Predicted mutation
evidence seq id position mutation freq annotation gene description
RA minE 1,137,810 T→C 31.9% intergenic (+71/‑92) yniC → / → ydjM predicted hydrolase/predicted inner membrane protein regulated by LexA

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE1,137,8100TC31.9% 56.4 / 35.9 72intergenic (+71/‑92)yniC/ydjMpredicted hydrolase/predicted inner membrane protein regulated by LexA
Reads supporting (aligned to +/- strand):  ref base T (11/38);  new base C (21/2);  total (32/40)
Fisher's exact test for biased strand distribution p-value = 4.93e-08
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.44e-01

CAGGGGCAGTGATCTCGCTGCCCCTGGTTCTTTATCTGAAATTGCATTCAACTGACGGATTAATCGTCAATTTAAGAGAAAGAGTTACACCGTCACCACTTCCGTGCACTGTATAAAAATCCTATACTGTACGT  >  minE/1137744‑1137877
                                                                  |                                                                   
cAGGGGCAGTGATCTCGCTGCCCCTGGTTCTTTATCTGAAATTGCATTCAACTGACGGATTAATCGTc                                                                    <  1:302913/68‑1 (MQ=255)
cAGGGGCAGTGATCTCGCTGCCCCTGGTTCTTTATCTGAAATTGCATTCAACTGACGGATTAATCGTc                                                                    <  1:2299303/68‑1 (MQ=255)
cAGGGGCAGTGATCTCGCTGCCCCTGGTTCTTTATCTGAAATTGCATTCAACTGACGGATTAATCGTc                                                                    <  1:2194778/68‑1 (MQ=255)
     gCAGTGATCTCGCTGCCCCTGGTTCTTTATCTGAAATTGCATTCAACTGACGGATTAATCGTCaa                                                                  >  1:1484198/1‑65 (MQ=255)
     gCAGTGATCTCGCTGCCCCTGGTTCTTTATCTGAAATTGCATTCAACTGACGGATTAATCGTCaa                                                                  >  1:785328/1‑65 (MQ=255)
     gCAGTGATCTCGCTGCCCCTGGTTCTTTATCTGAAATTGCATTCAACTGACGGATTAATCGTCAAt                                                                 >  1:541418/1‑66 (MQ=255)
          gATCTCGCTGCCCCTGGTTCTTTATCTGAAATTGCATTCAACTGACGGATTAATCGTCAATTTAAgaga                                                         <  1:3232657/69‑1 (MQ=255)
           aTCTCGCTGCCCCTGGTTCTTTATCTGAAATTGCATTCAAGTGACGGATTAATCGTCAATTTAAgaga                                                         <  1:2845125/68‑1 (MQ=255)
                acTGCCCCTGGTTCTTTATCTGAATTTGCATTCAACTGACGGATTAATCGCCAATTTAAGAGAAAGAGt                                                   <  1:2757166/68‑1 (MQ=255)
                acTGCCCCTGGTTCTTTATCTGAATTTGCATTCAACTGACGGATTAATCGCCAATTTAAGAGAAAGAGt                                                   <  1:646008/68‑1 (MQ=255)
                         ggTTCTTTATCTGAAATTGCATTCAACTGACGGATTAATCGTCAATTTAAGAGAAAGAGTTACACCGTc                                          >  1:3182761/1‑69 (MQ=255)
                          gTTCTTTATCTGAAATTGCATTCAACTGACGGATTAATCGTCAATTTAAGAGAAAGAGTTACACCGTc                                          >  1:1417597/1‑68 (MQ=255)
                          gTTCTTTATCTGAAATTGCATTCAACTGACGGATTAATCGTCAATTTAAGAGAAAGAGTTACACCGTc                                          >  1:3001166/1‑68 (MQ=255)
                          gTTCTTTATCTGAAATTGCATTCAACTGACGGATTAATCGTCAATTTAAGAGAAAGAGTTACACCGTc                                          >  1:2350757/1‑68 (MQ=255)
                          gTTCTTTATCTGAAATTGCATTCAACTGACGGATTAATCGTCAATTTAAGAGAAAGAGTTACACCGTc                                          >  1:2294206/1‑68 (MQ=255)
                             cTTTATCTGAAATTGCATTCAACTGACGGATTACTCGTCAATTTaa                                                             <  1:479532/46‑1 (MQ=255)
                             cTTTATCTGAAATTGCATTCAACTGACGGATTAATCGTCAATTTaa                                                             <  1:494941/46‑1 (MQ=255)
                             cTTTATCTGAAATTGCATTCAACTGACGGATTAATCGTCAATTTaa                                                             <  1:194866/46‑1 (MQ=255)
                             cTTTATCTGAAATTGCATTCAACTGACGGATTAATCGTCAATTTaa                                                             <  1:514590/46‑1 (MQ=255)
                             cTTTATCTGAAATTGCATTCAACTGACGGATTAATCGTCAATTTaa                                                             <  1:990149/46‑1 (MQ=255)
                             cTTTATCTGAAATTGCATTCAACTGACGGATTAATCGTCAATTTaa                                                             <  1:3252277/46‑1 (MQ=255)
                             cTTTATCTGAAATTGCATTCAACTGACGGATTAATCGTCAATTTaa                                                             <  1:1828604/46‑1 (MQ=255)
                             cTTTATCTGAAATTGCATTCAACTGACGGATTAATCGTCAATTTaa                                                             <  1:600728/46‑1 (MQ=255)
                             cTTTATCTGAAATTGCATTCAACTGACGGATTAATCGTCAATTTaa                                                             <  1:1324327/46‑1 (MQ=255)
                             cTTTATCTGAAATTGCATTCAACTGACGGATTAATCGTCAATTTaa                                                             <  1:904614/46‑1 (MQ=255)
                             cTTTATCTGAAATTGCATTCAACTGACGGATTAATCGTCAATTTaa                                                             <  1:2998629/46‑1 (MQ=255)
                             cTTTATCTGAAATTGCATTCAACTGACGGATTAATCGTCAATTTaa                                                             <  1:1437608/46‑1 (MQ=255)
                             cTTTATCTGAAATTGCATTCAACTGACGGATTAATCGTCAATTTaa                                                             <  1:903641/46‑1 (MQ=255)
                             cTTTATCTGAAATTGCATTCAACTGACGGATTAATCGTCAATTTAAGAGAAAGAGTTACACCGTcacca                                      >  1:1838310/1‑69 (MQ=255)
                                  tCTGAAATTGCATTCAACTGACGGATTAATCGTCAATTTAAGAGAAAGAGTTACACCGTCACCACTTc                                  <  1:820301/68‑1 (MQ=255)
                                                cAACTGTCGGATTAATCGTCAATTTAAGAGAAAGAGTTACACCGTCACCACTTCCGTGCACTGTATaaa                   >  1:8427/1‑69 (MQ=255)
                                                cAACTGACGGATTAATCGTCAATTTAAGAGAAAGAGTTACACCGTCACCACTTCCGTGCACTGTATaaa                   <  1:2639455/69‑1 (MQ=255)
                                                cAACTGACGGATTAATCGTCAATTTAAGAGAAAGAGTTACACCGTCACCACTTCCGTGCACTGTATaaa                   <  1:2037206/69‑1 (MQ=255)
                                                cAACTGACGGATTAATCGTCAATTTAAGAGAAAGAGTTACACCGTCACCACTTCCGTGCACTGTATaaa                   <  1:3261652/69‑1 (MQ=255)
                                                cAACTGACGGATTAATCGTCAATTTAAGAGAAAGAGTTACACCGTCACCACTTCCGTGCACTGTATaaa                   <  1:1882440/69‑1 (MQ=255)
                                                cAACTGACGGATTAATCGTCAATTTAAGAGAAAGAGTTACACCGTCACCACTTCCGTGCACTGTATaaa                   <  1:2299715/69‑1 (MQ=255)
                                                cAACTGACGGATTAATCGTCAATTTAAGAGAAAGAGTTACACCGTCACCACTTCCGTGCACTGTATaaa                   <  1:1285118/69‑1 (MQ=255)
                                                cAACTGACGGATTAATCGTCAATTTAAGAGAAAGAGTTACACCGTCACCACTTCCGTGCACTGTATaaa                   <  1:1288976/69‑1 (MQ=255)
                                                cAACTGACGGATTAATCGTCAATTTAAGAGAAAGAGTTACACCGTCACCACTTCCGTGCACTGTATaaa                   <  1:623819/69‑1 (MQ=255)
                                                cAACTGACGGATTAATCGTCAATTTAAGAGAAAGAGTTACACCGTCACCACTTCCGTGCACTGTATaaa                   >  1:3043044/1‑69 (MQ=255)
                                                cAACTGACGGATTAATCGTCAATTTAAGAGAAAGAGTTACACCGTCACCACTTCCGTGCACTGTATaaa                   <  1:1743710/69‑1 (MQ=255)
                                                cAACTGACGGATTAATCGTCAATTTAAGAGAAAGAGTTACACCGTCACCACTTCCGTGCACTGTATaaa                   <  1:1202058/69‑1 (MQ=255)
                                                cAACTGACGGATTAATCGTCAATTTAAGAGAAAGAGTTACACCGTCACCACTTCCGTGCACTGTATaaa                   <  1:771113/69‑1 (MQ=255)
                                                cAACTGACGGATTAATCGTCAATTTAAGAGAAAGAGTTACACCGTCACCACTTCCGTGCACTGTATaaa                   <  1:2801959/69‑1 (MQ=255)
                                                cAACTGACGGATTAATCGTCAATTTAAGAGAAAGAGTTACACCGTCACCACTTCCGTGCACTGTATaaa                   <  1:75951/69‑1 (MQ=255)
                                                cAACTGACGGATTAATCGTCAATTTAAGAGAAAGAGTTACACCGTCACCACTTCCGTGCACTGTATaaa                   <  1:2749666/69‑1 (MQ=255)
                                                cAACTGACGGATTAATCGCCAATTTAAGAGAAtgag                                                    >  1:812521/1‑36 (MQ=37)
                                                cAACTGACGGATTAATCGCCAATTTAAGAGAAagag                                                    >  1:789312/1‑36 (MQ=255)
                                                cAACTGACGGATTAATCGCCAATTTAAGAGAAagag                                                    >  1:85240/1‑36 (MQ=255)
                                                cAACTGACGGATTAATCGCCAATTTAAGAGAAagag                                                    >  1:86712/1‑36 (MQ=255)
                                                cAACTGACGGATTAATCGCCAATTTAAGAGAAagag                                                    >  1:297121/1‑36 (MQ=255)
                                                cAACTGACGGATTAATCGCCAATTTAAGAGAAagag                                                    >  1:3398796/1‑36 (MQ=255)
                                                cAACTGACGGATTAATCGCCAATTTAAGAGAAagag                                                    >  1:1234601/1‑36 (MQ=255)
                                                cAACTGACGGATTAATCGCCAATTTAAGAGAAagag                                                    >  1:1420155/1‑36 (MQ=255)
                                                cAACTGACGGATTAATCGCCAATTTAAGAGAAagag                                                    >  1:1567346/1‑36 (MQ=255)
                                                cAACTGACGGATTAATCGCCAATTTAAGAGAAagag                                                    >  1:1710950/1‑36 (MQ=255)
                                                cAACTGACGGATTAATCGCCAATTTAAGAGAAagag                                                    >  1:1917708/1‑36 (MQ=255)
                                                cAACTGACGGATTAATCGCCAATTTAAGAGAAagag                                                    >  1:1946814/1‑36 (MQ=255)
                                                cAACTGACGGATTAATCGCCAATTTAAGAGAAagag                                                    >  1:2416422/1‑36 (MQ=255)
                                                cAACTGACGGATTAATCGCCAATTTAAGAGAAagag                                                    >  1:2592493/1‑36 (MQ=255)
                                                cAACTGACGGATTAATCGCCAATTTAAGAGAAagag                                                    >  1:263177/1‑36 (MQ=255)
                                                cAACTGACGGATTAATCGCCAATTTAAGAGAAagag                                                    >  1:3156398/1‑36 (MQ=255)
                                                cAACTGACGGATTAATCGCCAATTTAAGAGAAagag                                                    >  1:3242152/1‑36 (MQ=255)
                                                cAACTGACGGATTAATCGCCAATTTAAGAGAAagag                                                    >  1:3389754/1‑36 (MQ=255)
                                                cAACTGACGGATTAATCGCCAATTTAAGAGAAagag                                                    >  1:408890/1‑36 (MQ=255)
                                                cAACTGACGGATTAATCGCCAATTTAAGAGAAagac                                                    >  1:1571731/1‑35 (MQ=38)
                                                cAACTGACGCATTAATCGCCAATTTAAGAGAAagag                                                    >  1:603078/1‑36 (MQ=37)
                                                    tGACGGATTAATCGTCAATTTAAGAGAAAGAGTTACACCGTCACCACTTCCGTGCACTGTATAAAAATc               <  1:2557641/69‑1 (MQ=255)
                                                    tGACGGATTAATCGTCAATTTAAGAGAAAGAGTTACACCGTCACCACTTCCGTGCACTGTATAAAAATc               <  1:2407799/69‑1 (MQ=255)
                                                        ggATTAATCGTCAATTTAAGAGAAAGAGTTACACCGTCACCACTTCCGTGCACTGTATAAAAATCCta            <  1:2031209/68‑1 (MQ=255)
                                                                 gTCAATTTAAGAGAAAGAGTTACACCGTCACCACTTCCGTGCACTGTATAAAAATCCTATACTGTACGt  <  1:3324922/69‑1 (MQ=255)
                                                                 gTCAATTTAAGAGAAAGAGTTACACCGTCACCACTTCCGTGCACTGTATAAAAATCCTATACTGTACGt  <  1:515730/69‑1 (MQ=255)
                                                                  |                                                                   
CAGGGGCAGTGATCTCGCTGCCCCTGGTTCTTTATCTGAAATTGCATTCAACTGACGGATTAATCGTCAATTTAAGAGAAAGAGTTACACCGTCACCACTTCCGTGCACTGTATAAAAATCCTATACTGTACGT  >  minE/1137744‑1137877

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: