| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,145,600 | A→G | 36.4% | L198L (TTG→CTG) | chbF ← | cryptic phospho‑beta‑glucosidase, NAD(P)‑binding |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,145,600 | 0 | A | G | 36.4% | 72.9 / 74.5 | 118 | L198L (TTG→CTG) | chbF | cryptic phospho‑beta‑glucosidase, NAD(P)‑binding |
| Reads supporting (aligned to +/- strand): ref base A (43/32); new base G (16/27); total (59/59) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.52e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.62e-01 | |||||||||||
Only 100 of 118 total aligned reads displayed.
GACTTGCCATTTATCAGCACATCCTTAATGAACACCATATGGTTGAGGCCGAACAAATCGATAGATAAATCATCGCTGTCTTTCAGCATCAGAACATCGCGAATAAACATCTT > minE/1145545‑1145657 | gACTTGCCATTTATCAGCACATCCTTAATGAACACCATATGGTTGAGGCCGAACAAATCGATAGATaa < 1:3038652/68‑1 (MQ=255) gCCATTTATCAGCACATCCTTAATGAACACCATATGGTTGAGGCCGAACAAATCGATAGATAAatcatc > 1:3112928/1‑69 (MQ=255) gCCATTTATCAGCACATCCTTAATGAACACCATATGGTTGAGGCCGAACAAATCGATAGATAAatcatc > 1:1450473/1‑69 (MQ=255) aTTTACCAGCACATCCTTAATGAACACCATATGGTTGAGGCCGAACAGATCGATAGATAAatca > 1:1005482/1‑64 (MQ=255) aTTTACCAGCACATCCTTAATGAACACCATATGGTTGAGGCCGAACAGATCGATAGATAAatca > 1:2128905/1‑64 (MQ=255) aTTTACCAGCACATCCTTAATGAACACCATATGGTTGAGGCCGAACAGATCGATAGATAAatca > 1:2134308/1‑64 (MQ=255) aTTTACCAGCACATCCTTAATGAACACCATATGGTTGAGGCCGAACAGATCGATAGATAAatca > 1:2323590/1‑64 (MQ=255) aTTTACCAGCACATCCTTAATGAACACCATATGGTTGAGGCCGAACAGATCGATAGATAAatca > 1:1476714/1‑64 (MQ=255) aTTTACCAGCACATCCTTAATGAACACCATATGGTTGAGGCCGAACAGATCGATAGATAAatca > 1:1418674/1‑64 (MQ=255) aTTTACCAGCACATCCTTAATGAACACCATATGGTTGAGGCCGAACAGATCGATAGATAAatca > 1:447004/1‑64 (MQ=255) aTTTACCAGCACATCCTTAATGAACACCATATGGTTGAGGCCGAACAGATCGATAGATAAatca > 1:631175/1‑64 (MQ=255) aTTTACCAGCACATCCTTAATGAACACCATATGGTTGAGGCCGAACAGATCGATAGATAAatca > 1:1223246/1‑64 (MQ=255) aTTTACCAGCACATCCTTAATGAACACCATATGGTTGAGGCCGAACAGATCGATAGATAAatca > 1:931717/1‑64 (MQ=255) aTTTACCAGCACATCCTTAATGAACACCATATGGTTGAGGCCGAACAGATCGATAGATAAatca > 1:1141731/1‑64 (MQ=255) aTTTACCAGCACATCCTTAATGAACACCATATGGTTGAGGCCGAACAGATAGATAGATAAatca > 1:2405779/1‑64 (MQ=255) aTTTACCAGCAAATCCTTAATGAACACCATATGGTTGAGGCCGAACAGATCGATAGATAAatca > 1:2980166/1‑64 (MQ=255) tATCAGCACATCCTTAATGAACACCATATGGTTGAGGCCGAACAAATCGATAGATAAATCATCGCTGTc > 1:2124396/1‑69 (MQ=255) cTTAATGAACACCATATGGTTGAGGCCGAACAAATCGATAGATAAATCATCGCTGTCTTTCAGCATCAg < 1:791823/69‑1 (MQ=255) cTTAATGAACACCATATGGTTGAGGCCGAACAAATCGATAGATAAATCATCGCTGTCTTTCAGCATCAg < 1:981392/69‑1 (MQ=255) cTTAATGAACACCATATGGTTGAGGCCGAACAAATCGATAGATAAATCATCGCTGTCTTTCAGCATCAg > 1:2151287/1‑69 (MQ=255) cTTAATGAACACCATATGGTTGAGGCCGAACAAATCGATAGATAAATCATCGCTGTCTTTCAGCATCAg > 1:1850601/1‑69 (MQ=255) cTTAATGAACACCATATGGTTGAGGCCGAACAAATCGATAGATAAATCATCGCTGTCTTTCAGCATCAg < 1:1090864/69‑1 (MQ=255) cTTAATGAACACCATATGGTTGAGGCCGAACAAATCGATAGATAAATCATCGCTGTCTTTCAGCATCAg > 1:2871965/1‑69 (MQ=255) cTTAATGAACACCATATGGTTGAGGCCGAACAAATCGATAGATAAATCATCGCTGTCTTTCAGCATCAg > 1:3288046/1‑69 (MQ=255) cTTAATGAACACCATATGGTTGAGGCCGAACAAATCGATAGATAAATCATCGCTGTCTTTCAGCATCAg > 1:1218547/1‑69 (MQ=255) cTTAATGAACACCATATGGTTGAGGCCGAACAAATCGATAGATAAATCATCGCTGTCTTTCAGCATCAg > 1:915947/1‑69 (MQ=255) cTTAATGAACACCATATGGTTGAGGCCGAACAAATCGATAGATAAATCATCGCTGTCTTTCAGCATCAg > 1:511740/1‑69 (MQ=255) cTTAATGAACACCATATGGTTGAGGCCGAACAAATCGATAGATAAATCATCGCTGTCTTTCAGCATCAg > 1:692998/1‑69 (MQ=255) gAACACCATATGGTTGAGGCCGAACAAATCTATAGATAAATCATCGCTGTCTTTCAGCATCAGAACAt > 1:2604486/1‑68 (MQ=255) gAACACCATATGGTTGAGGCCGAACAAATCGATAGATAAATCATCGCTGTCTTTCAGCATCAGAACATc > 1:3001162/1‑69 (MQ=255) gAACACCATATGGTTGAGGCCGAACAAATCGATAGATAAATCATCGCTGTCTTTCAGCATCAGAACATc > 1:2969998/1‑69 (MQ=255) gAACACCATATGGTTGAGGCCGAACAAATCGATAGATAAATCATCGCTGTCTTTCAGCATCAGAACATc > 1:959334/1‑69 (MQ=255) gAACACCATATGGTTGAGGCCGAACAAATCGATAGATAAATCATCGCTGTCTTTCAGCATCAGAACATc > 1:25807/1‑69 (MQ=255) gAACACCATATGGTTGAGGCCGAACAAATCGATAGATAAATCATCGCTGTCTTTCAGCATCAGAACATc > 1:2487383/1‑69 (MQ=255) gAACACCATATGGTTGAGGCCGAACAAATCGATAGATAAATCATCGCTGTCTTTCAGCATCAGAACATc > 1:2300842/1‑69 (MQ=255) gAACACCATATGGTTGAGGCCGAACAAATCGATAGATAAATCATCGCTGTCTTTCAGCATCAGAACATc > 1:226193/1‑69 (MQ=255) gAACACCATATGGTTGAGGCCGAACAAATCGATAGATAAATCATCGCTGTCTTTCAGCATCAGAACATc > 1:330201/1‑69 (MQ=255) gAACACCATATGGTTGAGGCCGAACAAATCGATAGATAAATCATCGCTGTCTTTCAGCATCAGAACATc > 1:1025238/1‑69 (MQ=255) gAACACCATATGGTTGAGGCCGAACAAATCGATAGATAAATCATCGCTGTCTTTCAGCATCAGAACATc > 1:1597387/1‑69 (MQ=255) gAACACCATATGGTTGAGGCCGAACAAATCGATAGATAAATCATCGCTGTCTTTCAGCATCAGAACATc > 1:1120925/1‑69 (MQ=255) cacCATATGGTTGAGGCCGAACAAATCGATAGATAAATCATCGCTGTCTTTCAGCATCAGAACATcgcg > 1:2193954/1‑69 (MQ=255) atatGGTTGAGGCCGAACAAATCGATAGATAAATCATCGCTGTCTTTCAGCATCAGAACATCGCGAATa < 1:1038684/69‑1 (MQ=255) atatGGTTGAGGCCGAACAAATCGATAGATAAATCATCGCTGTCTTTCAGCATCAGAACATCGCGAATa < 1:157238/69‑1 (MQ=255) atatGGTTGAGGCCGAACAAATCGATAGATAAATCATCGCTGTCTTTCAGCATCAGAACATCGCGAATa < 1:2923797/69‑1 (MQ=255) atatGGTTGAGGCCGAACAAATCGATAGATAAATCATCGCTGTCTTTCAGCATCAGAACATCGCGAATa < 1:3186662/69‑1 (MQ=255) atatGGTTGAGGCCGAACAAATCGATAGATAAATCATCGCTGTCTTTCAGCATCAGAACATCGCGAATa < 1:400858/69‑1 (MQ=255) atatGGTTGAGGCCGAACAAATCGATAGATAAATCATCGCTGTCTTTCAGCATCAGAACATCGCGAATa < 1:1366728/69‑1 (MQ=255) atatGGTTGAGGCCGAACAAATCGATAGATAAATCATCGCTGTCTTTCAGCATCAGAACATCGCGAATa < 1:2047759/69‑1 (MQ=255) atatGGTTGAGGCCGAACAAATCGATAGATAAATCATCGCAGTCTTTCAGCATCAGAACATCGCGAATa < 1:1363351/69‑1 (MQ=255) tGGTTGAGGCCGAACAAATCGATAGATAAATCATCGCTGTCTTTCAGCATCAGAACATCGCGAATa < 1:1463985/66‑1 (MQ=255) tGGTTGAGGCCGAACAAATCGATAGATAAATCATCGCTGTCTTTCAGCATCAGAACATCGCGAATa < 1:146945/66‑1 (MQ=255) tGGTTGAGGCCGAACAAATCGATAGATAAATCATCGCTGTCTTTCAGCATCAGAACATCGCGAATa < 1:866956/66‑1 (MQ=255) tGGTTGAGGCCGAACAAATCGATAGATAAATCATCGCTGTCTTTCAGCATCAGAACATCGCGAATa < 1:2704993/66‑1 (MQ=255) tGGTTGAGGCCGAACAAATCGATAGATAAATCATCGCTGTCTTTCAGCATCAGAACATCGCGAATa < 1:2059867/66‑1 (MQ=255) tGGTTGAGGCCGAACAAATCGATAGATAAATCATCGCTGTCTTTCAGCATCAGAACATCGCGAATa < 1:2575300/66‑1 (MQ=255) tGGTTGAGGCCGAACAAATCGATAGATAAATCATCGCTGTCTTTCAGCATCAGAACATCGCGAATa < 1:2546943/66‑1 (MQ=255) tGGTTGAGGCCGAACAAATCGATAGATAAATCATCGCTGTCTTTCAGCATCAGAACATCGCGAATa < 1:2072398/66‑1 (MQ=255) tGGTTGAGGCCGAACAAATCGATAGATAAATCATCGCTGTCTTTCAGCATCAGAACATCGCGAATa < 1:221344/66‑1 (MQ=255) tGGTTGAGGCCGAACAAATCGATAGATAAATCATCGCTGTCTTTCAGCATCAGAACATCGCGAATa < 1:2298552/66‑1 (MQ=255) tGGTTGAGGCCGAACAAATCGATAGATAAATCATCGCTGTCTTTCAGCATCAGAACATCGCGAATa < 1:1712008/66‑1 (MQ=255) tGGTTGAGGCCGAACAAATCGATAGATAAATCATCGCTGTCTTTCAGCATCAGAACATCGCGAATa < 1:2002931/66‑1 (MQ=255) tGGTTGAGGCCGAACAAATCGATAGATAAATCATCGCTGTCTTTCAGCATCAGAACATCGCGAATAAAc > 1:2595445/1‑69 (MQ=255) tGGTTGACGCCGAACAAATCGATAGATAAATCATCGCTGTCTTTCAGCATCAGAACATCGCGAATAAAc > 1:2410565/1‑69 (MQ=255) gTTGAGGCCGAACAAATCGATAGATAAATCATCGCTGTCTTTCAGCATCAGAACATCGCGAATAAACAt > 1:2587804/1‑69 (MQ=255) gTTGAGGCCGAACAAATCGATAGATAAATCATCGCTGTCTTTCAGCATCAGAACATCGCGAATAAACAt > 1:2274187/1‑69 (MQ=255) gTTGAGGCCGAACAAATCGATAGATAAATCATCGCTGTCTTTCAGCATCAGAACATCGCGAATAAACAt > 1:2728787/1‑69 (MQ=255) gTTGAGGCCGAACAAATCGATAGATAAATCATCGCTGTCTTTCAGCATCAGAACATCGCGAATAAACAt > 1:2731297/1‑69 (MQ=255) gTTGAGGCCGAACAAATCGATAGATAAATCATCGCTGTCTTTCAGCATCAGAACATCGCGAATAAACAt > 1:517703/1‑69 (MQ=255) gTTGAGGCCGAACAAATCGATAGATAAATCATCGCTGTCTTTCAGCATCAGAACATCGCGAATAAACAt > 1:2312305/1‑69 (MQ=255) gTTGAGGCCGAACAAATCGATAGATAAATCATCGCTGTCTTTCAGCATCAGAACATCGCGAATAAACAt > 1:1566496/1‑69 (MQ=255) gTTGAGGCCGAACAAATCGATAGATAAATCATCGCTGTCTTTCAGCATCAGAACATCGCGAATAAACAt > 1:850138/1‑69 (MQ=255) gTTGAGGCCGAACAAATCGATAGATAAATCATCGCTGTCTTTCAGCATCAGAACATCGCGAATAAACAt > 1:126214/1‑69 (MQ=255) gTTGAGGCCGAACAAATCGATAGATAAATCATCGCTGTCTTTCAGCATCAGAACATAGCGAATAAACAt > 1:1694267/1‑69 (MQ=255) gAGGCCGAACAGATCGATAGATAAATCATCGCTGTCTTTCAGCATCAGAACATCGCGAATAAACATCtt < 1:1930496/69‑1 (MQ=255) gAGGCCGAACAGATCGATAGATAAATCATCGCTGTCTTTCAGCATCAGAACATCGCGAATAAACATCtt < 1:723570/69‑1 (MQ=255) gAGGCCGAACAGATCGATAGATAAATCATCGCTGTCTTTCAGCATCAGAACATCGCGAATAAACATCtt < 1:703093/69‑1 (MQ=255) gAGGCCGAACAGATCGATAGATAAATCATCGCTGTCTTTCAGCATCAGAACATCGCGAATAAACATCtt < 1:1315332/69‑1 (MQ=255) gAGGCCGAACAGATCGATAGATAAATCATCGCTGTCTTTCAGCATCAGAACATCGCGAATAAACATCtt < 1:1345733/69‑1 (MQ=255) gAGGCCGAACAGATCGATAGATAAATCATCGCTGTCTTTCAGCATCAGAACATCGCGAATAAACATCtt < 1:557160/69‑1 (MQ=255) gAGGCCGAACAGATCGATAGATAAATCATCGCTGTCTTTCAGCATCAGAACATCGCGAATAAACATCtt < 1:972065/69‑1 (MQ=255) gAGGCCGAACAGATCGATAGATAAATCATCGCTGTCTTTCAGCATCAGAACATCGCGAATAAACATCtt < 1:1239124/69‑1 (MQ=255) gAGGCCGAACAGATCGATAGATAAATCATCGCTGTCTTTCAGCATCAGAACATCGCGAATAAACATCtt < 1:3323427/69‑1 (MQ=255) gAGGCCGAACAGATCGATAGATAAATCATCGCTGTCTTTCAGCATCAGAACATCGCGAATAAACATCtt < 1:442594/69‑1 (MQ=255) gAGGCCGAACAGATCGATAGATAAATCATCGCTGTCTTTCAGCATCAGAACATCGCGAATAAACATCtt < 1:1603815/69‑1 (MQ=255) gAGGCCGAACAGATCGATAGATAAATCATCGCTGTCTTTCAGCATCAGAACATCGCGAATAAACATCtt < 1:267303/69‑1 (MQ=255) gAGGCCGAACAGATCGATAGATAAATCATCGCTGTCTTTCAGCATCAGAACATCGCGAATAAACATCtt < 1:2506508/69‑1 (MQ=255) gAGGCCGAACAGATCGATAGATAAATCATCGCTGTCTTTCAGCATCAGAACATCGCGAATAAACATCtt < 1:14441/69‑1 (MQ=255) gAGGCCGAACAGATCGATAGATAAATCATCGCTGTCTTTCAGCATCAGAACATCGCGAATAAACATCtt < 1:1458668/69‑1 (MQ=255) gAGGCCGAACAGATCGATAGATAAATCATCGCTGTCTTTCAGCATCAGAACATCGCGAATAAACATCtt < 1:3236187/69‑1 (MQ=255) gAGGCCGAACAGATCGATAGATAAATCATCGCTGTCTTTCAGCATCAGAACATCGCGAATAAACATCtt < 1:2366423/69‑1 (MQ=255) gAGGCCGAACAGATCGATAGATAAATCATCGCTGTCTTTCAGCATCAGAACATCGCGAATAAACATCtt < 1:1761112/69‑1 (MQ=255) gAGGCCGAACAGATCGATAGATAAATCATCGCTGTCTTTCAGCATCAGAACATCGCGAATAAACATCtt < 1:1496800/69‑1 (MQ=255) gAGGCCGAACAGATCGATAGATAAATCATCGCTGTCTTTCAGCATCAGAACATCGCGAATAAACATCtt < 1:1512563/69‑1 (MQ=255) gAGGCCGAACAGATCGATAGATAAATCATCGCTGTCTTTCAACATCAGAACATCGCGAATAAACATCtt < 1:1215725/69‑1 (MQ=255) gAGGCCGAACAAATCGATAGATAAATCATCGCTGTCTTTCAGCATCAGAACATCGCGAATAAACATCtt < 1:772306/69‑1 (MQ=255) gAGGCCGAACAAATCGATAGATAAATCATCGCTGTCTTTCAGCATCAGAACATCGCGAATAAACATCtt > 1:2292366/1‑69 (MQ=255) gAGGCCGAACAAATCGATAGATAAATCATCGCTGTCTTTCAGCATCAGAACATCGCGAATAAACATCtt > 1:518977/1‑69 (MQ=255) aGGCCGAACAGATCGATAGATAAATCATCGCTGTCTTTCAGCATCAGAACATCGCGAATAAACATCtt < 1:3325237/68‑1 (MQ=255) aGGCCGAACAAATCGATAGATAAATCATCGCTGTCTTTCAGCATCAGAACATCGCGAATAAACATCtt < 1:1663070/68‑1 (MQ=255) gCCGAACAAATCGATAGATAAATCATCGCTGTCTTTCAGCATCAGAACATCGCGAATAAACATCtt < 1:1796147/66‑1 (MQ=255) | GACTTGCCATTTATCAGCACATCCTTAATGAACACCATATGGTTGAGGCCGAACAAATCGATAGATAAATCATCGCTGTCTTTCAGCATCAGAACATCGCGAATAAACATCTT > minE/1145545‑1145657 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |