| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,170,190 | A→C | 30.4% | P43P (CCA→CCC) | gdhA → | glutamate dehydrogenase, NADP‑specific |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,170,190 | 0 | A | C | 30.4% | 46.0 / 27.2 | 46 | P43P (CCA→CCC) | gdhA | glutamate dehydrogenase, NADP‑specific |
| Reads supporting (aligned to +/- strand): ref base A (9/23); new base C (7/7); total (16/30) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.89e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.97e-01 | |||||||||||
AAACCGAGTTCGCGCAAGCCGTTCGTGAAGTAATGACCACACTCTGGCCTTTTCTTGAACAAAATCCAAAATATCGCCAGATGTCATTACTGGAGCGTCTGGTTGAACCGGAGCGCGTGATCCAGTTTCGCGTGG > minE/1170123‑1170257 | aaaCCGAGTTCGCGCAAGCCGTTCGTGAAGTAATGACCACACTCTGGCCTTTTCTTGAACAAAATCCaa < 1:2980359/69‑1 (MQ=255)aaaCCGAGTTCGCGCAAGCCGTTCGTGAAGTAATGACCACACTCTGGCCTTTTCTTGAACAAAATCCaa < 1:2068559/69‑1 (MQ=255)aaaCCGAGTTCGCGCAAGCCGTTCGTGAAGTAATGACCACACTCTGGCCTTTTCTTGAACAAAATCCaa < 1:692587/69‑1 (MQ=255)aaaCCGAGTTCGCGCAAGCCGTTCGTGAAGTAATGACCACACTCTGGCCTTTTCTTGAACAAAATCCaa < 1:225572/69‑1 (MQ=255)aaaCCGAGTTCGCGCAAGCCGTTCGTGAAGTAATGACCACACTCTGGCCTTTTCTTGAACAAAATCCaa < 1:1670588/69‑1 (MQ=255)aaaCCGAGTTCGCGCAAGCCGTTCGTGAAGTAATGACCACACTCTGGCCTTTTCTTGAACAAAATCCaa < 1:2361845/69‑1 (MQ=255)aaaCCGAGTTCGCGCAAGCCGTTCGTGAAGTAATGACCACACTCTGGCCTTTTCTTGAACAAAATCCaa < 1:3336105/69‑1 (MQ=255)aaaCCGAGTTCGCGCAAGCCGTTCGTGAAGTAATGACCACACTCTGGCCTTTTCTTGAACAAAATCCaa < 1:1320768/69‑1 (MQ=255) aCCGAGTTCGCGCAAGCCGTTCGTGAAGTAATGACCACACTCTGGCCTTTTCTTGAACAAAATCCaaaa < 1:1482901/69‑1 (MQ=255) aCCGAGTTCGCGCAAGCCGTTCGTGAAGTAATGACCACACTCTGGCCTTTTCTTGAACAAAATCCaaaa < 1:978667/69‑1 (MQ=255) aCCGAGTTCGCGCAAGCCGTTCGTGAAGTAATGACCACACTCTGGCCTTTTCTTGAACAAAATCCaaaa < 1:2733917/69‑1 (MQ=255) cgcgCAAGCCGTTCGTGAAGTAATGACCACACTCTGGCCTTTTCTTGAACAAAATCCAAAatat > 1:810968/1‑64 (MQ=255) cgcgCAAGCCGTTCGTGAAGTAATGACCACACTCTGGCCTTTTCTTGAACAAAATCCAAAATATc > 1:348639/1‑65 (MQ=255) cgcgCAAGCCGTTCGTGAAGTAATGACCACACTCTGGCCTTTTCTTGAACAAAATCCAAAATATc > 1:1446009/1‑65 (MQ=255) cgcgCAAGCCGTTCGTGAAGTAATGACCACACTCTGGCCTTTTCTTGAACAAAATCCAAAATATc > 1:1484572/1‑65 (MQ=255) cgcgCAAGCCGTTCGTGAAGTAATGACCACACTCTGGCCTTTTCTTGAACAAAATCCAAAATATc > 1:2897282/1‑65 (MQ=255) gcAAGCCGTTCGTGAAGTAATGACCACACTCTGGCCTTTTCTTGAACAAAATCCAAAATATCGCCAGAt < 1:2189208/69‑1 (MQ=255) gcAAGCCGTTCGTGAAGTAATGACCACACTCTGGCCTTTTCTTGAACAAAATCCAAAATATCGCCAGAt < 1:1054553/69‑1 (MQ=255) gcAAGCCGTTCGTGAAGTAATGACCACACTCTGGCCTTTTCTTGAACAAAATCCAAAATATCGCCAGAt < 1:1225099/69‑1 (MQ=255) gcAAGCCGTTCGTGAAGTAATGACCACACTCTGGCCTTTTCTTGAACAAAATCCAAAATATCGCCAGAt < 1:932175/69‑1 (MQ=255) gcAAGCCGTTCGTGAAGT‑ATGACCACACTCTGGCCTTTTCTTGAACAAAATCCAAAATATCGCCAGAt < 1:3016157/68‑1 (MQ=255) aaGCCGTTCGTGAAGTAATGACCACACTCTGGCCTTTTCTTGAACAAAATCCaaaa < 1:2734049/56‑1 (MQ=255) aaGCCGTTCGTGAAGTAATGACCACACTCTGGCCTTTTCTTGAACAAAATCCAAAATATCGCCAGATGt < 1:2046233/69‑1 (MQ=255) cGTGAAGTAATGACCACACTCTGGCCTTTTCTTGAACAAAATCCCAAATATCGTCAGATGTCATTAc > 1:1994602/1‑67 (MQ=255) cGTGAAGTAATGACCACACTCTGGCCTTTTCTTGAACAAAATCCCAAATATCGTCAGATGTCATTAc > 1:2648505/1‑67 (MQ=255) cGTGAAGTAATGACCACACTCTGGCCTTTTCTTGAACAAAATCCCAAATATCGTCAGATGTCATTAc > 1:141625/1‑67 (MQ=255) cGTGAAGTAATGACCACACTCTGGCCTTTTCTTGAACAAAATCCCAAATATCGTCAGATGTCATTAc > 1:2391728/1‑67 (MQ=255) cGTGAAGTAATGACCACACTCTGGCCTTTTCTTGAACAAAATCCCAAATATCGTCAGATGTCATTAc > 1:1788722/1‑67 (MQ=255) cGTGAAGTAATGACCACACTCTGGCCTTTTCTTGAACAAAATCCCAAATATCGTCAGATGTCATTAc > 1:525626/1‑67 (MQ=255) cGTGAAGTAATGACCACACTCTGGCCTTTTCTCGAACAAAATCCCAAATATCGTCAGATGTCATTAc > 1:602639/1‑67 (MQ=255) gTAATGACCACACTCTGGCCTTTTCTTGAACAAAATCCCAAATATCGTCAGATGTCATTACTGGAGCg < 1:1741466/68‑1 (MQ=255) gTAATGACCACACTCTGGCCTTTTCTTGAACAAAATCCCAAATATCGTCAGATGTCATTACTGGAGCg < 1:2614394/68‑1 (MQ=255) gTAATGACCACACTCTGGCCTTTTCTTGAACAAAATCCCAAATATCGTCAGATGTCATTACTGGAGCg < 1:2828766/68‑1 (MQ=255) gTAATGACCACACTCTGGCCTTTTCTTGAACAAAATCCCAAATATCGTCAGATGTCATTACTGGAGCg < 1:2830381/68‑1 (MQ=255) gTAATGACCACACTCTGGCCTTTTCTTGAACAAAATCCCAAATATCGTCAGATGTCATTACTGGAGCg < 1:1202320/68‑1 (MQ=255) tAATGACCACACTCTGGCCTTTTCTTGAACAAAATCCCAAATATCGTCAGATGTCATTACTGGAGCg < 1:709445/67‑1 (MQ=255) aTGACCACACTCTGGCCTTTTCTTGAACAAAATCCAAAATATCGCCAGATGTCATTACTGGAGCGTCTg > 1:484075/1‑69 (MQ=255) aTGACCACACTCTGGCCTTTTCTTGAACAAAATCCAAAATATCGCCAGATGTCATTACTGGAGCGTCTg > 1:2243489/1‑69 (MQ=255) cacaCTCTGGCCTTTTCTTGAACAAAATCCAAAATATCGCCAGATGTCATTACTGGAGCGTCTGGTTGa < 1:3337504/69‑1 (MQ=255) cacaCTCTGGCCTTTTCTTGAACAAAATCCAAAATATCGCCAGATGTCATTACTGGAGCGTCTGGTTGa < 1:3127649/69‑1 (MQ=255) cacaCTCTGGCCTTTTCTTGAACAAAATCCAAAATATCGCCAGATGTCATTACTGGAGCGTCTGGTTGa < 1:298435/69‑1 (MQ=255) cacaCTCTGGCCTTTTCTTGAACAAAATCCAAAATATCGCCAGATGTCATTACTGGAGCGTCTGGTTGa < 1:110627/69‑1 (MQ=255) cacaCTCTGGCCTTTTCTTGAACAAAATCCAAAATATCGCCAGATGTCATTACTGGAGCGTCTGGTTGa < 1:276975/69‑1 (MQ=255) cacaCTCTGGCCTTTTCTTGAACAAAATCCAAAATATCGCCAGATGTCATTACTGGAGCGTCTGGTTGa < 1:1335055/69‑1 (MQ=255) cacaCTCTGGCCTTTTCTTGAACAAAATCCAAAATATCGCCAGATGTCATTACTGGAGCGTCTGGTTGa < 1:1546615/69‑1 (MQ=255) cacTCTGGCCTTTTCTTGAACAAAATCCAAAATATCGCCAGATGTCATTACTGGAGCGTCTGGTTGAAc < 1:3249411/69‑1 (MQ=255) cacTCTGGCCTTTTCTTGAACAAAATCCAAAATATCGCCAGATGTCATTACTGGAGCGTCTGGTTGAAc < 1:2438054/69‑1 (MQ=255) cacTCTGGCCTTTTCTTGAACAAAATCCAAAATATCGCCAGATGTCATTACTGGAGCGTCTGGTTGAAc < 1:1619628/69‑1 (MQ=255) cacTCTGGCCTTTTCTTGAACAAAATCCAAAATATCGCCAGATGTCATTACTGGAGCGTCTGGTTGAAc < 1:2129622/69‑1 (MQ=255) acTCTGGCCTTTTCTTGAACAAAATCCAAAATATCGCCAGATGTCATTACTGGAGCGTCTGGTTGAAc < 1:2271957/68‑1 (MQ=255) tctGGCCTTTTCTTTAACAAAATCCCAAATATCGTCAGATGTCATTACTGGAGCg < 1:1243296/55‑1 (MQ=255) cTTTTCTTGAACAAAATCCAAAATATCGCCAGATGTCATTACTGGAGCGTCTGGTTGAACCGGAgcgc > 1:2422019/1‑68 (MQ=255) cTTTTCTTGAACAAAATCCAAAATATCGCCAGATGTCATTACTGGAGCGTCTGGTTGAACCGGAgcgc > 1:1862881/1‑68 (MQ=255) cAAAATATCGCCAGATGTCATTACTGGAGCGTCTGGTTGAACCGGAGCGCGTGATCCAGTTTCGCGTgg < 1:2076667/69‑1 (MQ=255) cAAAATATCGCCAGATGTCATTACTGGAGCGTCTGGTTGAACCGGAGCGCGTGATCCAGTTTCGCGTgg < 1:3386739/69‑1 (MQ=255) cAAAATATCGCCAGATGTCATTACTGGAGCGTCTGGTTGAACCGGAGCGCGTGATCCAGTTTCGCGTgg < 1:2509516/69‑1 (MQ=255) cAAAATATCGCCAGATGTCATTACTGGAGCGTCTGGTTGAACCGGAGCGCGTGATCCAGTTTCGCGTgg < 1:2730289/69‑1 (MQ=255) cAAAATATCGCCAGATGTCATTACTGGAGCGTCTGGTTGAACCGGAGCGCGTGATCCAGTTTCGCGTgg < 1:2776312/69‑1 (MQ=255) aaaaTATCGCCAGATGTCATTACTGGAGCGTCTGGTTGAACCGGAGCGCGTGATCCAGTTTCGCGTgg < 1:468876/68‑1 (MQ=255) | AAACCGAGTTCGCGCAAGCCGTTCGTGAAGTAATGACCACACTCTGGCCTTTTCTTGAACAAAATCCAAAATATCGCCAGATGTCATTACTGGAGCGTCTGGTTGAACCGGAGCGCGTGATCCAGTTTCGCGTGG > minE/1170123‑1170257 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |