| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 17,516 | G→C | 22.5% | V87V (GTG→GTC) | nhaR → | DNA‑binding transcriptional activator |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 17,516 | 0 | G | C | 22.5% | 182.2 / 46.5 | 102 | V87V (GTG→GTC) | nhaR | DNA‑binding transcriptional activator |
| Reads supporting (aligned to +/- strand): ref base G (46/33); new base C (7/16); total (53/49) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.15e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.25e-01 | |||||||||||
Only 100 of 113 total aligned reads displayed.
CTGGGAGAACTGGTCTATCGCTATGCCGATAAAATGTTCACCTTAAGCCAGGAAATGCTGGATATTGTGAACTATCGCAAAGAATCCAATTTATTGTTTGACGTTGGCGTGGCTGATGCACTTTCCAAACGCCTG > minE/17448‑17582 | cTGGGAGAACTGGTCTATCGCTATGCCGATAAAATGTTCACCTTAAGCCAGGAAATGCTGGATATtgtg < 1:2861204/69‑1 (MQ=255)cTGGGAGAACTGGTCTATCGCTATGCCGATAAAATGTTCACCTTAAGCCAGGAAATGCTGGATATtgtg < 1:1597056/69‑1 (MQ=255)cTGGGAGAACTGGTCTATCGCTATGCCGATAAAATGTTCACCTTAAGCCAGGAAATGCTGGATATtgtg < 1:768926/69‑1 (MQ=255)cTGGGAGAACTGGTCTATCGCTATGCCGATAAAATGTTCACCTTAAGCCAGGAAATGCTGGATATtgtg < 1:3374908/69‑1 (MQ=255)cTGGGAGAACTGGTCTATCGCTATGCCGATAAAATGTTCACCTTAAGCCAGGAAATGCTGGATATtgtg < 1:1781993/69‑1 (MQ=255) tATCGCTATGCCGATAAAATGTTCACCTTAAGCCAGGAAATGCTGGATATTGTGAACTATCGCAAAGaa > 1:1303360/1‑69 (MQ=255) tCGCTATGCCGATAAAATGTTCACCTTAAGCCAGGAAATGCTGGATATTGTGAACTATCGCAAAGAAt > 1:2912807/1‑68 (MQ=255) gCTATGCCGATAAAATGTTCACCTTAAGCCAGGAAATGCTGGATATTGTGAACTATCGCAAAGAATCCa > 1:904486/1‑69 (MQ=255) gCTATGCCGATAAAATGTTCACCTTAAGCCAGGAAATGCTGGATATTGTGAACTATCGCAAAGAATCCa > 1:759494/1‑69 (MQ=255) gCTATGCCGATAAAATGTTCACCTTAAGCCAGGAAATGCTGGATATTGTGAACTATCGCAAAGAATCCa > 1:654680/1‑69 (MQ=255) gCTATGCCGATAAAATGTTCACCTTAAGCCAGGAAATGCTGGATATTGTGAACTATCGCAAAGAATCCa > 1:1321340/1‑69 (MQ=255) tGCCGATAAAATGTTCACCTTAAGCCAGGAAATGCTGGATATTGTGAACTATCGCAAAGAATCCAAttt > 1:2495164/1‑69 (MQ=255) tGCCGATAAAATGTTCACCTTAAGCCAGGAAATGCTGGATATTGTGAACTATCGCAAAGAATCCAAttt > 1:282697/1‑69 (MQ=255) tGCCGATAAAATGTTCACCTTAAGCCAGGAAATGCTGGATATTGTGAACTATCGCAAAGAATCCAAttt > 1:2745596/1‑69 (MQ=255) tGCCGATAAAATGTTCACCTTAAGCCAGGAAATGCTGGATATTGTGAACTATCGCAAAGAATCCAAttt > 1:321514/1‑69 (MQ=255) tGCCGATAAAATGTTCACCTTAAGCCAGGAAATGCTGGATATTGTGAACTATCGCAAAGAATCCAAttt > 1:3221010/1‑69 (MQ=255) tGCCGATAAAATGTTCACCTTAAGCCAGGAAATGCTGGATATTGTGAACTATCGCAAAGAATCCAAttt > 1:2440175/1‑69 (MQ=255) aaaaTGTTCACCTTAAGCCAGGAAATGCTGGATATTGTGAACTATCGCAAAGAATCCAATTTATTGttt < 1:581186/69‑1 (MQ=255) aaaaTGTTCACCTTAAGCCAGGAAATGCTGGATATTGTGAACTATCGCAAAGAATCCAATTTATTGttt < 1:911872/69‑1 (MQ=255) aaaaTGTTCACCTTAAGCCAGGAAATGCTGGATATTGTGAACTATCGCAAAGAATCCAATTTATTGttt < 1:2421095/69‑1 (MQ=255) aTGTTCACCTTAAGCCAGGAAATGCTGGATATTGTGAACTATCGCAAAGAATCCAATTTATTGTTTGAc > 1:10182/1‑69 (MQ=255) aTGTTCACCTTAAGCCAGGAAATGCTGGATATTGTGAACTATCGCAAAGAATCCAATTTATTGTTTGAc > 1:2691554/1‑69 (MQ=255) aTGTTCACCTTAAGCCAGGAAATGCTGGATATTGTGAACTATCGCAAAGAATCCAATTTATTGTTTGAc > 1:3369960/1‑69 (MQ=255) aTGTTCACCTTAAGCCAGGAAATGCTGGATATTGTGAACTATCGCAAAGAATCCAATTTATTGTTTGAc > 1:2747133/1‑69 (MQ=255) aTGTTCACCTTAAGCCAGGAAATGCTGGATATTGTGAACTATCGCAAAGAATCCAATTTATTGTTTGAc > 1:296940/1‑69 (MQ=255) aTGTTCACCTTAAGCCAGGAAATGCTGGATATTGTGAACTATCGCAAAGAATCCAATTTATTGTTTGAc > 1:2731951/1‑69 (MQ=255) aTGTTCACCTTAAGCCAGGAAATGCTGGATATTGTGAACTATCGCAAAGAATCCAATTTATTGTTTGAc > 1:450359/1‑69 (MQ=255) aTGTTCACCTTAAGCCAGGAAATGCTGGATATTGTGAACTATCGCAAAGAATCCAATTTATTGTTTGAc > 1:454430/1‑69 (MQ=255) aTGTTCACCTTAAGCCAGGAAATGCTGGATATTGTGAACTATCGCAAAGAATCCAATTTATTGTTTGAc > 1:483793/1‑69 (MQ=255) aTGTTCACCTTAAGCCAGGAAATGCTGGATATTGTGAACTATCGCAAAGAATCCAATTTATTGTTTGAc > 1:904500/1‑69 (MQ=255) aTGTTCACCTTAAGCCAGGAAATGCTGGATATTGTGAACTATCGCAAAGAATCCAATTTATTGTTTGAc > 1:982055/1‑69 (MQ=255) aTGTTCACCTTAAGCCAGGAAATGCTGGATATTGTGAACTATCGCAAAGAATCCAATTTATTGTTTGAc > 1:1592777/1‑69 (MQ=255) aTGTTCACCTTAAGCCAGGAAATGCTGGATATTGTGAACTATCGCAAAGAATCCAATTTATTGTTTGAc > 1:1365947/1‑69 (MQ=255) aTGTTCACCTTAAGCCAGGAAATGCTGGATATTGTGAACTATCGCAAAGAATCCAATTTATTGTTTGAc > 1:1791557/1‑69 (MQ=255) aTGTTCACCTTAAGCCAGGAAATGCTGGATATTGTGAACTATCGCAAAGAATCCAATTTATTGTTTGAc > 1:1245176/1‑69 (MQ=255) ttCACCTTAAGCCAGGAAATGCTGGATATTGTGAACTATCGCAAAGAATCCAATTTATTGt < 1:1163663/61‑1 (MQ=255) ttCACCTTAAGCCAGGAAATGCTGGATATTGTGAACTATCGCAAAGAATCCAATTTATTGt < 1:1380311/61‑1 (MQ=255) ttCACCTTAAGCCAGGAAATGCTGGATATTGTGAACTATCGCAAAGAATCCAATTTATTGt < 1:3151314/61‑1 (MQ=255) ttCACCTTAAGCCAGGAAATGCTGGATATTGTGAACTATCGCAAAGAATCCAATTTATTGt < 1:473756/61‑1 (MQ=255) ttCACCTTAAGCCAGGAAATGCTGGATATTGTGAACTATCGCAAAGAATCCAATTTATTGt < 1:3213289/61‑1 (MQ=255) ttCACCTTAAGCCAGGAAATGCTGGATATTGTGAACTATCGCAAAGAATCCAATTTATTGt < 1:1514369/61‑1 (MQ=255) ttCACCTTAAGCCAGGAAATGCTGGATATTGTGAACTATCGCAAAGAATCCAATTTATTGt < 1:1882/61‑1 (MQ=255) ttCACCTTAAGCCAGGAAATGCTGGATATT‑TGAACTATCGCAAAGAATCCAATTTATTGt < 1:1095310/60‑1 (MQ=255) ttCACCTTAAGCCAGGAAATCCTGGATATTGTGAACTATCGCAAAGAATCCAATTTATTGt < 1:605616/61‑1 (MQ=255) cACCTTAAGCCAGGAAATGCTGGATATTGTGAACTATCGCAAAGAATCCAATTTATTGTTTGACGTTgg > 1:3362203/1‑69 (MQ=255) cACCTTAAGCCAGGAAATGCTGGATATTGTGAACTATCGCAAAGAATCCAATTTATTGTTTGACGTTgg > 1:752650/1‑69 (MQ=255) cACCTTAAGCCAGGAAATGCTGGATATTGTGAACTATCGCAAAGAATCCAATTTATTGTTTGACGTTgg < 1:2812594/69‑1 (MQ=255) cACCTTAAGCCAGGAAATGCTGGATATTGTGAACTATCGCAAAGAATCCAATTTATTGTTTGACGTTgg > 1:1538700/1‑69 (MQ=255) cACCTTAAGCCAGGAAATGCTGGATATTGTGAACTATCGCAAAGAATCCAATTTATTGTTTGACGTTgg > 1:3102917/1‑69 (MQ=255) cACCTTAAGCCAGGAAATGCTGGATATTGTGAACTATCGCAAAGAATCCAATTTATTGTTTGACGTTgg > 1:246144/1‑69 (MQ=255) cACCTTAAGCCAGGAAATGCTGGATATTGTGAACTATCGCAAAGAATCCAATTTATTGTTTGACGTTgg < 1:351558/69‑1 (MQ=255) cACCTTAAGCCAGGAAATGCTGGATATTGTGAACTATCGCAAAGAATCCAATTTATTGTTTGACGTTgg > 1:383661/1‑69 (MQ=255) cACCTTAAGCCAGGAAATGCTGGATATTGTGAACTATCGCAAAGAATCCAATTTATTGTTTGACGTTgg > 1:241261/1‑69 (MQ=255) cACCTTAAGCCAGGAAATGCTGGATATTGTGAACTATCGCAAAGAATCCAATTTATTGTTTGACGTTgg > 1:1358114/1‑69 (MQ=255) cACCTTAAGCCAGGAAATGCTGGATATTGTGAACTATCGCAAAGAATCCAATTTATTGTTTGACGTTgg > 1:237336/1‑69 (MQ=255) cACCTTAAGCCAGGAAATGCTGGATATTGTGAACTATCGCAAAGAATCCAATTTATTGTTTGACGTTgg > 1:1352163/1‑69 (MQ=255) cACCTTAAGCCAGGAAATGCTGGATATTGTGAACTATCGCAAAGAATCCAATTTATTGTTTGACGTTgg > 1:1344/1‑69 (MQ=255) cACCTTAAGCCAGGAAATGCTGGATATTGTGAACTATCGCAAAGAATCCAATTTATTGTTTGACGTTgg > 1:4963/1‑69 (MQ=255) cACCTTAAGCCAGGAAATGCTGGATATTGTGAACTATCGCAAAGAATCCAATTTATTGTTTGACGTTgg > 1:534776/1‑69 (MQ=255) cACCTTAAGCCAGGAAATGCAGGATATTGTGAACTATCGCAAAGAATCCAATTTATTGTTTGACGTTgg > 1:1868314/1‑69 (MQ=255) aaGCCAGGAAATGCTGGATATCGTCAACTATCGCAAAGAGTCCAACTTATTGTTTGATGTTggtg > 1:2619558/1‑63 (MQ=255) aaGCCAGGAAATGCTGGATATCGTCAACTATCGCAAAGAGTCCAACTTATTGTTTGATGTTGgtgt > 1:1442770/1‑66 (MQ=255) aaGCCAGGAAATGCTGGATATCGTCAACTATCGCAAAGAGTCCAACTTATTGTTTGATGTTGgtgt > 1:1600373/1‑66 (MQ=255) aaGCCAGGAAATGCTGGATATCGTCAACTATCGCAAAGAGTCCAACTTATTGTTTGATGTTGgtgt > 1:2369861/1‑66 (MQ=255) aaGCCAGGAAATGCTGGATATCGTCAACTATCGCAAAGAGTCCAACTTATTGTTTGATGTTGgtgt > 1:1649069/1‑66 (MQ=255) aaGCCAGGAAATGCTGGATATCGTCAACTATCGCAAAGAGTCCAACTTATTGTTTGATGTTGgtgt > 1:2420452/1‑66 (MQ=255) gCCAGGAAATGCTGGATATTGTGAACTATCGCAAAGAATCCAATTTATTGTTTGACGTTGGCGTGGCt < 1:106029/68‑1 (MQ=255) aGGAAATGCTGGATATCGTCAACTATCGCAAAGAGTCCAACTTa < 1:3389723/44‑1 (MQ=25) aGGAAATGCTGGATATCGTCAACTATCGCAAAGAGTCCAACTTa < 1:1928140/44‑1 (MQ=25) aGGAAATGCTGGATATCGTCAACTATCGCAAAGAGTCCAACTTa < 1:514980/44‑1 (MQ=25) aGGAAATGCTGGATATCGTCAACTATCGCAAAGAGTCCAACTTa < 1:2238413/44‑1 (MQ=25) aGGAAATGCTGGATATCGTCAACTATCGCAAAGAGTCCAACTTa < 1:2437220/44‑1 (MQ=25) aGGAAATGCTGGATATCGTCAACTATCGCAAAGAGTCCAACTTa < 1:3417086/44‑1 (MQ=25) aGGAAATGCTGGATATCGTCAACTATCGCAAAGAGTCCAACTTa < 1:3087142/44‑1 (MQ=25) aGGAAATGCTGGATATCGTCAACTATCGCAAAGAGTCCAACTTa < 1:1846245/44‑1 (MQ=25) aGGAAATGCTGGATATCGTCAACTATCGCAAAGAGTCCAACTTa < 1:876141/44‑1 (MQ=25) aGGAAATGCTGGATATCGTCAACTATCGCAAAGAGTCCAACTTa < 1:1764042/44‑1 (MQ=25) aGGAAATGCTGGATATCGTCAACTATCGCAAAGAGTCCAACTTa < 1:662040/44‑1 (MQ=25) aGGAAATGCTGGATATCGTCAACTATCGCAAAGAGTCCAACTTa < 1:3214957/44‑1 (MQ=25) aGGAAATGCTGGATATCGTCAACTATCGCAAAGAGTCCAACTTa < 1:125542/44‑1 (MQ=25) aGGAAATGCTGGATATCGTCAACTATCGCAAAGAGTCCAACTTa < 1:3138493/44‑1 (MQ=25) ggAAATGCTGGATATTGTGAACTATCGCAAAGAATCCAATTTATTGTTTGACGTTGGCGTGGCTTATGc < 1:933011/69‑1 (MQ=255) ggAAATGCTGGATATTGTGAACTATCGCAAAGAATCCAATTTATTGTTTGACGTTGGCGTGGCTGATGc < 1:929411/69‑1 (MQ=255) ggAAATGCTGGATATTGTGAACTATCGCAAAGAATCCAATTTATTGTTTGACGTTGGCGTGGCTGATGc < 1:2041650/69‑1 (MQ=255) ggAAATGCTGGATATTGTGAACTATCGCAAAGAATCCAATTTATTGTTTGACGTTGGCGTGGCTGATGc < 1:3120778/69‑1 (MQ=255) ggAAATGCTGGATATTGTGAACTATCGCAAAGAATCCAATTTATTGTTTGACGTTGGCGTGGCTGATGc < 1:2941013/69‑1 (MQ=255) ggAAATGCTGGATATTGTGAACTATCGCAAAGAATCCAATTTATTGTTTGACGTTGGCGTGGCTGATGc < 1:2802791/69‑1 (MQ=255) ggAAATGCTGGATATTGTGAACTATCGCAAAGAATCCAATTTATTGTTTGACGTTGGCGTGGCTGATGc < 1:1796518/69‑1 (MQ=255) ggAAATGCTGGATATTGTGAACTATCGCAAAGAATCCAATTTATTGTTTGACGTTGGCGTGGCTGATGc < 1:260459/69‑1 (MQ=255) ggAAATGCTGGATATTGTGAACTATCGCAAAGAATCCAATTTATTGTTTGACGTTGGCGTGGCTGATGc < 1:2409047/69‑1 (MQ=255) gAAATGCTGGATATTGTGAACTATCGCAAAGAATCCAATTTATTGTTTGACGTTGGCGTGGCTGATGc < 1:1047464/68‑1 (MQ=255) ggATATTGTGAACTATCGCAAAGAATCCAATTTATTGTTTGACGTTGGCGTGGCTGATGCACTTTCCa < 1:1307422/68‑1 (MQ=255) ggATATTGTGAACTATCGCAAAGAATCCAATTTATTGTTTGACGTTGGCGTGGCTGATGCACTTTCCa < 1:2740315/68‑1 (MQ=255) ggATATTGTGAACTATCGCAAAGAATCCAATTTATTGTTTGACGTTGGCGTGGCTGATGCACTTTCCa < 1:2374750/68‑1 (MQ=255) ggATATTGTGAACTATCGCAAAGAATCCAATTTATTGTTTGACGTTGGCGTGGCTGATGCACTTTCCa < 1:2248130/68‑1 (MQ=255) ggATATTGTGAACTATCGCAAAGAATCCAATTTATTGTTTGACGTTGGCGTGGCTGATGCACTTTCCa < 1:2244093/68‑1 (MQ=255) gtgAACTATCGCAAAGAATCCAATTTATTGTTTGACGTTGGCGTGGCt > 1:1698521/1‑48 (MQ=255) gtgAACTATCGCAAAGAATCCAATTTATTGTTTGACGTTGGCGTGGCt > 1:753083/1‑48 (MQ=255) gtgAACTATCGCAAAGAATCCAATTTATTGTTTGACGTTGGCGTGGCTGATGCACTTTCCAAACGCCt > 1:640259/1‑68 (MQ=255) gtgAACTATCGCAAAGAATCCAATTTATTGTTTGACGTTGGCGTGGCTGATGCACTTTCCAAACGCCTg > 1:10599/1‑69 (MQ=255) | CTGGGAGAACTGGTCTATCGCTATGCCGATAAAATGTTCACCTTAAGCCAGGAAATGCTGGATATTGTGAACTATCGCAAAGAATCCAATTTATTGTTTGACGTTGGCGTGGCTGATGCACTTTCCAAACGCCTG > minE/17448‑17582 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |