| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,162,897 | G→A | 24.2% | V133V (GTG→GTA) | ydjZ → | conserved inner membrane protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,162,897 | 0 | G | A | 24.2% | 44.1 / 8.9 | 37 | V133V (GTG→GTA) | ydjZ | conserved inner membrane protein |
| Reads supporting (aligned to +/- strand): ref base G (11/17); new base A (3/6); total (14/23) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CTTTTTTATCGCCAGAGTGATGGGCCGCGAAGTGGTGGAAAAATTAACCGGCAAAACCGTGCTTGACAGTATGGACGGCTTTTTCACTCGCTACGGCAAACACACCATTCTGGTCTGTCGGT > minE/1162837‑1162958 | cTTTTTTATCGCCAGAGTGATGGGCCGCGAAGTGGTGGAAAAATTAACCGGCAAAACCGTGCTTGACAg > 1:1475160/1‑69 (MQ=255) ttATCGCCAGAGTGATGGGCCGCGAAGTGGTGGAAAAATTAACCGGCAAAACCGTACTTGACAGTAtt < 1:3295404/68‑2 (MQ=255) ttATCGCCAGAGTGATGGGCCGCGAAGTGGTGGAAAAATTAACCGGCAAAACCGTACTTGACAGTATg < 1:1218658/68‑1 (MQ=255) ttATCGCCAGAGTGATGGGCCGCGAAGTGGTGGAAAAATTAACCGGCAAAACCGTACTTGACAGTATg < 1:2662806/68‑1 (MQ=255) ttATCGCCAGAGTGATGGGCCGCGAAGTGGTGGAAAAATTAACCGGCAAAACCGTACTTGACAGTATg < 1:169847/68‑1 (MQ=255) ttATCGCCAGAGTGATGGGCCGCGAAGTGGTGGAAAAATTAACCGGCAAAACCGTACTTGACAGTATg < 1:1509387/68‑1 (MQ=255) ttATCGCCAGAGTGATGGGCCGCGAAGTGGTGGAAAAATTAACCGGCAAAACCGTACTTGACAGTATg < 1:371712/68‑1 (MQ=255) gTGATGTGCCGCGAAGTGGTGGAAAAATTAACC‑GCAAAACCGTGCTTGACAGTATGGACGGCttttt < 1:1652832/67‑1 (MQ=255) gTGATGGGCCGCGAAGTGGTGGAAAAATTAACCGGCAAAACCGTGCTTGACAGTa > 1:201751/1‑55 (MQ=255) gTGATGGGCCGCGAAGTGGTGGAAAAATTAACCGGCAAAACCGTGCTTGACAGTATg > 1:1492527/1‑57 (MQ=255) gTGATGGGCCGCGAAGTGGTGGAAAAATTAACCGGCAAAACCGTGCTTGACAGTATGGACGGCttttt < 1:3336720/68‑1 (MQ=255) gTGATGGGCCGCGAAGTGGTGGAAAAATTAACCGGCAAAACCGTGCTTGACAGTATGGACGGCttttt < 1:560335/68‑1 (MQ=255) gTGATGGGCCGCGAAGTGGTGGAAAAATTAACCGGCAAAACCGTGCTTGACAGTATGGACGGCttttt < 1:755047/68‑1 (MQ=255) gTGATGGGCCGCGAAGTGGTGGAAAAATTAACCGGCAAAACCGTGCTTGACAGTATGGACGGCttttt < 1:931122/68‑1 (MQ=255) gTGATGGGCCGCGAAGTGGTGGAAAAATTAACCGGCAAAACCGTGCTTGACAGTATGGACGGCttttt < 1:2834130/68‑1 (MQ=255) gTGATGGGCCGCGAAGTGGTGGAAAAATTAACCGGCAAAACCGTGCTTGACAGTATGGACGGCttttt < 1:281987/68‑1 (MQ=255) gTGATGGGCCGCGAAGTGGTGGAAAAATTAACCGGCAAAACCGTGCTTGACAGTATGGACGGCttttt < 1:2763205/68‑1 (MQ=255) gTGATGGGCCGCGAAGTGGTGGAAAAATTAACCGGCAAAACCGTGCTTGACAGTATGGACGGCttttt < 1:12740/68‑1 (MQ=255) gTGATGGGCCGCGAAGTGGTGGAAAAATTAACCGGCAAAACCGTGCTTGACAGTATGGACGGCttttt > 1:2592435/1‑68 (MQ=255) gTGATGGGCCGCGAAGTGGTGGAAAAATTAACCGGCAAAACCGTGCTTGACAGTATGGACGGCttttt > 1:1440000/1‑68 (MQ=255) gTGATGGGCCGCGAAGTGGTGGAAAAATTAACCGGCAAAACCGTGCTTGACAGTATGGACGGCttttt < 1:1922304/68‑1 (MQ=255) gTGATGGGCCGCGAAGTGGTGGAAAAATTAACCGGCAAAACCGTGCTTGACAGTATGGACGGCttttt < 1:1938157/68‑1 (MQ=255) gTGATGGGCCGCGAAGTGGTGGAAAAATTAACCGGCAAAACCGTGCTTGACAGTATGGACGGCttttt < 1:2181323/68‑1 (MQ=255) gTGATAGGCCGCGAAGTGGTGGAAAAATTAACCGGCAAAACCGTGCTTGACAGTATGGACGGCttttt < 1:2479553/68‑1 (MQ=255) gCCGCGAAGTGGTGGAAAAATTAACCGGCAAAACCGTGCTTGACAGTATGGACGGCTTTTTCACTCGCt > 1:1293461/1‑69 (MQ=255) gCCGCGAAGTGGTGGAAAAATTAACCGGCAAAACCGTGCTTGACAGTATGGACGGCTTTTTCACTCGCt > 1:3279035/1‑69 (MQ=255) gcgAAGTGGTGGAAAAATTAACCGGCAAAACCGTGCTTGACAGTATGGACGGCTTTTTCACTCGCTACg < 1:2592392/69‑1 (MQ=255) gtggAAAAATTAACCGGCAAAACCGTGCTTGACAGTATGGACGGCTTTTTCACTCGCTACGGCAAacac > 1:1362576/1‑69 (MQ=255) gtggAAAAATTAACCGGCAAAACCGTACTTGACAGTATGGACGGCTTTTTCACTCGTTACGGCAAacac > 1:429153/1‑69 (MQ=255) gtggAAAAATTAACCGGCAAAACCGTACTTGACAGTATGGACGGCTTTTTCACTCGTTACGGCAAacac > 1:1331707/1‑69 (MQ=255) gtggAAAAATTAACCGGCAAAACCGTACTTGACAGTATGGACGGCTTTTTCACTCGTTACGGCAAacac > 1:830142/1‑69 (MQ=255) aaaaCCGTGCTTGACAGTATGGACGGCTTTTTCACTCGCTACGGCAAACACAcc > 1:2314380/1‑54 (MQ=255) aaaaCCGTGCTTGACAGTATGGACGGCTTTTTCACTCGCTACGGCAAACACACCATTCTGGTCTGTCg > 1:2299559/1‑68 (MQ=255) aaaaCCGTGCTTGACAGTATGGACGGCTTTTTCACTCGCTACGACAAACACACCATTCTGGTCTGTCg > 1:2741583/1‑68 (MQ=255) aaaCCGTGCTTGACAGTATGGACGGCTTTTTCACTCGCTACGGCAAACACACCATTCTGGTCTGTCGGt < 1:1849478/69‑1 (MQ=255) aaaCCGTGCTTGACAGTATGGACGGCTTTTTCACTCGCTACGGCAAACACACCATTCTGGTCTGTCGGt < 1:1328898/69‑1 (MQ=255) aaCCGTGCTTGACAGTATGGACGGCTTTTTCACTCGCTACGGCAAACACACCATTCTGGTCTGTCGGt < 1:2364919/68‑1 (MQ=255) | CTTTTTTATCGCCAGAGTGATGGGCCGCGAAGTGGTGGAAAAATTAACCGGCAAAACCGTGCTTGACAGTATGGACGGCTTTTTCACTCGCTACGGCAAACACACCATTCTGGTCTGTCGGT > minE/1162837‑1162958 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |