| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,162,506 | T→A | 48.8% | M3K (ATG→AAG) | ydjZ → | conserved inner membrane protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,162,506 | 0 | T | A | 48.8% | ‑0.5 / 102.5 | 84 | M3K (ATG→AAG) | ydjZ | conserved inner membrane protein |
| Reads supporting (aligned to +/- strand): ref base T (7/36); new base A (21/20); total (28/56) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.04e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.13e-01 | |||||||||||
Only 100 of 104 total aligned reads displayed.
GGCGGATAACACGCTGGCAACGGTTACCTTTAAAATCGCCGAATAAAGCCAGGATAAAGGATGATGATGATGCAATCGCGAAAAATCTGGTACTACCGTATAACCCTCATCATCCTGTTGTTCGCTAT > minE/1162439‑1162566 | ggcggATAACACGCTGGCAACGGTTACCTTTAAAATCGCCGAATAAAGCCAGGATAAAGgatgatgatg > 1:1613017/1‑69 (MQ=255)ggcggATAACACGCTGGCAACGGTTACCTTTAAAATCGCCGAATAAAGCCAGGATAAAGgatgatgat < 1:1595321/68‑1 (MQ=255) gATAACACGCTGGCAACGGTTACCTTTAAAATCGCCGAATAAAGCCAGGATAAAGGATGATGATGATGc < 1:2773632/69‑1 (MQ=255) gATAACACGCTGGCAACGGTTACCTTTAAAATCGCCGAATAAAGCCAGGATAAAGGATGATGATGATGc < 1:3233280/69‑1 (MQ=255) gATAACACGCTGGCAACGGTTACCTTTAAAATCGCCGAATAAAGCCAGGATAAAGGATGATGATGATGc < 1:696847/69‑1 (MQ=255) tAACACGCTGGCAACGGTTACCTTTAAAATCGCCGAATAAAGCCAGGATAAAGGATGATGATGATGCaa > 1:3085594/1‑69 (MQ=255) acacGCTGGCAACGGTTACCTTTAAAATCGCCGAATAAAGCCAGGATAAAGGATGATGATGATGCAATc > 1:1651856/1‑69 (MQ=255) acGCTGGCAACGGTTACCTTTAAAATCGCCGAATAAAGCCAGGATAAAGGATGATGATGATGCAATcg > 1:2785206/1‑68 (MQ=255) acGCTGGCAACGGTTACCTTTAAAATCGCCGAATAAAGCCAGGATAAAGGATGATGATGATGCAATcg > 1:2945717/1‑68 (MQ=255) acGCTGGCAACGGTTACCTTTAAAATCGCCGAATAAAGCCAGGATAAAGGATGATGATGATGCAATcg > 1:1741778/1‑68 (MQ=255) cTGGCAACGGTTACCTTTAAAATCACCGAATAAAG‑CAGGATAACGGATGATGAAGATGCAATCGCGtaa < 1:2112329/69‑1 (MQ=255) cTGGCAACGGTTACCTTTAAAATCACCGAATAAAG‑CAGGATAACGGATGATGAAGATGCAATCGCGtaa < 1:2007976/69‑1 (MQ=255) cTGGCAACGGTTACCTTTAAAATCACCGAATAAAG‑CAGGATAACGGATGATGAAGATGCAATCGCGtaa < 1:1868852/69‑1 (MQ=255) cTGGCAACGGTTACCTTTAAAATCACCGAATAAAG‑CAGGATAACGGATGATGAAGATGCAATCGCGtaa < 1:317715/69‑1 (MQ=255) cTGGCAACGGTTACCTTTAAAATCACCGAATAAAG‑CAGGATAACGGATGATGAAGATGCAATCGCGtaa < 1:403217/69‑1 (MQ=255) cTGGCAACGGTTACCTTTAAAATCACCGAATAAAG‑CAGGATAACGGATGATGAAGATGCAATCGCGtaa < 1:428076/69‑1 (MQ=255) cTGGCAACGGTTACCTTTAAAATCACCGAATAAAG‑CAGGATAACGGATGATGAAGATGCAATCGCGtaa < 1:1441706/69‑1 (MQ=255) cTGGCAACGGTTACCTTTAAAATCACCGAATAAAG‑CAGGATAACGGATGATGAAGATGCAATCGCGtaa < 1:2223879/69‑1 (MQ=255) cTGGCAACGGTTACCTTTAAAATCACCGAATAAAG‑CAGGATAACGGATGATGAAGATGCAATCGCGtaa < 1:1382048/69‑1 (MQ=255) cTGGCAACGGTTACCTTTAAAATCACCGAATAAAG‑CAGGATAACGGATGATGAAGATGCAATCGCGtaa < 1:604920/69‑1 (MQ=255) cTGGCAACGGTTACCTTTAAAATCACCGAATAAAG‑CAGGATAACGGATGATGAAGATGCAATCGCGtaa < 1:694397/69‑1 (MQ=255) cTGGCAACGGTTACCTTTAAAATCACCGAATAAAG‑CAGGATAACGGATGATGAAGATGCAATCGCGtaa < 1:2232104/69‑1 (MQ=255) cTGGCAACGGTTACCTTTAAAATCACCGAATAAAG‑CAGGATAACGGATGATGAAGATGCAATCGCGtaa < 1:83977/69‑1 (MQ=255) cTGGCAACGGTTACCTTTAAAATCACCGAATAAAG‑CAGGATAACGGATGATGAAGATGCAATCGCGtaa < 1:1161609/69‑1 (MQ=255) cTGGCAACGGTTACCTTTAAAATCACCGAATAAAG‑CAGGATAACGGATGATGAAGATGCAATCGCGtaa < 1:1197583/69‑1 (MQ=255) cTGGCAACGGTTACCTTTAAAATCACCGAATAAAG‑CAGGATAACGGATGATGAAGATGCAATCGCGtaa < 1:1187059/69‑1 (MQ=255) cTGGCAACGGTTACCTTTAAAATCACCGAATAAAG‑CAGGATAACGGATGATGAAGATGCAATCGCGtaa < 1:1176286/69‑1 (MQ=255) cTGGCAACGGTTACCTTTAAAATCACCGAATAAAG‑CAGGATAACGGATGATGAAGATGCAATCGCGtaa < 1:774555/69‑1 (MQ=255) tGGCAACGGTTACCTTTAAAATCACCGAATAAAG‑CAGGATAACGGATGATGAAGATGCAATCGCGtaa < 1:3255707/68‑1 (MQ=255) aCGGTTACCTTTAAAATCGCCGAATAAAGCCAGGATAAAGgatgatgat < 1:3226587/49‑1 (MQ=255) aCGGTTACCTTTAAAATCGCCGAATAAAGCCAGGATAAAGgatgatgat < 1:2086135/49‑1 (MQ=255) aCGGTTACCTTTAAAATCGCCGAATAAAGCCAGGATAAAGgatgatgat < 1:2140909/49‑1 (MQ=255) aCGGTTACCTTTAAAATCGCCGAATAAAGCCAGGATAAAGgatgatgat < 1:313435/49‑1 (MQ=255) aCGGTTACCTTTAAAATCGCCGAATAAAGCCAGGATAAAGgatgatgat < 1:1523825/49‑1 (MQ=255) aCGGTTACCTTTAAAATCGCCGAATAAAGCCAGGATAAAGgatgatgat < 1:1246881/49‑1 (MQ=255) aCGGTTACCTTTAAAATCGCCGAATAAAGCCAGGATAAAGgatgatgat < 1:1253709/49‑1 (MQ=255) aCGGTTACCTTTAAAATCGCCGAATAAAGCCAGGATAAAGgatgatgat < 1:2756421/49‑1 (MQ=255) ggTTACCTTTAAAATCGCCGAATAAAGCCAGGATAAAGGATGATGATGATGCAATCGCGAAAAATCTgg < 1:2420909/69‑1 (MQ=255) ggTTACCTTTAAAATCGCCGAATAAAGCCAGGATAAAGGATGATGATGATGCAATCGCGAAAAATCTgg < 1:39483/69‑1 (MQ=255) ggTTACCTTTAAAATCGCCGAATAAAGCCAGGATAAAGGATGATGATGATGCAATCGCGAAAAATCTgg < 1:2791726/69‑1 (MQ=255) ggTTACCTTTAAAATCGCCGAATAAAGCCAGGATAAAGGATGATGATGATGCAATCGCGAAAAATCTgg < 1:37019/69‑1 (MQ=255) ggTTACCTTTAAAATCGCCGAATAAAGCCAGGATAAAGGATGATGATGATGCAATCGCGAAAAATCTgg < 1:24380/69‑1 (MQ=255) ggTTACCTTTAAAATCGCCGAATAAAGCCAGGATAAAGGATGATGATGATGCAATCGCGAAAAATCTgg < 1:521681/69‑1 (MQ=255) ggTTACCTTTAAAATCGCCGAATAAAGCCAGGATAAAGGATGATGATGATGCAATCGCGAAAAATCTgg < 1:920617/69‑1 (MQ=255) ggTTACCTTTAAAATCGCCGAATAAAGCCAGGATAAAGGATGATGATGATGCAATCGCGAAAAATCTgg < 1:1702353/69‑1 (MQ=255) ggTTACCTTTAAAATCGCCGAATAAAGCCAGGATAAAGGATGATGATGATGCAATCGCGAAAAATCTgg < 1:2196785/69‑1 (MQ=255) ggTTACCTTTAAAATCGCCGAATAAAGCCAGGATAAAGGATGATGATGATGCAATCGCGAAAAATCTgg < 1:1870603/69‑1 (MQ=255) ggTTACCTTTAAAATCGCCGAATAAAGCCAGGATAAAGGATGATGATGATGCAATCGCGAAAAATCTgg < 1:1873194/69‑1 (MQ=255) ggTTACCTTTAAAATCGCCGAATAAAGCCAGGATAAAGGATGATGATGATGCAATCGCGAAAAATCTgg < 1:1987008/69‑1 (MQ=255) ggTTACCTTTAAAATCGCCGAATAAAGCCAGGATAAAGGATGATGATGATGCAATCGCGAAAAATCTgg < 1:1333755/69‑1 (MQ=255) ggTTACCTTTAAAATCGCCGAATAAAGCCAGGATAAAGGATGATGATGATGCAATCGCGAAAAATCTgg < 1:1344828/69‑1 (MQ=255) tACCTTTAAAATCGCCGAATAAAGCCAGGATAAAGGATGATGATGATGCAATCGCGAAAAATCTGGtac > 1:2309605/1‑69 (MQ=255) tACCTTTAAAATCGCCGAATAAAGCCAGGATAAAGGATGATGATGATGCAATCGCGAAAAATCTGGtac > 1:785219/1‑69 (MQ=255) tttAAAATCGCCGAATAAAGTCAGGATAAAGGATGATGATGATGCAATCGCGAAAAATCTGTtactac < 1:2450103/68‑1 (MQ=255) tttAAAATCGCCGAATAAAGCCAGGATAAAGGATGATGATGATGCAATCGCGAAAAATCTGGtactac < 1:575851/68‑1 (MQ=255) tttAAAATCGCCGAATAAAGCCAGGATAAAGGATGATGATGATGCAATCGCGAAAAATCTGGtactac < 1:1847434/68‑1 (MQ=255) tttAAAATCGCCGAATAAAGCCAGGATAAAGGATGATGATGATGCAATCGCGAAAAATCTGGtactac < 1:3098052/68‑1 (MQ=255) tttAAAATCGCCGAATAAAGCCAGGATAAAGGATGATGATGATGCAATCGCGAAAAATCTGGtactac < 1:1689982/68‑1 (MQ=255) tttAAAATCGCCGAATAAAGCCAGGATAAAGGATGATGATGATGCAATCGCGAAAAATCTGGtactac < 1:452173/68‑1 (MQ=255) tttAAAATCGCCGAATAAAGCCAGGATAAAGGATGATGATGATGCAATCGCGAAAAATCTGGtactac < 1:2451487/68‑1 (MQ=255) tttAAAATCGCCGAATAAAGCCAGGATAAAGGATGATGATGATGCAATCGCGAAAAATCTGGtactac < 1:79215/68‑1 (MQ=255) tttAAAATCGCCGAATAAAGCCAGGATAAAGGATGATGATGATGCAATCGCGAAAAATCTGGtactac < 1:1152982/68‑1 (MQ=255) tttAAAATCGCCGAATAAAGCCAGGATAAAGGATGATGATGATGCAATCGCGAAAAATCTGGtactac < 1:2288312/68‑1 (MQ=255) tttAAAATCGCCGAATAAAGCCAGGATAAAGGATGATGATGATGCAATCGCGAAAAATCTGGtactac < 1:2375014/68‑1 (MQ=255) tAAAG‑CAGGATAACGGATGATGAAGATGCAATCGCGtaa < 1:591162/39‑1 (MQ=16) acggATGATGAAGATGCAATCGCGTAAAATCTGGtactac > 1:2004225/3‑40 (MQ=37) acggATGATGAAGATGCAATCGCGTAAAATCTGGTACTACCGTATaa > 1:1656630/3‑47 (MQ=38) acggATGATGAAGATGCAATCGCGTAAAATCTGGTACTACCGTATAAccc > 1:1185324/3‑50 (MQ=255) acggATGATGAAGATGCAATCGCGTAAAATCTGGTACTACCGTATAACCCt > 1:115265/3‑51 (MQ=255) acggATGATGAAGATGCAATCGCGTAAAATCTGGTACTACCGTATAACCCt > 1:142379/3‑51 (MQ=255) acggATGATGAAGATGCAATCGCGTAAAATCTGGTACTACCGTATAACCCt > 1:587222/3‑51 (MQ=255) acggATGATGAAGATGCAATCGCGTAAAATCTGGTACTACCGTATAACCCt > 1:3300012/3‑51 (MQ=255) acggATGATGAAGATGCAATCGCGTAAAATCTGGTACTACCGTATAACCCt > 1:3299631/3‑51 (MQ=255) acggATGATGAAGATGCAATCGCGTAAAATCTGGTACTACCGTATAACCCt > 1:1713891/3‑51 (MQ=255) acggATGATGAAGATGCAATCGCGTAAAATCTGGTACTACCGTATAACCCt > 1:3199882/3‑51 (MQ=255) acggATGATGAAGATGCAATCGCGTAAAATCTGGTACTACCGTATAACCCt > 1:2419846/3‑51 (MQ=255) acggATGATGAAGATGCAATCGCGTAAAATCTGGTACTACCGTATAACCCt > 1:2422342/3‑51 (MQ=255) acggATGATGAAGATGCAATCGCGTAAAATCTGGTACTACCGTATAACCCt > 1:1097678/3‑51 (MQ=255) acggATGATGAAGATGCAATCGCGTAAAATCTGGTACTACCGTATAACCCt > 1:2472114/3‑51 (MQ=255) acggATGATGAAGATGCAATCGCGTAAAATCTGGTACTACCGTATAACCCt > 1:2615925/3‑51 (MQ=255) acggATGATGAAGATGCAATCGCGTAAAATCTGGTACTACCGTATAACCCt > 1:2711591/3‑51 (MQ=255) acggATGATGAAGATGCAATCGCGTAAAATCTGGTACTACCGTATAACCCt > 1:2189444/3‑51 (MQ=255) acggATGATGAAGATGCAATCGCGTAAAATCTGGTACTACCGTATAACCCt > 1:2052896/3‑51 (MQ=255) acggATGATGAAGATGCAATCGCGTAAAATCTGGTACTACCGTATAACCCt > 1:1762789/3‑51 (MQ=255) acggATGATGAAGATGCAATCGCGTAAAATCTGGTACTACCGTATAACCCt > 1:3157149/3‑51 (MQ=255) acggATGATGAAGATGCAATCGCGTAAAATCTGGTACTACCGTATAACCCt > 1:306804/3‑51 (MQ=255) aaGGATGATGATGATGCAATCGCGAAAAATCTGGTACTACCGTATAACCCTCATCATCCTGTTGTTCGc < 1:1649112/69‑1 (MQ=255) ggATGATGATGATGCAATCGCGAAAAATCTGGTACTACCGTATAACCCTCATCATCCTGTTGTTCGCTa < 1:2799209/69‑1 (MQ=255) ggATGATGATGATGCAATCGCGAAAAATCTGGTACTACCGTATAACCCTCATCATCCTGTTGTTCGCTa < 1:244892/69‑1 (MQ=255) ggATGATGATGATGCAATCGCGAAAAATCTGGTACTACCGTATAACCCTCATCATCCTGTTGTTCGCTa < 1:1573856/69‑1 (MQ=255) ggATGATGATGATGCAATCGCGAAAAATCTGGTACTACCGTATAACCCTCATCATCCTGTTGTTCGCTa < 1:1302499/69‑1 (MQ=255) gatgatgatgatGCAATCGCGAAAAATCTGGTACTACCGTATAACCCTCATCATCCTGTTGTTCGCTAt < 1:293315/69‑1 (MQ=255) gatgatgatgatGCAATCGCGAAAAATCTGGTACTACCGTATAACCCTCATCATCCTGTTGTTCGCTAt < 1:3299587/69‑1 (MQ=255) gatgatgatgatGCAATCGCGAAAAATCTGGTACTACCGTATAACCCTCATCATCCTGTTGTTCGCTAt < 1:345593/69‑1 (MQ=255) gatgatgatgatGCAATCGCGAAAAATCTGGTACTACCGTATAACCCTCATCATCCTGTTGTTCGCTAt < 1:2881639/69‑1 (MQ=255) gatgatgatgatGCAATCGCGAAAAATCTGGTACTACCGTATAACCCTCATCATCCTGTTGTTCGCTAt < 1:2107526/69‑1 (MQ=255) gatgatgatgatGCAATCGCGAAAAATCTGGTACTACCGTATAACCCTCATCATCCTGTTGTTCGCTAt < 1:531710/69‑1 (MQ=255) gatgatgatgatGCAATCGCGAAAAATCTGGTACTACCGTATAACCCTCATCATCCTGTTGTTCGCTAt < 1:473793/69‑1 (MQ=255) gatgatgatgatGCAATCGCGAAAAATCTGGTACTACCGTATAACCCTCATCATCCTGTTGTTCGCTAt < 1:2804211/69‑1 (MQ=255) atgatgatgatgCAATCGCGAAAAATCTGGTACTACCGTATAACCCTCATCATCCTGTTGTTCGc < 1:789301/65‑1 (MQ=255) | GGCGGATAACACGCTGGCAACGGTTACCTTTAAAATCGCCGAATAAAGCCAGGATAAAGGATGATGATGATGCAATCGCGAAAAATCTGGTACTACCGTATAACCCTCATCATCCTGTTGTTCGCTAT > minE/1162439‑1162566 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |