BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	minE	1,134,939	T→C	26.7%	R293R (CGT→CGC)	pfkB →	6‑phosphofructokinase II

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	1,134,939	0	T	C	26.7%	120.3 / 43.2	108	R293R (CGT→CGC)	pfkB	6‑phosphofructokinase II
Reads supporting (aligned to +/- strand): ref base T (42/37); new base C (12/17); total (54/54)
Fisher's exact test for biased strand distribution p-value = 3.85e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.86e-01

Only 100 of 111 total aligned reads displayed.

GAAGAGATGGTTCGTTTTGGCGTAGCTGCGGGGAGTGCAGCCACACTCAATCAGGGAACACGTCTGTGCTCCCATGACGATACGCAAAAAATTTACGCTTACCTTTCCCGCTAACAAAAACATTCCCCCAG  >  minE/1134877‑1135007

gAAGAGATGGTTCGTTTTGGCGTAGCTGCGGGGAGTGCAGGCACACTCAATCAGGGAACACGTCtg                                                                   <  1:309365/66‑1 (MQ=255)

gAAGAGATGGTTCGTTTTGGCGTAGCTGCGGGGAGTGCAGCCACACTCAATCAGGGAACACGTCtg                                                                   <  1:3016281/66‑1 (MQ=255)

gAAGAGATGGTTCGTTTTGGCGTAGCTGCGGGGAGTGCAGCCACACTCAATCAGGGAACACGTCtg                                                                   <  1:1088780/66‑1 (MQ=255)

gAAGAGATGGTTCGTTTTGGCGTAGCTGCGGGGAGTGCAGCCACACTCAATCAGGGAACACGTCtg                                                                   <  1:516652/66‑1 (MQ=255)

gAAGAGATGGTTCGTTTTGGCGTAGCTGCGGGGAGTGCAGCCACACTCAATCAGGGAACACGTCtg                                                                   <  1:652642/66‑1 (MQ=255)

gAAGAGATGGTTCGTTTTGGCGTAGCTGCGGGGAGTGCAGCCACACTCAATCAGGGAACACGTCtg                                                                   <  1:2431450/66‑1 (MQ=255)

gAAGAGATGGTTCGTTTTGGCGTAGCTGCGGGGAGTGCAGCCACACTCAATCAGGGAACACGTCtg                                                                   <  1:3171414/66‑1 (MQ=255)

gAAGAGATGGTTCGTTTTGGCGTAGCTGCGGGGAGTGCAGCCACACTCAATCAGGGAACACGTCtg                                                                   <  1:2087265/66‑1 (MQ=255)

gAAGAGATGGTTCGTTTTGGCGTAGCTGCGGGGAGTGCAGCCACACTCAATCAGGGAACACGTCtg                                                                   <  1:3259856/66‑1 (MQ=255)

gAAGAGATGGTTCGTTTTGGCGTAGCTGCGGGGAGTGCAGCCACACTCAATCAGGGAACACGTCtg                                                                   <  1:1546377/66‑1 (MQ=255)

gAAGAGATGGTTCGTTTTGGCGTAGCTGCGGGGAGTGCAGCCACACTCAATCAGGCAACACGTCtg                                                                   <  1:2469919/66‑1 (MQ=255)

 aaGTGATGGTTCGTTTTGGCGTAGCTGCGGGGAGTGCAGCCACACTCAATCAGGGAACACGTCtg                                                                   <  1:3378984/65‑1 (MQ=255)

     gaTGGTTCGTTTTGGCGTAGCTGCGGGGAGTGCAGCCACACTCAATCAGGGAACACGTCtg                                                                   <  1:15178/61‑1 (MQ=255)

      aTGGTTCGTTTTGGCGTAGCTGCGGGGAGTGCAGCCACACTCAATCAGGGAACACGTCTGTGCTCCCAt                                                          <  1:3266520/69‑1 (MQ=255)

      aTGGTTCGTTTTGGCGTAGCTGCGGGGAGTGCAGCCACACTCAATCAGGGAACACGTCTGTGCTCCCAt                                                          <  1:1011620/69‑1 (MQ=255)

      aTGGTTCGTTTTGGCGTAGCTGCGGGGAGTGCAGCCACACTCAATCAGGGAACACGTCTGTGCTCCCAt                                                          <  1:1925629/69‑1 (MQ=255)

      aTGGTTCGTTTTGGCGTAGCTGCGGGGAGTGCAGCCACACTCAATCAGGGAACACGTCTGTGCTCCCAt                                                          <  1:405485/69‑1 (MQ=255)

      aTGGTTCGTTTTGGCGTAGCTGCGGGGAGTGCAGCCACACTCAATCAGGGAACACGTCTGTGCTCCCAt                                                          <  1:45504/69‑1 (MQ=255)

      aTGGTTCGTTTTGGCGTAGCTGCGGGGAGTGCAGCCACACTCAATCAGGGAACACGTCTGTGCTCCCAt                                                          <  1:2472731/69‑1 (MQ=255)

      aTGGTTCGTTTTGGCGTAGCTGCGGGGAGTGCAGCCACACTCAATCAGGGAACACGTCTGTGCTCCCAt                                                          <  1:2476560/69‑1 (MQ=255)

      aTGGTTCGTTTTGGCGTAGCTGCGGGGAGTGCAGCCACACTCAATCAGGGAACACGTCTGTGCTCCCAt                                                          <  1:1638400/69‑1 (MQ=255)

      aTGGTTCGTTTTGGCGTAGCTGCGGGGAGTGCAGCCACACTCAATCAGGGAACACGTCTGTGCTCCCAt                                                          <  1:1574311/69‑1 (MQ=255)

      aTGGTTCGTTTTGGCGTAGCTGCGGGGAGTGCAGCCACACTCAATCAGGGAACACGTCTGTGCTCCCAt                                                          <  1:1417829/69‑1 (MQ=255)

       tGGTTCGTTTTGGCGTAGCTGCGGGGAGTGCAGCCACACTCAATCAGGGAACACGTCTGTGCTCCCAt                                                          <  1:2688317/68‑1 (MQ=255)

       tGGTTCGTTTTGGCGTAGCTGCGGGGAGTGCAGCCACACTCAATCAGGGAACACGTCTGTGCTCCCAt                                                          <  1:394494/68‑1 (MQ=255)

       tGGTTCGTTTTGGCGTAGCTGCGGGGAGTGCAGCCACACTCAATCAGGGAACACGTCTGTGCTCCCAt                                                          <  1:2062571/68‑1 (MQ=255)

                           gCGGGGAGTGCAGCCACACTCAATCAGGGAACACGTCTGTGCTCCCATGACGATACGCAAAAAATTTAc                                     <  1:1331029/69‑1 (MQ=255)

                           gCGGGGAGTGCAGCCACACTCAATCAGGGAACACGTCTGTGCTCCCATGACGATACGCAAAAAATTTAc                                     <  1:2105545/69‑1 (MQ=255)

                           gCGGGGAGTGCAGCCACACTCAATCAGGGAACACGTCTGTGCTCCCATGACGATACGCAAAAAATTTAc                                     <  1:1528046/69‑1 (MQ=255)

                                 aGTGCAGCCACACTCAATCAGGGAACCCGTCTGTGCTCCCATGACGATACGCAAAAAAt                                         >  1:2401773/1‑59 (MQ=255)

                                 aGTGCAGCCACACTCAATCAGGGAACACGTCTGTTCTCCCATGACGATACGCAAAAAAtt                                        >  1:2274439/1‑60 (MQ=255)

                                 aGTGCAGCCACACTCAATCAGGGAACACGTCTGTGCTCCCATg                                                         >  1:2818462/1‑43 (MQ=255)

                                 aGTGCAGCCACACTCAATCAGGGAACACGTCTGTGCTCCCATGACGATACGCAAAAAAtt                                        >  1:1629012/1‑60 (MQ=255)

                                 aGTGCAGCCACACTCAATCAGGGAACACGTCTGTGCTCCCATGACGATACGCAAAAAAtt                                        >  1:3152480/1‑60 (MQ=255)

                                 aGTGCAGCCACACTCAATCAGGGAACACGTCTGTGCTCCCATGACGATACGCAAAAAAtt                                        >  1:1176754/1‑60 (MQ=255)

                                 aGTGCAGCCACACTCAATCAGGGAACACGTCTGTGCTCCCATGACGATACGCAAAAAAtt                                        >  1:2406000/1‑60 (MQ=255)

                                 aGTGCAGCCACACTCAATCAGGGAACACGTCTGTGCTCCCATGACGATACGCAAAAAAtt                                        >  1:2256606/1‑60 (MQ=255)

                                 aGTGCAGCCACACTCAATCAGGGAACACGTCTGTGCTCCCATGACGATACGCAAAAAAtt                                        >  1:2071074/1‑60 (MQ=255)

                                 aGTGCAGCCACACTCAATCAGGGAACACGTCTGTGCTCCCATGACGATACGCAAAAAAtt                                        >  1:1555209/1‑60 (MQ=255)

                                 aGTGCAGCCACACTCAATCAGGGAACACGTCTGTGCTCCCATGACGATACGCAAAAAAtt                                        >  1:1562478/1‑60 (MQ=255)

                                 aGTGCAGCCACACTCAATCAGGGAACACGTCTGTGCTCCCATGACGATACGCAAAAAATTTACGCTTAc                               <  1:2459310/69‑1 (MQ=255)

                                 aGTGCAGCCACACTCAATCAGGGAACACGTCTGTGCTCCCATGACGATACGCAAAAAATTTACGCTTAc                               <  1:2455424/69‑1 (MQ=255)

                                 aGTGCAGCCACACTCAATCAGGGAACACGTCTGTGCTCCCATGACGATACGCAAAAAATTTACGCTTAc                               <  1:2864480/69‑1 (MQ=255)

                                 aGTGCAGACACACTCAATCAGGAAACACGTCTGTGCTCCCATGACGATACGCAAAAAAtt                                        >  1:1857426/1‑60 (MQ=255)

                                     cAGCCACACTCAATCAGGGCACACGTCTGTGCTCCCATGACGATACGCa                                               >  1:1312604/1‑49 (MQ=255)

                                     cAGCCACACTCAATCAGGGAACACGTCTGTGCTCCCATGACGATACGCAAAAAATTTACGCTTACCttt                           >  1:1705146/1‑69 (MQ=255)

                                     cAGCCACACTCAATCAGGGAACACGTCTGTGCTCCCATGACGATACGCAAAAAATTTACGCTTACCttt                           >  1:1133640/1‑69 (MQ=255)

                                     cAGCCACACTCAATCAGGGAACACGTCTGTGCTCCCATGACGATACGCAAAAAATTTACGCTTACCttt                           >  1:3033584/1‑69 (MQ=255)

                                     cAGCCACACTCAATCAGGGAACACGTCTGTGCTCCCATGACGATACGCAAAAAATTTACGCTTACCttt                           >  1:1630557/1‑69 (MQ=255)

                                     cAGCCACACTCAATCAGGGAACACGTCTGTGCTCCCATGACGATACGCAAAAAATTTACGCTTACCttt                           >  1:2267035/1‑69 (MQ=255)

                                     cAGCCACACTCAATCAGGGAACACGTCTGTGCTCCCATGACGATACGCAAAAAATTTACGCTTACCttt                           >  1:2237425/1‑69 (MQ=255)

                                     cAGCCACACTCAATCAGGGAACACGTCTGTGCTCCCATGACGATACGCAAAAAATTTACGCTTACCttt                           >  1:2205899/1‑69 (MQ=255)

                                     cAGCCACACTCAATCAGGGAACACGTCTGTGCTCCCATGACGATACGCAAAAAATTTACGCTTACCttt                           >  1:2988986/1‑69 (MQ=255)

                                     cAGCCACACTCAATCAGGGAACACGTCTGTGCTCCCATGACGATACGCAAAAAATTTACGCTTACCttt                           >  1:2961855/1‑69 (MQ=255)

                                         cacaCTCAACCAGGGAACACGCCTGTGCTCCCATGACGATACGCAAAAAATTTTCGCGTACCttt                           >  1:2918282/1‑65 (MQ=255)

                                         cacaCTCAACCAGGGAACACGCCTGTGCTCCCATGACGATACGCAAAAAATTTGCGCGTACCttt                           >  1:2922207/1‑65 (MQ=255)

                                         cacaCTCAACCAGGGAACACGCCTGTGCTCCCATGACGATACGCAAAAAATTTACGCGTACCttt                           >  1:2803302/1‑65 (MQ=255)

                                         cacaCTCAACCAGGGAACACGCCTGTGCTCCCATGACGATACGCAAAAAATTTACGCGTACCttt                           >  1:724271/1‑65 (MQ=255)

                                         cacaCTCAACCAGGGAACACGCCTGTGCTCCCATGACGATACGCAAAAAATTTACGCGTACCttt                           >  1:3134046/1‑65 (MQ=255)

                                         cacaCTCAACCAGGGAACACGCCTGTGCTCCCATGACGATACGCAAAAAATTTACGCGTACCttt                           >  1:2624846/1‑65 (MQ=255)

                                         cacaCTCAACCAGGGAACACGCCTGTGCTCCCATGACGATACGCAAAAAATTTACGCGTACCttt                           >  1:1314452/1‑65 (MQ=255)

                                         cacaCTCAACCAGGGAACACGCCTGTGCTCCCATGACGATACGCAAAAAATTTACGCGTACCttt                           >  1:1943008/1‑65 (MQ=255)

                                         cacaCTCAACCAGGGAACACGCCTGTGCTCCCATGACGATACGCAAAAAATTTACGCGTACCttt                           >  1:3183944/1‑65 (MQ=255)

                                         cacaCTCAACCAGGGAACACGCCTGTGCTCCCATGACGATACGCAAAAAATTTACGCGTACCttt                           >  1:1535980/1‑65 (MQ=255)

                                         cacaCTCAACCAGGGAACACGCCTGTGCTCCCATGACGATACGCAAAAAATTTAAGCGTACCttt                           >  1:343078/1‑65 (MQ=255)

                                             cTCAATCAGGGAACACGTCTGTGCTCCCATGACGATACGCAAAAAATTTACGCTTACCTTTCCCGCTa                    >  1:408335/1‑68 (MQ=255)

                                             cTCAATCAGGGAACACGTCTGTGCTCCCATGACGATACGCAAAAAATTTACGCTTACCTTTCCCGCTa                    >  1:718639/1‑68 (MQ=255)

                                             cTCAATCAGGGAACACGTCTGTGCTCCCATGACGATACGCAAAAAATTTACGCTTACCTTTCCCGCTa                    >  1:1024998/1‑68 (MQ=255)

                                             cTCAATCAGGGAACACGTCTGTGCTCCCATGACGATACGCAAAAAATTTACGCTTACCTTTCCCGCTa                    >  1:2817065/1‑68 (MQ=255)

                                             cTCAATCAGGGAACACGTCTGTGCTCCCATGACGATACGCAAAAAATTTACGCTTACCTTTCCCGCTa                    >  1:1783615/1‑68 (MQ=255)

                                             cTCAATCAGGGAACACGTCTGTGCTCCCATGACGATACGCAAAAAATTTACGCTTACCTTTCCCGCTa                    >  1:1788760/1‑68 (MQ=255)

                                             cTCAATCAGGGAACACGTCTGTGCTCCCATGACGATACGCAAAAAATTTACGCTAACCTTTCCCGCTa                    >  1:2805931/1‑68 (MQ=255)

                                               cAACCAGGGAACACGCCTGTGCTCCCTTGACGATACGCAAAAAATTTACGCGTACCTTTc                          <  1:3004209/60‑1 (MQ=255)

                                               cAACCAGGGAACACGCCTGTGCTCCCATGACGATACGCAAAAAATTTACGCGTACCTTTc                          <  1:1938212/60‑1 (MQ=255)

                                               cAACCAGGGAACACGCCTGTGCTCCCATGACGATACGCAAAAAATTTACGCGTACCTTTc                          <  1:850778/60‑1 (MQ=255)

                                               cAACCAGGGAACACGCCTGTGCTCCCATGACGATACGCAAAAAATTTACGCGTACCTTTc                          <  1:1260874/60‑1 (MQ=255)

                                               cAACCAGGGAACACGCCTGTGCTCCCATGACGATACGCAAAAAATTTACGCGTACCTTTc                          <  1:1735143/60‑1 (MQ=255)

                                               cAACCAGGGAACACGCCTGTGCTCCCATGACGATACGCAAAAAATTTACGCGTACCTTTc                          <  1:2923810/60‑1 (MQ=255)

                                               cAACCAGGGAACACGCCTGTGCTCCCATGACGATACGCAAAAAATTTACGCGTACCTTTc                          <  1:1997102/60‑1 (MQ=255)

                                               cAACCAGGGAACACGCCTGTGCTCCCATGACGATACGCAAAAAATTTACGCGTACCTTTc                          <  1:3211976/60‑1 (MQ=255)

                                               cAACCAGGGAACACGCCTGTGCTCCCATGACGATACGCAAAAAATTTACGCGTACCTTTc                          <  1:2140151/60‑1 (MQ=255)

                                               cAACCAGGGAACACGCCTGTGCTCCCATGACGATACGCAAAAAATTTACGCGTACCTTTc                          <  1:2648244/60‑1 (MQ=255)

                                               cAACCAGGGAACACGCCTGTGCTCCCATCACGATACGCAAAAAATTTACGCGTACCTTTc                          <  1:1974878/60‑1 (MQ=255)

                                                 accAGGGAACACGCCTGTGCTCCCATGACGATACGCAAAAAATTTACGCGTACCTTTc                          <  1:666530/56‑1 (MQ=255)

                                                       gAACACGTCTGTGCTCCCATGACGATACGCAAAAAATTTACGCTTACCTTTCCCGCTAACAAAAACAtt         >  1:2856886/1‑69 (MQ=255)

                                                       gAACACGTCTGTGCTCCCATGACGATACGCAAAAAATTTACGCTTACCTTTCCCGCTAACAAAAACAtt         >  1:1565913/1‑69 (MQ=255)

                                                       gAACACGTCTGTGCTCCCATGACGATACGCAAAAAATTTACGCTTACCTTTCCCGCTAACAAAAACAtt         >  1:1574566/1‑69 (MQ=255)

                                                          cacGTCTGTGCTCCCATGACGATACGCAAAAAATTTACGCTTACCTTTCCCGCTAACaaaaa             <  1:3164834/62‑1 (MQ=255)

                                                          cacGTCTGTGCTCCCATGACGATACGCAAAAAATTTACGCTTACCTTTCCCGCTAACaaaaa             <  1:2542910/62‑1 (MQ=255)

                                                           acGTCTGTGCTCCCATGACGATACGCAAAAAATTTACGCTTACCTTTCCCGCTAACaaaaa             <  1:1951688/61‑1 (MQ=255)

                                                           acGTCTGTGCTCCCATGACGATACGCAAAAAATTTACGCTTACCTTTCCCGCTAACaaaaa             <  1:943410/61‑1 (MQ=255)

                                                             gTCTGTGCTCCCATGACGATACGCAAAAAATTTACGCTTACCTTTCCCGCTAACAAAAACATTccccc    >  1:502518/1‑68 (MQ=255)

                                                             gTCTGTGCTCCCATGACGATACGCAAAAAATTTACGCTTACCTTTCCCGCTAACAAAAACATTccccc    >  1:62828/1‑68 (MQ=255)

                                                             gTCTGTGCTCCCATGACGATACGCAAAAAATTTACGCTTACCTTTCCCGCTAACAAAAACATTccccc    >  1:1621577/1‑68 (MQ=255)

                                                             gTCTGTGCTCCCATGACGATACGCAAAAAATTTACGCTTACCTTTCCCGCTAACAAAAACATTccccc    >  1:2058164/1‑68 (MQ=255)

                                                             gTCTGTGCTCCCATGACGATACGCAAAAAATTTACGCTTACCTTTCCCGCTAACAAAAACATTccccc    >  1:435521/1‑68 (MQ=255)

                                                             gTCTGTGCTCCCATGACGATACGCAAAAAATTTACGCTTACCTTTCCCGCTAACAAAAACATTccccc    >  1:2273479/1‑68 (MQ=255)

                                                              tCTGTGCTCCCATGACGATACGCAAAAAATTTACGCTTACCTTTCCCGCTAACAAAAACATTCCCCCAg  <  1:929635/69‑1 (MQ=255)

                                                              tCTGTGCTCCCATGACGATACGCAAAAAATTTACGCTTACCTTTCCCGCTAACAAAAACATTCCCCCAg  <  1:3433634/69‑1 (MQ=255)

                                                              tCTGTGCTCCCATGACGATACGCAAAAAATTTACGCTTACCTTTCCCGCTAACAAAAACATTCCCCCAg  <  1:945744/69‑1 (MQ=255)

GAAGAGATGGTTCGTTTTGGCGTAGCTGCGGGGAGTGCAGCCACACTCAATCAGGGAACACGTCTGTGCTCCCATGACGATACGCAAAAAATTTACGCTTACCTTTCCCGCTAACAAAAACATTCCCCCAG  >  minE/1134877‑1135007

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`