| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,155,567 | G→A | 48.6% | T128T (ACC→ACT) | astB ← | succinylarginine dihydrolase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,155,567 | 0 | G | A | 48.6% | 1.5 / 64.5 | 74 | T128T (ACC→ACT) | astB | succinylarginine dihydrolase |
| Reads supporting (aligned to +/- strand): ref base G (21/17); new base A (22/14); total (43/31) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.44e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
AACAGCGATTCAGTGACGGGCGCTTCCAGCGAACGGTGAAATTTATTGTTCAGGTTGGCAACGGTGAGATGCACTTTGCCATCCAGCGTATCGGCAGATGGCGCGATCGTTGCCGCATTGGCTACCCACAT > minE/1155505‑1155635 | aaCAGCGATTCAGTGACGGGCGCTTCCAGCGAACGGTGAAATTTATTGTTCAGGTTGGCAACGGTga < 1:976745/67‑1 (MQ=255)aaCAGCGATTCAGTGACGGGCGCTTCCAGCGAACGGTGAAATTTATTGTTCAGGTTGGCAACGGTga < 1:1090037/67‑1 (MQ=255)aaCAGCGATTCAGTGACGGGCGCTTCCAGCGAACGGTGAAATTTATTGTTCAGGTTGGCAACGGTga < 1:1094052/67‑1 (MQ=255)aaCAGCGATTCAGTGACGGGCGCTTCCAGCGAACGGTGAAATTTATTGTTCAGGTTGGCAACGGTga < 1:287788/67‑1 (MQ=255)aaCAGCGATTCAGTGACGGGCGCTTCCAGCGAACGGTGAAATTTATTGTTCAGGTTGGCAACGGTga < 1:287675/67‑1 (MQ=255)aaCAGCGATTCAGTGACGGGCGCTTCCAGCGAAAGGTGAAATTTATTGTTCAGGTTGGCAACGGTga < 1:3139770/67‑1 (MQ=255) aGCGATTCAGTGACGGGCGCTTCCAGCGAACGGTGAAATTTATTGTTCAGGTTGGCAACGGTGAGATg < 1:1002439/68‑1 (MQ=255) aGCGATTCAGTGACGGGCGCTTCCAGCGAACGGTGAAATTTATTGTTCAGGTTGGCAACGGTGAGATg < 1:170202/68‑1 (MQ=255) tGACGGGCGCTTCCAGCGAACGGTGAAATTTATTGTTCAGGTTGGCAACGGTGAGATGCACTTTGCCa < 1:100318/68‑1 (MQ=255) tGACGGGCGCTTCCAGCGAACGGTGAAATTTATTGTTCAGGTTGGCAACGGTGAGATGCACTTTGCCa < 1:3377223/68‑1 (MQ=255) tGACGGGCGCTTCCAGCGAACGGTGAAATTTATTGTTCAGGTTGGCAACGGTGAGATGCACTTTGCCa < 1:1085094/68‑1 (MQ=255) gggCGCTTTCAGCGAACGGTGAAATTTATTGTTCAGGTTGGCAACGGTGAGATGCACTTTGCCATCCAg > 1:2151030/1‑69 (MQ=255) gggCGCTTCCAGCGAACGGTGAAATTTATTGTTCAGGTTGGCAACGGTGAGATGCACTTTGCCATCCAg > 1:237653/1‑69 (MQ=255) gggCGCTTCCAGCGAACGGTGAAATTTATTGTTCAGGTTGGCAACGGTGAGATGCACTTTGCCATCCAg > 1:1699092/1‑69 (MQ=255) gggCGCTTCCAGCGAACGGTGAAATTTATTGTTCAGGTTGGCAACGGTGAGATGCACTTTGCCATCCAg > 1:280575/1‑69 (MQ=255) gggCGCTTCCAGCGAACGGTGAAATTTATTGTTCAGGTTGGCAACGGTGAGATGCACTTTGCCATCCAg > 1:536039/1‑69 (MQ=255) gggCGCTTCCAGCGAACGGTGAAATTTATTGTTCAGGTTGGCAACGGTGAGATGCACTTTGCCATCCAg > 1:869265/1‑69 (MQ=255) ggCGCTTCCAGCGAACGGTGAAATTTATTGTTCAGGTTGGCAACGGTGAGATGCACTTTGCCATCCAg > 1:847210/1‑68 (MQ=255) cgcTTCCAGCGAACGGTGAAATTTATTGTTCAGGTTGGCAACGGTGAGATGCACTTTGCCATCCAGCGt > 1:2692988/1‑69 (MQ=255) cgcTTCCAGCGAACGGTGAAATTTATTGTTCAGGTTGGCAACGGTGAGATGCACTTTGCCATCCAGCGt > 1:1083001/1‑69 (MQ=255) aCGGTGAAATTTATTGTTCAGGTTAGCAACAGTGAGATg > 1:1319573/1‑39 (MQ=37) aCGGTGAAATTTATTGTTCAGGTTAGCAACAGTGAGATGCACTTTGCCATCCAGc > 1:2461741/1‑55 (MQ=255) aCGGTGAAATTTATTGTTCAGGTTAGCAACAGTGAGATGCACTTTGCCATCCAGc > 1:2459009/1‑55 (MQ=255) aCGGTGAAATTTATTGTTCAGGTTAGCAACAGTGAGATGCACTTTGCCATCCAGc > 1:85290/1‑55 (MQ=255) aCGGTGAAATTTATTGTTCAGGTTAGCAACAGTGAGATGCACTTTGCCATCCAGc > 1:1295548/1‑55 (MQ=255) aCGGTGAAATTTATTGTTCAGGTTAGCAACAGTGAGATGCACTTTGCCATCCAGc > 1:753893/1‑55 (MQ=255) aCGGTGAAATTTATTGTTCAGGTTAGCAACAGTGAGATGCACTTTGCCATCCAGc > 1:263734/1‑55 (MQ=255) aCGGTGAAATTTATTGTTCAGGTTAGCAACAGTGAGATGCACTTTGCCATCCAGc > 1:2789781/1‑55 (MQ=255) aCGGTGAAATTTATTGTTCAGGTTAGCAACAGTGAGATGCACTTTGCCATCCAGc > 1:2668898/1‑55 (MQ=255) aCGGTGAAATTTATTGTTCAGGTTAGCAACAGTGAGATGCACTTTGCCATCCAGc > 1:3123713/1‑55 (MQ=255) aCGGTGAAATTTATTGTTCAGGTTAGCAACAGTGAGATGCACTTTGCCATCCAGc > 1:3306962/1‑55 (MQ=255) aCGGTGAAATTTATTGTTCAGGTTAGCAACAGTGAGATGCACTTTGCCATCCAGc > 1:2139938/1‑55 (MQ=255) aCGGTGAAATTTATTGTTCAGGTTAGCAACAGTGAGATGCACTTTGCCATCCAGc > 1:53117/1‑55 (MQ=255) aCGGTGAAATTTATTGTTCAGGTTAGCAACAGTGAGATGCACTTTGCCATCCAGc > 1:1885805/1‑55 (MQ=255) aCGGTGAAATTTATTGTTCAGGTTAGCAACAGTGAGATGCACTTTGCCATCCAGc > 1:1741090/1‑55 (MQ=255) aCGGTGAAATTTATTGTTCAGGTTAGCAACAGTGAGATGCACTTTGCCATCCAGc > 1:1740743/1‑55 (MQ=255) aCGGTGAAATTTATTGTTCAGGTTAGCAACAGTGAGATGCACTTTGCCATCCAGc > 1:927697/1‑55 (MQ=255) aCGGTGAAATTTATTGTTCAGGTTAGCAACAGTGAGATGCACTTTGCCATCCAGc > 1:1562547/1‑55 (MQ=255) aCGGTGAAATTTATTGTTCAGGTTAGCAACAGTGAGATGCACTTTGCCATCCAGc > 1:620687/1‑55 (MQ=255) aCGGTGAAATTTATTGTTCAGGTTAGCAACAGTGAGATGCACTTTGCCATCAAGc > 1:865424/1‑55 (MQ=255) aCGGTGAAATTTATTGTTCAGGTTAGCAACAGTGAGATGCACTTTGCCATACAGc > 1:1996615/1‑55 (MQ=255) aCGGAGAAATTTATTGTTCAGGTAAGCAACAGTgag > 1:1438659/1‑36 (MQ=19) tGAAATTTATTGTTCAGGTTGGCAACGGTGAGATGCACTTTGCCATCCAGCGTGTCGGCAGATGGcgcg < 1:3044006/69‑1 (MQ=255) tGAAATTTATTGTTCAGGTTGGCAACGGTGAGATGCACTTTGCCATCCAGCGTATCGGCAGATgg > 1:1799290/1‑65 (MQ=255) tGAAATTTATTGTTCAGGTTGGCAACGGTGAGATGCACTTTGCCATCCAGCGTATCGGCAGATGGcgcg > 1:2681862/1‑69 (MQ=255) tGAAATTTATTGTTCAGGTTGGCAACGGTGAGATGCACTTTGCCATCCAGCGTATCGGCAGATGGcgcg > 1:510346/1‑69 (MQ=255) tGAAATTTATTGTTCAGGTTGGCAACGGTGAGATGCACTTTGCCATCCAGCGTATCGGCAGATGGcgcg > 1:1411669/1‑69 (MQ=255) tGAAATATATTGTTCAGGTTGGCAACGGTGAGATGCACTTTGCCATCCAGCGTATCGGCAGATGGcgcg > 1:2638643/1‑69 (MQ=255) gAAATTTATTGTTCAGGTTGGCAACGGTGAGATGCACTTTGCCATCCAGCGTATCGGCAGATGGcgcg < 1:539100/68‑1 (MQ=255) ttATTGTTCAGGTTAGCAACAGTGAGATGCACTTTGCCATCCAGCGTATCGGCGGATGGCGCGATCGtt < 1:3082433/69‑1 (MQ=255) ttATTGTTCAGGTTAGCAACAGTGAGATGCACTTTGCCATCCAGCGTATCGGCGGATGGCGCGATCGtt < 1:2014640/69‑1 (MQ=255) ttATTGTTCAGGTTAGCAACAGTGAGATGCACTTTGCCATCCAGCGTATCGGCGGATGGCGCGATCGtt < 1:2168274/69‑1 (MQ=255) ttATTGTTCAGGTTAGCAACAGTGAGATGCACTTTGCCATCCAGCGTATCGGCGGATGGCGCGATCGtt < 1:2241927/69‑1 (MQ=255) ttATTGTTCAGGTTAGCAACAGTGAGATGCACTTTGCCATCCAGCGTATCGGCGGATGGCGCGATCGtt < 1:3244358/69‑1 (MQ=255) ttATTGTTCAGGTTAGCAACAGTGAGATGCACTTTGCCATCCAGCGTATCGGCGGATGGCGCGATCGtt < 1:2256453/69‑1 (MQ=255) ttATTGTTCAGGTTAGCAACAGTGAGATGCACTTTGCCATCCAGCGTATCGGCGGATGGCGCGATCGtt < 1:66746/69‑1 (MQ=255) ttATTGTTCAGGTTAGCAACAGTGAGATGCACTTTGCCATCCAGCGTATCGGCGGATGGCGCGATCGtt < 1:2313554/69‑1 (MQ=255) ttATTGTTCAGGTTAGCAACAGTGAGATGCACTTTGCCATCCAGCGTATCGGCGGATGGCGCGATCGtt < 1:288434/69‑1 (MQ=255) ttATTGTTCAGGTTAGCAACAGTGAGATGCACTTTGCCATCCAGCGTATCGGCGGATGGCGCGATCGtt < 1:2395137/69‑1 (MQ=255) ttATTGTTCAGGTTAGCAACAGTGAGATGCACTTTGCCATCCAGCGTATCGGCGGATGGCGCGATCGtt < 1:2423392/69‑1 (MQ=255) ttATTGTTCAGGTTAGCAACAGTGAGATGCACTTTGCCATCCAGCGTATCGGCGGATGGCGCGATCGtt < 1:2535482/69‑1 (MQ=255) ttATTGTTCAGGTTAGCAACAGTGAGATGCACTTTGCCATCCAGCGTATCGGCGGATGGCGCGATCGtt < 1:2761874/69‑1 (MQ=255) ttATTGTTCAGGTTAGCAACAGTGAGATGCACTTTGCCATCCAGCGTATCGGCGGATGGCGCGATCGtt < 1:266340/69‑1 (MQ=255) gTTCAGGTTGGCAACGGTGAGATGCACTTTGCCATCCAGCGTATCGGCAGATGGCGCGATCGTTGCCGc < 1:256481/69‑1 (MQ=255) ggCAACGGTGAGATGCACTTTGCCATCCAGCGTATCGTCAGATGGCGCGATCGTTGCCGCATTGGCt > 1:2675510/1‑67 (MQ=255) ggCAACGGTGAGATGCACTTTGCCATCCAGCGTATCGGCAGATGGCGCGATCGTTGCCGCATTGGCTa > 1:3124965/1‑68 (MQ=255) ggCAACGGTGAGATGCACTTTGCCATCCAGCGTATCGGCAGATGGCGCGATCGTTGCCGCATTGGCTa > 1:2874055/1‑68 (MQ=255) ggCAACGGTGAGATGCACTTTGCCATCCAGCGTATCGGCAGATGGCGCGATCGTTGCCGCATTGGCTa > 1:1620976/1‑68 (MQ=255) ggCAACGGTGAGATGCACTTTGCCATCCAGCGTATCGGCAGATGGCGCGATCGTTGCCGCATTGGCTa > 1:945170/1‑68 (MQ=255) ggCAACGGTGAGATGCACTTTGCCATCCAGCGTATCGGCAGATGGCGCGATCGTTGCCGCATTGGCTa > 1:1014584/1‑68 (MQ=255) ggCAACGGTGAGATGCACTTTGCCATCCAGCGTATCGGCAGATGGCGAGATCGTTGCCGCATTGGCTa > 1:930720/1‑68 (MQ=255) aaCGGTGAGATGCACTTTGCCATCCAGCGTATCGGCAGATGGCGCGATCGTTGCCGCATTGGCTACCca < 1:1169052/69‑1 (MQ=255) aaCGGTGAGATGCACTTTGCCATCCAGCGTATCGGCAGATGGCGCGATCGTTGCCGCATTGGCTACCca < 1:2517697/69‑1 (MQ=255) aaCGGTGAGATGCACTTTGCCATCCAGCGTATCGGCAGATGGCGCGATCGTTGCCGCATTGGCTACCca < 1:2135023/69‑1 (MQ=255) ggTGAGATGCACTTTGCCATCCAGCGTATCGGCAGATGGCGCGATCGTTGCCGCATTGGCTACCCACAt > 1:2106191/1‑69 (MQ=255) | AACAGCGATTCAGTGACGGGCGCTTCCAGCGAACGGTGAAATTTATTGTTCAGGTTGGCAACGGTGAGATGCACTTTGCCATCCAGCGTATCGGCAGATGGCGCGATCGTTGCCGCATTGGCTACCCACAT > minE/1155505‑1155635 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |