| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,149,753 | G→A | 14.4% | R65R (CGC→CGT) | osmE ← | DNA‑binding transcriptional regulator |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,149,753 | 0 | G | A | 14.4% | 184.5 / 14.6 | 103 | R65R (CGC→CGT) | osmE | DNA‑binding transcriptional regulator |
| Reads supporting (aligned to +/- strand): ref base G (38/50); new base A (7/8); total (45/58) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.50e-01 | |||||||||||
Only 100 of 103 total aligned reads displayed.
GCGACAAAGTAGGTTTCTGCTTTACCATCACGTTGACCCAGGATGTAGGTCTGGCAAGTACCGCGAGCATGGATCATGCTCACTTCAGACGAAGGTTTACCCGCAATTTGTGCAACCTGCGCCCGGCTC > minE/1149691‑1149819 | gcgACAAAGTAGGTTTCTGCTTTACCATCACGTTGACCCAGGATGTAGGTCTGGCAAGTACCGCGAg < 1:1730705/67‑1 (MQ=255)gcgACAAAGTAGGTTTCTGCTTTACCATCACGTTGACCCAGGATGTAGGTCTGGCAAGTACCGCGAg < 1:509326/67‑1 (MQ=255) aaaGTAGGTTTCTGCTTTACCATCACGTTGACCTAGGATGTAGGTCTGGCAAGTACCGCGAGCATGGAt < 1:2661901/69‑1 (MQ=255) aaaGTAGGTTTCTGCTTTACCATCACGTTGACCCAGGATGTAGGTCTGGCAAGTACCGCGAGCATGGAt < 1:1511477/69‑1 (MQ=255) aaaGTAGGTTTCTGCTTTACCATCACGTTGACCCAGGATGTAGGTCTGGCAAGTACCGCGAGCATGGAt > 1:1857503/1‑69 (MQ=255) aaaGTAGGTTTCTGCTTTACCATCACGTTGACCCAGGATGTAGGTCTGGCAAGTACCGCGAGCATGGAt < 1:2015741/69‑1 (MQ=255) aaaGTAGGTTTCTGCTTTACCATCACGTTGACCCAGGATGTAGGTCTGGCAAGTACCGCGAGCATGGAt < 1:176537/69‑1 (MQ=255) aaaGTAGGTTTCTGCTTTACCATCACGTTGACCCAGGATGTAGGTCTGGCAAGTACCGCGAGCATGGAt > 1:2353193/1‑69 (MQ=255) aaaGTAGGTTTCTGCTTTACCATCACGTTGACCCAGGATGTAGGTCTGGCAAGTACCGCGAGCATGGAt < 1:1736597/69‑1 (MQ=255) aaaGTAGGTTTCTGCTTTACCATCACGTTGACCCAGGATGTAGGTCTGGCAAGTACCGCGAGCATGGAt < 1:2528846/69‑1 (MQ=255) aaaGTAGGTTTCTGCTTTACCATCACGTTGACCCAGGATGTAGGTCTGGCAAGTACCGCGAGCATGGAt < 1:1666642/69‑1 (MQ=255) aaaGTAGGTTTCTGCTTTACCATCACGTTGACCCAGGATGTAGGTCTGGCAAGTACCGCGAGCATGGAt < 1:1649644/69‑1 (MQ=255) aaaGTAGGTTTCTGCTTTACCATCACGTTGACCCAGGATGTAGGTCTGGCAAGTACCGCGAGCATGGAt < 1:266988/69‑1 (MQ=255) aaaGTAGGTTTCTGCTTTACCATCACGTTGACCCAGGATGTAGGTCTGGCAAGTACCGCGAGCATGGAt < 1:2743712/69‑1 (MQ=255) aaaGTAGGTTTCTGCTTTACCATCACGTTGACCCAGGATGTAGGTCTGGCAAGTACCGCGAGCATGGAt < 1:3180432/69‑1 (MQ=255) aaaGTAGGTTTCTGCTTTACCATCACGTTGACCCAGGATGTAGGTCTGGCAAGTACCGCGAGCATGGAt < 1:318977/69‑1 (MQ=255) aaaGTAGGTTTCTGCTTTACCATCACGTTGACCCAGGATGTAGGTCTGGCAAGTACCGCGAGCATGGAt > 1:3191118/1‑69 (MQ=255) aaaGTAGGTTTCTGCTTTACCATCACGTTGACCCAGGATGTAGGTCTGGCAAGTACCGCGAGCATGGAt < 1:3234463/69‑1 (MQ=255) aaaGTAGGTTTCTGCTTTACCATCACGTTGACCCAGGATGTAGGTCTGGCAAGTACCGCGAGCATGGAt < 1:1323194/69‑1 (MQ=255) aaaGTAGGTTTCTGCTTTACCATCACGTTGACCCAGGATGTAGGTCTGGCAAGTACCGCGAGCATGGAt < 1:3349047/69‑1 (MQ=255) aaaGTAGGTTTCTGCTTTACCATCACGTTGACCCAGGATGTAGGTCTGGCAAGTACCGCGAGCATGGAt < 1:410840/69‑1 (MQ=255) aaaGTAGGTTTCTGCTTTACCATCACGTTGACCCAGGATGTAGGTCTGGCAAGTACCGCGAGCATGGAt < 1:567343/69‑1 (MQ=255) aaaGTAGGTTTCTGCTTTACCATCACGTTGACCCAGGATGTAGGTCTGGCAAGTACCGCGAGCATGGAt < 1:891107/69‑1 (MQ=255) aaaGTAGGTTTCTGCTTTACCATCACGTTGACCCAGGATGTAGGTCTGGCAAGTACCGCGAGCATGGAt < 1:1086367/69‑1 (MQ=255) aaaGTAGGTTTCTGCTTTACCATCACGTTGACCCAGGATGTAGGTCTGGCAAGTACCGCGAGCATGGAt < 1:913783/69‑1 (MQ=255) aaaGTAGGTTTCTGCTTTACCATCACGTTGACCCAGGATGTAGGTCTGGCAAGTACCGCGAGCATGGAt < 1:105214/69‑1 (MQ=255) aaaGTAGGTTTCTGCTTTACCATCACGTTGACCCAGGATGTAGGTCTGGCAAGTACCGCGAGCATGGAt > 1:1060443/1‑69 (MQ=255) aaGTATGTTTCTGCTTTACCATCACGTTGACCCAGGATGTAGGTCTGGCAAGTACCGCGAGCATGGAt < 1:947624/68‑1 (MQ=255) aaGTAGGTTTCTGCTTTACCATCACGTTGACCCAGGATGTAGGTCTGGCAAGTACCGCGAGCATGGAt < 1:1764269/68‑1 (MQ=255) aaGTAGGTTTCTGCTTTACCATCACGTTGACCCAGGATGTAGGTCTGGCAAGTACCGCGAGCATGGAt < 1:2300665/68‑1 (MQ=255) tAGGTTTCTGCTTTACCATCACGTTGACCCAGGATGTAGGTCTGGCAAGTACCGCGAGCATGTATCATg < 1:2939/69‑1 (MQ=255) tAGGTTTCTGCTTTACCATCACGTTGACCCAGGATGTAGGTCTGGCAAGTACCGCGAGCATGGATCATg < 1:1825702/69‑1 (MQ=255) tAGGTTTCTGCTTTACCATCACGTTGACCCAGGATGTAGGTCTGGCAAGTACCGCGAGCATGGATCATg < 1:171845/69‑1 (MQ=255) tAGGTTTCTGCTTTACCATCACGTTGACCCAGGATGTAGGTCTGGCAAGTACCGCGAGCATGGATCATg < 1:796131/69‑1 (MQ=255) tAGGTTTCTGCTTTACCATCACGTTGACCCAGGATGTAGGTCTGGCAAGTACCGCGAGCATGGATCATg < 1:2564946/69‑1 (MQ=255) tAGGTTTCTGCTTTACCATCACGTTGACCCAGGATGTAGGTCTGGCAAGTACCGCGAGCATGGATCATg < 1:925703/69‑1 (MQ=255) tAGGTTTCTGCTTTACCATCACGTTGACCCAGGATGTAGGTCTGGCAAGTACCGCGAGCATGGATCATg < 1:2181851/69‑1 (MQ=255) tAGGTTTCTGCTTTACCATCACGTTGACCCAGGATGTAGGTCTGGCAAGTACCGCGAGCATGGATCATg < 1:2177983/69‑1 (MQ=255) tAGGTTTCTGCTTTACCATCACGTTGACCCAGGATGTAGGTCTGGCAAGTACCGCGAGCATGGATCATg < 1:2118014/69‑1 (MQ=255) tAGGTTTCTGCTTTACCATCACGTTGACCCAGGATGTAGGTCTGGCAAGTACCGCGAGCATGGATCATg < 1:2116019/69‑1 (MQ=255) tAGGTTTCTGCTTTACCATCACGTTGACCCAGGATGTAGGTCTGGCAAGTACCGCGAGCATGGATCATg < 1:600829/69‑1 (MQ=255) tAGGTTTCTGCTTTACCATCACGTTGACCCAGGATGTAGGTCTGGCAAGTACCGCGAGCATGGATCATg < 1:1430888/69‑1 (MQ=255) tAGGTTTCTGCTTTACCATCACGCTGACCCAGGATGTAGGTCTGGCAAGTACCGCGAGCATGGATCATg < 1:1130141/69‑1 (MQ=255) gTTTCTGCTTTACCATCACGTTGACCCAGGATGTAGGTCTGGCAAGTACCGCGAGCATGGATCATGCt > 1:286615/1‑68 (MQ=255) gTTTCTGCTTTACCATCACGTTGACCCAGGATGTAGGTCTGGCAAGTACCGCGAGCATGGATCATGCTc > 1:30457/1‑69 (MQ=255) gTTTCTGCTTTACCATCACGTTGACCCAGGATGTAGGTCTGGCAAGTACCGCGAGCATGGATCATGCTc > 1:458166/1‑69 (MQ=255) gTTTCTGCTTTACCATCACGTTGACCCAGGATGTAGGTCTGGCAAGTACCGCGAGCATGGATCATGCTc > 1:1495814/1‑69 (MQ=255) gTTTCTGCTTTACCATCACGTTGACCCAGGATGTAGGTCTGGCAAGTACCGCGAGCATGGATCATGCTc > 1:2949051/1‑69 (MQ=255) gTTTCTGCTTTACCATCACGTTGACCCAGGATGTAGGTCTGGCAAGTACCGCGAGCATGGATCATGCTc > 1:628251/1‑69 (MQ=255) gTTTCTGCTTTACCATCACGTTGACCCAGGATGTAGGTCTGGCAAGTACCGCGAGCATGGATCATGCTc > 1:1396014/1‑69 (MQ=255) gTTTCTGCTTTACCATCACGTTGACCCAGGATGTAGGTCTGGCAAGTACCGCGAGCATGGATCATGCTc > 1:632541/1‑69 (MQ=255) gTTTCTGCTTTACCATCACGTTGACCCAGGATGTAGGTCTGGCAAGTACCGCGAGCATGGATCATGCTc > 1:156871/1‑69 (MQ=255) gTTTCTGCTTTACCATCACGTTGACCCAGGATGTAGGTCTGGCAAGTACCGCGAGCATGGATCATGCTc > 1:33513/1‑69 (MQ=255) gTTTCTGCTTTACCATCACGTTGACCCAGGATGTAGGTCTGGCAAGTACCGCGAGCATGGATCATGCTc > 1:389287/1‑69 (MQ=255) gTTTCTGCTTTACCATCACGTTGACCCAGGATGTAGGTCTGGCAAGTACCGCGAGCATGGATCATGCTc > 1:855454/1‑69 (MQ=255) gTTTCTGCTTTACCATCACGTTGACCCAGGATGTAGGTCTGGCAAGTACCGCGAGCATGGATCATGCTc > 1:243387/1‑69 (MQ=255) gTTTCTGCTTTACCATCACGTTGACCCAGGATGTAGGTCTGGCAAGTACCGCGAGCATGGATCATGCTc > 1:2139060/1‑69 (MQ=255) tttCTGCTTTACCATCACGTTGACCCAGGATGTAGGTCTGGCAAGTACCGCGAGCATGGATCATGCTc < 1:3258862/68‑1 (MQ=255) tttCTGCTTTACCATCACGTTGACCCAGGATGTAGGTCTGGCAAGTACCGCGAGCATGGATCATGCTc < 1:2687749/68‑1 (MQ=255) tttCTGCTTTACCATCACGTTGACCCAGGATGTAGGTCTGGCAAGTACCGCGAGCATGGATCATGCTc < 1:461450/68‑1 (MQ=255) tttCTGCTTTACCATCACGTTGACCCAGGATGTAGGTCTGGCAAGTACCGCGAGCATGGATCATGCTc < 1:2907084/68‑1 (MQ=255) tttCTGCTTTACCATCACGTTGACCCAGGATGTAGGTCTGGCAAGTACCGCGAGCATGGATCATGCTc < 1:1799907/68‑1 (MQ=255) tCTGCTTTACCATCACGTTGACCCAGGATGTAGGTCTGGCAAGTACCGCGAGCATGGAt < 1:908578/59‑1 (MQ=255) ttACCATCACGTTGACCCAGGATGTAGGTCTGGCAAGTACCACGAGCATTGATCATGCTCACTTCa < 1:2055772/66‑1 (MQ=255) ttACCATCACGTTGACCCAGGATGTAGGTCTGGCAAGTACCACGAGCATGGATCATGCTCACTTCa < 1:1083968/66‑1 (MQ=255) ttACCATCACGTTGACCCAGGATGTAGGTCTGGCAAGTACCACGAGCATGGATCATGCTCACTTCa < 1:1277829/66‑1 (MQ=255) ttACCATCACGTTGACCCAGGATGTAGGTCTGGCAAGTACCACGAGCATGGATCATGCTCACTTCa < 1:928589/66‑1 (MQ=255) ttACCATCACGTTGACCCAGGATGTAGGTCTGGCAAGTACCACGAGCATGGATCATGCTCACTTCa < 1:1161168/66‑1 (MQ=255) ttACCATCACGTTGACCCAGGATGTAGGTCTGGCAAGTACCACGAGCATGGATCATGCTCACTTCa < 1:3015534/66‑1 (MQ=255) ttACCATCACGTTGACCCAGGATGTAGGTCTGGCAAGTACCACGAGCATGGATCATGCTCACTTCa < 1:2499844/66‑1 (MQ=255) ttACCATCACGTTGACCCAGGATGTAGGTCTGGCAAGTACCACGAGCATGGATCATGCTCACTTCa < 1:2634097/66‑1 (MQ=255) aCGTTGACCCAGGATGTAGGTCTGGCAAGTACCGCGAGCATGGATCATGCTCACTTCAGACGAAGGttt > 1:934289/1‑69 (MQ=255) aCGTTGACCCAGGATGTAGGTCTGGCAAGTACCGCGAGCATGGATCATGCTCACTTCAGACGAAGGttt > 1:727709/1‑69 (MQ=255) aCGTTGACCCAGGATGTAGGTCTGGCAAGTACCGCGAGCATGGATCATGCTCACTTCAGACGAAGGttt > 1:1073960/1‑69 (MQ=255) aCGTTGACCCAGGATGTAGGTCTGGCAAGTACCGCGAGCATGGATCATGCTCACTTCAGACGAAGGttt > 1:1971554/1‑69 (MQ=255) aCGTTGACCCAGGATGTAGGTCTGGCAAGTACCGCGAGCATGGATCATGCTCACTTCAGACGAAGGttt > 1:1135833/1‑69 (MQ=255) aGGATGTAGGTCTGGCAAGTACCACGAGCATGGATCATGCTCACTTCAGACGAAGGTTTAc > 1:2459074/1‑61 (MQ=255) aGGATGTAGGTCTGGCAAGTACCACGAGCATGGATCATGCTCACTTCAGACGAAGGTTTACCCGCAAtt > 1:847732/1‑69 (MQ=255) aGGATGTAGGTCTGGCAAGTACCACGAGCATGGATCATGCTCACTTCAGACGAAGGTTTACCCGCAAtt > 1:2992434/1‑69 (MQ=255) aGGATGTAGGTCTGGCAAGTACCACGAGCATGGATCATGCTCACTTCAGACGAAGGTTTACCCGCAAtt > 1:2219309/1‑69 (MQ=255) aGGATGTAGGTCTGGCAAGTACCACGAGCATGGATCATGCTCACTTCAGACGAAGGTTTACCCGCAAtt > 1:2247297/1‑69 (MQ=255) aGGATGTAGGTCTGGCAAGTACCACGAGCATGGATCATGCTCACTTCAGACGAAGGTTTACCCGCAAtt > 1:3076812/1‑69 (MQ=255) aGGATGTAGGTCTGGCAAGTACCACGAGCATGGATCATGCTCACTTCAGACGAAGGTTTACCCGCAAtt > 1:3068318/1‑69 (MQ=255) tGTAGGTCTGGCAAGTACCGCGAGCATGGATCATGCTCACTTCAGACGAAGGTTTACCCGCAATTtgt > 1:45764/1‑68 (MQ=255) tGTAGGTCTGGCAAGTACCGCGAGCATGGATCATGCTCACTTCAGACGAAGGTTTACCCGCAATTtgt > 1:2663070/1‑68 (MQ=255) ggTCTGGCAAGTACCGCGAGCATGGATCATGCTCACTTCAGACGAAGGTTTACCCGCAATTTGTGCAAc > 1:1875135/1‑69 (MQ=255) ggTCTGGCAAGTACCGCGAGCATGGATCATGCTCACTTCAGACGAAGGTTTACCCGCAATTTGTGCAAc > 1:2473598/1‑69 (MQ=255) ggTCTGGCAAGTACCGCGAGCATGGATCATGCTCACTTCAGACGAAGGTTTACCCGCAATTTGTGCAAc > 1:901356/1‑69 (MQ=255) ggTCTGGCAAGTACCGCGAGCATGGATCATGCTCACTTCAGACGAAGGTTTACCCGCAATTTGTGCAAc > 1:2614219/1‑69 (MQ=255) ggTCTGGCAAGTACCGCGAGCATGGATCATGCTCACTTCAGACGAAGGTTTACCCGCAATTTGTGCAAc > 1:1635499/1‑69 (MQ=255) ggTCTGGCAAGTACCGCGAGCATGGATCATGCTCACTTCAGACGAAGGTTTACCCGCAATTTGTGCAAc > 1:708623/1‑69 (MQ=255) ggTCTGGCAAGTACCGCGAGCATGGATCATGCTCACTTCAGACGAAGGTTTACCCGCAATTTGTGCAAc > 1:2793089/1‑69 (MQ=255) ggTCTGGCAAGTACCGCGAGCATGGATCATGCTCACTTCAGACGAAGGTTTACCCGCAATTTGTGCAAc > 1:49935/1‑69 (MQ=255) ggTCTGGCAAGTACCGCGAGCATGGATCATGCTCACTTCAGACGAAGGTTTACCCGCAATTTGTGCAAc > 1:1263458/1‑69 (MQ=255) cTGGCAAGTACCGCGAGCATGGATCATGCTCACTTCAGACGAAGGTTTACCCGCAATTTGTGCAACCTg > 1:1904606/1‑69 (MQ=255) aaGTACCGCGAGCATGGATCATGCTCACTTCTGACGAAGGTTTACCCGCAATTTGTGCAACCTgcg < 1:1995033/66‑1 (MQ=255) aaGTACCGCGAGCATGGATCATGCTCACTTCAGACGAAGGTTTACCCGCAATTTGTGCAACCTgcg < 1:2948060/66‑1 (MQ=255) aaGTACCGCGAGCATGGATCATGCTCACTTCAGACGAAGGTTTACCCGCAATTTGTGCAACCTgcg < 1:1528244/66‑1 (MQ=255) tACCGCGAGCATGGATCATGCTCACTTCAGACGAAGGTTTACCCGCAATTTGTGCAAcc > 1:1720399/1‑59 (MQ=255) ccGCGAGCATGGATCATGCTCACTTCAGACGAAGGTTTACCCGCAATTTGTGCAACCTGCGCCCGGCTc > 1:2626442/1‑69 (MQ=255) | GCGACAAAGTAGGTTTCTGCTTTACCATCACGTTGACCCAGGATGTAGGTCTGGCAAGTACCGCGAGCATGGATCATGCTCACTTCAGACGAAGGTTTACCCGCAATTTGTGCAACCTGCGCCCGGCTC > minE/1149691‑1149819 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |