| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 96,401 | T→C | 19.3% | E62E (GAA→GAG) | yacG ← | conserved hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 96,401 | 0 | T | C | 19.3% | 111.2 / 17.6 | 77 | E62E (GAA→GAG) | yacG | conserved hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base T (24/38); new base C (11/4); total (35/42) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.12e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GATGCGACGCTGTCCGCGTCTTATCAGGCCTACGTGCGGCATCAGACA‑AATGTCACTGCTTTGGTTCTTCGCTCCAGTCATCGCTTTCGGAAAGATCGCCACTGCTGGGGATTCGTTTTTCTTCAGCAGCC > minE/96337‑96467 | gATGCGACGCTGTCCGCGTCTTATCAGGCCTACGTGCGGCATCAGACA‑AATGTCACTGCTTTGGttctt < 1:1213536/69‑1 (MQ=255)gATGCGACGCTGTCCGCGTCTTATCAGGCCTACGTGCGGCATCAGACA‑AATGTCACTGCTTTGGttctt < 1:3188855/69‑1 (MQ=255) gCGACGCTGTCCGCGTCTTATCAGGCCTACGTGCGGCATCAGACA‑AATGTCACTGCTTTGGTTCTTCGc < 1:801026/69‑1 (MQ=255) gCGACGCTGTCCGCGTCTTATCAGGCCTACGTGCGGCATCAGACA‑AATGTCACTGCTTTGGTTCTTCGc < 1:1351010/69‑1 (MQ=255) gCGACGCTGTCCGCGTCTTATCAGGCCTACGTGCGGCATCAGACA‑AATGTCACTGCTTTGGTTCTTCGc < 1:2807026/69‑1 (MQ=255) gCGACGCTGTCCGCGTCTTATCAGGCCTACGTGCGGCATCAGACA‑AATGTCACTGCTTTGGTTCTTCGc < 1:571375/69‑1 (MQ=255) gCGACGCTGTCCGCGTCTTATCAGGCCTACGTGCGGCATCAGACA‑AATGTCACTGCTTTGGTTCTTCGc > 1:379922/1‑69 (MQ=255) gCGACGCTGTCCGCGTCTTATCAGGCCTACGTGCGGCATCAGACA‑AATGTCACTGCTTTGGTTCTTCGc < 1:354349/69‑1 (MQ=255) tGTCCGCGTCTTATCAGGCCTACGTGCGGCATCAGACA‑AATGTCACTGCTTTGGTTCTTCGCTCCAGTc < 1:2591389/69‑1 (MQ=255) tGTCCGCGTCTTATCAGGCCTACGTGCGGCATCAGACA‑AATGTCACTGCTTTGGTTCTTCGCTCCAGTc < 1:2164946/69‑1 (MQ=255) gttttATCAGGCCAGCGT‑C‑GCATCAGATAGAATGTCACTGCTTTGGCTCTTCTCTCCAGTCATCGCttt > 1:2708900/4‑69 (MQ=255) gttttATCAGGCCAGCGT‑C‑GCATCAGATAGAATGTCACTGCTTTGGCTCTTCGCTCCAGTCATCGCttt > 1:3331762/4‑69 (MQ=255) gttttATCAGGCCAGCGT‑C‑GCATCAGATAGAATGTCACTGCTTTGGCTCTTCGCTCCAGTCATCGCttt > 1:2765166/4‑69 (MQ=255) gttttATCAGGCCAGCGT‑C‑GCATCAGATAGAATGTCACTGCTTTGGCTCTTCGCTCCAGTCATCGCttt > 1:3122026/4‑69 (MQ=255) gttttATCAGGCCAGCGT‑C‑GCATCAGATAGAATGTCACTGCTTTGGCTCTTCGCTCCAGTCATCGCttt > 1:1779954/4‑69 (MQ=255) gttttATCAGGCCAGCGT‑C‑GCATCAGATAGAATGTCACTGCTTTGGCTCTTCGCTCCAGTCATCGCttt > 1:1718436/4‑69 (MQ=255) gttttATCAGGCCAGCGT‑C‑GCATCAGATAGAATGTCACTGCTTTGGCTCTTCGCTCCAGTCATCGCttt > 1:95025/4‑69 (MQ=255) gttttATCAGGCCAGCGT‑C‑GCATCAGATAGAATGTCACTGCTTTGGCTCTTCGCTCCAGTCATCGCttt > 1:1683262/4‑69 (MQ=255) gttttATCAGGCCAGCGT‑C‑GCATCAGATAGAATGTCACTGCTTTGGCTCTTCGCTCCAGTCATCGCttt > 1:90766/4‑69 (MQ=255) gttttATCAGGCCAGCGT‑C‑GCATCAGATAGAATGTCACTGCTTTGGCTCTTCGCTCCAGTCATCGCttt > 1:690778/4‑69 (MQ=255) gttttATCAGGCCAGCGT‑C‑GCATCAGATAGAATGTCACTGCTTTGGCTCTTCGCTCCAGTCATCGCtt > 1:3186642/4‑68 (MQ=255) ttATCAGGCCTACGTGCGGCATCAGACA‑AATGTCACTGCTTTGGTTCTTCGCTCCAGTCATCGCTTTc > 1:3213804/1‑68 (MQ=255) ttATCAGGCCTACGTGCGGCATCAGACA‑AATGTCACTGCTTTGGTTCTTCGCTCCAGTCATCGCTTTc > 1:1810512/1‑68 (MQ=255) ttATCAGGCCTACGTGCGGCATCAGACA‑AATGTCACTGCTTTGGTTCTTCGCTCCAGTCATCGCTTTc > 1:3021363/1‑68 (MQ=255) ttATCAGGCCTACGTGCGGCATCAGACA‑AATGTCACTGCTTTGGTTCTTCGCTCCAGTCATCGCTTTc > 1:1332660/1‑68 (MQ=255) aGGCCTACGTGCGGCATCAGACA‑AATGTCACTGCTTTGGTTCTTCGCTCCAGTCATCGCTTTCGGAAAg < 1:598360/69‑1 (MQ=255) aGGCCTACGTGCGGCATCAGACA‑AATGTCACTGCTTTGGTTCTTCGCTCCAGTCATCGCTTTCGGAAAg < 1:1381549/69‑1 (MQ=255) aGGCCTACGTGCGGCATCAGACA‑AATGTCACTGCTTTGGTTCTTCGCTCCAGTCATCGCTTTCGGAAAg < 1:2710715/69‑1 (MQ=255) ggCCTACGTGCGGCATCAGACA‑AATGTCACTGCTTTGGTTCTTCGCTCCAGTCATCGCTTTCGGAAAg < 1:1387124/68‑1 (MQ=255) gCCTACGTGCGGCATCAGACA‑AATGTCACTGCTTTGGTTCTTCGCTTCAGTCATCGCTTTCGGAAAGa < 1:2595754/68‑1 (MQ=255) gCCTACGTGCGGCATCAGACA‑AATGTCACTGCTTTGGTTCTTCGCTCCAGTCATCGCTTTCGGAAAGa < 1:953421/68‑1 (MQ=255) gCCTACGTGCGGCATCAGACA‑AATGTCACTGCTTTGGTTCTTCGCTCCAGTCATCGCTTTCGGAAAGa < 1:2533516/68‑1 (MQ=255) gCCTACGTGCGGCATCAGACA‑AATGTCACTGCTTTGGTTCTTCGCTCCAGTCATCGCTTTCGGAAAGa < 1:2865212/68‑1 (MQ=255) gCCTACGTGCGGCATCAGACA‑AATGTCACTGCTTTGGTTCTTCGCTCCAGTCATCGCTTTCGGAAAGa < 1:2849763/68‑1 (MQ=255) gCCTACGTGCGGCATCAGACA‑AATGTCACTGCTTTGGTTCTTCGCTCCAGTCATCGCTTTCGGAAAGa < 1:26114/68‑1 (MQ=255) gCCTACGTGCGGCATCAGACA‑AATGTCACTGCTTTGGTTCTTCGCTCCAGTCATCGCTTTCGGAAAGa < 1:1930476/68‑1 (MQ=255) gCCTACGTGCGGCATCAGACA‑AATGTCACTGCTTTGGTTCTTCGCTCCAGTCATCGCTTTCGGAAAGa < 1:2020797/68‑1 (MQ=255) gCCTACGTGCGGCATCAGACA‑AATGTCACTGCTTTGGTTCTTCGCTCCAGTCATCGCTTTCGGAAAGa < 1:1638629/68‑1 (MQ=255) tACGTGCGGCATCAGACA‑AATGTCACTGCTTTGGTTCTTCGCTCCAGTCATCGCTTTCGGAAAGATCGc < 1:1832667/69‑1 (MQ=255) tACGTGCGGCATCAGACA‑AATGTCACTGCTTTGGTTCTTCGCTCCAGTCATCGCTTTCGGAAAGATCGc < 1:943669/69‑1 (MQ=255) tACGTGCGGCATCAGACA‑AATGTCACTGCTTTGGTTCTTCGCTCCAGTCATCGCTTTCGGAAAGATCGc < 1:1487542/69‑1 (MQ=255) cGTGCGGCATCAGACA‑AATGTCACTGCTTTGGTTCTTCGCTCCAGTCATCGCTTTCGGAAAGATCGc < 1:3068425/67‑1 (MQ=255) cGTGCGGCATCAGACA‑AATGTCACTGCTTTGGTTCTTCGCTCCAGTCATCGCTTTCGGAAAGATCGc < 1:1779710/67‑1 (MQ=255) cGTGCGGCATCAGACA‑AATGTCACTGCTTTGGTTCTTCGCTCCAGTCATCGCTTTCGGAAAGATCGc < 1:1771821/67‑1 (MQ=255) cGTGCGGCATCAGACA‑AATGTCACTGCTTTGGTTCTTCGCTCCAGTCATCGCTTTCGGAAAGATCGc < 1:2775442/67‑1 (MQ=255) cGTGCGGCATCAGACA‑AATGTCACTGCTTTGGTTCTTCGCTCCAGTCATCGCTTTCGGAAAGATCGc < 1:952900/67‑1 (MQ=255) cGTGCGGCATCAGACA‑AATGTCACTGCTTTGGTTCTTCGCTCCAGTCATCGCTTTCGGAAAGATCGc < 1:1227634/67‑1 (MQ=255) cGTGCGGCATCAGACA‑AATGTCACTGCTTTGGTTCTTCGCTCCAGTCATCGCTTTCGGAAAGATCGCCa > 1:3318904/1‑69 (MQ=255) cGTGCGGCATCAGACA‑AATGTCACTGCTTTGGTTCTTCGCTCCAGTCATCGCTTTCGGAAAGATCGCCa > 1:1273635/1‑69 (MQ=255) cGTGCGGCATCAGACA‑AATGTCACTGCTTTGGTTCTTCGCTCCAGTCATCGCTTTCGGAAAGATCGCCa > 1:2377729/1‑69 (MQ=255) cGTGCGGCATCAGACA‑AATGTCACTGCTTTGGTTCTTCGCTCCAGTCATCGCTTTCGGAAAGATCGCCa > 1:1939544/1‑69 (MQ=255) tGCGGCATCAGACA‑AATGTCACTGCTTTGGTTCTTCGCTCCAGTCATCGCTTTCGGAAAGATCGCCAc < 1:1565024/68‑1 (MQ=255) tGCGGCATCAGACA‑AATGTCACTGCTTTGGTTCTTCGCTCCAGTCATCGCTTTCGGAAAGATCGCCAc < 1:1341671/68‑1 (MQ=255) tGCGGCATCAGACA‑AATGTCACTGCTTTGGTTCTTCGCTCCAGTCATCGCTTTCGGAAAGATCGCCAc < 1:958084/68‑1 (MQ=255) tGCGGCATCAGACA‑AATGTCACTGCTTTGGTTCTTCGCTCCAGTCATCGCTTTCGGAAAGATCGCCAc < 1:2029419/68‑1 (MQ=255) aTCAGACA‑AATGTCACTGCTTTGGTTCTTCGCTCCAGTCATCGCTTTCGGAAAGa < 1:1371958/55‑1 (MQ=255) tagaaTGTCACTGCTTTGGCTCTTCGCTCCAGTCATCGCTTTCGGAAAGATCGCTACTGCTGGGGAt < 1:2532670/64‑1 (MQ=255) tagaaTGTCACTGCTTTGGCTCTTCGCTCCAGTCATCGCTTTCGGAAAGATCGCTACTGCTGGGGAt < 1:2527406/64‑1 (MQ=255) tagaaTGTCACTGCTTTGGCTCTTCGCTCCAGTCATCGCTTTCGGAAAGATCGCTACTGCTGGGGAt < 1:606488/64‑1 (MQ=255) cA‑AATGTCACTGCTTTGGTTCTTCGCTCCAGTCATCGCTTTCGGAAAGATCGCCActg > 1:3413677/1‑58 (MQ=255) cA‑AATGTCACTGCTTTGGTTCTTCGCTCCAGTCATCGCTTTCGGAAAGATCGCCACTGCTGGGGATTCg > 1:316700/1‑69 (MQ=255) cA‑AATGTCACTGCTTTGGTTCTTCGCTCCAGTCATCGCTTTCGGAAAGATCGCCACTGCTGGGGATTCg > 1:834692/1‑69 (MQ=255) cA‑AATGTCACTGCTTTGGTTCTTCGCTCCAGTCATCGCTTTCGGAAAGATCGCCACTGCTGGGGATTCg > 1:3040619/1‑69 (MQ=255) cA‑AATGTCACTGCTTTGGTTCTTCGCTCCAGTCATCGCTTTCGGAAAGATCGCCACTGCTGGGGATTCg > 1:3029163/1‑69 (MQ=255) cA‑AATGTCACTGCTTTGGTTCTTCGCTCCAGTCATCGCTTTCGGAAAGATCGCCACTGCTGGGGATTCg > 1:3028254/1‑69 (MQ=255) tCACTGCTTTGGTTCTTCGCTCCAGTCATCGCTTTCGGAAAGATCGCCACTGCTGGGGATTCGTTTttc < 1:3341218/69‑1 (MQ=255) tCACTGCTTTGGTTCTTCGCTCCAGTCATCGCTTTCGGAAAGATCGCCACTGCTGGGGATTCGTTTttc < 1:2132340/69‑1 (MQ=255) cTGCTTTGGCTCTTCGCTCCAGTCATCGCTTTCGGAAAGATCGCTACTGCTGGGGAt < 1:1629906/57‑1 (MQ=255) gCTTTGGTTCTTCGCTCCAGTCATCGCTTTCGGAAAGATCGCCActg > 1:2068440/1‑47 (MQ=255) gCTTTGGTTCTTCGCTCCAGTCATCGCTTTCGGAAAGATCGCCACTGCTGGGGATTCGTTTttcttc > 1:795229/1‑67 (MQ=255) gCTTTGGTTCTTCGCTCCAGTCATCGCTTTCGGAAAGATCGCCACTGCTGGGGATTCGTTTttcttc > 1:2419652/1‑67 (MQ=255) gCTTTGGTTCTTCGCTCCAGTCATCGCTTTCGGAAAGATCGCCACTGCTGGGGATTCGTTTttcttc > 1:1004233/1‑67 (MQ=255) gCTTTGGTTCTTCGCTCCAGTCATCGCTTTCGGAAAGATCGCCACTGCTGGGGATTCGTTTttcttc > 1:1298946/1‑67 (MQ=255) gCTTTGGTTCTTCGCTCCAGTCATCGCTTTCGGAAAGATCGCCACTGCTGGGGATTCGTTTttcttc > 1:2087564/1‑67 (MQ=255) gCTTTGGTTCTTCGCTCCAGTCATCGCTTTCGGAAAGATCGCCACTGCTGGGGATTCGTTTttcttc > 1:499951/1‑67 (MQ=255) gCTTTGGTTCTTCGCTCCAGTCATCGCTTTCGGAAAGATCGCCACTGCTGGGGATTCGTTTttcttc > 1:2072446/1‑67 (MQ=255) gCTTTGGTTCTTCGCTCCAGTCATCGCTTTCGGAAAGATCGCCACTGCTGGGGATTCGTTTttctt > 1:2703215/1‑66 (MQ=255) gTTCTTCGCTCCAGTCATCGCTTTCGGAAAGATCGCCACTGCTGGGGATTCGTTTTTCTTCAGCAGcc < 1:1998053/68‑1 (MQ=255) gTTCTTCGCTCCAGTCATCGCTTTCGGAAAGATCGCCACTGCTGGGGATTCGTTTTTCTTCAGCAGcc < 1:2726020/68‑1 (MQ=255) | GATGCGACGCTGTCCGCGTCTTATCAGGCCTACGTGCGGCATCAGACA‑AATGTCACTGCTTTGGTTCTTCGCTCCAGTCATCGCTTTCGGAAAGATCGCCACTGCTGGGGATTCGTTTTTCTTCAGCAGCC > minE/96337‑96467 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |