| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 96,629 | C→T | 100% | P237P (CCG→CCA) | yacF ← | conserved hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 96,629 | 0 | C | T | 82.4% | 67.4 / 6.4 | 45 | P237P (CCG→CCA) | yacF | conserved hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base C (7/1); new base T (37/0); total (44/1) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.78e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
ATTCACCGTAATAGTTTCTGACATTTTTACTCCTTAGCAACAGGCCAGTTCGAAATCCAGACGTTCCGGTACCTGTCCGTTTTCAGTGTCCAGCGGCATAAAACGAATGGCAAAA > minE/96563‑96677 | aTTCACCGTAATAGTTTCTGACATTTTTACTCCTTAGCAACAGGCCAGTTCGAAATCCAGACGTTCTgg > 1:2383596/1‑69 (MQ=255)aTTCACCGTAATAGTTTCTGACATTTTTACTCCTTAGCAACAGGCCAGTTCGAAATCCAGACGTTCTgg > 1:96343/1‑69 (MQ=255)aTTCACCGTAATAGTTTCTGACATTTTTACTCCTTAGCAACAGGCCAGTTCGAAATCCAGACGTTCTgg > 1:3050772/1‑69 (MQ=255)aTTCACCGTAATAGTTTCTGACATTTTTACTCCTTAGCAACAGGCCAGTTCGAAATCCAGACGTTCTgg > 1:2709396/1‑69 (MQ=255)aTTCACCGTAATAGTTTCTGACATTTTTACTCCTTAGCAACAGGCCAGTTCGAAATCCAGACGTTCTgg > 1:2556140/1‑69 (MQ=255)aTTCACCGTAATAGTTTCTGACATTTTTACTCCTTAGCAACAGGCCAGTTCGAAATCCAGACGTTCTgg > 1:1990024/1‑69 (MQ=255)aTTCACCGTAATAGTTTCTGACATTTTTACTCCTTAGCAACAGGCCAGTTCGAAATCCAGACGTTCTgg > 1:1834211/1‑69 (MQ=255)aTTCACCGTAATAGTTTCTGACATTTTTACTCCTTAGCAACAGGCCAGTTCGAAATCCAGACGTTCTgg > 1:1197928/1‑69 (MQ=255) cACCGTAATAGTTTCTGACATTTTTACTCCTTAGCAACAGGCCAGTTCGAAATCCAGACGTTCCGGTAc > 1:661023/1‑69 (MQ=255) cACCGTAATAGTTTCTGACATTTTTACTCCTTAGCAACAGGCCAGTTCGAAATCCAGACGTTCCGGTAc < 1:2135804/69‑1 (MQ=255) tCTGACATTTTTACTCCTTAGCAACAGGCCAGTTCGAAATCCAGACGTTCCGGTACCTGTCCGTTTTc > 1:1761715/1‑68 (MQ=255) tCTGACATTTTTACTCCTTAGCAACAGGCCAGTTCGAAATCCAGACGTTCCGGTACCTGTCCGTTTTCa > 1:70551/1‑69 (MQ=255) tCTGACATTTTTACTCCTTAGCAACAGGCCAGTTCGAAATCCAGACGTTCCGGTACCTGTCCGTTTTCa > 1:1823630/1‑69 (MQ=255) tCTGACATTTTTACTCCTTAGCAACAGGCCAGTTCGAAATCCAGACGTTCCGGTACCTGTCCGTTTTCa > 1:595234/1‑69 (MQ=255) tCTGACATTTTTACTCCTTAGCAACAGGCCAGTTCGAAATCCAGACGTTCCGGTACCTGTCCGTTTTCa > 1:1180688/1‑69 (MQ=255) tCTGACATTTTTACTCCTTAGCAACAGGCCAGTTCGAAATCCAGACGTTCCGGTACCTGTCCGTTTTCa > 1:1063366/1‑69 (MQ=255) cAGTTCGAAATCCAGACGTTCTGGTACCTGTCCGTTTTCACTGTCCAGCGGCATAAAACGAATGGGaaa > 1:593225/1‑69 (MQ=255) cAGTTCGAAATCCAGACGTTCTGGTACCTGTCCGTTTTCACTGTCCAGCGGCATAAAACGAATGGCaaa > 1:409522/1‑69 (MQ=255) cAGTTCGAAATCCAGACGTTCTGGTACCTGTCCGTTTTCACTGTCCAGCGGCATAAAACGAATGGCaaa > 1:1495104/1‑69 (MQ=255) cAGTTCGAAATCCAGACGTTCTGGTACCTGTCCGTTTTCACTGTCCAGCGGCATAAAACGAATGGCaaa > 1:2811762/1‑69 (MQ=255) cAGTTCGAAATCCAGACGTTCTGGTACCTGTCCGTTTTCACTGTCCAGCGGCATAAAACGAATGGCaaa > 1:3017058/1‑69 (MQ=255) cAGTTCGAAATCCAGACGTTCTGGTACCTGTCCGTTTTCACTGTCCAGCGGCATAAAACGAATGGCaaa > 1:3352220/1‑69 (MQ=255) cAGTTCGAAATCCAGACGTTCTGGTACCTGTCCGTTTTCACTGTCCAGCGGCATAAAACGAATGGCaaa > 1:2566303/1‑69 (MQ=255) cAGTTCGAAATCCAGACGTTCTGGTACCTGTCCGTTTTCACTGTCCAGCGGCATAAAACGAATGGCaaa > 1:1060800/1‑69 (MQ=255) cAGTTCGAAATCCAGACGTTCTGGTACCTGTCCGTTTTCACTGTCCAGCGGCATAAAACGAATGGCaaa > 1:2477692/1‑69 (MQ=255) cAGTTCGAAATCCAGACGTTCTGGTACCTGTCCGTTTTCACTGTCCAGCGGCATAAAACGAATGGCaaa > 1:1575641/1‑69 (MQ=255) cAGTTCGAAATCCAGACGTTCTGGTACCTGTCCGTTTTCACTGTCCAGCGGCATAAAACGAATGGCaaa > 1:1627587/1‑69 (MQ=255) cAGTTCGAAATCCAGACGTTCTGGTACCTGTCCGTTTTCACTGTCCAGCGGCATAAAACGAATGGCaaa > 1:1290548/1‑69 (MQ=255) cAGTTCGAAATCCAGACGTTCTGGTACCTGTCCGTTTTCACTGTCCAGCGGCATAAAACGAATGGCaaa > 1:1278684/1‑69 (MQ=255) cAGTTCGAAATCCAGACGTTCTGGTACCTGTCCGTTTTCACTGTCCAGCGGCATAAAACGAATGGCaaa > 1:2114372/1‑69 (MQ=255) cAGTTCGAAATCCAGACGTTCTGGTACCTGTCCGTTTTCACTGTCCAGCGGCATAAAACGAATGGCaaa > 1:1994847/1‑69 (MQ=255) cAGTTCGAAATCCAGACGTTCTGGTACCTGTCCGTTTTCACTGTCCAGCGGCATAAAACGAATGGCaa > 1:1001274/1‑68 (MQ=255) cAGTTCGAAATCCAGACGTTCTGGTACCTGTCCGTTTTCACTGTCCAGCGGCATAAAACGAATGGCaa > 1:405150/1‑68 (MQ=255) aGTTCGAAATCCAGACGTTCTTGTACCTGTCCGTTTTCACTGTCCAGCGGCATAAAACGAATGGCaaaa > 1:2445112/1‑69 (MQ=255) aGTTCGAAATCCAGACGTTCTGGTACCTGTCCGTTTTCACTGTCCAGCGGCATAAAACGAATGGCaaaa > 1:3321179/1‑69 (MQ=255) aGTTCGAAATCCAGACGTTCTGGTACCTGTCCGTTTTCACTGTCCAGCGGCATAAAACGAATGGCaaaa > 1:3061779/1‑69 (MQ=255) aGTTCGAAATCCAGACGTTCTGGTACCTGTCCGTTTTCACTGTCCAGCGGCATAAAACGAATGGCaaaa > 1:3422043/1‑69 (MQ=255) aGTTCGAAATCCAGACGTTCTGGTACCTGTCCGTTTTCACTGTCCAGCGGCATAAAACGAATGGCaaaa > 1:2064336/1‑69 (MQ=255) aGTTCGAAATCCAGACGTTCTGGTACCTGTCCGTTTTCACTGTCCAGCGGCATAAAACGAATGGCaaaa > 1:2214083/1‑69 (MQ=255) aGTTCGAAATCCAGACGTTCTGGTACCTGTCCGTTTTCACTGTCCAGCGGCATAAAACGAATGGCaaaa > 1:1890583/1‑69 (MQ=255) aGTTCGAAATCCAGACGTTCTGGTACCTGTCCGTTTTCACTGTCCAGCGGCATAAAACGAATGGCaaaa > 1:2717120/1‑69 (MQ=255) aGTTCGAAATCCAGACGTTCTGGTACCTGTCCGTTTTCACTGTCCAGCGGCATAAAACGAATGGCaaaa > 1:1692995/1‑69 (MQ=255) aGTTCGAAATCCAGACGTTCTGGTACCTGTCCGTTTTCACTGTCCAGCGGCATAAAACGAATGGCaaaa > 1:2230702/1‑69 (MQ=255) aGTTCGAAATCCAGACGTTCTGGTACCTGTCCGTTTTCACTGTCCAGCGGCATAAAACGAATGGCaaaa > 1:25706/1‑69 (MQ=255) aGTTCGAAATCCAGACGTTCTGGTACCTGTCCGTTTTCACTGTCCAGAGGCATAAAACGAATGGCaaaa > 1:613682/1‑69 (MQ=255) | ATTCACCGTAATAGTTTCTGACATTTTTACTCCTTAGCAACAGGCCAGTTCGAAATCCAGACGTTCCGGTACCTGTCCGTTTTCAGTGTCCAGCGGCATAAAACGAATGGCAAAA > minE/96563‑96677 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |