BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	minE	1,138,871	T→C	36.2%	L83L (TTA→CTA)	ydjN →	predicted transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	1,138,871	0	T	C	36.2%	60.4 / 63.6	102	L83L (TTA→CTA)	ydjN	predicted transporter
Reads supporting (aligned to +/- strand): ref base T (20/45); new base C (11/26); total (31/71)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.47e-01

Only 100 of 109 total aligned reads displayed.

TTCTGTACAGTGGTTTAACATCGTTGGTAACGGCTATGTTCAACTGCTGCAAATGATCGTTATGCCGTTAGTCTTCGCCTCTATTCTGAGCGCGGTTGCCCGTCTGCATAACGCATCTCAGTTAG  >  minE/1138804‑1138928

ttCTGTACAGTGGTTTAACATCGTTGGTAACGGCTATGTTCAACTGCTGCAAATGATCGTTATGCCGtt                                                          <  1:2803504/69‑1 (MQ=255)

ttCTGTACAGTGGTTTAACATCGTTGGTAACGGCTATGTTCAACTGCTGCAAATGATCGTTATGCCGtt                                                          <  1:744157/69‑1 (MQ=255)

ttCTGTACAGTGGTTTAACATCGTTGGTAACGGCTATGTTCAACTGCTGCAAATGATCGTTATGCCGtt                                                          <  1:3158600/69‑1 (MQ=255)

ttCTGTACAGTGGTTTAACATCGTTGGTAACGGCTATGTTCAACTGCTGCAAATGATCGTTATGCCGtt                                                          <  1:2086299/69‑1 (MQ=255)

ttCTGTACAGTGGTTTAACATCGTTGGTAACGGCTATGTTCAACTGCTGCAAATGATCGTTATGCCGtt                                                          <  1:2765010/69‑1 (MQ=255)

     tACAGTGGTTTAACATTGTTGGTAACGGCTATGTTCAACTGCTGCAAATGATCGTTATGCCGCTAGTc                                                      >  1:345599/1‑68 (MQ=255)

     tACAGTGGTTTAACATTGTTGGTAACGGCTATGTTCAACTGCTGCAAATGATCGTTATGCCGCTAGTc                                                      >  1:1519625/1‑68 (MQ=255)

     tACAGTGGTTTAACATTGTTGGTAACGGCTATGTTCAACTGCTGCAAATGATCGTTATGCCGCTAGTCt                                                     >  1:2770344/1‑69 (MQ=255)

     tACAGTGGTTTAACATTGTTGGTAACGGCTATGTTCAACTGCTGCAAATGATCGTTATGCCGCTAGTCt                                                     >  1:238680/1‑69 (MQ=255)

     tACAGTGGTTTAACATTGTTGGTAACGGCTATGTTCAACTGCTGCAAATGATCGTTATGCCGCTAGTCt                                                     >  1:303859/1‑69 (MQ=255)

     tACAGTGGTTTAACATTGTTGGTAACGGCTATGTTCAACTGCTGCAAATGATCGTTATGCCGCTAGTCt                                                     >  1:1743042/1‑69 (MQ=255)

     tACAGTGGTTTAACATTGTTGGTAACGGCTATGTTCAACTGCTGCAAATGATCGTTATGCCGCTAGTCt                                                     >  1:1654307/1‑69 (MQ=255)

     tACAGTGGTTTAACATTGTTGGTAACGGCTATGTTCAACTGCTGCAAATGATCGTTATGCCGCTAGTCt                                                     >  1:3264521/1‑69 (MQ=255)

     tACAGTGGTTTAACATTGTTGGTAACGGCTATGTTCAACTGCTGCAAATGATCGTTATGCCGCTAGTCt                                                     >  1:1563498/1‑69 (MQ=255)

     tACAGTGGTTTAACATTGTTGGTAACGGCTATGTTCAACTGCTGCAAATGATCGTTATGCCGCTAGTCt                                                     >  1:3275686/1‑69 (MQ=255)

     tACAGTGGTTTAACATTGTTGGTAACGGCTATGTTCAACTGCTGCAAATGATCGTTATGCCGCTAGTCt                                                     >  1:691849/1‑69 (MQ=255)

          tGGTTTAACATCGTTGGTAACGGCTATGTTCAACTGCTGCAAATGATCGTTATGCCGTTAGTCTTCGcc                                                <  1:1481017/69‑1 (MQ=255)

          tGGTTTAACATCGTTGGTAACGGCTATGTTCAACTGCTGCAAATGATCGTTATGCCGTTAGTCTTCGcc                                                <  1:2983915/69‑1 (MQ=255)

          tGGTTTAACATCGTTGGTAACGGCTATGTTCAACTGCTGCAAATGATCGTTATGCCGTTAGTCTTCGcc                                                <  1:214613/69‑1 (MQ=255)

          tGGTTTAACATCGTTGGTAACGGCTATGTTCAACTGCTGCAAATGATCGTTATGCCGTTAGTCTTCGcc                                                <  1:809406/69‑1 (MQ=255)

          tGGTTTAACATCGTTGGTAACGGCTATGTTCAACTGCTGCAAATGATCGTTATGCCGTTAGTCTTCGcc                                                <  1:2456947/69‑1 (MQ=255)

          tGGTTTAACATCGTTGGTAACGGCTATGTTCAACTGCTGCAAATGATCGTTATGCCGTTAGTCTTCGcc                                                <  1:1312222/69‑1 (MQ=255)

          tGGTTTAACATCGTTGGTAACGGCTATGTTCAACTGCTGCAAATGATCGTTATGCCGTTAGTCTTCGcc                                                <  1:45384/69‑1 (MQ=255)

            gTTTAACATCGTTGGTAACGGCTATGTTCAACTGCTGCAAATGATCGTTATGCCGTTAGTc                                                      <  1:1506743/61‑1 (MQ=255)

            gTTTAACATCGTTGGTAACGGCTATGTTCAACTGCTGCAAATGATCGTTATGCCGTTAGTCTTCGCctc                                              <  1:2850109/69‑1 (MQ=255)

            gTTTAACATCGTTGGTAACGGCTATGTTCAACTGCTGCAAATGATCGTTATGCCGTTAGTCTTCGCctc                                              <  1:318363/69‑1 (MQ=255)

            gTTTAACATCGTTGGTAACGGCTATGTTCAACTGCTGCAAATGATCGTTATGCCGTTAGTCTTCGCctc                                              <  1:2750978/69‑1 (MQ=255)

            gTTTAACATCGTTGGTAACGGCTATGTTCAACTGCTGCAAATGATCGTTATGCCGTTAGTCTTCGCctc                                              <  1:711681/69‑1 (MQ=255)

            gTTTAACATCGTTGGTAACGGCTATGTTCAACTGCTGCAAATGATCGTTATGCCGTTAGTCTTCGCctc                                              <  1:314422/69‑1 (MQ=255)

            gTTTAACATCGTTGGTAACGGCTATGTTCAACTGCTGCAAATGATCGTTATGCCGTTAGTCTTCGCctc                                              <  1:2994618/69‑1 (MQ=255)

            gTTTAACATCGTTGGTAACGGCTATGTTCAACTGCTGCAAATGATCGTTATGCCGTTAGTCTTCGCctc                                              <  1:1420689/69‑1 (MQ=255)

            gTTTAACATCGTTGGTAACGGCTATGTTCAACTGCTGCAAATGATCGTTATGCCGTTAGTCTTCGCctc                                              <  1:290632/69‑1 (MQ=255)

               tAACATCGTTGGTAACGGCTATGTTCAACTGCTGCAAATGATCGTTATGCCGTTAGTCTTCGCCTCTAt                                           >  1:2777086/1‑69 (MQ=255)

               tAACATCGTTGGTAACGGCTATGTTCAACTGCTGCAAATGATCGTTATGCCGTTAGTCTTCGCCTCTAt                                           >  1:2125511/1‑69 (MQ=255)

               tAACATCGTTGGTAACGGCTATGTTCAACTGCTGCAAATGATCGTTATGCCGTTAGTCTTCGCCTCTAt                                           >  1:3373224/1‑69 (MQ=255)

               tAACATCGTTGGTAACGGCTATGTTCAACTGCTGCAAATGATCGTTATGCCGTTAGTCTTCGCCTCTAt                                           >  1:1311255/1‑69 (MQ=255)

               tAACATCGTTGGTAACGGCTATGTTCAACTGCTGCAAATGATCGTTATGCCGTTAGTCTTCGCCTCTAt                                           >  1:1304063/1‑69 (MQ=255)

               tAACATCGTTGGTAACGGCTATGTTCAACTGCTGCAAATGATCGTTATGCCGTTAGTCTTCGCCTCTAt                                           >  1:2461889/1‑69 (MQ=255)

               tAACATCGTTGGTAACGGCTATGTTCAACTGCTGCAAATGATCGTTATGCCGTTAGTCTTCGCCTCTAt                                           >  1:823163/1‑69 (MQ=255)

               tAACATCGTTGGTAACGGCTATGTTCAACTGCTGCAAATGATCGTTATGCCGTTAGTCTTCGCCTCTAt                                           >  1:2671761/1‑69 (MQ=255)

               tAACATCGTTGGTAACGGCTATGTACAACTGCTGCAAATGATCGTTATGCCGTTAGTCTTCGCCTCTAt                                           >  1:904308/1‑69 (MQ=255)

                 aCATCGTTGGTAACGGCTATGTTCAACTGCTGCAAATGATCGTTATGCCGtt                                                          <  1:1876424/52‑1 (MQ=255)

                    tCGTTGGTAACGGCTATGTTCAACTGCTGCAAATGATCGTTATGCCGTTAGTCTTCGcc                                                <  1:216474/59‑1 (MQ=255)

                      gTTGGTAACGGCTATGTTCAACTGCTGCAAATGATCGTTATGCCGTTAGTCTTCGCCTCTATTCTGAgc                                    >  1:446306/1‑69 (MQ=255)

                         ggTAACGGCTATGTTCAACTGCTGCAAATGATCGTTATGCCGTTAGTCTTCGCCTCTATTCTGAGcgcg                                 <  1:679785/69‑1 (MQ=255)

                                        cAACTGCTGCAAATGATCGTTATGCCGTTAGTCTTCGCCTCTATTCTAAGCGCGGTTGCCCGTCTGCAt                  <  1:2901301/69‑1 (MQ=255)

                                        cAACTGCTGCAAATGATCGTTATGCCGTTAGTCTTCGCCTCTATTCTAAGCGCGGTTGCCCGTCTGCAt                  <  1:1162500/69‑1 (MQ=255)

                                             gctgcATATGATCGTTATGCCGTTAGTCTTCGCCTCTAt                                           <  1:2872862/39‑1 (MQ=255)

                                             gctgcAAATGATCGTTATGCCGTTAGTCTTCGCCTCTAt                                           <  1:547759/39‑1 (MQ=255)

                                             gctgcAAATGATCGTTATGCCGTTAGTCTTCGCCTCTAt                                           <  1:2723256/39‑1 (MQ=255)

                                             gctgcAAATGATCGTTATGCCGTTAGTCTTCGCCTCTAt                                           <  1:2504168/39‑1 (MQ=255)

                                             gctgcAAATGATCGTTATGCCGTTAGTCTTCGCCTCTAt                                           <  1:2664301/39‑1 (MQ=255)

                                             gctgcAAATGATCGTTATGCCGTTAGTCTTCGCCTCTAt                                           <  1:1577122/39‑1 (MQ=255)

                                             gctgcAAATGATCGTTATGCCGTTAGTCTTCGCCTCTAt                                           <  1:1927631/39‑1 (MQ=255)

                                             gctgcAAATGATCGTTATGCCGTTAGTCTTCGCCTCTAt                                           <  1:1560837/39‑1 (MQ=255)

                                             gctgcAAATGATCGTTATGCCGTTAGTCTTCGCCTCTAt                                           <  1:2500640/39‑1 (MQ=255)

                                             gctgcAAATGATCGTTATGCCGTTAGTCTTCGCCTCTAt                                           <  1:1399700/39‑1 (MQ=255)

                                             gctgcAAATGATCGTTATGCCGTTAGTCTTCGCCTCTATTCTGAGCGCGGTTGCCCGTCTGCATAACg              >  1:2506279/1‑68 (MQ=255)

                                              ctgcAAATGATCGTTATGCCGTTAGTCTTCGCCTCTAt                                           <  1:2744382/38‑1 (MQ=255)

                                              ctgcAAATGATCGTTATGCCGTTAGTCTTCGCCTCTAt                                           <  1:1856525/38‑1 (MQ=255)

                                                   aaTGATCGTTATGCCGTTAGTCTTCGCCTCTATTCTGAGCGCGGTTGCCCGTCTGCATAACGCAtctc        <  1:2731030/68‑1 (MQ=255)

                                                   aaTGATCGTTATGCCGTTAGTCTTCGCCTCTATTCTGAGCGCGGTTGCCCGTCTGCATAACGCAtctc        <  1:2369912/68‑1 (MQ=255)

                                                   aaTGATCGTTATGCCGTTAGTCTTCGCCTCTATTCTGAGCGCGGTTGCCCGTCTGCATAACGCAtctc        <  1:1284359/68‑1 (MQ=255)

                                                      gATCGTTTTGCCGCTAGTCTTCGCCTCTATTCTGAGCGCGGTTGCCCGTCTGCATAACGCATCTCAGtt    <  1:3414594/69‑1 (MQ=255)

                                                      gATCGTTATGCCGTTAGTCTTCGCCTCTATTCTGAGCGCGGTTGCCCGTCTGCATAACGCATCTCAGtt    <  1:1282681/69‑1 (MQ=255)

                                                      gATCGTTATGCCGTTAGTCTTCGCCTCTATTCTGAGCGCGGTTGCCCGTCTGCATAACGCATCTCAGtt    <  1:1786697/69‑1 (MQ=255)

                                                      gATCGTTATGCCGTTAGTCTTCGCCTCTATTCTGAGCGCGGTTGCCCGTCTGCATAACGCATCTCAGtt    <  1:1782781/69‑1 (MQ=255)

                                                      gATCGTTATGCCGTTAGTCTTCGCCTCTATTCTGAGCGCGGTTGCCCGTCTGCATAACGCATCTCAGtt    <  1:2958167/69‑1 (MQ=255)

                                                      gATCGTTATGCCGTTAGTCTTCGCCTCTATTCTGAGCGCGGTTGCCCGTCTGCATAACGCATCTCAGtt    <  1:201501/69‑1 (MQ=255)

                                                      gATCGTTATGCCGTTAGTCTTCGCCTCTATTCTGAGCGCGGTTGCCCGTCTGCATAACGCATCTCAGtt    <  1:3289046/69‑1 (MQ=255)

                                                      gATCGTTATGCCGCTAGTCTTCGCCTCTATTCTGAGCGCGGTTGCCCGTCTGCATAACGCATCTCAGtt    <  1:3278762/69‑1 (MQ=255)

                                                      gATCGTTATGCCGCTAGTCTTCGCCTCTATTCTGAGCGCGGTTGCCCGTCTGCATAACGCATCTCAGtt    <  1:1540576/69‑1 (MQ=255)

                                                      gATCGTTATGCCGCTAGTCTTCGCCTCTATTCTGAGCGCGGTTGCCCGTCTGCATAACGCATCTCAGtt    <  1:1529621/69‑1 (MQ=255)

                                                      gATCGTTATGCCGCTAGTCTTCGCCTCTATTCTGAGCGCGGTTGCCCGTCTGCATAACGCATCTCAGtt    <  1:105743/69‑1 (MQ=255)

                                                      gATCGTTATGCCGCTAGTCTTCGCCTCTATTCTGAGCGCGGTTGCCCGTCTGCATAACGCATCTCAGtt    <  1:1070443/69‑1 (MQ=255)

                                                      gATCGTTATGCCGCTAGTCTTCGCCTCTATTCTGAGCGCGGTTGCCCGTCTGCATAACGCATCTCAGtt    <  1:1372711/69‑1 (MQ=255)

                                                      gATCGTTATGCCGCTAGTCTTCGCCTCTATTCTGAGCGCGGTTGCCCGTCTGCATAACGCATCTCAGtt    <  1:585699/69‑1 (MQ=255)

                                                      gATCGTTATGCCGCTAGTCTTCGCCTCTATTCTGAGCGCGGTTGCCCGTCTGCATAACGCATCTCAGtt    <  1:1265766/69‑1 (MQ=255)

                                                      gATCGTTATGCCGCTAGTCTTCGCCTCTATTCTGAGCGCGGTTGCCCGTCTGCATAACGCATCTCAGtt    <  1:2528296/69‑1 (MQ=255)

                                                      gATCGTTATGCCGCTAGTCTTCGCCTCTATTCTGAGCGCGGTTGCCCGTCTGCATAACGCATCTCAGtt    <  1:261975/69‑1 (MQ=255)

                                                      gATCGTTATGCCGCTAGTCTTCGCCTCTATTCTGAGCGCGGTTGCCCGTCTGCATAACGCATCTCAGtt    <  1:1968693/69‑1 (MQ=255)

                                                      gATCGTTATGCCGCTAGTCTTCGCCTCTATTCTGAGCGCGGTTGCCCGTCTGCATAACGCATCTCAGtt    <  1:2050587/69‑1 (MQ=255)

                                                      gATCGTTATGCCGCTAGTCTTCGCCTCTATTCTGAGCGCGGTTGCCCGTCTGCATAACGCATCTCAGtt    <  1:2125410/69‑1 (MQ=255)

                                                      gATCGTTATGCCGCTAGTCTTCGCCTCTATTCTGAGCGCGGTTGCCCGTCTGCATAACGCATCTCAGtt    <  1:2623894/69‑1 (MQ=255)

                                                      gATCGTTATGCCGCTAGTCTTCGCCTCTATTCTGAGCGCGGTTGCCCGTCTGCATAACGCATCTCAGtt    <  1:1599346/69‑1 (MQ=255)

                                                      gATCGTTATGCCGCTAGTCTTCGCCTCTATTCTGAGCGCGGTTGCCCGTCTGCATAACGCATCTCAGtt    <  1:241745/69‑1 (MQ=255)

                                                      gATCGTTATGCCGCTAGTCTTCGCCTCTATTCTGAGCGCGGTTGCCCGTCTGCATAACGCATCTCAGtt    <  1:241871/69‑1 (MQ=255)

                                                      gATCGTTATGCCGCTAGTCTTCGCCTCTATTCTGAGCGCGGTTGCCCGTCTGCATAACGCATCTCAGtt    <  1:274494/69‑1 (MQ=255)

                                                      gATCGTTATGCCGCTAGTCTTCGCCTCTATTCTGAGCGCGGTTGCCCGTCTGCATAACGCATCTCAGtt    <  1:1011380/69‑1 (MQ=255)

                                                      gATCGTTATGCCGCTAGTCTTCGCCTCTATTCTGAGAGCGGTTGCCCGTCTGCATAACGCATCTCAGtt    <  1:3197884/69‑1 (MQ=255)

                                                       aTCGTTATGCCGCTAGTCTTCGCCTCTATTCTGAGCGCGGTTGCCCGTCTGCATAACGCATCTCAGTTa   <  1:226349/69‑1 (MQ=255)

                                                       aTCGTTATGCCGCTAGTCTTCGCCTCTATTCTGAGCGCGGTTGCCCGTCTGCATAACGCATCTCAGTTa   <  1:2236626/69‑1 (MQ=255)

                                                       aTCGTTATGCCGCTAGTCTTCGCCTCTATTCTGAGCGCGGTTGCCCGTCTGCATAACGCATCTCAGTTa   <  1:1639094/69‑1 (MQ=255)

                                                         cGTTATGCCGTTAGTCTTCGCCTCTATTCTGAGCGCGGTTGCCCGTCTGCATAACGCATCTCAGTTAg  >  1:890052/1‑68 (MQ=255)

                                                         cGTTATGCCGTTAGTCTTCGCCTCTATTCTGAGCGCGGTTGCCCGTCTGCATAACGCATCTCAGTTAg  >  1:2571770/1‑68 (MQ=255)

                                                         cGTTATGCCGTTAGTCTTCGCCTCTATTCTGAGCGCGGTTGCCCGTCTGCATAACGCATCTCAGTTAg  >  1:1083917/1‑68 (MQ=255)

                                                         cGTTATGCCGTTAGTCTTCGCCTCTATTCTGAGCGCGGTTGCCCGTCTGCATAACGCATCTCAGTTAg  >  1:1423129/1‑68 (MQ=255)

                                                         cGTTATGCCGTTAGTCTTCGCCTCTATTCTGAGCGCGGTTGCCCGTCTGCATAACGCATCTCAGTTAg  >  1:392245/1‑68 (MQ=255)

                                                         cGTTATGCCGTTAGTCTTCGCCTCTATTCTGAGCGCGGTTGCCCGTCTGCATAACGCATCTCAGTTAg  >  1:2998302/1‑68 (MQ=255)

                                                             aTGCCGCTAGTCTTCGCCTCTATTCTGAGCGCGGTTGCCCGTCTGCATAACGCATCTCAGtt    <  1:1056902/62‑1 (MQ=255)

TTCTGTACAGTGGTTTAACATCGTTGGTAACGGCTATGTTCAACTGCTGCAAATGATCGTTATGCCGTTAGTCTTCGCCTCTATTCTGAGCGCGGTTGCCCGTCTGCATAACGCATCTCAGTTAG  >  minE/1138804‑1138928

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 21 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`