| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,143,159 | C→A | 24.5% | V534V (GTC→GTA) | katE → | hydroperoxidase HPII(III) |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,143,159 | 0 | C | A | 24.5% | 216.6 / 55.9 | 126 | V534V (GTC→GTA) | katE | hydroperoxidase HPII(III) |
| Reads supporting (aligned to +/- strand): ref base C (24/71); new base A (28/3); total (52/74) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.02e-10 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.65e-01 | |||||||||||
Only 100 of 127 total aligned reads displayed.
TCAGACGCCATTTGAGCAGCGCCATATTGTCGATGGTTTCAGTTTTGAGTTAAGCAAAGTCGTTCGTCCGTATATTCGTGAGCGCGTTGTTGACCAGCTGGCGCATATTGATCTCACTCTGGCCCA > minE/1143099‑1143224 | tCAGACGCCATTTGAGCAGCGCCATATTGTCGATGGTTTCAGTTTTGAGTTAAGCAAAGTCGTTCGTc > 1:1842647/1‑68 (MQ=255)tCAGACGCCATTTGAGCAGCGCCATATTGTCGATGGTTTCAGTTTTGAGTTAAGCAAAGTCGTTCGTc > 1:2970309/1‑68 (MQ=255) aCGCCATTTGAGCAGCGCCATATTGTCGATGGTTTCAGTTTTGAGTTAAGCAAAGTCGTTCGTCCGta < 1:1022247/68‑1 (MQ=255) aCGCCATTTGAGCAGCGCCATATTGTCGATGGTTTCAGTTTTGAGTTAAGCAAAGTCGTTCGTCCGta < 1:3248791/68‑1 (MQ=255) aCGCCATTTGAGCAGCGCCATATTGTCGATGGTTTCAGTTTTGAGTTAAGCAAAGTCGTTCGTCCGta < 1:1693268/68‑1 (MQ=255) aCGCCATTTGAGCAGCGCCATATTGTCGATGGTTTCAGTTTTGAGTTAAGCAAAGTCGTTCGTCCGta < 1:1651229/68‑1 (MQ=255) aCGCCATTTGAGCAGCGCCATATTGTCGATGGTTTCAGTTTTGAGTTAAGCAAAGTCGTTCGTCCGta < 1:874494/68‑1 (MQ=255) aCGCCATTTGAGCAGCGCCATATTGTCGATGGTTTCAGTTTTGAGTTAAGCAAAGTCGTTCGTCCGta < 1:2909338/68‑1 (MQ=255) aCGCCATTTGAGCAGCGCCATATTGTCGATGGTTTCAGTTTTGAGTTAAGCAAAGTCGTTCGTCCGta < 1:2430493/68‑1 (MQ=255) aCGCCATTTGAGCAGCGCCATATTGTCGATGGTTTCAGTTTTGAGTTAAGCAAAGTCGTTCGTCCGta < 1:929512/68‑1 (MQ=255) cGCCATTTGAGCAGCGCCATATTGTCGATGGTTTCAGTTTTGAGTTAAGCAAAGTCGTTCGTCCGtata < 1:658373/69‑1 (MQ=255) cGCCATTTGAGCAGCGCCATATTGTCGATGGTTTCAGTTTTGAGTTAAGCAAAGTCGTTCGTCCGtata < 1:3011654/69‑1 (MQ=255) cGCCATTTGAGCAGCGCCATATTGTCGATGGTTTCAGTTTTGAGTTAAGCAAAGTCGTTCGTCCGtata < 1:535735/69‑1 (MQ=255) cGCCATTTGAGCAGCGCCATATTGTCGATGGTTTCAGTTTTGAGTTAAGCAAAGTCGTTCGTCCGtata < 1:426144/69‑1 (MQ=255) cGCCATTTGAGCAGCCCCATATTGTCGATGGTTTCAGTTTTGAGTTAAGCAAAGTCGTTCGTCCGtata < 1:2466645/69‑1 (MQ=255) cgAGCAGCACCATATTGTCGATGGTTTCAGTTTTGAGTTAAGCAAAGTAGTTCGTCCGTATATTCGTGa > 1:2932065/2‑69 (MQ=255) cgAGCAGCACCATATTGTCGATGGTTTCAGTTTTGAGTTAAGCAAAGTAGTTCGTCCGTATATTCGTGa > 1:2110534/2‑69 (MQ=255) cgAGCAGCACCATATTGTCGATGGTTTCAGTTTTGAGTTAAGCAAAGTAGTTCGTCCGTATATTCGTGa > 1:3117094/2‑69 (MQ=255) cgAGCAGCACCATATTGTCGATGGTTTCAGTTTTGAGTTAAGCAAAGTAGTTCGTCCGTATATTCGTGa > 1:3165068/2‑69 (MQ=255) cgAGCAGCACCATATTGTCGATGGTTTCAGTTTTGAGTTAAGCAAAGTAGTTCGTCCGTATATTCGTGa > 1:1700764/2‑69 (MQ=255) cgAGCAGCACCATATTGTCGATGGTTTCAGTTTTGAGTTAAGCAAAGTAGTTCGTCCGTATATTCGTGa > 1:2580949/2‑69 (MQ=255) cgAGCAGCACCATATTGTCGATGGTTTCAGTTTTGAGTTAAGCAAAGTAGTTCGTCCGTATATTCGTGa > 1:2507319/2‑69 (MQ=255) cgAGCAGCACCATATTGTCGATGGTTTCAGTTTTGAGTTAAGCAAAGTAGTTCGTCCGTATATTCGTGa > 1:1642074/2‑69 (MQ=255) cgAGCAGCACCATATTGTCGATGGTTTCAGTTTTGAGTTAAGCAAAGTAGTTCGTCCGTATATTCGTGa > 1:157573/2‑69 (MQ=255) cgAGCAGCACCATATTGTCGATGGTTTCAGTTTTGAGTTAAGCAAAGTAGTTCGTCCGTATATTCGTGa > 1:1562366/2‑69 (MQ=255) cgAGCAGCACCATATTGTCGATGGTTTCAGTTTTGAGTTAAGCAAAGTAGTTCGTCCGTATATTCGTGa > 1:1270479/2‑69 (MQ=255) agcagcACCATATTGTCGATGGTTTCAGTTTTGAGTTAAGCAAAGTAGTTCGTCCGTATATTCGTGAg < 1:2631239/68‑1 (MQ=255) ccATATTGTCGATGGTTTCAGTTTTGAGTTAAGCAAAGTAGTTCGTCCGTATATTCGTGAg < 1:2618819/61‑1 (MQ=255) ttGTCGATGGTTTCAGTTTTGAGTTTAGCAAAGTCGTTCGTCCGTATATTCGTGAGCGCGTTGTTGAcc > 1:2179097/1‑69 (MQ=255) ttGTCGATGGTTTCAGTTTTGAGTTAAGCAAAGtcgt > 1:2993116/1‑37 (MQ=255) ttGTCGATGGTTTCAGTTTTGAGTTAAGCAAAGTCGTTCGTCCGTATATTCGTGAGCGCGTTGTTGAcc > 1:3170503/1‑69 (MQ=255) ttGTCGATGGTTTCAGTTTTGAGTTAAGCAAAGTCGTTCGTCCGTATATTCGTGAGCGCGTTGTTGAcc > 1:971253/1‑69 (MQ=255) ttGTCGATGGTTTCAGTTTTGAGTTAAGCAAAGTCGTTCGTCCGTATATTCGTGAGCGCGTTGTTGAcc > 1:1151412/1‑69 (MQ=255) ttGTCGATGGTTTCAGTTTTGAGTTAAGCAAAGTCGTTCGTCCGTATATTCGTGAGCGCGTTGTTGAcc > 1:905298/1‑69 (MQ=255) ttGTCGATGGTTTCAGTTTTGAGTTAAGCAAAGTCGTTCGTCCGTATATTCGTGAGCGCGTTGTTGAcc > 1:2842574/1‑69 (MQ=255) ttGTCGATGGTTTCAGTTTTGAGTTAAGCAAAGTCGTTCGTCCGTATATTCGTGAGCGCGTTGTTGAcc > 1:892520/1‑69 (MQ=255) ttGTCGATGGTTTCAGTTTTGAGTTAAGCAAAGTCGTTCGTCCGTATATTCGTGAGCGCGTTGTTGAcc > 1:2174661/1‑69 (MQ=255) ttGTCGATGGTTTCAGTTTTGAGTTAAGCAAAGTCGTTCGTCCGTATATTCGTGAGCGCGTTGTTGAcc > 1:883464/1‑69 (MQ=255) ttGTCGATGGTTTCAGTTTTGAGTTAAGCAAAGTCGTTCGTCCGTATATTCGTGAGCGCGTTGTTGAcc > 1:3052873/1‑69 (MQ=255) ttGTCGATGGTTTCAGTTTTGAGTTAAGCAAAGTCGTTCGTCCGTATATTCGTGAGCGCGTTGTTGAcc > 1:483889/1‑69 (MQ=255) ttGTCGATGGTTTCAGTTTTGAGTTAAGCAAAGTCGTTCGTCCGTATATTCGTGAGCGCGTTGTTGAcc > 1:1667122/1‑69 (MQ=255) ttGTCGATGGTTTCAGTTTTGAGTTAAGCAAAGTCGTTCGTCCGTATATTCGTGAGCGCGTTGTTGAc > 1:1370171/1‑68 (MQ=255) ttGTCGATGGTTTCAGTTTTGAGTTAAGCAAAGTCGTTCGTCCGTATATTAGTGAGCGCGTTGTTGAcc > 1:289269/1‑69 (MQ=255) ttGACGATGGTTTCAGTTTTGAGTTAAGCAAAGTCGTTCGTCCGTATATTCGTGAGCGCGTTGTTGAcc > 1:1043200/1‑69 (MQ=255) gTCGATGGTTTCAGTTTTGAGTTAAGCAAAGTCGTTCGTCCGTATATTCGTGAGGGCGTTGTTGACCa < 1:1943051/68‑1 (MQ=255) gTCGATGGTTTCAGTTTTGAGTTAAGCAAAGTCGTTCGTCCGTATATTCGTGAGCGCGTTGTTGACCa < 1:1047731/68‑1 (MQ=255) gTCGATGGTTTCAGTTTTGAGTTAAGCAAAGTCGTTCGTCCGTATATTCGTGAGCGCGTTGTTGACCa < 1:3389230/68‑1 (MQ=255) gTCGATGGTTTCAGTTTTGAGTTAAGCAAAGTCGTTCGTCCGTATATTCGTGAGCGCGTTGTTGACCa < 1:2200955/68‑1 (MQ=255) gTCGATGGTTTCAGTTTTGAGTTAAGCAAAGTCGTTCGTCCGTATATTCGTGAGCGCGTTGTTGACCa < 1:584743/68‑1 (MQ=255) gTCGATGGTTTCAGTTTTGAGTTAAGCAAAGTCGTTCGTCCGTATATTCGTGAGCGCGTTGTTGACCa < 1:2899736/68‑1 (MQ=255) gTCGATGGTTTCAGTTTTGAGTTAAGCAAAGTCGTTCGTCCGTATATTCGTGAGCGCGTTGTTGACCa < 1:2281438/68‑1 (MQ=255) gTCGATGGTTTCAGTTTTGAGTTAAGCAAAGTCGTTCGTCCGTATATTCGTGAGCGCGTTGTTGACCa < 1:597292/68‑1 (MQ=255) gATGGTTTCAGTTTTGAGTTAAGCAAAGTCGTTCGTCCGTATATTCGTGAGCGCGTTGTTGACCAGCt < 1:2815784/68‑1 (MQ=255) gATGGTTTCAGTTTTGAGTTAAGCAAAGTCGTTCGTCCGTATATTCGTGAGCGCGTTGTTGACCAGCt < 1:2839918/68‑1 (MQ=255) gATGGTTTCAGTTTTGAGTTAAGCAAAGTCGTTCGTCCGTATATTCGTGAGCGCGTTGTTGACCAGCt < 1:2299288/68‑1 (MQ=255) gATGGTTTCAGTTTTGAGTTAAGCAAAGTCGTTCGTCCGTATATTCGTGAGCGCGTTGTTGACCAGCt < 1:1696058/68‑1 (MQ=255) gATGGTTTCAGTTTTGAGTTAAGCAAAGTCGTTCGTCCGTATATTCGTGAGCGCGTTGTTGACCAGCt < 1:3095930/68‑1 (MQ=255) gATGGTTTCAGTTTTGAGTTAAGCAAAGTCGTTCGTCCGTATATTCGTGAGCGCGTTGTTGACCAGCt < 1:346464/68‑1 (MQ=255) tttCAGTTTTGAGTTAAGCAAAGTCGTTCGTCCGTATATTCGTGAGCGCGTTGTTGACCa < 1:2421/60‑1 (MQ=255) tCAGTTTTGAGTTAAGCAAAGTCGTTCGTCCGTATATTCGTGAGCGCGTTGTTGACCAGCt < 1:280824/61‑1 (MQ=255) tCAGTTTTGAGTTAAGCAAAGTCGTTCGTCCGTATATTCGTGAGCGCGTTGTTGACCAGCTGGCGCat > 1:1633781/1‑68 (MQ=255) ttttGAGTTAAGCAAAGTCTTTCGTCCGTATATTCGTGAGCGCGTTGTTGACCAGCTGGCGCATATTg < 1:2404984/68‑1 (MQ=255) ttttGAGTTAAGCAAAGTCGTTCGTCCGTATATTCGTGAGCGCGTTGTTGACCAGCTGGCGCATATTg < 1:943641/68‑1 (MQ=255) ttttGAGTTAAGCAAAGTCGTTCGTCCGTATATTCGTGAGCGCGTTGTTGACCAGCTGGCGCATATTg < 1:1442439/68‑1 (MQ=255) ttttGAGTTAAGCAAAGTCGTTCGTCCGTATATTCGTGAGCGCGTTGTTGACCAGCTGGCGCATATTg < 1:856931/68‑1 (MQ=255) ttGAGTTAAGCAAAGTCGTTCGTCCGTATATTCGTGAGCGCGTTGTTGACCAGCTGGCGCATATTg < 1:2047572/66‑1 (MQ=255) gAGTTAAGCAAAGTCGTTCGTCCGTATATTCGTGAGCGCGTTGTTGACCAGCTGGCGCATATTGAtct < 1:583324/68‑1 (MQ=255) gAGTTAAGCAAAGTCGTTCGTCCGTATATTCGTGAGCGCGTTGTTGACCAGCTGGCGCATATTGAtct < 1:532080/68‑1 (MQ=255) gAGTTAAGCAAAGTCGTTCGTCCGTATATTCGTGAGCGCGTTGTTGACCAGCTGGCGCATATTGAtct < 1:2531194/68‑1 (MQ=255) gAGTTAAGCAAAGTCGTTCGTCCGTATATTCGTGAGCGCGTTGTTGACCAGCTGGCGCATATTGAtct < 1:2494332/68‑1 (MQ=255) gAGTTAAGCAAAGTAGTTCGTCCGTATATTCGTGa > 1:2569773/1‑35 (MQ=255) gAGTTAAGCAAAGTAGTTCGTCCGTATATTCGTGAGCGCGTTGTTGACCAGCt > 1:305818/1‑53 (MQ=255) gAGTTAAGCAAAGTAGTTCGTCCGTATATTCGTGAGCGCGTTGTTGACCAGCTg > 1:976470/1‑54 (MQ=255) gAGTTAAGCAAAGTAGTTCGTCCGTATATTCGTGAGCGCGTTGTTGACCAGCTg > 1:1859065/1‑54 (MQ=255) gAGTTAAGCAAAGTAGTTCGTCCGTATATTCGTGAGCGCGTTGTTGACCAGCTg > 1:3127479/1‑54 (MQ=255) gAGTTAAGCAAAGTAGTTCGTCCGTATATTCGTGAGCGCGTTGTTGACCAGCTg > 1:220715/1‑54 (MQ=255) gAGTTAAGCAAAGTAGTTCGTCCGTATATTCGTGAGCGCGTTGTTGACCAGCTg > 1:2477977/1‑54 (MQ=255) gAGTTAAGCAAAGTAGTTCGTCCGTATATTCGTGAGCGCGTTGTTGACCAGCTg > 1:2584660/1‑54 (MQ=255) gAGTTAAGCAAAGTAGTTCGTCCGTATATTCGTGAGCGCGTTGTTGACCAGCTg > 1:1580341/1‑54 (MQ=255) cAAAGTCGTTCGTCCGTATATTCGTGAGCGCGTTGTTGACCATCTGGCGCATATTGATCTCAc < 1:2942322/63‑1 (MQ=255) cAAAGTCGTTCGTCCGTATATTCGTGAGCGCGTTGTTGACCAGCTGGCGCATATTGATCTCAc < 1:719024/63‑1 (MQ=255) cAAAGTCGTTCGTCCGTATATTCGTGAGCGCGTTGTTGACCAGCTGGCGCATATTGATCTCAc < 1:591412/63‑1 (MQ=255) cAAAGTCGTTCGTCCGTATATTCGTGAGCGCGTTGTTGACCAGCTGGCGCATATTGATCTCAc < 1:1245971/63‑1 (MQ=255) cAAAGTCGTTCGTCCGTATATTCGTGAGCGCGTTGTTGACCAGCTGGCGCATATTGATCTCAc < 1:1224962/63‑1 (MQ=255) cAAAGTCGTTCGTCCGTATATTCGTGAGCGCGTTGTTGACCAGCTGGCGCATATTGATCTCAc < 1:936200/63‑1 (MQ=255) cAAAGTCGTTCGTCCGTATATTCGTGAGCGCGTTGTTGACCAGCTGGCGCATATTGATCTCAc < 1:969449/63‑1 (MQ=255) cAAAGTCGTTCGTCCGTATATTCGTGAGCGCGTTGTTGACCAGCTGGCGCATATTGATCTCAc < 1:3366457/63‑1 (MQ=255) cAAAGTCGTTCGTCCGTATATTCGTGAGCGCGTTGTTGACCAGCTGGCGCATATTGATCTCAc < 1:1824389/63‑1 (MQ=255) aGTCGTTCGTCCGTATATTCGTGAGCGCGTTGTTGACCAGCt < 1:1342828/42‑1 (MQ=255) aGTCGTTCGTCCGTATATTCGTGAGCGCGTTGTTGACCAGCTGGCGCATATTGATCTCACTCTGGCCCa < 1:1485863/69‑1 (MQ=255) aGTCGTTCGTCCGTATATTCGTGAGCGCGTTGTTGACCAGCTGGCGCATATTGATCTCACTCTGGCCCa < 1:2642511/69‑1 (MQ=255) aGTCGTTCGTCCGTATATTCGTGAGCGCGTTGTTGACCAGCTGGCGCATATTGATCTCACTCTGGCCCa < 1:767387/69‑1 (MQ=255) aGTCGTTCGTCCGTATATTCGTGAGCGCGTTGTTGACCAGCTGGCGCATATTGATCTCACTCTGGCCCa < 1:2968473/69‑1 (MQ=255) aGTCGTTCGTCCGTATATTCGTGAGCGCGTTGTTGACCAGCTGGCGCATATTGATCTCACTCTGGCCCa < 1:1470533/69‑1 (MQ=255) aGTCGTTCGTCCGTATATTCGTGAGCGCGTTGTTGACCAGCTGGCGCATATTGATCTCACTCTGGCCCa < 1:483967/69‑1 (MQ=255) aGTCGTTCGTCCGTATATTCGTGAGCGCGTTGTTGACCAGCTGGCGCATATTGATCTCACTCTGGCCCa < 1:1916178/69‑1 (MQ=255) aGTCGTTCGTCCGTATATTCGTGAGCGCGTTGTTGACCAGCTGGCGCATATTGATCTCACTCTGGCCCa < 1:1656619/69‑1 (MQ=255) aGTCGTTCGTCCGTATATTCGTGAGCGCGTTGTTGACCAGCTGGCGCATATTGATCTCACTCTGGCCCa < 1:3277276/69‑1 (MQ=255) aGTCGTTCGTCCGTATATTCGTGAGCGCGTTGTTGACCAGCTGGCGCATATTGATCTCACTCTGGCCCa < 1:1786928/69‑1 (MQ=255) gTCGTTCGTCCGTATATTCGTGAGCGCGTTGTTGACCAGCTGGCGCATATTGATCTCACTCTGGCCCa < 1:1874352/68‑1 (MQ=255) | TCAGACGCCATTTGAGCAGCGCCATATTGTCGATGGTTTCAGTTTTGAGTTAAGCAAAGTCGTTCGTCCGTATATTCGTGAGCGCGTTGTTGACCAGCTGGCGCATATTGATCTCACTCTGGCCCA > minE/1143099‑1143224 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |