| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,135,605 | A→G | 28.9% | H40R (CAT→CGT) | yniA → | predicted phosphotransferase/kinase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,135,605 | 0 | A | G | 28.9% | 72.5 / 33.1 | 76 | H40R (CAT→CGT) | yniA | predicted phosphotransferase/kinase |
| Reads supporting (aligned to +/- strand): ref base A (45/9); new base G (12/10); total (57/19) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.73e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GCGTAATGAACTGCCTGGCGGAGAAGTCCATGCCGCATGGCATTTGCGCTATGCAGGACATGATTTTTTCGTCAAATGTGATGAAAGGGAACTGCTTCCCGGTTTTACCGCCGAAGCCGACCAAC > minE/1135546‑1135670 | gCGTAATGAACTGCCTGGCGGAGAAGTCCATGCCGCATGGCATTTGCGCTATGCAGGACGTGActtttt > 1:917187/1‑69 (MQ=255)gCGTAATGAACTGCCTGGCGGAGAAGTCCATGCCGCATGGCATTTGCGCTATGCAGGACGTGActtttt > 1:2271148/1‑69 (MQ=255)gCGTAATGAACTGCCTGGCGGAGAAGTCCATGCCGCATGGCATTTGCGCTATGCAGGACGTGActtttt > 1:2356534/1‑69 (MQ=255)gCGTAATGAACTGCCTGGCGGAGAAGTCCATGCCGCATGGCATTTGCGCTATGCAGGACGTGActtttt > 1:2564526/1‑69 (MQ=255)gCGTAATGAACTGCCTGGCGGAGAAGTCCATGCCGCATGGCATTTGCGCTATGCAGGACGTGActtttt > 1:2642964/1‑69 (MQ=255)gCGTAATGAACTGCCTGGCGGAGAAGTCCATGCCGCATGGCATTTGCGCTATGCAGGACGTGActtttt > 1:2900390/1‑69 (MQ=255)gCGTAATGAACTGCCTGGCGGAGAAGTCCATGCCGCATGGCATTTGCGCTATGCAGGACGTGActtttt > 1:3036280/1‑69 (MQ=255)gCGTAATGAACTGCCTGGCGGAGAAGTCCATGCCGCATGGCATTTGCGCTATGCAGGACGTGActtttt > 1:380447/1‑69 (MQ=255)gCGTAATGAACTGCCTGGCGGAGAAGTCCATGCCGCATGGCATTTGCGCTATGCAGGACGTGActtttt > 1:1505013/1‑69 (MQ=255)gCGTAATGAACTGCCTGGCGGAGAAGTCCATGCCGCATGGCATTTGCGCTATGCAGGACGTGActtttt > 1:620147/1‑69 (MQ=255)gCGTAATGAACTGCCTGGCGGAGAAGTCCATGCCGCATGGCATTTGCGCTATGCAGGACGTGActttt > 1:2191622/1‑68 (MQ=255)gCGAAATGAACTGCCAGCCGGACAAGTCCAAGCCGCATGGCATTTGCCCTATGCAGGACGTGac > 1:2267227/1‑63 (MQ=255) tAATGAACTGCCTGGCGGAGAAGTCCATGCCGCATGGCATTTGCGCTATGCAGGACATGATTTTTTc < 1:416106/67‑1 (MQ=255) tAATGAACTGCCTGGCGGAGAAGTCCATGCCGCATGGCATTTGCGCTATGCAGGACATGATTTTTTc < 1:628818/67‑1 (MQ=255) aTGAACTGCCTGGCGGAGAAGTCCATGCCGCATGGCATTTGCGCTATGCAGGACATGATTTTTTCGTCa > 1:51233/1‑69 (MQ=255) aTGAACTGCCTGGCGGAGAAGTCCATGCCGCATGGCATTTGCGCTATGCAGGACATGATTTTTTCGTCa > 1:1661540/1‑69 (MQ=255) aTGAACTGCCTGGCGGAGAAGTCCATGCCGCATGGCATTTGCGCTATGCAGGACATGATTTTTTCGTCa > 1:3012271/1‑69 (MQ=255) aTGAACTGCCTGGCGGAGAAGTCCATGCCGCATGGCATTTGCGCTATGCAGGACATGATTTTTTCGTCa > 1:519737/1‑69 (MQ=255) aTGAACTGCCTGGCGGAGAAGTCCATGCCGCATGGCATTTGCGCTATGCAGGACATGATTTTTTCGTCa > 1:2682628/1‑69 (MQ=255) aTGAACTGCCTGGCGGAGAAGTCCATGCCGCATGGCATTTGCGCTATGCAGGACATGATTTTTTCGTCa > 1:11866/1‑69 (MQ=255) cTGCCTGGCGGAGAAGTCCATGCCGCATGGCATTTGCGCTATGCAGGACATGATTTTTTCGTCAAAtgt < 1:1902996/69‑1 (MQ=255) gagaAGTCCATGCCGCATGGCATTTGCGCTATGCAGGACATGATTTTTTCGTCAAATGTGATGAAAggg > 1:763161/1‑69 (MQ=255) gagaAGTCCATGCCGCATGGCATTTGCGCTATGCAGGACATGATTTTTTCGTCAAATGTGATGAAAggg > 1:1256235/1‑69 (MQ=255) gagaAGTCCATGCCGCATGGCATTTGCGCTATGCAGGACATGATTTTTTCGTCAAATGTGATGAAAggg > 1:2023253/1‑69 (MQ=255) gagaAGTCCATGCCGCATGGCATTTGCGCTATGCAGGACATGATTTTTTCGTCAAATGTGATGAAAggg > 1:2724539/1‑69 (MQ=255) gagaAGTCCATGCCGCATGGCATTTGCGCTATGCAGGACATGATTTTTTCGTCAAATGTGATGAAAggg > 1:3409389/1‑69 (MQ=255) gagaAGTCCATGCCGCATGGCATTTGCGCTATGCAGGACATGATTTTTTCGTCAAATGTGATGAAAg > 1:1119952/1‑67 (MQ=255) tGGCATTTGCGCTATGCAGGACATGATTTTTTCGTCAAATGTGATGAAAGGGAACTGCTTCCCGGtttt < 1:698706/69‑1 (MQ=255) tGGCATTTGCGCTATGCAGGACATGATTTTTTCGTCAAATGTGATGAAAGGGAACTGCTTCCCGGtttt < 1:2371893/69‑1 (MQ=255) tGGCATTTGCGCTATGCAGGACATGATTTTTTCGTCAAATGTGATGAAAGGGAACTGCTTCCCGGtttt > 1:1661484/1‑69 (MQ=255) tGGCATTTGCGCTATGCAGGACATGATTTTTTCGTCAAATGTGATGAAAGGGAACTGCTTCCCGGtttt < 1:895528/69‑1 (MQ=255) tGGCATTTGCGCTATGCAGGACATGATTTTTTCGTCAAATGTGATGAAAGGGAACTGCTTCCCGGtttt > 1:2808530/1‑69 (MQ=255) tGGCATTTGCGCTATGCAGGACATGATTTTTTCGTCAAATGTGATGAAAGGGAACTGCTTCCCGGtttt < 1:2532767/69‑1 (MQ=255) tGGCATTTGCGCTATGCAGGACATGATTTTTTCGTCAAATGTGATGAAAGGGAACTGCTTCCCGGtttt < 1:2728667/69‑1 (MQ=255) tGGCATTTGCGCTATGCAGGACATGATTTTTTCGTCAAATGTGATGAAAGGGAACTGCTTCCCGGtttt > 1:1711427/1‑69 (MQ=255) tGGCATTTGCGCTATGCAGGACATGATTTTTTCGTCAAATGTGATGAAAAGGAACTGCTTCCCGGtttt < 1:195293/69‑1 (MQ=255) tGGCATTTGCGCTATGCAGGACATGATTTTTTCGTAAAATGTGATGAAAGGGAACTGCTTCCCGGtttt > 1:2138835/1‑69 (MQ=255) gCATTTGCGCTATGCAGGACATGATTTTTTCGTCAAATGTGATGAAAGGGAACTGCTTc > 1:2037528/1‑59 (MQ=255) gCATTTGCGCTATGCAGGACATGATTTTTTCGTCAAATGTGATGAAAGGGAACTGCTTc > 1:2591521/1‑59 (MQ=255) gCATTTGCGCTATGCAGGACATGATTTTTTCGTCAAATGTGATGAAAGGGAACTGCTTc > 1:1078972/1‑59 (MQ=255) gCATTTGCGCTATGCAGGACATGATTTTTTCGTCAAATGTGATGAAAGGGAACTGCTTc > 1:2499015/1‑59 (MQ=255) gCATTTGCGCTATGCAGGACATGATTTTTTCGTCAAATGTGATGAAAGGGAACTGCTTc > 1:1686203/1‑59 (MQ=255) gCATTTGCGCTATGCAGGACATGATTTTTTCGTCAAATGTGATGAAAGGGAACTGCTTc > 1:2877966/1‑59 (MQ=255) gCATTTGCGCTATGCAGGACATGATTTTTTCGTCAAATGTGATGAAAGGGAACTGCTTc > 1:614019/1‑59 (MQ=255) gCATTTGCGCTATGCAGGACATGATTTTTTCGTCAAATGTGATGAAAGGGAACTGCTTc > 1:2935166/1‑59 (MQ=255) gCATTTGCGCTATGCAGGACATGATTTTTTCGTCAAATGTGATGAAAGGGAACTGCTTc > 1:1341129/1‑59 (MQ=255) gCATTTGCGCTATGCAGGACATGATTTTTTCGTCAAATGTGATGAAAGGGAACTGCTTc > 1:3028816/1‑59 (MQ=255) gCATTTGCGCTATGCAGGACATGATTTTTTCGTCAAATGTGATGAAAGGGAACTGCTTCCCGGTTTTAc > 1:1646751/1‑69 (MQ=255) gCATTTGCGCTATGCAGGACATGATTTTTTCGTCAAATGTGATGAAAGGGAACTGCTTCCCGGTTTTAc > 1:135070/1‑69 (MQ=255) gCATTTGCGCTATGCAGGACATGATTTTTTCGTCAAATGTGATGAAAGGGAACTGCTTCCCGGTTTTAc > 1:1871984/1‑69 (MQ=255) gCATTTGCGCTATGCAGGACATGATTTTTTCGTCAAATGTGATGAAAGGGAACTGCTTCCCGGTTTTAc > 1:1967232/1‑69 (MQ=255) gCATTTGCGCTATGCAGGACATGATTTTTTCGTCAAATGTGATGAAAGGGAACTGCTTCCCGGTTTTAc > 1:2406747/1‑69 (MQ=255) gCATTTGCGCTATGCAGGACATGATTTTTTCGTCAAATGTGATGAAAGGGAACTGCTTCCAGGTTTTAc > 1:1531159/1‑69 (MQ=255) tGCGCTATGCAGGACATGATTTTTTCGTCAAATGTGATGAAAGGGAACTGCTTCCCGGTTTTAccgc > 1:1318512/1‑67 (MQ=255) tGCGCTATGCAGGACATGATTTTTTCGTCAAATGTGATGAAAGGGAACTGCTTCCCGGTTTTAccg > 1:405604/1‑66 (MQ=255) tGCAGGACATGATTTTTTCGTCAAATGTGATGAAAGGGAACTGCTTCCCGGTTTTAccg > 1:1761094/1‑59 (MQ=255) tGCAGGACATGATTTTTTCGTCAAATGTGATGAAAGGGAACTGCTTCCCGGTTTTACCGCCGAAGcc > 1:863714/1‑67 (MQ=255) tGCAGGACATGATTTTTTCGTCAAATGTGATGAAAGGGAACTGCTTCCCGGTTTTACCGCCGAAGcc > 1:2341113/1‑67 (MQ=255) tGCAGGACATGATTTTTTCGTCAAATGTGATGAAAGGGAACTGCTTCCCGGTTTTACCGCCGAAGcc > 1:973063/1‑67 (MQ=255) tGCAGGACATGATTTTTTCGTCAAATGTGATGAAAGGGAACTGCTTCCCGGTTTTACCGCCGAAGcc > 1:367947/1‑67 (MQ=255) tGCAGGACATGATTTTTTCGTCAAATGTGATGAAAGGGAACTGCTTCCCGGTTTTACCGCCGAAGcc > 1:316424/1‑67 (MQ=255) tGCAGGACATGATTTTTTCGTCAAATGTGATGAAAGGGAACTGCTTCCCGGTTTTACCGCCGAAGcc > 1:2988537/1‑67 (MQ=255) cAGGACGTGACTTTTTCGTCAAATGTGATGAAAGGGAACTGCt < 1:726717/43‑1 (MQ=255) cAGGACGTGACTTTTTCGTCAAATGTGATGAAAGGGAACTGCt < 1:2428326/43‑1 (MQ=255) cAGGACGTGACTTTTTCGTCAAATGTGATGAAAGGGAACTGCt < 1:876642/43‑1 (MQ=255) cAGGACGTGACTTTTTCGTCAAATGTGATGAAAGGGAACTGCt < 1:873648/43‑1 (MQ=255) cAGGACGTGACTTTTTCGTCAAATGTGATGAAAGGGAACTGCt < 1:848388/43‑1 (MQ=255) cAGGACGTGACTTTTTCGTCAAATGTGATGAAAGGGAACTGCt < 1:1787746/43‑1 (MQ=255) cAGGACGTGACTTTTTCGTCAAATGTGATGAAAGGGAACTGCt < 1:30401/43‑1 (MQ=255) cAGGACGTGACTTTTTCGTCAAATGTGATGAAAGGGAACTGCt < 1:3227701/43‑1 (MQ=255) cAGGACGTGACTTTTTCGTCAAATGTGATGAAAGGGAACTGCt < 1:334798/43‑1 (MQ=255) cAGGACATGATTTTTTCGTCAAATGTGATGAAAGGGAACTGCTTCCCGGTTTTACCGCCGAAGCCGAcc > 1:645440/1‑69 (MQ=255) cAGGACATGATTTTTTCGTCAAATGTGATGAAAGGGAACTGCTTCCCGGTTTTACCGCCGAAGCCGAcc > 1:282799/1‑69 (MQ=255) cAGGACATGATTTTTTCGTCAAATGTGATGAAAGGGAACTGCTTCCCGGTTTTACCGCCGAAGCCGAcc > 1:2048665/1‑69 (MQ=255) gACGTGACTTTTTCGTCAAATGTGATGAAAGGGAACTGCt < 1:490855/40‑1 (MQ=255) gACATGATTTTTTCGTCAAATGTGATGAAAGGGAACTGCTTCCCGGTTTTACCGCCGAAGCCGACCAAc > 1:484641/1‑69 (MQ=255) | GCGTAATGAACTGCCTGGCGGAGAAGTCCATGCCGCATGGCATTTGCGCTATGCAGGACATGATTTTTTCGTCAAATGTGATGAAAGGGAACTGCTTCCCGGTTTTACCGCCGAAGCCGACCAAC > minE/1135546‑1135670 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |