| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,156,744 | A→G | 44.1% | L228L (TTG→CTG) | astD ← | succinylglutamic semialdehyde dehydrogenase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,156,744 | 0 | A | G | 44.1% | 9.3 / 45.5 | 59 | L228L (TTG→CTG) | astD | succinylglutamic semialdehyde dehydrogenase |
| Reads supporting (aligned to +/- strand): ref base A (12/21); new base G (12/14); total (24/35) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.94e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.74e-01 | |||||||||||
ACCGCCCATCTCAAGGGCGAGAATTTTCTCCGGCTGACCGGAGAGCTGGCGATGCAACTGGTAGCCTGTATTGGCGCTACCGGTAAACAGCAAACCGTCGAGATCCTCCAGCGCACTCAGCGC > minE/1156688‑1156810 | aCCGCCCATCTCAAGGGCGAGAATTTTCTCCGGCTGACCGGAGAGCTGGCGATGCAACTGGTAGCCTGt > 1:947890/1‑69 (MQ=255)aCCGCCCATCTCAAGGGCGAGAATTTTCTCCGGCTGACCGGAGAGCTGGCGATGCAACTGGTAGCCTGt > 1:2422717/1‑69 (MQ=255) tcAAGGGCGAGAATTTTCTCCGGCTGACCGGAGAGCTGGCGATGCAACTGGTAGCCTg > 1:2070120/1‑58 (MQ=255) tcAAGGGCGAGAATTTTCTCCGGCTGACCGGAGAGCTGGCGATGCAACTGGTAGCCTGTATTGGCGCTa > 1:3044311/1‑69 (MQ=255) tcAAGGGCGAGAATTTTCTCCGGCTGACCGGAGAGCTGGCGATGCAACTGGTAGCCTGTATTGGCGCTa > 1:1846618/1‑69 (MQ=255) ggCGAGAATTTTCTCCGGCTGACCGGAGAGCTGGCGATGCAACTGGTAGCCTGTATTGGCGCt < 1:874323/63‑1 (MQ=255) ggCGAGAATTTTCTCCGGCTGACCGGAGAGCTGGCGATGCAACTGGTAGCCTGTATTGGCGCt < 1:1546206/63‑1 (MQ=255) gCGAGAATTTTCTCCGGCTGACCGGAGAGCTGGCGATGCAACTGGTAGCCTGTATTGGCGCTACCGGTa < 1:1264815/69‑1 (MQ=255) gCGAGAATTTTCTCCGGCTGACCGGAGAGCTGGCGATGCAACTGGTAGCCTGTATTGGCGCTACCGGTa < 1:3429695/69‑1 (MQ=255) gCGAGAATTTTCTCCGGCTGACCGGAGAGCTGGCGATGCAACTGGTAGCCTGTATTGGCGCTACCGGTa < 1:29931/69‑1 (MQ=255) gCGAGAATTTTCTCCGGCTGACCGGAGAGCTGGCGATGCAACTGGTAGCCTGTATTGGCGCTACCGGTa < 1:2976295/69‑1 (MQ=255) gCGAGAATTTTCTCCGGCTGACCGGAGAGCTGGCGATGCAACTGGTAGCCTGTATTGGCGCTACCGGTa < 1:2816779/69‑1 (MQ=255) gCGAGAATTTTCTCCGGCTGACCGGAGAGCTGGCGATGCAACTGGTAGCCTGTATTGGCGCTACCGGTa < 1:218615/69‑1 (MQ=255) gCGAGAATTTTCTCCGGCTGACCGGAGAGCTGGCGATGCAACTGGTAGCCTGTATTGGCGCTACCGGTa < 1:1979074/69‑1 (MQ=255) gCGAGAATTTTCTCCGGCTGACCGGAGAGCTGGCGATGCAACTGGTAGCCTGTATTGGCGCTACCGGTa < 1:1141960/69‑1 (MQ=255) cGAGAATTTTCTCCGGCTGACCGGAGAGCTGGCGATGCAACTGGTAGCCTGTATTGGCGCTACCGGTa < 1:1873004/68‑1 (MQ=255) cGAGAATTTTCTCCGGCTGACCGGAGAGCTGGCGATGCAACTGGTAGCCTGTATTGGCGCTACCGGTa < 1:897004/68‑1 (MQ=255) agaATTTTCTCCGGCTGACCGGAAAGCTGGCGATGCAGCTGGTAGCCGGTATTGGCGCTGCCGGTAAAc < 1:2101168/69‑1 (MQ=255) agaATTTTCTCCGGCTGACCGGAAAGCTGGCGATGCAGCTGGTAGCCGGTATTGGCGCTGCCGGTAAAc < 1:2984110/69‑1 (MQ=255) agaATTTTCTCCGGCTGACCGGAAAGCTGGCGATGCAGCTGGTAGCCGGTATTGGCGCTGCCGGTAAAc < 1:1364929/69‑1 (MQ=255) agaATTTTCTCCGGCTGACCGGAAAGCTGGCGATGCAGCTGGTAGCCGGTATTGGCGCTGCCGGTAAAc < 1:3239420/69‑1 (MQ=255) agaATTTTCTCCGGCTGACCGGAAAGCTGGCGATGCAGCTGGTAGCCGGTATTGGCGCTGCCGGTAAAc < 1:1585686/69‑1 (MQ=255) agaATTTTCTCCGGCTGACCGGAAAGCTGGCGATGCAGCTGGTAGCCGGTATTGGCGCTGCCGGTAAAc < 1:341402/69‑1 (MQ=255) agaATTTTCTCCGGCTGACCGGAAAGCTGGCGATGCAGCTGGTAGCCGGTATTGGCGCTGCCGGTAAAc < 1:2182781/69‑1 (MQ=255) agaATTTTCTCCGGCTGACCGGAAAGCTGGCGATGCAGCTGGTAGCCGGTATTGGCGCTGCCGGTAAAc < 1:2149691/69‑1 (MQ=255) agaATTTTCTCCGGCTGACCGGAAAGCTGGCGATGCAGCTGGTAGCCGGTATTGGCGCTGCCGGTAAAc < 1:2123020/69‑1 (MQ=255) agaATTTTCTCCGGCTGACCGGAAAGCTGGCGATGCAGCTGGTAGCCGGTATTGGCGCTGCCGGTAAAc < 1:2056916/69‑1 (MQ=255) agaATTTTCTCCGGCTGACCGGAAAGCTGGCGATGCAGCTGGTAGCCGGTATTGGCGCTGCCGGTAAAc < 1:2053038/69‑1 (MQ=255) agaATTTTCTCCGGCTGACCGGAAAGCTGGCGATGCAGCTGGTAGCCGGTATTGGCGCTGCCGGTAAAc < 1:1341658/69‑1 (MQ=255) agaATTTTCTCCGGCTGACCGGAAAGCTGGCGATGCAGCTGGTAGCCGGTATTGGCGCTGCCGGTAAAc < 1:600942/69‑1 (MQ=255) agaATTTTCTCCGGCTGACCGGAAAGCTGGCGATGCAGCTGGTAGCCGGTATTGGCGCTGCCGGTAAAc < 1:696457/69‑1 (MQ=255) ttttCTCCGGCTGACCGGAGAGCTGGCGATGCAACTGGTAGCCTGTATTGGCGCTACCGGTAAACAGCa > 1:1654251/1‑69 (MQ=255) tttCTCCGGCTGACCGGAGAGCTGGCGATGCAACTGGTAGCCTGTATTGGCGCTACCGGTAAACAGCaa < 1:3255002/69‑1 (MQ=255) tttCTCCGGCTGACCGGAGAGCTGGCGATGCAACTGGTAGCCTGTATTGGCGCTACCGGTAAACAGCa < 1:2838037/68‑1 (MQ=255) tttCTCCGGCTGACCGGAGAGCTGGCGATGCAACTGGTAGCCTGTATTGGCGCTACCGGTAAACAGCa < 1:2889671/68‑1 (MQ=255) tttCTCCGGCTGACCGGAGAGCTGGCGATGCAACTGGTAGCCTGTATTGGCGCTACCGGTAAACAGCa < 1:262556/68‑1 (MQ=255) ttCTCCGGCTGACCGGAGAGCTGGCGATGCAACTGGTAGCCTGTATTGGCGCTACCGGTAAACAGCaaa > 1:1303754/1‑69 (MQ=255) tGACCGGAGAGCTGGCGATGCAACTGGTAGCCTTTATTGGCGCTACCGGTAAACAGCAAACCGTCgaga < 1:1942759/69‑1 (MQ=255) tGACCGGAGAGCTGGCGATGCAACTGGTAGCCTGTATTGGCGCTACCGGTAAACAGCAAACCGTCgaga < 1:3375486/69‑1 (MQ=255) tGACCGGAGAGCTGGCGATGCAACTGGTAGCCTGTATTGGCGCTACCGGTAAACAGCAAACCGTCgaga < 1:3145888/69‑1 (MQ=255) tGACCGGAGAGCTGGCGATGCAACTGGTAGCCTGTATTGGCGCTACCGGTAAACAGCAAACCGTCgaga < 1:2493957/69‑1 (MQ=255) gACCGGAGAGCTGGCGATGCAACTGGTAGCCTGTATTGGCTCTACCGGTAAACAGCAAACCGTCgaga < 1:780244/68‑1 (MQ=255) cGGAAAGCTGGCGATGCAGCTGGTAGCCGGTATTGGCGCTGCCGGTAAACAGCAAGCCATCgag > 1:3254600/1‑64 (MQ=255) cGGAAAGCTGGCGATGCAGCTGGTAGCCGGTATTGGCGCTGCCGGTAAACAGCAAGCCATCgag > 1:1714821/1‑64 (MQ=255) cGGAAAGCTGGCGATGCAGCTGGTAGCCGGTATTGGCGCTGCCGGTAAACAGCAAGCCATCgag > 1:1927370/1‑64 (MQ=255) cGGAAAGCTGGCGATGCAGCTGGTAGCCGGTATTGGCGCTGCCGGTAAACAGCAAGCCATCgag > 1:927418/1‑64 (MQ=255) cGGAAAGCTGGCGATGCAGCTGGTAGCCGGTATTGGCGCTGCCGGTAAACAGCAAGCCATCgag > 1:3334779/1‑64 (MQ=255) cGGAAAGCTGGCGATGCAGCTGGTAGCCGGTATTGGCGCTGCCGGTAAACAGCAAGCCATCgag > 1:2339395/1‑64 (MQ=255) cGGAAAGCTGGCGATGCAGCTGGTAGCCGGTATTGGCGCTGCCGGTAAACAGCAAGCCATCgag > 1:3125445/1‑64 (MQ=255) cGGAAAGCTGGCGATGCAGCTGGTAGCCGGTATTGGCGCTGCCGGTAAACAGCAAGCCATCgag > 1:1355029/1‑64 (MQ=255) cGGAAAGCTGGCGATGCAGCTGGTAGCCGGTATTGGCGCTGCCGGTAAACAGCAAGCCATCgag > 1:2544320/1‑64 (MQ=255) cGGAAAGCTGGCGATGCAGCTGGTAGCCGGTATTGGCGCTGCCGGTAAACAGCAAGCCATCgag > 1:1367178/1‑64 (MQ=255) cGGAAAGCTGGCGATGCAGCTGGTAGCCGGTATTGGCGCTGCCGGTAAACAGCAAGCCATCgag > 1:2724894/1‑64 (MQ=255) cGGAAAGCTGGCGATGCAGCTGGTAGCCGGTATTGGCGCTGCCGGTAAACAGCAAGCCATCgag > 1:1557868/1‑64 (MQ=255) cAACTGGTAGCCTGTATTGGCGCTACCGGTAAACAGCAAACCGTCGAGATCCTCCAGCGCACTCAgcgc > 1:1858909/1‑69 (MQ=255) cAACTGGTAGCCTGTATTGGCGCTACCGGTAAACAGCAAACCGTCGAGATCCTCCAGCGCACTCAgcgc > 1:2003896/1‑69 (MQ=255) cAACTGGTAGCCTGTATTGGCGCTACCGGTAAACAGCAAACCGTCGAGATCCTCCAGCGCACTCAgcgc > 1:1586423/1‑69 (MQ=255) cAACTGGTAGCCTGTATTGGCGCTACCGGTAAACAGCAAACCGTCGAGATCCTCCAGCGCACTCAgcgc > 1:335400/1‑69 (MQ=255) cAACTGGTAGCCTGTATTGGCGCTACCGGTAAACAGCAAACCGTCGAGATCCTCCAGCGCACTCAgcgc > 1:1718775/1‑69 (MQ=255) | ACCGCCCATCTCAAGGGCGAGAATTTTCTCCGGCTGACCGGAGAGCTGGCGATGCAACTGGTAGCCTGTATTGGCGCTACCGGTAAACAGCAAACCGTCGAGATCCTCCAGCGCACTCAGCGC > minE/1156688‑1156810 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |