| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,153,806 | C→T | 70.1% | S270N (AGT→AAT) | astE ← | succinylglutamate desuccinylase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,153,806 | 0 | C | T | 70.1% | 42.8 / 20.9 | 50 | S270N (AGT→AAT) | astE | succinylglutamate desuccinylase |
| Reads supporting (aligned to +/- strand): ref base C (2/13); new base T (27/8); total (29/21) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.27e-05 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GTTCCTCTCCGTCCTGCGCCAGCAATGTTCCTTTCTCAAACGGCATAAAATTCAGCGTGTCACTTGCCATATGCATTTCGAAGGACGGCGAGTGGCGAGTAATTTGCGAAACCACCCGAT > minE/1153744‑1153863 | gTTCCTCTCCGTCCTGCGCCAGCAATGTTCCTTTCTCAAACGGCATAAAATTCAGCGTGTCACTTGCCa < 1:644422/69‑1 (MQ=255)gTTCCTCTCCGTCCTGCGCCAGCAATGTTCCTTTCTCAAACGGCATAAAATTCAGCGTGTCACTTGCCa < 1:2591555/69‑1 (MQ=255)gTTCCTCTCCGTCCTGCGCCAGCAATGTTCCTTTCTCAAACGGCATAAAATTCAGCGTGTCACTTGCCa < 1:1431788/69‑1 (MQ=255)gTTCCTCTCCGTCCTGCGCCAGCAATGTTCCTTTCTCAAACGGCATAAAATTCAGCGTGTCACTTGCCa < 1:3251575/69‑1 (MQ=255)gTTCCTCTCCGTCCTGCGCCAGCAATGTTCCTTTCTCAAACGACATAAAATTCAGCGTGTCACTTGCCa < 1:2635380/69‑1 (MQ=255) gcgcCAGCAATGTTCCTTTCTCAAACGGCATAAAATTCAGCGTGTCACTTGCCATATGCATTTCGAAg < 1:43965/68‑1 (MQ=255) ccTTTCTCAAACGGCATAAAATTCAGCGTGTCACTTGCCATATGCATTTCGAAGGACggcgagtggcg > 1:1293532/1‑68 (MQ=255) ctcAAACGGCATAAAATTCAGCGTGTCTTTTGCCATATGCATTTc > 1:1370526/1‑45 (MQ=38) ctcAAACGGCATAAAATTCAGCGTGTCATTTGCCat > 1:3296033/1‑36 (MQ=255) ctcAAACGGCATAAAATTCAGCGTGTCATTTGCCATATGCATTTCGAAGGACggcga > 1:2269079/1‑57 (MQ=255) ctcAAACGGCATAAAATTCAGCGTGTCATTTGCCATATGCATTTCGAAGGACggcga > 1:1009051/1‑57 (MQ=255) ctcAAACGGCATAAAATTCAGCGTGTCATTTGCCATATGCATTTCGAAGGACggcga > 1:2449735/1‑57 (MQ=255) ctcAAACGGCATAAAATTCAGCGTGTCATTTGCCATATGCATTTCGAAGGACggcga > 1:2624268/1‑57 (MQ=255) ctcAAACGGCATAAAATTCAGCGTGTCATTTGCCATATGCATTTCGAAGGACggcga > 1:2832692/1‑57 (MQ=255) ctcAAACGGCATAAAATTCAGCGTGTCATTTGCCATATGCATTTCGAAGGACggcga > 1:2874219/1‑57 (MQ=255) ctcAAACGGCATAAAATTCAGCGTGTCATTTGCCATATGCATTTCGAAGGACggcga > 1:2943769/1‑57 (MQ=255) ctcAAACGGCATAAAATTCAGCGTGTCATTTGCCATATGCATTTCGAAGGACggcga > 1:325557/1‑57 (MQ=255) ctcAAACGGCATAAAATTCAGCGTGTCATTTGCCATATGCATTTCGAAGGACggcga > 1:3351662/1‑57 (MQ=255) ctcAAACGGCATAAAATTCAGCGTGTCATTTGCCATATGCATTTCGAAGGACggcga > 1:3378165/1‑57 (MQ=255) ctcAAACGGCATAAAATTCAGCGTGTCATTTGCCATATGCATTTCGAAGGACggcga > 1:483061/1‑57 (MQ=255) ctcAAACGGCATAAAATTCAGCGTGTCATTTGCCATATGCATTTCGAAGGACggcga > 1:604009/1‑57 (MQ=255) ctcAAACGGCATAAAATTCAGCGTGTCATTTGCCATATGCATTTCGAAGGACggcga > 1:892976/1‑57 (MQ=255) ctcAAACGGCATAAAATTCAGCGTGTCATTTGCCATATGCATTTCGAAGGACggcga > 1:225950/1‑57 (MQ=255) ctcAAACGGCATAAAATTCAGCGTGTCATTTGCCATATGCATTTCGAAGGACggcga > 1:2258312/1‑57 (MQ=255) ctcAAACGGCATAAAATTCAGCGTGTCATTTGCCATATGCATTTCGAAGGACggcga > 1:2249430/1‑57 (MQ=255) ctcAAACGGCATAAAATTCAGCGTGTCATTTGCCATATGCATTTCGAAGGACggcga > 1:2113416/1‑57 (MQ=255) ctcAAACGGCATAAAATTCAGCGTGTCATTTGCCATATGCATTTCGAAGGACggcga > 1:1898986/1‑57 (MQ=255) ctcAAACGGCATAAAATTCAGCGTGTCATTTGCCATATGCATTTCGAAGGACggcga > 1:1000109/1‑57 (MQ=255) ctcAAACGGCATAAAATTCAGCGTGTCATTTGCCATATGCATTTCGAAGGACggcga > 1:1877620/1‑57 (MQ=255) ctcAAACGGCATAAAATTCAGCGTGTCATTTGCCATATGCATTTCGAAGGACggcga > 1:1817186/1‑57 (MQ=255) ctcAAACGGCATAAAATTCAGCGTGTCATTTGCCATATGCATTTCGAAGGACggcga > 1:1714872/1‑57 (MQ=255) ctcAAACGGCATAAAATTCAGCGTGTCATTTGCCATATGCATTTCGAAGGACggcga > 1:1364187/1‑57 (MQ=255) ctcAAACGGCATAAAATTCAGCGTGTCATTTGCCATATGCATTTCGAAGGACggcga > 1:137917/1‑57 (MQ=255) ctcAAACGGCATAAAATTCAGCGTGTCATTTGCCATATGCATTTCGAAGGACggcg > 1:1491413/1‑56 (MQ=255) cATAAAATTCAGCGTGTCATTTGCCATATGCATTTCGAAGGACGGCTAGTGGCGAGTAATTTGCGAAac < 1:143110/69‑1 (MQ=255) cATAAAATTCAGCGTGTCATTTGCCATATGCATTTCGAAGGACGGCGAGTGGCGAGTAATTTGCGAAac < 1:941126/69‑1 (MQ=255) cATAAAATTCAGCGTGTCATTTGCCATATGCATTTCGAAGGACGGCGAGTGGCGAGTAATTTGCGAAac < 1:80140/69‑1 (MQ=255) cATAAAATTCAGCGTGTCATTTGCCATATGCATTTCGAAGGACGGCGAGTGGCGAGTAATTTGCGAAac < 1:489173/69‑1 (MQ=255) cATAAAATTCAGCGTGTCATTTGCCATATGCATTTCGAAGGACGGCGAGTGGCGAGTAATTTGCGAAac < 1:2827045/69‑1 (MQ=255) cATAAAATTCAGCGTGTCATTTGCCATATGCATTTCGAAGGACGGCGAGTGGCGAGTAATTTGCGAAac < 1:2755554/69‑1 (MQ=255) cATAAAATTCAGCGTGTCATTTGCCATATGCATTTCGAAGGACGGCGAGTGGCGAGTAATTTGCGAAac < 1:2299461/69‑1 (MQ=255) aTAAAATTCAGCGTGTCATTTGCCATATGCATTTCGAAGGACGGCGAGTGGCGAGTAATTTGCGAAac < 1:1577451/68‑1 (MQ=255) aTAAAATTCAGCGTGTCACTTGCCATATGCATTTCGAAGGACGGCGAGTGGCGAGTAATTTGCGAAacc > 1:1124219/1‑69 (MQ=255) tCAGCGTGTCACTTGCCATATGCATTTCGAAGGACGGCGAGTGGCGAGTAATTTGCGAAACCACCCGAt < 1:2958784/69‑1 (MQ=255) tCAGCGTGTCACTTGCCATATGCATTTCGAAGGACGGCGAGTGGCGAGTAATTTGCGAAACCACCCGAt < 1:3379070/69‑1 (MQ=255) tCAGCGTGTCACTTGCCATATGCATTTCGAAGGACGGCGAGTGGCGAGTAATTTGCGAAACCACCCGAt < 1:411532/69‑1 (MQ=255) tCAGCGTGTCACTTGCCATATGCATTTCGAAGGACGGCGAGTGGCGAGTAATTTGCGAAACCACCCGAt < 1:1267461/69‑1 (MQ=255) tCAGCGTGTCACTTGCCATATGCATTTCGAAGGACGGCGAGTGGCGAGTAATTTGCGAAACCACCCGAt < 1:1216820/69‑1 (MQ=255) tCAGCGTGTCACTTGCCATATGCATTTCGAAGGACGGCGAGTGGCGAGTAATTTGCGAAACCACCCGAt < 1:2903802/69‑1 (MQ=255) tCAGCGTGTCACTTGCCATATGCATTTCGAAGGACGGCGAGTGGCGAGTAATTTGCGAAACCACCCGAt < 1:1068130/69‑1 (MQ=255) | GTTCCTCTCCGTCCTGCGCCAGCAATGTTCCTTTCTCAAACGGCATAAAATTCAGCGTGTCACTTGCCATATGCATTTCGAAGGACGGCGAGTGGCGAGTAATTTGCGAAACCACCCGAT > minE/1153744‑1153863 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |