| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,138,447 | G→A | 36.8% | L182L (TTG→TTA) | ydjM → | predicted inner membrane protein regulated by LexA |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,138,447 | 0 | G | A | 36.8% | 74.5 / 70.1 | 114 | L182L (TTG→TTA) | ydjM | predicted inner membrane protein regulated by LexA |
| Reads supporting (aligned to +/- strand): ref base G (51/21); new base A (25/17); total (76/38) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.25e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.87e-01 | |||||||||||
Only 100 of 114 total aligned reads displayed.
GATGCCCCATTCATTACCCGAGAACAGCGCTGTTCGTTGGTCATCGCAAATGATCAATACCTTGCAGATCCAGTTTCATCGGCTTATTAAGCATCAGGTTGAATACTAAAAAGGCAAAAATCACCTTT > minE/1138384‑1138511 | gATGCCCCATTCATTACCCGAGAAGAGCGCTGTTCGTTGGTCATCGCAAATGATCAATACCTTGCAGa > 1:274129/1‑68 (MQ=255)gATGCCCCATTCATTACCCGAGAACAGCGCTGTTCGTTGGTCATCGCAAATGATCAATACCTTGCa > 1:1045480/1‑66 (MQ=255)gATGCCCCATTCATTACCCGAGAACAGCGCTGTTCGTTGGTCATCGCAAATGATCAATACCTTGCAGa > 1:3128530/1‑68 (MQ=255)gATGCCCCATTCATTACCCGAGAACAGCGCTGTTCGTTGGTCATCGCAAATGATCAATACCTTGCAGa > 1:676369/1‑68 (MQ=255)gATGCCCCATTCATTACCCGAGAACAGCGCTGTTCGTTGGTCATCGCAAATGATCAATACCTTGCAGa > 1:3345055/1‑68 (MQ=255)gATGCCCCATTCATTACCCGAGAACAGCGCTGTTCGTTGGTCATCGCAAATGATCAATACCTTGCAGa > 1:1130312/1‑68 (MQ=255)gATGCCCCATTCATTACCCGAGAACAGCGCTGTTCGTTGGTCATCGCAAATGATCAATACCTTGCAGa > 1:3258397/1‑68 (MQ=255)gATGCCCCATTCATTACCCGAGAACAGCGCTGTTCGTTGGTCAACGCAAATGATCAATACCTTGCAg > 1:2320035/1‑67 (MQ=255) tGCCCCATTCATTACCCGAGAACAGCGCTGTTCGTTGGTCATCGCAAATGATCAATACCTTGCAGATcc < 1:2997942/69‑1 (MQ=255) attcattACCCGAAAACAGAGCTGTTCGTTGGTCATCGCAAATGAT‑AATACCTTACAGATCCAGTTTCa < 1:1266655/69‑1 (MQ=255) ttcattACCCGAGAACAGCGCTGTTCGTTGGTCATCGCAAATGATCAATACCTTGCAGATCCAGTTTCa > 1:2015636/1‑69 (MQ=255) ttcattACCCGAGAACAGCGCTGTTCGTTGGTCATCGCAAATGATCAATACCTTGCAGATCCAGTTTCa > 1:3342727/1‑69 (MQ=255) ttcattACCCGAGAACAGCGCTGTTCGTTGGTCATCGCAAATGATCAATACCTTGCAGATCCAGTTTCa > 1:3250096/1‑69 (MQ=255) ttcattACCCGAGAACAGCGCTGTTCGTTGGTCATCGCAAATGATCAATACCTTGCAGATCCAGTTTCa > 1:3009701/1‑69 (MQ=255) ttcattACCCGAGAACAGCGCTGTTCGTTGGTCATCGCAAATGATCAATACCTTGCAGATCCAGTTTCa > 1:1832741/1‑69 (MQ=255) ttcattACCCGAGAACAGCGCTGTTCGTTGGTCATCGCAAATGATCAATACCTTGCAGATCCAGTTTCa > 1:3075470/1‑69 (MQ=255) ttcattACCCGAGAACAGCGCTGTTCGTTGGTCATCGCAAATGATCAATACCTTGCAGATCCAGTTTCa > 1:708490/1‑69 (MQ=255) ttcattACCCGAGAACAGCGCTGTTCGTTGGTCATCGCAAATGATCAATACCTTGCAGATCCAGTTTCa > 1:1616830/1‑69 (MQ=255) ttcattACCCGAGAACAGCGCTGTTCGTTGGTCATCGCAAATGATCAATACCTTGCAGATCCAGTTTCa > 1:1297797/1‑69 (MQ=255) ttcattACCCGAGAACAGCGCTGTTCGTTGGTCATCGCAAATGATCAATACCTTGCAGATCCAGTTTCa > 1:1294925/1‑69 (MQ=255) ttcattACCCGAGAACAGCGCTGTTCGTTGGTCATCGCAAATGATCAATACCTTGCAGATCCAGTTTCa > 1:1517227/1‑69 (MQ=255) ttcattACCCGAGAACAGCGCTGTTCGTTGGTCATCGCAAATGATCAATACCTTGCAGATCCAGTTTCa > 1:1293352/1‑69 (MQ=255) ttcattACCCGAAAACAGCGCTGTTCGTTGGTCATCGCAAATGATCAATACCTTACAGATCCAGTTTCa < 1:1109259/69‑1 (MQ=255) ttcattACCCGAAAACAGCGCTGTTCGTTGGTCATCGCAAATGATCAATACCTTACAGATCCAGTTTCa < 1:2155671/69‑1 (MQ=255) ttcattACCCGAAAACAGCGCTGTTCGTTGGTCATCGCAAATGATCAATACCTTACAGATCCAGTTTCa < 1:3038502/69‑1 (MQ=255) ttcattACCCGAAAACAGCGCTGTTCGTTGGTCATCGCAAATGATCAATACCTTACAGATCCAGTTTCa < 1:2407157/69‑1 (MQ=255) ttcattACCCGAAAACAGCGCTGTTCGTTGGTCATCGCAAATGATCAATACCTTACAGATCCAGTTTCa < 1:2507251/69‑1 (MQ=255) ttcattACCCGAAAACAGCGCTGTTCGTTGGTCATCGCAAATGATCAATACCTTACAGATCCAGTTTCa < 1:2641839/69‑1 (MQ=255) ttcattACCCGAAAACAGCGCTGTTCGTTGGTCATCGCAAATGATCAATACCTTACAGATCCAGTTTCa < 1:901420/69‑1 (MQ=255) ttcattACCCGAAAACAGCGCTGTTCGTTGGTCATCGCAAATGATCAATACCTTACAGATCCAGTTTCa < 1:3193761/69‑1 (MQ=255) ttcattACCCGAAAACAGCGCTGTTCGTTGGTCATCGCAAATGATCAATACCTTACAGATCCAGTTTCa < 1:732250/69‑1 (MQ=255) ttcattACCCGAAAACAGCGCTGTTCGTTGGTCATCGCAAATGATCAATACCTTACAGATCCAGTTTCa < 1:298581/69‑1 (MQ=255) ttcattACCCGAAAACAGCGCTGTTCGTTGGTCATCGCAAATGATCAATACCTTACAGATCCAGTTTCa < 1:3159974/69‑1 (MQ=255) tcattACCCGAAAACAGCGCTGTTCGTTGGTCATCGCAAATGATCAATACCTTACAGATCCAGTTTCa < 1:482360/68‑1 (MQ=255) tattACCCGAGAACAGCGCTGTTCGTTGGTCATCGCAAATGATCAATACCTTGCAGATCCAGTTTCAt > 1:1025661/2‑68 (MQ=255) tattACCCGAGAACAGCGCTGTTCGTTGGTCATCGCAAATGATCAATACCTTGCAGATCCAGTTTCAt > 1:2838014/2‑68 (MQ=255) tattACCCGAGAACAGCGCTGTTCGTTGGTCATCGCAAATGATCAATACCTTGCAGATCCAGTTTCATc > 1:3420500/2‑69 (MQ=255) tattACCCGAGAACAGCGCTGTTCGTTGGTCATCGCAAATGATCAATACCTTGCAGATCCAGTTTCATc > 1:1396948/2‑69 (MQ=255) cattACCCGAGAACAGCGCTGTTCGTTGGTCATCGCAAATGATCAATACCTTGCAGATCCAGTTTCAt > 1:708999/1‑68 (MQ=255) aCCCGAGAACAGCGCTGTTCGTTGGTCATCGCAAATGATCAATACCTTGCAGATCCAGttt > 1:1925147/1‑61 (MQ=255) aCCCGAGAACAGCGCTGTTCGTTGGTCATCGCAAATGATCAATACCTTGCAGATCCAGTTTc > 1:3396498/1‑62 (MQ=255) aCCCGAGAACAGCGCTGTTCGTTGGTCATCGCAAATGATCAATACCTTGCAGATCCAGTTTc > 1:867911/1‑62 (MQ=255) aCCCGAGAACAGCGCTGTTCGTTGGTCATCGCAAATGATCAATACCTTGCAGATCCAGTTTc > 1:668494/1‑62 (MQ=255) aCCCGAAAACAGCGCTGTTCGTTGGTCATCGCAAATGATCAATACCTTACAGATCCAGTTTCa < 1:1406311/63‑1 (MQ=255) aCAGCGCTGTTCGTTGGTCATCGCAAATGATCAATACCTTGCAGATCCAGTTTCATCGGCTTATTAAGc < 1:1591552/69‑1 (MQ=255) gcgcTGTTCGTTGGTCATCGCAAATGATCAATACCTTACAGATCCAGTTTCa < 1:118677/52‑1 (MQ=255) cGTTGGTCATCGCAAATGATCAATACCTTGCAGATCCAGTTTCATCGGCTTATTAAGCATCAGGTTg < 1:3151039/67‑1 (MQ=255) cGTTGGTCATCGCAAATGATCAATACCTTGCAGATCCAGTTTCATCGGCTTATTAAGCATCAGGTTg < 1:1516760/67‑1 (MQ=255) cGTTGGTCATCGCAAATGATCAATACCTTGCAGATCCAGTTTCATCGGCTTATTAAGCATCAGGTTg < 1:1694420/67‑1 (MQ=255) cGTTGGTCATCGCAAATGATCAATACCTTGCAGATCCAGTTTCATCGGCTTATTAAGCATCAGGTTg < 1:285598/67‑1 (MQ=255) gTTGGTCATCGCAAATGATCAATACCTTGCAGATCCAGTTTCATCGGCTTATTAAGCATCAGGTTg < 1:1068350/66‑1 (MQ=255) gCAAATGATCAATACCTTGCAGATCCAGTTTCATCGGCTTATTAAGCATCAGGTTGaa < 1:245124/58‑1 (MQ=255) gCAAATGATCAATACCTTGCAGATCCAGTTTCATCGGCTTATTAAGCATCAGGTTGaa < 1:3300866/58‑1 (MQ=255) gCAAATGATCAATACCTTGCAGATCCAGTTTCATCGGCTTATTAAGCATCAGGTTGaa < 1:1554987/58‑1 (MQ=255) gCAAATGATCAATACCTTGCAGATCCAGTTTCATCGGCTTATTAAGCATCAGGTTGaa < 1:2348446/58‑1 (MQ=255) gCAAATGATCAATACCTTGCAGATCCAGTTTCATCGGCTTATTAAGCATCAGGTTGaa < 1:358780/58‑1 (MQ=255) gCAAATGATCAATACCTTGCAGATCCAGTTTCATCGGCTTATTAAGCATCAGGTTGaa < 1:3211805/58‑1 (MQ=255) gCAAATGATCAATACCTTGCAGATCCAGTTTCATCGGCTTATTAAGCATCAGGTTGaa < 1:354794/58‑1 (MQ=255) gCAAATGATCAATACCTTGCAGATCCAGTTTCATCGGCTTATTAAGCATCAGGTTGAATACTAAAAAg > 1:2381308/1‑68 (MQ=255) gCAAATGATCAATACCTTGCAGATCCAGTTTCATCGGCTTATTAAGCATCAGGTTGAATACTAAAAAg > 1:3170706/1‑68 (MQ=255) gCAAATGATCAATACCTTGCAGATCCAGTTTCATCGGCTTATTAAGCATCAGGTTGAATACTAAAAAg > 1:2717632/1‑68 (MQ=255) gCAAATGATCAATACCTTGCAGATCCAGTTTCATCGGCTTATTAAGCATCAGGTTGAATACTAAAAAg > 1:1215536/1‑68 (MQ=255) gCAAATGATCAATACCTTGCAGATCCAGTTTCATCGGCTTATTAAGCATCAGGTTGAATACTAAAAAg > 1:2465869/1‑68 (MQ=255) gCAAATGATCAATACCTTGCAGATCCAGTTTCATCGGCTTATTAAGCATCAGGTTGAATACTAAAAAg > 1:1940520/1‑68 (MQ=255) gCAAATGATCAATACCTTGCAGATCCAGTTTCATCGGCTTATTAAGCATCAGGTTGAATACTAAAAAg > 1:818958/1‑68 (MQ=255) aaaTGATCAATACCTTGCAGATCCAGTTTCATCGGCTTATTAAGCATCAGGTTGAATACTAAAAAGGCa < 1:1026199/69‑1 (MQ=255) aaaTGATCAATACCTTGCAGATCCAGTTTCATCGGCTTATTAAGCATCAGGTTGAATACTAAAAAGGCa < 1:1698441/69‑1 (MQ=255) aaaTGATCAATACCTTGCAGATCCAGTTTCATCGGCTTATTAAGCATCAGGTTGAATACTAAAAAGGCa < 1:3029392/69‑1 (MQ=255) tGATCAATACCTTGCAGATCCAGTTTCATCGGCTTATTAAGCATCAGGTTGAATACTAAAAAGGCaaaa > 1:2922793/1‑69 (MQ=255) tGATCAATACCTTGCAGATCCAGTTTCATCGGCTTATTAAGCATCAGGTTGAATACTAAAAAGGCaaaa > 1:564995/1‑69 (MQ=255) tGATCAATACCTTGCAGATCCAGTTTCATCGGCTTATTAAGCATCAGGTTGAATACTAAAAAGGCaaaa > 1:1610509/1‑69 (MQ=255) tGATCAATACCTTGCAGATCCAGTTTCATCGGCTTATTAAGCATCAGGTTGAATACTAAAAAGGCaaaa > 1:2284760/1‑69 (MQ=255) tGATCAATACCTTGCAGATCCAGTTTCATCGGCTTATTAAGCATCAGGTTGAATACTAAAAAGGCaaaa > 1:825370/1‑69 (MQ=255) tGATCAATACCTTGCAGATCCAGTTTCATCGGCTTATTAAGCATCAGGTTGAATACTAAAAAGGCaaaa > 1:2868969/1‑69 (MQ=255) tGATCAATACCTTGCAGATCCAGTTTCATCGGCTTATTAAGCATCAGGTTGAATACTAAAAAGGCaaa > 1:260926/1‑68 (MQ=255) gATCAATAGCTTACAGATCCAGTTTCATCGGCTTATTAAGc > 1:3200707/1‑41 (MQ=38) gATCAATACCTTACAGATCCAGTTTCATCGGCTTATTAAg > 1:2171314/1‑40 (MQ=255) gATCAATACCTTACAGATCCAGTTTCATCGGCTTATTAAGc > 1:1482266/1‑41 (MQ=255) gATCAATACCTTACAGATCCAGTTTCATCGGCTTATTAAGc > 1:824990/1‑41 (MQ=255) gATCAATACCTTACAGATCCAGTTTCATCGGCTTATTAAGc > 1:1588583/1‑41 (MQ=255) gATCAATACCTTACAGATCCAGTTTCATCGGCTTATTAAGc > 1:1592427/1‑41 (MQ=255) gATCAATACCTTACAGATCCAGTTTCATCGGCTTATTAAGc > 1:1130592/1‑41 (MQ=255) gATCAATACCTTACAGATCCAGTTTCATCGGCTTATTAAGc > 1:1755850/1‑41 (MQ=255) gATCAATACCTTACAGATCCAGTTTCATCGGCTTATTAAGc > 1:1862938/1‑41 (MQ=255) gATCAATACCTTACAGATCCAGTTTCATCGGCTTATTAAGc > 1:646516/1‑41 (MQ=255) gATCAATACCTTACAGATCCAGTTTCATCGGCTTATTAAGc > 1:639233/1‑41 (MQ=255) gATCAATACCTTACAGATCCAGTTTCATCGGCTTATTAAGc > 1:937747/1‑41 (MQ=255) gATCAATACCTTACAGATCCAGTTTCATCGGCTTATTAAGc > 1:559340/1‑41 (MQ=255) gATCAATACCTTACAGATCCAGTTTCATCGGCTTATTAAGc > 1:1933521/1‑41 (MQ=255) gATCAATACCTTACAGATCCAGTTTCATCGGCTTATTAAGc > 1:376152/1‑41 (MQ=255) gATCAATACCTTACAGATCCAGTTTCATCGGCTTATTAAGc > 1:1945515/1‑41 (MQ=255) gATCAATACCTTACAGATCCAGTTTCATCGGCTTATTAAGc > 1:219484/1‑41 (MQ=255) gATCAATACCTTACAGATCCAGTTTCATCGGCTTATTAAGc > 1:2270404/1‑41 (MQ=255) gATCAATACCTTACAGATCCAGTTTCATCGGCTTATTAAGc > 1:3289121/1‑41 (MQ=255) gATCAATACCTTACAGATCCAGTTTCATCGGCTTATTAAGc > 1:3285270/1‑41 (MQ=255) gATCAATACCTTACAGATCCAGTTTCATCGGCTTATTAAGc > 1:2478951/1‑41 (MQ=255) gATCAATACCTTACAGATCCAGTTTCATCGGCTTATTAAGc > 1:2706899/1‑41 (MQ=255) gATCAATACCTTAAAGATCCAGTTTCATCGGCTTATTAAGc > 1:2339070/1‑41 (MQ=38) ccTTGCAGATCCAGTTTCATCGGCTTATTAAGCATCAGGTTGAATACTAAAAAGGCAAAAATCACCttt > 1:2474973/1‑69 (MQ=255) ccTTGCAGATCCAGTTTCATCGGCTTATTAAGCATCAGGTTGAATACTAAAAAGGCAAAAATCACCttt > 1:2796960/1‑69 (MQ=255) | GATGCCCCATTCATTACCCGAGAACAGCGCTGTTCGTTGGTCATCGCAAATGATCAATACCTTGCAGATCCAGTTTCATCGGCTTATTAAGCATCAGGTTGAATACTAAAAAGGCAAAAATCACCTTT > minE/1138384‑1138511 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |