Predicted mutation
evidence seq id position mutation freq annotation gene description
RA minE 1,134,888 T→C 40.6% V276V (GTT→GTC)  pfkB → 6‑phosphofructokinase II

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE1,134,8880TC40.6% 35.1 / 64.6 91V276V (GTT→GTC) pfkB6‑phosphofructokinase II
Reads supporting (aligned to +/- strand):  ref base T (14/40);  new base C (22/15);  total (36/55)
Fisher's exact test for biased strand distribution p-value = 2.09e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.12e-02

GCATGGTCGGCGCGATGACACTGAAACTGGCAGAAAATGCCTCTCTTGAAGAGATGGTTCGTTTTGGCGTAGCTGCGGGGAGTGCAGCCACACTCAATCAGGGAACACGTCTGTGCTCCCAT  >  minE/1134830‑1134951
                                                          |                                                               
gCATGGTCGGCGCGATGACACTGAAACTGGCAGAAAATGCCTCTCTTGAAGAGATGGTTCGTTTTGGCg                                                       <  1:3374952/69‑1 (MQ=255)
gCATGGTCGGCGCGATGACACTGAAACTGGCAGAAAATGCCTCTCTTGAAGAGATGGTTCGTTTTGGCg                                                       <  1:1190402/69‑1 (MQ=255)
gCATGGTCGGCGCGATGACACTGAAACTGGCAGAAAATGCCTCTCTTGAAGAGATGGTTCGTTTTGGCg                                                       <  1:1676882/69‑1 (MQ=255)
gCATGGTCGGCGCGATGACACTGAAACTGGCAGAAAATGCCTCTCTTGAAGAGATGGTTCGTTTTGGCg                                                       <  1:1732995/69‑1 (MQ=255)
gCATGGTCGGCGCGATGACACTGAAACTGGCAGAAAATGCCTCTCTTGAAGAGATGGTTCGTTTTGGCg                                                       <  1:2059465/69‑1 (MQ=255)
 cATGGTCGGCGCGATGACACTGAAACTGGCAGAAAATGCCTCTCTTGAAGAGATGGTTCGTTTTGGCGt                                                      <  1:965343/69‑1 (MQ=255)
 cATGGTCGGCGCGATGACACTGAAACTGGCAGAAAATGCCTCTCTTGAAGAGATGGTTCGTTTTGGCGt                                                      <  1:800241/69‑1 (MQ=255)
 cATGGTCGGCGCGATGACACTGAAACTGGCAGAAAATGCCTCTCTTGAAGAGATGGTTCGTTTTGGCGt                                                      <  1:864940/69‑1 (MQ=255)
 cATGGTCGGCGCGATGACACTGAAACTGGCAGAAAATGCCTCTCTTGAAGAGATGGTTCGTTTTGGCGt                                                      <  1:1664327/69‑1 (MQ=255)
 cATGGTCGGCGCGATGACACTGAAACTGGCAGAAAATGCCTCTCTTGAAGAGATGGTTCGTTTTGGCGt                                                      <  1:1503592/69‑1 (MQ=255)
 cATGGTCGGCGCGATGACACTGAAACTGGCAGAAAATGCCTCTCTTGAAGAGATGGTTCGTTTTGGCGt                                                      <  1:1932710/69‑1 (MQ=255)
 cATGGTCGGCGCGATGACACTGAAACTGGCAGAAAATGCCTCTCTTGAAGAGATGGTTCGTTTTGGCGt                                                      <  1:3205669/69‑1 (MQ=255)
       cGGCGCTATGACACTTAAACTGGCAGAAAATGCCTCTCTTGAAGAGATGGTCCGTTTTGGCGTAGCTGc                                                <  1:673467/69‑1 (MQ=255)
       cGGCGCGATGACACTTAAACTGGCAGAAAATTCCTCTCTTGAAGAGATGGTCCGTTTTGGCGTAGCTGc                                                <  1:946416/69‑1 (MQ=255)
       cGGCGCGATGACACTTAAACTGGCAGAAAATGCCTCTCTTGAAGAGATGGTCCGTTTTGGCGTAGCTGc                                                <  1:57079/69‑1 (MQ=255)
       cGGCGCGATGACACTTAAACTGGCAGAAAATGCCTCTCTTGAAGAGATGGTCCGTTTTGGCGTAGCTGc                                                <  1:2141953/69‑1 (MQ=255)
       cGGCGCGATGACACTTAAACTGGCAGAAAATGCCTCTCTTGAAGAGATGGTCCGTTTTGGCGTAGCTGc                                                <  1:2537665/69‑1 (MQ=255)
       cGGCGCGATGACACTTAAACTGGCAGAAAATGCCTCTCTTGAAGAGATGGTCCGTTTTGGCGTAGCTGc                                                <  1:2980194/69‑1 (MQ=255)
       cGGCGCGATGACACTTAAACTGGCAGAAAATGCCTCTCTTGAAGAGATGGTCCGTTTTGGCGTAGCTGc                                                <  1:3157269/69‑1 (MQ=255)
       cGGCGCGATGACACTTAAACTGGCAGAAAATGCCTCTCTTGAAGAGATGGTCCGTTTTGGCGTAGCTGc                                                <  1:2116402/69‑1 (MQ=255)
       cGGCGCGATGACACTTAAACTGGCAGAAAATGCCTCTCTTGAAGAGATGGTCCGTTTTGGCGTAGCTGc                                                <  1:1228901/69‑1 (MQ=255)
       cGGCGCGATGACACTTAAACTGGCAGAAAATGCCTCTCTTGAAGAGATGGTCCGTTTTGGCGTAGCTGc                                                <  1:1517651/69‑1 (MQ=255)
       cGGCGCGATGACACTTAAACTGGCAGAAAATGCCTCTCTTGAAGAGATGGTCCGTTTTGGCGTAGCTGc                                                <  1:1119784/69‑1 (MQ=255)
       cGGCGCGATGACACTTAAACTGGCAGAAAATGCCTCTCTTGAAGAGATGGTCCGTTTTGGCGTAGCTGc                                                <  1:1424284/69‑1 (MQ=255)
       cGGCGCGATGACACCTAAACTGGCAGAAAATGCCTCTCTTGAAGAGATGGTCCGTTTTGGCGTAGCTGc                                                <  1:1474269/69‑1 (MQ=255)
       cGGCGCGATGAAACTTAAACTGGCAGAAAATGCCTCTCTTGAAGAGATGGTCCGTTTTGGCGTAGCTGc                                                <  1:1965446/69‑1 (MQ=255)
        ggCGCGATGACACTTAAACTGGCAGAAAATGCCTCTCTTGAAGAGATGGTCCGTTTTGGCGTAGCTGc                                                <  1:415268/68‑1 (MQ=255)
                   acTTAAACTGGCAGAAAATGCCTCTCTTGAAGAGATGGTCCGTTTTGGCGTAGCTGCGGGGAGagcagc                                    >  1:3051312/1‑69 (MQ=255)
                   acTTAAACTGGCAGAAAATGCCTCTCTTGAAGAGATGGTCCGTTTTGGCGTAGCTGCGGGGAGTGCAGc                                    >  1:2506630/1‑69 (MQ=255)
                   acTTAAACTGGCAGAAAATGCCTCTCTTGAAGAGATGGTCCGTTTTGGCGTAGCTGCGGGGAGTGCAGc                                    >  1:2278955/1‑69 (MQ=255)
                   acTTAAACTGGCAGAAAATGCCTCTCTTGAAGAGATGGTCCGTTTTGGCGTAGCTGCGGGGAGTGCAGc                                    >  1:2280585/1‑69 (MQ=255)
                   acTTAAACTGGCAGAAAATGCCTCTCTTGAAGAGATGGTCCGTTTTGGCGTAGCTGCGGGGAGTGCAGc                                    >  1:2435953/1‑69 (MQ=255)
                   acTTAAACTGGCAGAAAATGCCTCTCTTGAAGAGATGGTCCGTTTTGGCGTAGCTGCGGGGAGTGCAGc                                    >  1:494193/1‑69 (MQ=255)
                   acTTAAACTGGCAGAAAATGCCTCTCTTGAAGAGATGGTCCGTTTTGGCGTAGCTGCGGGGAGTGCAGc                                    >  1:2746254/1‑69 (MQ=255)
                   acTTAAACTGGCAGAAAATGCCTCTCTTGAAGAGATGGTCCGTTTTGGCGTAGCTGCGGGGAGTGCAGc                                    >  1:457460/1‑69 (MQ=255)
                   acTTAAACTGGCAGAAAATGCCTCTCTTGAAGAGATGGTCCGTTTTGGCGTAGCTGCGGGGAGTGCAGc                                    >  1:1276333/1‑69 (MQ=255)
                   acTTAAACTGGCAGAAAATGCCTCTCTTGAAGAGATGGTCCGTTTTGGCGTAGCTGCGGGGAGTGCAGc                                    >  1:277105/1‑69 (MQ=255)
                   acTTAAACTGGCAGAAAATGCCTCTCTTGAAGAGATGGTCCGTTTTGGCGTAGCTGCGGGGAGTGCAGc                                    >  1:2128571/1‑69 (MQ=255)
                   acTTAAACTGGCAGAAAATGCCTCTCTTGAAGAGATGGTCCGTTTTGGCGTAGCTGCGGGGAGTGCAGc                                    >  1:3356277/1‑69 (MQ=255)
                   acTTAAACTGGCAGAAAATGCCTCTCTTGAAGAGATGGTCCGTTTTGGCGTAGCTGCGGGGAGTGCAGc                                    >  1:1781491/1‑69 (MQ=255)
                   acTTAAACTGGCAGAAAATGCCTCTCTTGAAGAGATGGTCCGTTTTGGCGTAGCTGCGGGGAGTGCAGc                                    >  1:1791778/1‑69 (MQ=255)
                   acTTAAACTGGCAGAAAATGCCTCTCTTGAAGAGATGGTCCGTTTTGGCGTAGCTGCGGGGAGTGCAGc                                    >  1:1469823/1‑69 (MQ=255)
                   acTTAAACTGGCAGAAAATGCCTCTCTTGAAGAGATGGTCCGTTTTGGCGTAGCTGCGGGGAGTGCAGc                                    >  1:1441551/1‑69 (MQ=255)
                   acTTAAACTGGCAGAAAATGCCTCTCTTGAAGAGATGGTCCGTTTTGGCGTAGCTGCGGGGAGTGCAGc                                    >  1:3427802/1‑69 (MQ=255)
                   acTTAAACTGGCAGAAAATGCCTCTCTTGAAGAGATGGTCCGTTTTGGCGTAGCTGCGGGGAGTGCAGc                                    >  1:1207678/1‑69 (MQ=255)
                   acTTAAACTGGCAGAAAATGCCTCTCTTGAAGAGATGGTCCGTTTTGGCGTAGCTGCGGGGAGTGCAGc                                    >  1:3178560/1‑69 (MQ=255)
                   acTTAAACTGGCAGAAAATGCCTCTCTTGAAGAGATGGTCCGTTTTGGCGTAGCTGCGGGGAGTGCAGc                                    >  1:1285642/1‑69 (MQ=255)
                   acTTAAACTGGCAGAAAATGCCTCTCTTGAAAAGATGGTCCGt                                                              >  1:3168690/1‑43 (MQ=37)
                   acTTAAACTGGCAGAAAATGCCTCTCTT‑AAGAGATGGTCCGTTTTGGCGTAGCTGCGGGGAGTGCAGc                                    >  1:2452090/1‑68 (MQ=255)
                      gAAACTGGCAGAAAATGCCTCTCTTGAAGAGGTGGTTCGTTTTGGCGTAGCTGCgggg                                            >  1:718935/1‑58 (MQ=255)
                      gAAACTGGCAGAAAATGCCTCTCTTGAAGAGATGGTTCGTTTTGGCGTAGCTGCgggg                                            >  1:3088137/1‑58 (MQ=255)
                      gAAACTGGCAGAAAATGCCTCTCTTGAAGAGATGGTTCGTTTTGGCGTAGCTGCgggg                                            >  1:120885/1‑58 (MQ=255)
                      gAAACTGGCAGAAAATGCCTCTCTTGAAGAGATGGTTCGTTTTGGCGTAGCTGCgggg                                            >  1:2983361/1‑58 (MQ=255)
                      gAAACTGGCAGAAAATGCCTCTCTTGAAGAGATGGTTCGTTTTGGCGTAGCTGCgggg                                            >  1:531145/1‑58 (MQ=255)
                      gAAACTGGCAGAAAATGCCTCTCTTGAAGAGATGGTTCGTTTTGGCGTAGCTGCgggg                                            >  1:491337/1‑58 (MQ=255)
                      gAAACTGGCAGAAAATGCCTCTCTTGAAGAGATGGTTCGTTTTGGCGTAGCTGCgggg                                            >  1:2607699/1‑58 (MQ=255)
                      gAAACTGGCAGAAAATGCCTCTCTTGAAGAGATGGTTCGTTTTGGCGTAGCTGCgggg                                            >  1:2606490/1‑58 (MQ=255)
                      gAAACTGGCAGAAAATGCCTCTCTTGAAGAGATGGTTCGTTTTGGCGTAGCTGCgggg                                            >  1:2533518/1‑58 (MQ=255)
                      gAAACTGGCAGAAAATGCCTCTCTTGAAGAGATGGTTCGTTTTGGCGTAGCTGCgggg                                            >  1:233560/1‑58 (MQ=255)
                      gAAACTGGCAGAAAATGCCTCTCTTGAAGAGATGGTTCGTTTTGGCGTAGCTGCgggg                                            >  1:2226233/1‑58 (MQ=255)
                      gAAACTGGCAGAAAATGCCTCTCTTGAAGAGATGGTTCGTTTTGGCGTAGCTGCgggg                                            >  1:13223/1‑58 (MQ=255)
                        aaCTGGCAGTAAATGCCTCTCTTGAAGAGATGGTTCGTTTTGGCGt                                                      <  1:76117/46‑1 (MQ=255)
                                  aaaTGCCTCTCTTGAAGAGATGGTTCGTTTTGGCGTAGCTGCGGGGAGTGCAGCCACACTCAATCAggg                     >  1:148575/1‑69 (MQ=255)
                                  aaaTGCCTCTCTTGAAGAGATGGTTCGTTTTGGCGTAGCTGCGGGGAGTGCAGCCACACTCAATCAggg                     >  1:1629943/1‑69 (MQ=255)
                                               gAAGAGATGGTTCGTTTTGGCGTAGCTGCGGGGAGTGCAGGCACACTCAATCAGGGAACACGTCtg           <  1:309365/66‑1 (MQ=255)
                                               gAAGAGATGGTTCGTTTTGGCGTAGCTGCGGGGAGTGCAGCCACACTCAATCAGGGAACACGTCtg           <  1:516652/66‑1 (MQ=255)
                                               gAAGAGATGGTTCGTTTTGGCGTAGCTGCGGGGAGTGCAGCCACACTCAATCAGGGAACACGTCtg           <  1:534394/66‑1 (MQ=255)
                                               gAAGAGATGGTTCGTTTTGGCGTAGCTGCGGGGAGTGCAGCCACACTCAATCAGGGAACACGTCtg           <  1:1088780/66‑1 (MQ=255)
                                               gAAGAGATGGTTCGTTTTGGCGTAGCTGCGGGGAGTGCAGCCACACTCAATCAGGGAACACGTCtg           <  1:652642/66‑1 (MQ=255)
                                               gAAGAGATGGTTCGTTTTGGCGTAGCTGCGGGGAGTGCAGCCACACTCAATCAGGGAACACGTCtg           <  1:1546377/66‑1 (MQ=255)
                                               gAAGAGATGGTTCGTTTTGGCGTAGCTGCGGGGAGTGCAGCCACACTCAATCAGGGAACACGTCtg           <  1:3259856/66‑1 (MQ=255)
                                               gAAGAGATGGTTCGTTTTGGCGTAGCTGCGGGGAGTGCAGCCACACTCAATCAGGGAACACGTCtg           <  1:2087265/66‑1 (MQ=255)
                                               gAAGAGATGGTTCGTTTTGGCGTAGCTGCGGGGAGTGCAGCCACACTCAATCAGGGAACACGTCtg           <  1:2431450/66‑1 (MQ=255)
                                               gAAGAGATGGTTCGTTTTGGCGTAGCTGCGGGGAGTGCAGCCACACTCAATCAGGGAACACGTCtg           <  1:3171414/66‑1 (MQ=255)
                                               gAAGAGATGGTTCGTTTTGGCGTAGCTGCGGGGAGTGCAGCCACACTCAATCAGGGAACACGTCtg           <  1:3016281/66‑1 (MQ=255)
                                               gAAGAGATGGTTCGTTTTGGCGTAGCTGCGGGGAGTGCAGCCACACTCAATCAGGCAACACGTCtg           <  1:2469919/66‑1 (MQ=255)
                                                aaGTGATGGTTCGTTTTGGCGTAGCTGCGGGGAGTGCAGCCACACTCAATCAGGGAACACGTCtg           <  1:3378984/65‑1 (MQ=255)
                                                    gaTGGTTCGTTTTGGCGTAGCTGCGGGGAGTGCAGCCACACTCAATCAGGGAACACGTCtg           <  1:15178/61‑1 (MQ=255)
                                                     aTGGTTCGTTTTGGCGTAGCTGCGGGGAGTGCAGCCACACTCAATCAGGGAACACGTCTGTGCTCCCAt  <  1:2476560/69‑1 (MQ=255)
                                                     aTGGTTCGTTTTGGCGTAGCTGCGGGGAGTGCAGCCACACTCAATCAGGGAACACGTCTGTGCTCCCAt  <  1:2472731/69‑1 (MQ=255)
                                                     aTGGTTCGTTTTGGCGTAGCTGCGGGGAGTGCAGCCACACTCAATCAGGGAACACGTCTGTGCTCCCAt  <  1:45504/69‑1 (MQ=255)
                                                     aTGGTTCGTTTTGGCGTAGCTGCGGGGAGTGCAGCCACACTCAATCAGGGAACACGTCTGTGCTCCCAt  <  1:405485/69‑1 (MQ=255)
                                                     aTGGTTCGTTTTGGCGTAGCTGCGGGGAGTGCAGCCACACTCAATCAGGGAACACGTCTGTGCTCCCAt  <  1:1925629/69‑1 (MQ=255)
                                                     aTGGTTCGTTTTGGCGTAGCTGCGGGGAGTGCAGCCACACTCAATCAGGGAACACGTCTGTGCTCCCAt  <  1:1638400/69‑1 (MQ=255)
                                                     aTGGTTCGTTTTGGCGTAGCTGCGGGGAGTGCAGCCACACTCAATCAGGGAACACGTCTGTGCTCCCAt  <  1:1574311/69‑1 (MQ=255)
                                                     aTGGTTCGTTTTGGCGTAGCTGCGGGGAGTGCAGCCACACTCAATCAGGGAACACGTCTGTGCTCCCAt  <  1:3266520/69‑1 (MQ=255)
                                                     aTGGTTCGTTTTGGCGTAGCTGCGGGGAGTGCAGCCACACTCAATCAGGGAACACGTCTGTGCTCCCAt  <  1:1417829/69‑1 (MQ=255)
                                                     aTGGTTCGTTTTGGCGTAGCTGCGGGGAGTGCAGCCACACTCAATCAGGGAACACGTCTGTGCTCCCAt  <  1:1011620/69‑1 (MQ=255)
                                                      tGGTTCGTTTTGGCGTAGCTGCGGGGAGTGCAGCCACACTCAATCAGGGAACACGTCTGTGCTCCCAt  <  1:2688317/68‑1 (MQ=255)
                                                      tGGTTCGTTTTGGCGTAGCTGCGGGGAGTGCAGCCACACTCAATCAGGGAACACGTCTGTGCTCCCAt  <  1:394494/68‑1 (MQ=255)
                                                      tGGTTCGTTTTGGCGTAGCTGCGGGGAGTGCAGCCACACTCAATCAGGGAACACGTCTGTGCTCCCAt  <  1:2062571/68‑1 (MQ=255)
                                                          |                                                               
GCATGGTCGGCGCGATGACACTGAAACTGGCAGAAAATGCCTCTCTTGAAGAGATGGTTCGTTTTGGCGTAGCTGCGGGGAGTGCAGCCACACTCAATCAGGGAACACGTCTGTGCTCCCAT  >  minE/1134830‑1134951

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑