| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,139,906 | T→C | 100% | L428L (TTA→CTA) | ydjN → | predicted transporter |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,139,906 | 0 | T | C | 76.0% | 33.2 / 5.7 | 29 | L428L (TTA→CTA) | ydjN | predicted transporter |
| Reads supporting (aligned to +/- strand): ref base T (0/7); new base C (22/0); total (22/7) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.41e-07 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.16e-01 | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
CTGCCAGTAACCCTGGTGGCGCTGTTAATCTCCGTTGAACCGCTTATCGACATGGGCCGTACGGCGTTAAACGTTAGTGGCTCGATGACAGCTGGCACGCTGACCAGCCAGTGGCTGAAGCAAA > minE/1139840‑1139963 | cTGCCAGTAACCCTGGTGGCGCTGTTAATCTCCGTTGAACCGCTTATCGACATGGGCCGTACGGCGTTa < 1:960776/69‑1 (MQ=255)cTGCCAGTAACCCTGGTGGCGCTGTTAATCTCCGTTGAACCGCTTATCGACATGGGCCGTACGGCGTTa < 1:3204059/69‑1 (MQ=255) aGTAACCCTGGTGGCGCTGTTAATCTCCGTTGAACCGCTTATCGACATGGGCCGTACGGCGTTAAACGt < 1:1294904/69‑1 (MQ=255) aGTAACCCTGGTGGCGCTGTTAATCTCCGTTGAACCGCTTATCGACATGGGCCGTACGGCGTTAAACGt < 1:180550/69‑1 (MQ=255) aGTAACCCTGGTGGCGCTGTTAATCTCCGTTGAACCGCTTATCGACATGGGCCGTACGGCGTTAAACGt < 1:2302603/69‑1 (MQ=255) aGTAACCCTGGTGGCGCTGTTAATCTCCGTTGAACCGCTTATCGACATGGGCCGTACGGCGTTAAACGt < 1:2466174/69‑1 (MQ=255) ccTGGTGGCGCTGTTAATCTCCGTTGAACCGCTTATCGACATGGGCCGTACGGCGTTa < 1:2271445/58‑1 (MQ=255) tGTTAATCTCCGTTGAACCGCTTATCGACATGGGTCGTACGGCGCTAAACGTTAGTGGCTCGa > 1:1404525/1‑63 (MQ=255) tGTTAATCTCCGTTGAACCGCTTATCGACATGGGTCGTACGGCGCTAAACGTTAGTGGCTCGAt > 1:1094070/1‑64 (MQ=255) tGTTAATCTCCGTTGAACCGCTTATCGACATGGGTCGTACGGCGCTAAACGTTAGTGGCTCGAt > 1:1264685/1‑64 (MQ=255) tGTTAATCTCCGTTGAACCGCTTATCGACATGGGTCGTACGGCGCTAAACGTTAGTGGCTCGAt > 1:333586/1‑64 (MQ=255) tGTTAATCTCCGTTGAACCGCTTATCGACATGGGTCGTACGGCGCTAAACGTTAGTGGCTCGAt > 1:1671642/1‑64 (MQ=255) tGTTAATCTCCGTTGAACCGCTTATCGACATGGGTCGTACGGCGCTAAACGTTAGTGGCTCGAt > 1:3072063/1‑64 (MQ=255) tGTTAATCTCCGTTGAACCGCTTATCGACATGGGTCGTACGGCGCTAAACGTTAGTGGCTCGAt > 1:2978059/1‑64 (MQ=255) tGTTAATCTCCGTTGAACCGCTTATCGACATGGGTCGTACGGCGCTAAACGTTAGTGGCTCGAt > 1:2932763/1‑64 (MQ=255) gtcGTACGGCGCTAAACGTTAGTGGCTCGATGACCGCTGGCACGCTGAccag > 1:123257/3‑52 (MQ=255) gtcGTACGGCGCTAAACGTTAGTGGCTCGATGACCGCTGGCACGCTGACCAGCCAGTGGCTGAAGCaaa > 1:2715353/3‑69 (MQ=255) gtcGTACGGCGCTAAACGTTAGTGGCTCGATGACCGCTGGCACGCTGACCAGCCAGTGGCTGAAGCaaa > 1:269490/3‑69 (MQ=255) gtcGTACGGCGCTAAACGTTAGTGGCTCGATGACCGCTGGCACGCTGACCAGCCAGTGGCTGAAGCaaa > 1:2489512/3‑69 (MQ=255) gtcGTACGGCGCTAAACGTTAGTGGCTCGATGACCGCTGGCACGCTGACCAGCCAGTGGCTGAAGCaaa > 1:3011513/3‑69 (MQ=255) gtcGTACGGCGCTAAACGTTAGTGGCTCGATGACCGCTGGCACGCTGACCAGCCAGTGGCTGAAGCaaa > 1:1917842/3‑69 (MQ=255) gtcGTACGGCGCTAAACGTTAGTGGCTCGATGACCGCTGGCACGCTGACCAGCCAGTGGCTGAAGCaaa > 1:3104079/3‑69 (MQ=255) gtcGTACGGCGCTAAACGTTAGTGGCTCGATGACCGCTGGCACGCTGACCAGCCAGTGGCTGAAGCaaa > 1:3105497/3‑69 (MQ=255) gtcGTACGGCGCTAAACGTTAGTGGCTCGATGACCGCTGGCACGCTGACCAGCCAGTGGCTGAAGCaaa > 1:1502920/3‑69 (MQ=255) gtcGTACGGCGCTAAACGTTAGTGGCTCGATGACCGCTGGCACGCTGACCAGCCAGTGGCTGAAGCaaa > 1:1469723/3‑69 (MQ=255) gtcGTACGGCGCTAAACGTTAGTGGCTCGATGACCGCTGGCACGCTGACCAGCCAGTGGCTGAAGCaaa > 1:3352660/3‑69 (MQ=255) gtcGTACGGCGCTAAACGTTAGTGGCTCGATGACCGCTGGCACGCTGACCAGCCAGTGGCTGAAGCaaa > 1:890815/3‑69 (MQ=255) gtcGTACGGCGCTAAACGTTAGTGGCTCGATGACCGCTGGCACGCTGACCAGCCAGTGGCTGAAGCaa > 1:2985455/3‑68 (MQ=255) gtcGTACGGCGCTAAACGTTAGTGGCTCGATGACCGCTGGCACGCTGACCAGCCAGTGGCTGAAGCa > 1:1461016/3‑67 (MQ=255) | CTGCCAGTAACCCTGGTGGCGCTGTTAATCTCCGTTGAACCGCTTATCGACATGGGCCGTACGGCGTTAAACGTTAGTGGCTCGATGACAGCTGGCACGCTGACCAGCCAGTGGCTGAAGCAAA > minE/1139840‑1139963 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |