| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,143,119 | G→A | 39.9% | R521H (CGC→CAC) | katE → | hydroperoxidase HPII(III) |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,143,119 | 0 | G | A | 39.9% | 54.7 / 74.8 | 111 | R521H (CGC→CAC) | katE | hydroperoxidase HPII(III) |
| Reads supporting (aligned to +/- strand): ref base G (27/39); new base A (19/25); total (46/65) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 8.46e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
Only 100 of 117 total aligned reads displayed.
CCATCGTTTGGCGAATATTATTCCCATCCGCGTCTGTTCTGGCTAAGTCAGACGCCATTTGAGCAGCGCCATATTGTCGATGGTTTCAGTTTTGAGTTAAGCAAAGTCGTTCGTCCGTATATTCGTGAG > minE/1143052‑1143180 | ccATCGTTTGGCGAATATTATTCCCATCCGCGTCTGTTCTGGCTAAGTCAGACGCCATTTGAGCAgcgc < 1:2537164/69‑1 (MQ=255)ccATCGTTTGGCGAATATTATTCCCATCCGCGTCTGTTCTGGCTAAGTCAGACGCCATTTGAGCAgcgc < 1:2341061/69‑1 (MQ=255)ccATCGTTTGGCGAATATTATTCCCATCCGCGTCTGTTCTGGCTAAGTCAGACGCCATTTGAGAAgcgc < 1:1155322/69‑1 (MQ=255) gTTTGGCGAATATTATTCCCATCCGCGTCTGTTCTGGCTAAGTCAGACGCCATTTGAGCAGCGCCatat > 1:2700763/1‑69 (MQ=255) gTTTGGCGAATATTATTCCCATCCGCGTCTGTTCTGGCTAAGTCAGACGCCATTTGAGCAGCGCCatat > 1:40254/1‑69 (MQ=255) tattatTCCCATCCGCGTCTGTTCTGGCTAAGTCAGACGCCATTTGAGCAgcg > 1:3064977/1‑53 (MQ=255) tattatTCCCATCCGCGTCTGTTCTGGCTAAGTCAGACGCCATTTGAGCAGCGCCATATTGTCGATgg > 1:3217062/1‑68 (MQ=255) tattatTCCCATCCGCGTCTGTTCTGGCTAAGTCAGACGCCATTTGAGCAGCGCCATATTGTCGATgg > 1:2813218/1‑68 (MQ=255) tattatTCCCATCCGCGTCTGTTCTGGCTAAGTCAGACGCCATTTGAGCAGCGCCATATTGTCGATgg > 1:2783132/1‑68 (MQ=255) tattatTCCCATCCGCGTCTGTTCTGGCTAAGTCAGACGCCATTTGAGCAGCGCCATATTGTCGATgg > 1:807149/1‑68 (MQ=255) tattatTCCCATCCGCGTCTGTTCTGGCTAAGTCAGACGCCATTTGAGCAGCGCCATATTGTCGATgg > 1:2216727/1‑68 (MQ=255) tattatTCCCATCCGCGTCTGTTCTGGCTAAGTCAGACGCCATTTGAGCAGCGCCATATTGTCGATgg > 1:2266351/1‑68 (MQ=255) tattatTCCCATCCGCGTCTGTTCTGGCTAAGTCAGACGCCATTTGAGCAGCGCCATATTGTCGATgg > 1:163921/1‑68 (MQ=255) tattatTCCCATCCGCGTCTGTTCTGGCTAAGTCAGACGCCATTTGAGCAGCGCCATATTGTCGATGGt > 1:635229/1‑69 (MQ=255) tattatTCCCATCCGCGTCTGTTCTGGCTAAGTCAGACGCCATTTGAGCAGCGCCATATTGTCGATGGt > 1:229569/1‑69 (MQ=255) tattatTCCCATCCGCGTCTGTTCTGGCTAAGTCAGACGCCATTTGAGCAGCGCCATATTGTCGATGGt > 1:1947842/1‑69 (MQ=255) tattatTCCCATCCGCGTCTGTTCTGGCTAAGTCAGACGCCATTTGAGCAGCGCCATATTGTCGATGGt > 1:253326/1‑69 (MQ=255) tattatTCCCATCCGCGTCTGTTCTGGCTAAGTCAGACGCCATTTGAGCAGCGCCATATTGTCGATGGt > 1:2565127/1‑69 (MQ=255) tattatTCCCATCCGCGTCTGTTCTGGCTAAGTCAGACGCCATTTGAGCAGCGCCATATTGTCGATGGt > 1:2581087/1‑69 (MQ=255) tattatTCCCATCCGCGTCTGTTCTGGCTAAGTCAGACGCCATTTGAGCAGCGCCATATTGTCGATGGt > 1:3298473/1‑69 (MQ=255) tattatTCCCATCCGCGTCTGTTCTGGCTAAGTCAGACGCCATTTGAGCAGCGCCATATTGTCGATGGt > 1:870498/1‑69 (MQ=255) attCCCATCCGCGTCTGTTCTGGCTAAGTCAGACGCCATTTGAGCAGCGCCATATTGTCGATGGTTTCa > 1:2950620/1‑69 (MQ=255) attCCCATCCGCGTCTGTTCTGGCTAAGTCAGACGCCATTTGAGCAGCGCCATATTGTCGATGGTTTCa > 1:740018/1‑69 (MQ=255) attCCCATCCGCGTCTGTTCTGGCTAAGTCAGACGCCATTTGAGCAGCGCCATATTGTCGATGGTTTCa > 1:2389738/1‑69 (MQ=255) tCCCATCCGCGTCTGTTCTGGCTAAGTCAGACGCCATTTGAGCAGCGCCATATTGTCGATGGTTTCAg < 1:2951604/68‑1 (MQ=255) tCCCATCCGCGTCTGTTCTGGCTAAGTCAGACGCCATTTGAGCAGCGCCATATTGTCGATGGTTTCAg < 1:1284745/68‑1 (MQ=255) tCCCATCCGCGTCTGTTCTGGCTAAGTCAGACGCCATTTGAGCAGCGCCATATTGTCGATGGTTTCAg < 1:2683854/68‑1 (MQ=255) tCCCATCCGCGTCTGTTCTGGCTAAGTCAGACGCCATTTGAGCAGCGCCATATTGTCGATGGTTTCAg < 1:2632795/68‑1 (MQ=255) tCCCATCCGCGTCTGTTCTGGCTAAGTCAGACGCCATTTGAGCAGCGCCATATTGTCGATGGTTTCAg < 1:1370717/68‑1 (MQ=255) tCCCATCCGCGTCTGTTCTGGCTAAGTCAGACGCCATTTGAGCAGCGCCATATTGTCGATGGTTTCAg < 1:2561276/68‑1 (MQ=255) tCCCATCCGCGTCTGTTCTGGCTAAGTCAGACGCCATTTGAGCAGCGCCATATTGTCGATGGTTTCAg < 1:801526/68‑1 (MQ=255) cccATCCGCGTCTGTTCTGGCTAAGTCAGACGCCATTTGAGCAGCGCCATATTGTCGATGGTTTCAg < 1:2622827/67‑1 (MQ=255) cccATCCGCGTCTGTTCTGGCTAAGTCAGACGCCATTTGAGCAGCGCCATATTGTCGATGGTTTCAg < 1:2222745/67‑1 (MQ=255) tGTTCTGGCTAAGTCAGACGCCATTTGAGCAGCGCCATATTGTCGATGGTTTCAGTTTTGAGTTAAg > 1:873879/1‑67 (MQ=255) tGTTCTGGCTAAGTCAGACGCCATTTGAGCAGCGCCATATTGTCGATGGTTTCAGTTTTGAGTTAAg > 1:1174258/1‑67 (MQ=255) tGTTCTGGCTAAGTCAGACGCCATTTGAGCAGCGCCATATTGTCGATGGTTTCAGTTTTGAGTTAAg > 1:417626/1‑67 (MQ=255) tGTTCTGGCTAAGTCAGACGCCATTTGAGCAGCGCCATATTGTCGATGGTTTCAGTTTTGAGTTAAGCa < 1:2451664/69‑1 (MQ=255) tGTTCTGGCTAAGTCAGACGCCATTTGAGCAGCGCCATATTGTCGATGGTTTCAGTTTTGAGTTAAGCa < 1:431614/69‑1 (MQ=255) tGTTCTGGCTAAGTCAGACGCCATTTGAGCAGCGCCATATTGTCGATGGTTTCAGTTTTGAGTTAAGCa < 1:1250319/69‑1 (MQ=255) tGTTCTGGCTAAGTCAGACGCCATTTGAGCAGCGCCATATTGTCGATGGTTTCAGTTTTGAGTTAAGCa < 1:2902410/69‑1 (MQ=255) tGTTCTGGCTAAGTCAGACGCCATTTGAGCAGCGCCATATTGTCGATGGTTTCAGTTTTGAGTTAAGCa < 1:2589840/69‑1 (MQ=255) tGTTCTGGCTAAGTCAGACGCCATTTGAGCAGCGCCATATTGTCGATGGTTTCAGTTTTGAGTTAAGCa < 1:3416584/69‑1 (MQ=255) tGTTCTGGCTAAGTCAGACGCAATTTGAGCAGCGCCATATTGTCGATGGTTTCAGTTTTGAGTTAAGCa < 1:793092/69‑1 (MQ=255) cTGGCTAAGTCAGACGCCATTTGAGCAGCGCCATATTGTCGATGGTTTCAGTTTTGAGTTAAGCAAAg < 1:311083/68‑1 (MQ=255) gCTAAGTCAGTCGCCATTCGAGCAGCACCATATTGTCGATGGTTTCAGtt < 1:17896/50‑1 (MQ=255) gCTAAGTCAGACGCCATTCGAGCAGCAGCATATTGTCGATGGTTTCAGtt < 1:38409/50‑1 (MQ=255) gCTAAGTCAGACGCCATTCGAGCAGCACCATATTGTCGATGGTTTCAGtt < 1:1894150/50‑1 (MQ=255) gCTAAGTCAGACGCCATTCGAGCAGCACCATATTGTCGATGGTTTCAGtt < 1:656403/50‑1 (MQ=255) gCTAAGTCAGACGCCATTCGAGCAGCACCATATTGTCGATGGTTTCAGtt < 1:1235471/50‑1 (MQ=255) gCTAAGTCAGACGCCATTCGAGCAGCACCATATTGTCGATGGTTTCAGtt < 1:2885713/50‑1 (MQ=255) gCTAAGTCAGACGCCATTCGAGCAGCACCATATTGTCGATGGTTTCAGtt < 1:2934753/50‑1 (MQ=255) gCTAAGTCAGACGCCATTCGAGCAGCACCATATTGTCGATGGTTTCAGtt < 1:3157006/50‑1 (MQ=255) gCTAAGTCAGACGCCATTCGAGCAGCACCATATTGTCGATGGTTTCAGtt < 1:1758702/50‑1 (MQ=255) gCTAAGTCAGACGCCATTCGAGCAGCACCATATTGTCGATGGTTTCAGtt < 1:3345524/50‑1 (MQ=255) gCTAAGTCAGACGCCATTCGAGCAGCACCATATTGTCGATGGTTTCAGtt < 1:1929365/50‑1 (MQ=255) gCTAAGTCAGACGCCATTCGAGCAGCACCATATTGTCGATGGTTTCAGtt < 1:1939018/50‑1 (MQ=255) gCTAAGTCAGACGCCATTCGAGCAGCACCATATTGTCGATGGTTTCAGtt < 1:1684879/50‑1 (MQ=255) gCTAAGTCAGACGCCATTCGAGCAGCACCATATTGTCGATGGTTTCAGtt < 1:484206/50‑1 (MQ=255) gCTAAGTCAGACGCCATTCGAGCAGCACCATATTGTCGATGGTTTCAGtt < 1:1424844/50‑1 (MQ=255) gCTAAGTCAGACGCCATTCGAGCAGCACCATATTGTCGATGGTTTCAGtt < 1:736368/50‑1 (MQ=255) gCTAAGTCAGACGCCATTCGAGCAGCACCATATTGTCGATGGTTTCAGtt < 1:1604146/50‑1 (MQ=255) gCTAAGTCAGACGCCATTCGAGCAGCACCATATTGTCGATGGTTTCAGtt < 1:791830/50‑1 (MQ=255) cTACGTCAGACGCCATTCGAGCAGCACCATATTGTCGATGGTTTGAGtt < 1:381505/49‑1 (MQ=255) aGTCAGACGCCATTTGAGCAGCGCCATATTGTCGATGGTTTCAg < 1:1901746/44‑1 (MQ=255) tCAGACGCCATTTGAGCAGCGCCATATTGTCGATGGTTTCAGTTTTGAGTTAAGCAAAGTCGTTCGTc > 1:1842647/1‑68 (MQ=255) tCAGACGCCATTTGAGCAGCGCCATATTGTCGATGGTTTCAGTTTTGAGTTAAGCAAAGTCGTTCGTc > 1:2970309/1‑68 (MQ=255) tCAGACGCCATTCGAGCAGCACCATATTGTCGATGGTTTCAGtt < 1:1139671/44‑1 (MQ=38) aCGCCATTTGAGCAGCGCCATATTGTCGATGGTTTCAGTTTTGAGTTAAGCAAAGTCGTTCGTCCGta < 1:929512/68‑1 (MQ=255) aCGCCATTTGAGCAGCGCCATATTGTCGATGGTTTCAGTTTTGAGTTAAGCAAAGTCGTTCGTCCGta < 1:874494/68‑1 (MQ=255) aCGCCATTTGAGCAGCGCCATATTGTCGATGGTTTCAGTTTTGAGTTAAGCAAAGTCGTTCGTCCGta < 1:1022247/68‑1 (MQ=255) aCGCCATTTGAGCAGCGCCATATTGTCGATGGTTTCAGTTTTGAGTTAAGCAAAGTCGTTCGTCCGta < 1:1651229/68‑1 (MQ=255) aCGCCATTTGAGCAGCGCCATATTGTCGATGGTTTCAGTTTTGAGTTAAGCAAAGTCGTTCGTCCGta < 1:3248791/68‑1 (MQ=255) aCGCCATTTGAGCAGCGCCATATTGTCGATGGTTTCAGTTTTGAGTTAAGCAAAGTCGTTCGTCCGta < 1:1656830/68‑1 (MQ=255) aCGCCATTTGAGCAGCGCCATATTGTCGATGGTTTCAGTTTTGAGTTAAGCAAAGTCGTTCGTCCGta < 1:337695/68‑1 (MQ=255) aCGCCATTTGAGCAGCGCCATATTGTCGATGGTTTCAGTTTTGAGTTAAGCAAAGTCGTTCGTCCGta < 1:1693268/68‑1 (MQ=255) aCGCCATTTGAGCAGCGCCATATTGTCGATGGTTTCAGTTTTGAGTTAAGCAAAGTCGTTCGTCCGta < 1:2430493/68‑1 (MQ=255) cGCCATTTGAGCAGCGCCATATTGTCGATGGTTTCAGTTTTGAGTTAAGCAAAGTCGTTCGTCCGtata < 1:3011654/69‑1 (MQ=255) cGCCATTTGAGCAGCGCCATATTGTCGATGGTTTCAGTTTTGAGTTAAGCAAAGTCGTTCGTCCGtata < 1:658373/69‑1 (MQ=255) cGCCATTTGAGCAGCGCCATATTGTCGATGGTTTCAGTTTTGAGTTAAGCAAAGTCGTTCGTCCGtata < 1:535735/69‑1 (MQ=255) cGCCATTTGAGCAGCGCCATATTGTCGATGGTTTCAGTTTTGAGTTAAGCAAAGTCGTTCGTCCGtata < 1:426144/69‑1 (MQ=255) cGCCATTTGAGCAGCGCCATATTGTCGATGGTTTCAGTTTTGAGTTAAGCAAAGTCGTTCGTCCGtata < 1:2941427/69‑1 (MQ=255) cGCCATTTGAGCAGCGCCATATTGTCGATGGTTTCAGTTTTGAGTTAAGCAAAGTCGTTCGTCCGtata < 1:2780022/69‑1 (MQ=255) cGCCATTTGAGCAGCGCCATATTGTCGATGGTTTCAGTTTTGAGTTAAGCAAAGTCGTTCGTCCGtata < 1:2855660/69‑1 (MQ=255) cGCCATTTGAGCAGCCCCATATTGTCGATGGTTTCAGTTTTGAGTTAAGCAAAGTCGTTCGTCCGtata < 1:2466645/69‑1 (MQ=255) cgAGCAGGACCATATTGTCGATGGTTTCAGTTTTGAGTTAAGCAAAGTAGTTCGTCCGTATATTCGTGa > 1:3082105/2‑69 (MQ=255) cgAGCAGCACCATATTGTCGATGGTTTCAGTTTTGAGTTAAGCAAAGTAGTTCGTCCGTATATTCGTGa > 1:2932065/2‑69 (MQ=255) cgAGCAGCACCATATTGTCGATGGTTTCAGTTTTGAGTTAAGCAAAGTAGTTCGTCCGTATATTCGTGa > 1:892593/2‑69 (MQ=255) cgAGCAGCACCATATTGTCGATGGTTTCAGTTTTGAGTTAAGCAAAGTAGTTCGTCCGTATATTCGTGa > 1:3117094/2‑69 (MQ=255) cgAGCAGCACCATATTGTCGATGGTTTCAGTTTTGAGTTAAGCAAAGTAGTTCGTCCGTATATTCGTGa > 1:1270479/2‑69 (MQ=255) cgAGCAGCACCATATTGTCGATGGTTTCAGTTTTGAGTTAAGCAAAGTAGTTCGTCCGTATATTCGTGa > 1:1562366/2‑69 (MQ=255) cgAGCAGCACCATATTGTCGATGGTTTCAGTTTTGAGTTAAGCAAAGTAGTTCGTCCGTATATTCGTGa > 1:157573/2‑69 (MQ=255) cgAGCAGCACCATATTGTCGATGGTTTCAGTTTTGAGTTAAGCAAAGTAGTTCGTCCGTATATTCGTGa > 1:1642074/2‑69 (MQ=255) cgAGCAGCACCATATTGTCGATGGTTTCAGTTTTGAGTTAAGCAAAGTAGTTCGTCCGTATATTCGTGa > 1:1700764/2‑69 (MQ=255) cgAGCAGCACCATATTGTCGATGGTTTCAGTTTTGAGTTAAGCAAAGTAGTTCGTCCGTATATTCGTGa > 1:542446/2‑69 (MQ=255) cgAGCAGCACCATATTGTCGATGGTTTCAGTTTTGAGTTAAGCAAAGTAGTTCGTCCGTATATTCGTGa > 1:2580949/2‑69 (MQ=255) cgAGCAGCACCATATTGTCGATGGTTTCAGTTTTGAGTTAAGCAAAGTAGTTCGTCCGTATATTCGTGa > 1:2110534/2‑69 (MQ=255) cgAGCAGCACCATATTGTCGATGGTTTCAGTTTTGAGTTAAGCAAAGTAGTTCGTCCGTATATTCGTGa > 1:2508427/2‑69 (MQ=255) cgAGCAGCACCATATTGTCGATGGTTTCAGTTTTGAGTTAAGCAAAGTAGTTCGTCCGTATATTCGTGa > 1:2507319/2‑69 (MQ=255) agcagcACCATATTGTCGATGGTTTCAGTTTTGAGTTAAGCAAAGTAGTTCGTCCGTATATTCGTGAg < 1:2631239/68‑1 (MQ=255) agcagcACCATATTGTCGATGGTTTCAGTTTTGAGTTAAGCAAAGTAGTTCGTCCGTATATTCGTGAg < 1:1359999/68‑1 (MQ=255) | CCATCGTTTGGCGAATATTATTCCCATCCGCGTCTGTTCTGGCTAAGTCAGACGCCATTTGAGCAGCGCCATATTGTCGATGGTTTCAGTTTTGAGTTAAGCAAAGTCGTTCGTCCGTATATTCGTGAG > minE/1143052‑1143180 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |