| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,161,118 | T→A | 32.6% | F9L (TTT→TTA) | ydjX → | predicted inner membrane protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,161,118 | 0 | T | A | 32.6% | 96.3 / 66.3 | 92 | F9L (TTT→TTA) | ydjX | predicted inner membrane protein |
| Reads supporting (aligned to +/- strand): ref base T (45/17); new base A (17/13); total (62/30) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.57e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.03e-01 | |||||||||||
TCTCTATTATCAAGTTTGATATAGGAAACTCCACGATGAACGCTGAGCGTAAATTTCTTTTTGCCTGTCTTATTTTTGCGCTGGTCATTTACGCTATCCACGCTTTCGGTTTATTCGATCTGCTCACCG > minE/1161057‑1161185 | tctcTATTATCAAGTTTGATATAGGAAACTCCACGATGAACGCTGAGCGTAAATTTCTTTTTGCCTGt > 1:2449263/1‑68 (MQ=255) ctctATTATCAAGTTTGATATAGGAAACTCCACGATGAACGCTGAGCGTAAATTTCTTTTTGCCTGTCt > 1:1915911/1‑69 (MQ=255) tctATTATCAAGTTTGATATAGGAAACTCCACGATGAACGCTGAGCGTAAATTTCTTTTTGCCTGTCtt > 1:1242523/1‑69 (MQ=255) atCAAGTTTGATATAGGAAACTCCACGATGAACGCTGAGCGTAAATTTCTTTTTGCCTGTCTTAttttt > 1:2412459/1‑69 (MQ=255) atCAAGTTTGATATAGGAAACTCCACGATGAACGCTGAGCGTAAATTTCTTTTTGCCTGTCTTAttttt > 1:1986306/1‑69 (MQ=255) atatAGGAAACTCCACGATGAACGCTGAGCGTAAATTTCTTTTTGCCTGTCTTAtt < 1:273107/56‑1 (MQ=255) taGGAAACTCCACGATGAACGCTGAGCGTAAATTTCTTTTTGCCTGTCTTATTTTTGCGCTGGTCAttt > 1:2708399/1‑69 (MQ=255) ggAAACTCCACGATGAACGCTGAGCGTAAATTTCTTTTTGCCTGTCTTATTTTTGCGCTGGTCATTTAc > 1:48701/1‑69 (MQ=255) ggAAACTCCACGATGAACGCTGAGCGTAAATTTCTTTTTGCCTGTCTTATTTTTGCGCTGGTCATTTAc > 1:472436/1‑69 (MQ=255) ggAAACTCCACGATGAACGCTGAGCGTAAATTTCTTTTTGCCTGTCTTATTTTTGCGCTGGTCATTTAc > 1:335586/1‑69 (MQ=255) ggAAACTCCACGATGAACGCTGAGCGTAAATTTCTTTTTGCCTGTCTTATTTTTGCGCTGGTCATTTAc > 1:3320924/1‑69 (MQ=255) ggAAACTCCACGATGAACGCTGAGCGTAAATTTCTTTTTGCCTGTCTTATTTTTGCGCTGGTCATTTAc > 1:3018377/1‑69 (MQ=255) ggAAACTCCACGATGAACGCTGAGCGTAAATTTCTTTTTGCCTGTCTTATTTTTGCGCTGGTCATTTAc > 1:2887410/1‑69 (MQ=255) ggAAACTCCACGATGAACGCTGAGCGTAAATTTCTTTTTGCCTGTCTTATTTTTGCGCTGGTCATTTAc > 1:1060787/1‑69 (MQ=255) ggAAACTCCACGATGAACGCTGAGCGTAAATTTCTTTTTGCCTGTCTTATTTTTGCGCTGGTCATTTAc > 1:274634/1‑69 (MQ=255) ggAAACTCCACGATGAACGCTGAGCGTAAATTTCTTTTTGCCTGTCTTATTTTTGCGCTGGTCATTTAc > 1:2715113/1‑69 (MQ=255) ggAAACTCCACGATGAACGCTGAGCGTAAATTTCTTTTTGCCTGTCTTATTTTTGCGCTGGTCATTTAc > 1:25546/1‑69 (MQ=255) ggAAACTCCACGATGAACGCTGAGCGTAAATTTCTTTTTGCCTGTCTTATTTTTGCGCTGGTCATTTAc < 1:664197/69‑1 (MQ=255) ggAAACTCCACGATGAACGCTGAGCGTAAATTTCTTTTTGCCTGTCTTATTTTTGCGCTGGTCATTTAc < 1:966113/69‑1 (MQ=255) ggAAACTCCACGATGAACGCTGAGCGTAAATTTCTTTTTGCCTGTCTTATTTTTGCGCTGGTCATTTAc > 1:124095/1‑69 (MQ=255) ggAAACTCCACGATGAACGCTGAGCGTAAATTTCTTTTTGCCTGTCTTATTTTTGCGCTGGTCATTTAc > 1:1089781/1‑69 (MQ=255) ggAAACTCCACGATGAACGCTGAGCGTAAATTTCTTTTTGCCTGTCTTATTTTTGCGCTGGTCATTTAc > 1:168634/1‑69 (MQ=255) ggAAACTCCACGATGAACGCTGAGCGTAAATTTCTTTTTGCCTGTCTTATTTTTGAGCTGGTCATTTAc > 1:184672/1‑69 (MQ=255) ggAAACTCCACGATGAACGCTGAGCGTAAATTTCTTTTTGCCTGTCTTATTTTTGAGCTGGTCATTTAc > 1:540831/1‑69 (MQ=255) ggAAACTCCACGATGAACGCTGAGCGTAAATTTCTTTTAGCCTg > 1:1463154/1‑44 (MQ=255) ggAAACTCCACGATGAACGCTGAGCGTAAATTTCTTTTAGCCTg > 1:3175344/1‑44 (MQ=255) ggAAACTCCACGATGAACGCTGAGCGTAAATTTCTTTTAGCCTg > 1:1501682/1‑44 (MQ=255) ggAAACTCCACGATGAACGCTGAGCGTAAATTTCTTTTAGCCTg > 1:328901/1‑44 (MQ=255) ggAAACTCCACGATGAACGCTGAGCGTAAATTTCTTTTAGCCTg > 1:2905108/1‑44 (MQ=255) ggAAACTCCACGATGAACGCTGAGCGTAAATTTCTTTTAGCCTg > 1:1129738/1‑44 (MQ=255) ggAAACTCCACGATGAACGCTGAGCGTAAATTTCTTTTAGCCTg > 1:1599755/1‑44 (MQ=255) ggAAACTCCACGATGAACGCTGAGCGTAAATTTCTTTTAGCCTg > 1:104645/1‑44 (MQ=255) ggAAACTCCACGATGAACGCTGAGCGTAAATTTCTTTTAGCCTg > 1:2270116/1‑44 (MQ=255) ggAAACTCCACGATGAACGCTGAGCGTAAATTTCTTTTAGCCTg > 1:1066908/1‑44 (MQ=255) ggAAACTCCACGATGAACGCTGAGCGTAAATTTCTTTTAGCCTg > 1:2237452/1‑44 (MQ=255) ggAAACTCCACGATGAACGCTGAGCGTAAATTTCTTTTAGCCTg > 1:2233332/1‑44 (MQ=255) ggAAACTCCACGATGAACGCTGAGCGTAAATTTCTTTTAGCCTg > 1:2201202/1‑44 (MQ=255) ggAAACTCCACGATGAACGCTGAGCGTAAATTTCTTTTAGCCTg > 1:2100214/1‑44 (MQ=255) ggAAACTCCACGATGAACGCTGAGCGTAAATTTCTTTTAGCCTg > 1:762997/1‑44 (MQ=255) ggAAACTCCACGATGAACGCTGAGCGTAAATTTCTTTTAGCCTg > 1:932982/1‑44 (MQ=255) ggAAACTCCACGATGAACGCTGAGCGTAAATTTCTTTTAGCCTg > 1:2205805/1‑44 (MQ=255) ggAAACTCAACGATGAACGCTGAGCGTAAATTTCTTTTTGCCTGTCTTATTTTTGCGCTGGTCATTTAc > 1:2758774/1‑69 (MQ=255) aaCTCCACGATGAACGCTTAGCGTAAATTTCTTTTTGCCTGTCTTATTTTTGCGCTGGTCATTTACGCt > 1:3127862/1‑69 (MQ=255) aaCTCCACGATGAACGCTGAGCGTAAATTTCTTTTTGCCTGTCTTATTTTTGCGCTGGTCATTTACGCt > 1:168753/1‑69 (MQ=255) aaCTCCACGATGAACGCTGAGCGTAAATTTCTTTTTGCCTGTCTTATTTTTGCGCTGGTCATTTACGCt < 1:2751974/69‑1 (MQ=255) aaCTCCACGATGAACGCTGAGCGTAAATTTCTTTTTGCCTGTCTTATTTTTGCGCTGGTCATTTACGCt < 1:2895504/69‑1 (MQ=255) aaCTCCACGATGAACGCTGAGCGTAAATTTCTTTTTGCCTGTCTTATTTTTGCGCTGGTCATTTACGCt < 1:950502/69‑1 (MQ=255) aaCTCCACGATGAACGCTGAGCGTAAATTTCTTTTTGCCTGTCTTATTTTTGCGCTGGTCATTTACGCt > 1:2046432/1‑69 (MQ=255) aaCTCCACGATGAACGCTGAGCGTAAATTTCTTTTTGCCTGTCTTATTTTTGCGCTGGTCATTTACGCt < 1:573028/69‑1 (MQ=255) aCTCCACGATGAACGCTGAGCGTAAATTTCTTTTTGCCTGTCTTATTTTTGCGCTGGTCATTTACGCt < 1:1349143/68‑1 (MQ=255) tCCACGATGAACGCTGAGCGTAAATTTCTTTTTGCCTGTCTTATTTTTGCGCTGGTCATTTACGCTATc < 1:1169184/69‑1 (MQ=255) tCCACGATGAACGCTGAGCGTAAATTTCTTTTTGCCTGTCTTATTTTTGCGCTGGTCATTTACGCTATc < 1:2842301/69‑1 (MQ=255) cACGATGAACGCTGAGCGTAAATTTCTTTTTGCCTGTCtt > 1:676876/1‑40 (MQ=255) cACGATGAACGCTGAGCGTAAATTTCTTTTTGCCTGTCtt > 1:1812206/1‑40 (MQ=255) cACGATGAACGCTGAGCGTAAATTTCTTTTTGCCTGTCtt > 1:401452/1‑40 (MQ=255) aCGATGAACGCTGAGCGTAAATTTCTTTTTGCCTGTCTTATTTTTGCGCTGGTCATTTACGCTATCCAc > 1:583334/1‑69 (MQ=255) aCGATGAACGCTGAGCGTAAATTTCTTTTTGCCTGTCTTATTTTTGCGCTGGTCATTTACGCTATCCAc > 1:2818271/1‑69 (MQ=255) aCGATGAACGCTGAGCGTAAATTTCTTTTTGCCTGTCTTATTTTTGCGCTGGTCATTTACGCTATCCAc > 1:1395017/1‑69 (MQ=255) aCGATGAACGCTGAGCGTAAATTTCTTTTTGCCTGTCTTATTTTTGCGCTGGTCATTTACGCTATCCAc > 1:2523707/1‑69 (MQ=255) aCGATGAACGCTGAGCGTAAATTTCTTTTTGCATGTCTTATTTTTGCGCTGGTCATTTACGCTATCCAc > 1:1973828/1‑69 (MQ=255) tGAGCGTAAATTTCTTTTAGCCTGTCTTATTTTTGCGCTGGTCATTTACGCTATCCACGCTTTCGGttt < 1:357999/69‑1 (MQ=255) tGAGCGTAAATTTCTTTTAGCCTGTCTTATTTTTGCGCTGGTCATTTACGCTATCCACGCTTTCGGttt < 1:180679/69‑1 (MQ=255) tGAGCGTAAATTTCTTTTAGCCTGTCTTATTTTTGCGCTGGTCATTTACGCTATCCACGCTTTCGGttt < 1:1922887/69‑1 (MQ=255) tGAGCGTAAATTTCTTTTAGCCTGTCTTATTTTTGCGCTGGTCATTTACGCTATCCACGCTTTCGGttt < 1:106226/69‑1 (MQ=255) tGAGCGTAAATTTCTTTTAGCCTGTCTTATTTTTGCGCTGGTCATTTACGCTATCCACGCTTTCGGttt < 1:2253034/69‑1 (MQ=255) tGAGCGTAAATTTCTTTTAGCCTGTCTTATTTTTGCGCTGGTCATTTACGCTATCCACGCTTTCGGttt < 1:452116/69‑1 (MQ=255) tGAGCGTAAATTTCTTTTAGCCTGTCTTATTTTTGCGCTGGTCATTTACGCTATCCACGCTTTCGGttt < 1:2401025/69‑1 (MQ=255) tGAGCGTAAATTTCTTTTAGCCTGTCTTATTTTTGCGCTGGTCATTTACGCTATCCACGCTTTCGGttt < 1:1132674/69‑1 (MQ=255) tGAGCGTAAATTTCTTTTAGCCTGTCTTATTTTTGCGCTGGTCATTTACGCTATCCACGCTTTCGGttt < 1:2810670/69‑1 (MQ=255) tGAGCGTAAATTTCTTTTAGCCTGTCTTATTTTTGCGCTGGTCATTTACGCTATCCACGCTTTCGGttt < 1:2647202/69‑1 (MQ=255) tGAGCGTAAATTTCTTTTAGCCTGTCTTATTTTTGCGCTGGTCATTTACGCTATCCACGCTTTCGGttt < 1:1251212/69‑1 (MQ=255) tGAGCGTAAATTTCTTTTAGCCTGTCTTATTTTTGCGCTGGTCATTTACGCTATCCACGCTTTCGGttt < 1:3034462/69‑1 (MQ=255) tGAGCGTAAATTTCTTTTAGCCTGTCTTATTTTTGCGCTGGTCATTTACGCTATCCACGCTTTCGGttt < 1:3258220/69‑1 (MQ=255) tAAATTTCTTTTTGCCTGTCTTATTTTTGCGCTGGTCATTTACGCTATCCACGCTTTCGGTTTATTCGa > 1:2174929/1‑69 (MQ=255) tAAATTTCTTTTTGCCTGTCTTATTTTTGCGCTGGTCATTTACGCTATCCACGCTTTCGGTTTATTCGa < 1:1909243/69‑1 (MQ=255) tAAATTTCTTTTTGCCTGTCTTATTTTTGCGCTGGTCATTTACGCTATCCACGCTTTCGGTTTATTCGa > 1:814096/1‑69 (MQ=255) tAAATTTCTTTTTGCCTGTCTTATTTTTGCGCTGGTCATTTACGCTATCCACGCTTTCGGTTTATTCGa > 1:1258230/1‑69 (MQ=255) tAAATTTCTTTTTGCCTGTCTTATTTTTGCGCTGGTCATTTACGCTATCCACGCTTTCGGTTTATTCGa > 1:741791/1‑69 (MQ=255) tAAATTTCTTTTTGCCTGTCTTATTTTTGCGCTGGTCATTTACGCTATCCACGCTTTCGGTTTATTCGa > 1:717501/1‑69 (MQ=255) tAAATTTCTTTTTGCCTGTCTTATTTTTGCGCTGGTCATTTACGCTATCCACGCTTTCGGTTTATTCGa < 1:2003694/69‑1 (MQ=255) tAAATTTCTTTTTGCCTGTCTTATTTTTGCGCTGGTCATTTACGCTATCCACGCTTTCGGTTTATTCGa < 1:2911451/69‑1 (MQ=255) tAAATTTCTTTTTGCCTGTCTTATTTTTGCGCTGGTCATTTACGCTATCCACGCTTTCGGTTTATTCGa > 1:2079243/1‑69 (MQ=255) tAAATTTCTTTTTGCCTGTCTTATTTTTGCGCTGGTCATTTACGCTATCCACGCTTTCGGTTTATTCGa < 1:2155346/69‑1 (MQ=255) tAAATTTCTTTTTGCCTGTCTTATTTTTGCGCTGGTCATTTACGCTATCCACGCTTTCGGTTTATTCGa < 1:29927/69‑1 (MQ=255) tAAATTTCTTTTTGCCTGTCTTATTTTTGCGCTGGTCATTTACGCTATCCACGCTTTCGGTTTATTCGa > 1:2285638/1‑69 (MQ=255) tAAATTTCTTTTTGCCTGTCTTATTTTTGCGCTGGTCATTTACGCTATCCACGCTTTCGGTTTATTCGa > 1:3028527/1‑69 (MQ=255) tAAATTTCTTTTTGCCTGTCTTATTTTTGCGCTGGTCATTTACGCTATCCACGCTTTCGGTTTATTCGa > 1:348049/1‑69 (MQ=255) tAAATTTCTTTTTGCCTGTCTTATTTTTGCGCTGGTCATTTACGCTATCCACGCTTTCGGTTTATTCGa > 1:3387892/1‑69 (MQ=255) tAAATTTCTTTTTGCCTGTCTTATTTTTGCGCTGGTCATTTACGCTATCCACGCTTTCGGTTTATTCGa > 1:3368573/1‑69 (MQ=255) tAAATTTCTTTTTGCCTGTCTTATTTTTGCGCTGGTCATTTACGCTATCCACGCTTTCGGTTTATTCGa < 1:2431867/69‑1 (MQ=255) tAAATTTCTTTTTGCCTGTCTTATTTTTGCGCTGGTCATTTACGCTATCCACGCTTACGGTTTATTCGa > 1:826348/1‑69 (MQ=255) aaTTTCTTTTTGCCTGTCTTATTTTTGCGCTGGTCATTTACGCTATCCACGCTTTCGGTTTATTCGa < 1:1698493/67‑1 (MQ=255) tGCCTGTCTTATTTTTGCGCTGGTCATTTACGCTATCCACGCTTTCGGTTTATTCGATCTGCTCACCg < 1:2442370/68‑1 (MQ=255) | TCTCTATTATCAAGTTTGATATAGGAAACTCCACGATGAACGCTGAGCGTAAATTTCTTTTTGCCTGTCTTATTTTTGCGCTGGTCATTTACGCTATCCACGCTTTCGGTTTATTCGATCTGCTCACCG > minE/1161057‑1161185 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |