| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,146,056 | G→C | 23.8% | P46A (CCG→GCG) | chbF ← | cryptic phospho‑beta‑glucosidase, NAD(P)‑binding |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,146,056 | 0 | G | C | 23.8% | 101.3 / 28.0 | 63 | P46A (CCG→GCG) | chbF | cryptic phospho‑beta‑glucosidase, NAD(P)‑binding |
| Reads supporting (aligned to +/- strand): ref base G (28/20); new base C (8/7); total (36/27) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.72e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.61e-01 | |||||||||||
AAGCTTCATCGGGACGCCAGCGTTATCAATCATCCGTTGGCAGAGATCGAAAATAATATCCAGTTTCGGTTTACCACCTTCGACATCCACCAGCCATAATTCGCTGACCGGCAATTCGTGATAACGCTTAATAA > minE/1145988‑1146121 | aaGCTTCATCGGGACGCCAGCGTTATCAATCATCCGTTGGCAGAGATCGAAAATAATATCCAGTTTCgg < 1:2755424/69‑1 (MQ=255)aaGCTTCATCGGGACGCCAGCGTTATCAATCATCCGTTGGCAGAGATCGAAAATAATATCCAGTTTCgg < 1:1382758/69‑1 (MQ=255)aaGCTTCATCGGGACGCCAGCGTTATCAATCATCCGTTGGCAGAGATCGAAAATAATATCCAGTTTCgg < 1:559046/69‑1 (MQ=255)aaGCTTCATCGGGACGCCAGCGTTATCAATCATCCGTTGGCAGAGATCGAAAATAATATCCAGTTTCgg < 1:1554428/69‑1 (MQ=255) tCATCGGGACGCCAGCGTTATCAATCATCCGTTGGCAGAGATCGAAAATAATATCCAGTTTCGGTTTac < 1:2284515/69‑1 (MQ=255) tCATCGGGACGCCAGCGTTATCAATCATCCGTTGGCAGAGATCGAAAATAATATCCAGTTTCGGTTTac < 1:3254858/69‑1 (MQ=255) cATCGGGACGCCAGCGTTATCAATCATCCGTTGGCAGAGATCGAAAATAATATCCAGTTTCGGTTTac < 1:1269294/68‑1 (MQ=255) tCGGGACGCCATCGTTATCAATCATCCGTTGGCAGAGATCGAAAATAATATCCAGTTTCGGTTTacc < 1:2534330/67‑1 (MQ=255) tCGGGACGCCAGCGTTATCAATCATCCGTTGGCAGAGATCGAAAATAATATCCAGTTTCGGTTTacc < 1:874969/67‑1 (MQ=255) tCGGGACGCCAGCGTTATCAATCATCCGTTGGCAGAGATCGAAAATAATATCCAGTTTCGGTTTacc < 1:2180966/67‑1 (MQ=255) tCGGGACGCCAGCGTTATCAATCATCCGTTGGCAGAGATCGAAAATAATATCCAGTTTCGGTTTacc < 1:2849793/67‑1 (MQ=255) tCGGGACGCCAGCGTTATCAATCATCCGTTAGCAGAGATCGAAAATAATATCCAGTTTCGGTTTacc < 1:2356756/67‑1 (MQ=255) cGGGACGCCAGCGTTATCAATCATCCGTTGGCAGAGATCGAAAATAATATCCAGTTTCGGTTTaccac < 1:1120217/68‑1 (MQ=255) cGGGACGCCAGCGTTATCAATCATCCGTTGGCAGAGATCGAAAATAATATCCAGTTTCGGTTTaccac < 1:3247617/68‑1 (MQ=255) cGGGACGCCAGCGTTATCAATCATCCGTTGGCAGAGATCGAAAATAATATCCAGTTTCGGTTTaccac < 1:3435157/68‑1 (MQ=255) aCGCCAGCGTTATCAATCATCCGTTGGCAGAGATCGAAAATAATATCCAGTTTCgg < 1:2015365/56‑1 (MQ=255) atcaatcaTCCGTTGGCAGAGATCAAAAATAATATCCAGTTTCGCtt > 1:1343573/1‑47 (MQ=39) atcaatcaTCCGTTGGCAGAGATCAAAAATAATATCCAGTTTCGCtt > 1:2796999/1‑47 (MQ=39) atcaatcaTCCGTTGGCAGAGATCAAAAATAATATCCAGTTTCGCtt > 1:1339803/1‑47 (MQ=39) atcaatcaTCCGTTGGCAGAGATCAAAAATAATATCCAGTTTCGCtt > 1:2539531/1‑47 (MQ=39) atcaatcaTCCGTTGGCAGAGATCAAAAATAATATCCAGTTTCGCtt > 1:1271958/1‑47 (MQ=39) atcaatcaTCCGTTGGCAGAGATCAAAAATAATATCCAGTTTCGCtt > 1:2138626/1‑47 (MQ=39) atcaatcaTCCGTTGGCAGAGATCAAAAATAATATCCAGTTTCGCtt > 1:1178160/1‑47 (MQ=39) atcaatcaTCCGTTGGCAGAGATCAAAAATAATATCCAGTTTCGCtt > 1:2258682/1‑47 (MQ=39) atcCGTTGGCAGAGATCGAAAATAATATCCAGTTTCGGt > 1:2301189/1‑39 (MQ=255) atcCGTTGGCAGAGATCGAAAATAATATCCAGTTTCGGTTTACCACCTTCGACATcc > 1:3101218/1‑57 (MQ=255) atcCGTTGGCAGAGATCGAAAATAATATCCAGTTTCGGTTTACCACCTTCGACATcc > 1:2379098/1‑57 (MQ=255) atcCGTTGGCAGAGATCGAAAATAATATCCAGTTTCGGTTTACCACCTTCGACATcc > 1:691004/1‑57 (MQ=255) atcCGTTGGCAGAGATCGAAAATAATATCCAGTTTCGGTTTACCACCTTCGACATcc > 1:2519206/1‑57 (MQ=255) atcCGTTGGCAGAGATCGAAAATAATATCCAGTTTCGGTTTACCACCTTCGACATcc > 1:1779274/1‑57 (MQ=255) atcCGTTGGCAGAGATCGAAAATAATATCCAGTTTCGGTTTACCACCTTCGACATcc > 1:1918672/1‑57 (MQ=255) atcCGTTGGCAGAGATCGAAAATAATATCCAGTTTCGGTTTACCACCTTCGACATcc > 1:1414541/1‑57 (MQ=255) atcCGTTGGCAGAGATCGAAAATAATATCCAGTTTCGGTTTACCACCTTCGACATc > 1:1935402/1‑56 (MQ=255) gaTCGAAAATAATATCCAGTTTCGGTTTACCACCTTCGACATCCACCAGCCATAATTCGCTGACCGGCa < 1:1210714/69‑1 (MQ=255) gaTCGAAAATAATATCCAGTTTCGGTTTACCACCTTCGACATCCACCAGCCATAATTCGCTGACCGGCa < 1:2287865/69‑1 (MQ=255) gaTCGAAAATAATATCCAGTTTCGGTTTACCACCTTCGACATCCACCAGCCATAATTCGCTGACCGGCa < 1:2968027/69‑1 (MQ=255) gaTCGAAAATAATATCCAGTTTCGGTTTACCACCTTCGACATCCACCAGCCATAATTCGCTGACCGGCa < 1:2949591/69‑1 (MQ=255) gaTCGAAAATAATATCCAGTTTCGGTTTACCACCTTCGACATCCACCAGCCATAATTCGCTGACCGGCa < 1:367670/69‑1 (MQ=255) gaTCGAAAATAATATCCAGTTTCGGTTTACCACCTTCGACATCCACCAGCCATAATTCGCTGACCGGCa < 1:1132533/69‑1 (MQ=255) gaTCGAAAATAATATCCAGTTTCGGTTTACCACCTTCGACATCCACCAGCCATAATTCGCTGACCGGCa < 1:2398913/69‑1 (MQ=255) gaTCGAAAATAATATCCAGTTTCGGTTTACCACCTTCGACATCCACCAGCCATAATTCGCTGACCGGCa < 1:994138/69‑1 (MQ=255) aataTCCAGTTTCGGTTTACCACCTTCGACATCCACCAGCCATAATTCGCTGACCGGCAATTCGTGAt > 1:2501181/1‑68 (MQ=255) aataTCCAGTTTCGGTTTACCACCTTCGACATCCACCAGCCATAATTCGCTGACCGGCAATTCGTGATa > 1:733005/1‑69 (MQ=255) aataTCCAGTTTCGGTTTACCACCTTCGACATCCACCAGCCATAATTCGCTGACCGGCAATTCGTGATa > 1:1752485/1‑69 (MQ=255) aataTCCAGTTTCGGTTTACCACCTTCGACATCCACCAGCCATAATTCGCTGACCGGCAATTCGTGATa > 1:399642/1‑69 (MQ=255) aataTCCAGTTTCGGTTTACCACCTTCGACATCCACCAGCCATAATTCGCTGACCGGCAATTCGTGATa > 1:910758/1‑69 (MQ=255) aataTCCAGTTTCGGTTTACCACCTTCGACATCCACCAGCCATAATTCGCTGACCGGCAATTCGTGATa > 1:976854/1‑69 (MQ=255) aataTCCAGTTTCGGTTTACCACCTTCGACATCCACCAGCCATAATTCGCTGACCGGCAATTCGTGATa > 1:989515/1‑69 (MQ=255) aataTCCAGTTTCGGTTTACCACCTTCGACATCCACCAGCCATAATTCGCTGACCGGCAATTCGTGATa > 1:99993/1‑69 (MQ=255) aataTCCAGTTTCGGTTTACCACCTTCGACATCCACCAGCCATAATTCGCTGACCGGCAATTCGTGATa > 1:2048190/1‑69 (MQ=255) aataTCCAGTTTCGGTTTACCACCTTCGACATCCACCAGCCATAATTCGCTGACCGGCAATTCGTGATa > 1:1863516/1‑69 (MQ=255) aataTCCAGTTTCGGTTTACCACCTTCGACATCCACCAGCCATAATTCGCTGACCGGCAATTCGTGATa > 1:2656666/1‑69 (MQ=255) atatCCAGTTTCGGTTTACCACCTTCGACATCCACCAGCCATAATTCGCTGACCGGCa < 1:2653804/58‑1 (MQ=255) atatCCAGTTTCGCTTTACCACCTTCGACATCCACCAGCCATAATTCGCTGACCGGCAATTCGTGATaa < 1:2243945/69‑1 (MQ=255) atatCCAGTTTCGCTTTACCACCTTCGACATCCACCAGCCATAATTCGCTGACCGGCAATTCGTGATaa < 1:99240/69‑1 (MQ=255) atatCCAGTTTCGCTTTACCACCTTCGACATCCACCAGCCATAATTCGCTGACCGGCAATTCGTGATaa < 1:2046492/69‑1 (MQ=255) atatCCAGTTTCGCTTTACCACCTTCGACATCCACCAGCCATAATTCGCTGACCGGCAATTCGTGATaa < 1:880895/69‑1 (MQ=255) atatCCAGTTTCGCTTTACCACCTTCGACATCCACCAGCCATAATTCGCTGACCGGCAATTCGTGATaa < 1:40110/69‑1 (MQ=255) atatCCAGTTTCGCTTTACCACCTTCGACATCCACCAGCCATAATTCGCTGACCGGCAATTCGTGATaa < 1:2740365/69‑1 (MQ=255) atatCCAGTTTCGCTTTACCACCTTCGACATCCACCAGCCATAATTCGCTGACCGGCAATTCGTGATaa < 1:364980/69‑1 (MQ=255) ttCGGTTTACCACCTTCGACATCCACCAGCCATAATTCGCTGACCGGCAATTCGTGATAACGCTtaata > 1:1993855/1‑69 (MQ=255) tCGGTTTACCACCTTCGACATCCACCAGCCATAATTCGCTGACCGGCAATTCGTGATAACGCTtaataa > 1:2739879/1‑69 (MQ=255) tCGGTTTACCACCTTCGACATCCACCAGCCATAATTCGCTGACCGGCAATTCGTGATAACGCTtaataa > 1:625462/1‑69 (MQ=255) tCGGTTTACCACCTTCGACATCCACCAGCCATAATTCGCTGACCGGCAATTCGTGATAACGCTtaataa > 1:2610052/1‑69 (MQ=255) tCGGTTTACCACCTTCGACATCCACCAGCCATAATTCGCTGACCGGCAATTCGTGATAACGCTtaataa > 1:369298/1‑69 (MQ=255) tCGGTTTACCACCTTCGACATCCACCAGCCATAATTCGCTGACCGGCAATTCGTGATAACGCTtaataa > 1:1114736/1‑69 (MQ=255) tCGGTTTACCACCTTCGACATCCACCAGCCATAATTCGCTGACCGGCAATTCGTGATAACGCTtaataa > 1:1589724/1‑69 (MQ=255) tCGGTTTACCACCTTCGACATCCACCAGCCATAATTCGCTGACCGGCAATTCGTGATAACGCTtaataa > 1:3306848/1‑69 (MQ=255) gTTTACCACCTTCGACATCCACCAGCCATAATTCGCTGACCGGCa < 1:1816261/45‑1 (MQ=255) | AAGCTTCATCGGGACGCCAGCGTTATCAATCATCCGTTGGCAGAGATCGAAAATAATATCCAGTTTCGGTTTACCACCTTCGACATCCACCAGCCATAATTCGCTGACCGGCAATTCGTGATAACGCTTAATAA > minE/1145988‑1146121 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |