTGTTGGATGTCAGCGATGTGAGCGAAACCATTAACAGCATTCGTGAAGATGTGTTCAAAGCg > 1:1004293/1‑68 (MQ=255) aCGAACTGTTGGATGTCAGCGATGTGAGCGAAACCATTAACAGCATTCGTGAAGATGTGTTCAAAGCg > 1:1051386/1‑68 (MQ=255) aCGAACTGTTGGATGTCAGCGATGTGAGCGAAACCATTAACAGCATTCGTGAAGATGTGTTCAAAGCg > 1:2344599/1‑68 (MQ=255) aCGAACTGTTGGATGTCAGCGATGTGAGCGAAACCATTAACAGCATTCGTGAAGATGTGTTCAAAGCg > 1:3435620/1‑68 (MQ=255) aCGAACTGTTGGATGTCAGCGATGTGAGCGAAACCATTAACAGCATTCGTGAAGATGTGTTCAAAGCg > 1:34890/1‑68 (MQ=255) aCGAACTGTTGGATGTCAGCGATGTGAGCGAAACCATTAACAGCATTCGTGAAGATGTGTTCAAAGCg > 1:151648/1‑68 (MQ=255) aCGAACTGTTGGATGTCAGCGATGTGAGCGAAACCATTAACAGCATTCGTGAAGATGTGTTCAAAGCg > 1:417320/1‑68 (MQ=255) aCGAACTGTTGGATGTCAGCGATGTGAGCGAAACCATTAACAGCATTCGTGAAGATGTGTTCAAAGCg > 1:1454885/1‑68 (MQ=255) aCGAACTGTTGGATGTCAGCGATGTGAGCGAAACCATTAACAGCATTCGTGAAGATGTGTTCAAAGCg > 1:103939/1‑68 (MQ=255) aCGAACTGTTGGATGTCAGCGATGTGAGCGAAACCATTAACAGCATTCGTGAAGATGTGTTCAAAGCg > 1:632238/1‑68 (MQ=255) aCGAACTGTTGGATGTCAGCGATGTGAGCGAAACCATTAACAGCATTCGTGAAGATGTGTTCAAAGCg > 1:1936462/1‑68 (MQ=255) aCGAACTGTTGGATGTCAGCGATGTGAGCGAAACCATTAACAGCATTCGTGAAGATGTGTTCAAAGCg > 1:2339902/1‑68 (MQ=255) aCGAACTGTTGGATGTCAGCGATGTGAGCGAAACCATTAACAGCATTCGTGAAGATGTGTTCAAAGCg > 1:2445322/1‑68 (MQ=255) aCGAACTGTTGGATGTCAGCGATGTGAGCGAAACCATTAACAGCATTCGTGAAGATGTGTTCAAAGCg > 1:2075602/1‑68 (MQ=255) aCGAACTGTTGGATGTCAGCGATGTGAGCGAAACCATTAACAGCATTCGTGAAGATGTGTTCAAAGCg > 1:2651490/1‑68 (MQ=255) aCGAACTGTTGGATGTCAGCGATGTGAGCGAAACCATTAACAGCATTCGTGAAGATGTGTTCAAAGCg > 1:19868/1‑68 (MQ=255) aCGAACTGTTGGATGTCAGCGATGTGAGCGAAACCATTAACAGCATTCGTGAAGATGTGTTCAAAGCg > 1:1939783/1‑68 (MQ=255) aCGAACTGTTGGATGTCAGCGATGTGAGCGAAACCATTAACAGCATTCGTGAAGATGTGTTCAAAGCg > 1:2845752/1‑68 (MQ=255) aCGAACTGTTGGATGTCAGCGATGTGAGCGAAACCATTAACAGCATTCGTGAAGATGTGTTCAAAGCg > 1:3286142/1‑68 (MQ=255) aCGAACTGTTGGATGTCAGCGATGTGAGCGAAACCATTAACAGCATTCGTGAAGATGTGTTCAAAGCg > 1:2937391/1‑68 (MQ=255) aCGAACTGTTGGATGTCAGCGATGTGAGCGAAACCATTAACAGCATTCGTGAAGATGTGTTCAAAGCg > 1:1877088/1‑68 (MQ=255) aCGAACTGTTGGATGTCAGCGATGTGAGCGAAACCATTAACAGCATTCGTGAAGATGTGTTCAAAGCg > 1:1871825/1‑68 (MQ=255) aCGAACTGTTGGATGTCAGCGATGTGAGCGAAACCATTAACAGCATTCGTGAAGATGTGTTCAAAGCg > 1:3031224/1‑68 (MQ=255) aCGAACTGTTGGATGTCAGCGATGTGAGCGAAACCATTAACAGCATTCGTGAAGATGTGTTCAAAGCg > 1:1808957/1‑68 (MQ=255) aCGAACTGTTGGATGTCAGCGATGTGAGCGAAACCATTAACAGCATTCGTGAAGATGTGTTCAAAGCg > 1:1631623/1‑68 (MQ=255) aCGAACTGTTGGATGTCAGCCATGTGAGCGAAACCATTAACAg > 1:804171/1‑43 (MQ=255) gAACTGTTGGATGTCAGCGATGTGAGCGAAACCATTAACAGCATTCGTGAAGATGTGTTCAAAGCGAc < 1:358663/68‑1 (MQ=255) cTGTTGGATGTCAGCGATGTGAGCGAAACCATTAACAGCATTCGTGAAGATGTGTTCAAAGCGACCAtt < 1:3012897/69‑1 (MQ=255) tGTTGGATGTCAGCGATGTGAGCGAAACCATTAACAGCATTCGTGAAGATGTGTTCAAAGCGACCATTg < 1:1463445/69‑1 (MQ=255) tGTTGGATGTCAGCGATGTGAGCGAAACCATTAACAGCATTCGTGAAGATGTGTTCAAAGCGACCATTg < 1:275475/69‑1 (MQ=255) tGTTGGATGTCAGCGATGTGAGCGAAACCATTAACAGCATTCGTGAAGATGTGTTCAAAGCGACCATTg < 1:2036025/69‑1 (MQ=255) tGTTGGATGTCAGCGATGTGAGCGAAACCATTAACAGCATTCGTGAAGATGTGTTCAAAGCGACCATTg < 1:1565775/69‑1 (MQ=255) | GGAATATGATGACGTGGCTAACGATCAGCGTCGCGCCATTTACTCCCAGCGTAACGAACTGTTGGATGTCAGCGATGTGAGCGAAACCATTAACAGCATTCGTGAAGATGTGTTCAAAGCGACCATTG > minE/94956‑95083 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |