Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	minE	1,174,406	G→A	63.3%	T82T (ACC→ACT)	topB ←	DNA topoisomerase III

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	1,174,406	0	G	A	63.3%	37.6 / 40.9	68	T82T (ACC→ACT)	topB	DNA topoisomerase III
Reads supporting (aligned to +/- strand):  ref base G (7/18);  new base A (15/28);  total (22/46)
Fisher's exact test for biased strand distribution p-value = 6.02e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TCCCCGGCGTGAACGATTTCGCTGGCTTCATGCAGGAACCGTTTGATGACGTTAAGTTGTTTGGTCACGGAGGGTCGGGGCTGTAATTGCCACTTTTCCGGGACAATCGGCAAATCCGCAAGATTCC  >  minE/1174344‑1174470                                                               |                                                                 tCCCCGGCGTGAACGATTTCGCTGGCTTCATGCAGGAACCGTTTGATGACGTTAAGTTGTTTGGTCACg                                                            <  1:1862382/69‑1 (MQ=255)  ccccGGCGTGAACGATTTCGCTGGCTTCATGCAGGAACCGTTTGATGACGTTAAGTTGTTTGGTCACg                                                            <  1:2897281/68‑1 (MQ=255)              cGATTTCGCTGGCTTCATGCAGGAACCGTTTGATGACGTTAAGTTGTTTGGTCACGGAGGGTCGGGGc                                                <  1:3383751/68‑1 (MQ=255)                aTTTCGCTGGCTTCATGCAGGAACCGTTTGATGACGTTAAGTTGTTTGGTCACGGAGGGTCGGGGCTGt                                             <  1:1271142/69‑1 (MQ=255)                aTTTCGCTGGCTTCATGCAGGAACCGTTTGATGACGTTAAGTTGTTTGGTCACGGAGGGTCGGGGCTGt                                             <  1:1315614/69‑1 (MQ=255)                aTTTCGCTGGCTTCATGCAGGAACCGTTTGATGACGTTAAGTTGTTTGGTCACGGAGGGTCGGGGCTGt                                             <  1:965513/69‑1 (MQ=255)                aTTTCGCTGGCTTCATGCAGGAACCGTTTGATGACGTTAAGTTGTTTGGTCACGGAGGGTCGGGGCTGt                                             <  1:899957/69‑1 (MQ=255)                aTTTCGCTGGCTTCATGCAGGAACCGTTTGATGACGTTAAGTTGTTTGGTCACGGAGGGTCGGGGCTGt                                             <  1:2508816/69‑1 (MQ=255)                aTTTCGCTGGCTTCATGCAGGAACCGTTTGATGACGTTAAGTTGTTTGGTCACGGAGGGTCGGGGCTGt                                             <  1:2716038/69‑1 (MQ=255)                aTTTCGCTGGCTTCATGCAGGAACCGTTTGATGACGTTAAGTTGTTTGGTCACGGAGGGTCGGGGCTGt                                             <  1:1505487/69‑1 (MQ=255)                aTTTCGCTGGCTTCATGCAGGAACCGTTTGATGACGTTAAGTTGTTTGGTCACGGAGGGTCGGGGCTGt                                             <  1:2784445/69‑1 (MQ=255)                aTTTCGCTGGCTTCATGCAGGAACCGTTTGATGACGTTAAGTTGTTTGGTCACGGAGGGTCGGGGCTGt                                             <  1:2104709/69‑1 (MQ=255)                aTTTCGCTGGCTTCATGCAGGAACCGTTTGATGACGTTAAGTTGTTTGGTCACGGAGGGTCGGGGCTGt                                             <  1:621630/69‑1 (MQ=255)                aTTTCGCTGGCTTCATGCAGGAACCGTTTGATGACGTTAAGTTGTTTGGTCACGGAGGGTCGGGGCTGt                                             <  1:568514/69‑1 (MQ=255)                aTTTCGCTGGCTTCATGCAGGAACCGTTTGATGACGTTAAGTTGTTTGGTCACGGAGGGTCGGGGCTGt                                             <  1:2849033/69‑1 (MQ=255)                aTTTCGCTGGCTTCATGCAGGAACCGTTTGATGACGTTAAGTTGTTTGGTCACGGAGGGTCGGGGCTGt                                             <  1:1710110/69‑1 (MQ=255)                aTTTCGCTGGCTTCATGCAGGAACCGTTTGATGACGTTAAGTTGTTTGGTCACGGAGGGTCGGGGCTGt                                             <  1:2903117/69‑1 (MQ=255)                aTTTCGCTGGCTTCATGCAGGAACCGTTTGATGACGTTAAGTTGTTTGGTCACGGAGGGTCGGGGCTGt                                             <  1:1793387/69‑1 (MQ=255)                       tGGCTTCATGCAGGAAGTGTTTGATGACATTAAGTTGTTTAGTCACTGAGGGTCGGGGCTGTAATTGc                                       <  1:1936823/68‑1 (MQ=255)                       tGGCTTCATGCAGGAAGTGTTTGATGACATTAAGTTGTTTAGTCACGGAGGGt                                                      >  1:1979925/1‑53 (MQ=255)                       tGGCTTCATGCAGGAAGTGTTTGATGACATTAAGTTGTTTAGTCACGGAGGGTCGGGGc                                                >  1:1259539/1‑59 (MQ=255)                       tGGCTTCATGCAGGAAGTGTTTGATGACATTAAGTTGTTTAGTCACGGAGGGTCGGGGCTGt                                             >  1:2852130/1‑62 (MQ=255)                       tGGCTTCATGCAGGAAGTGTTTGATGACATTAAGTTGTTTAGTCACGGAGGGTCGGGGCTGTAATTGcc                                      >  1:1995488/1‑69 (MQ=255)                       tGGCTTCATGCAGGAAGTGTTTGATGACATTAAGTTGTTTAGTCACGGAGGGTCGGGGCTGTAATTGcc                                      >  1:202839/1‑69 (MQ=255)                       tGGCTTCATGCAGGAAGTGTTTGATGACATTAAGTTGTTTAGTCACGGAGGGTCGGGGCTGTAATTGcc                                      >  1:2444432/1‑69 (MQ=255)                       tGGCTTCATGCAGGAAGTGTTTGATGACATTAAGTTGTTTAGTCACGGAGGGTCGGGGCTGTAATTGcc                                      >  1:992034/1‑69 (MQ=255)                       tGGCTTCATGCAGGAAGTGTTTGATGACATTAAGTTGTTTAGTCACGGAGGGTCGGGGCTGTAATTGcc                                      >  1:3354865/1‑69 (MQ=255)                       tGGCTTCATGCAGGAAGTGTTTGATGACATTAAGTTGTTTAGTCACGGAGGGTCGGGGCTGTAATTGcc                                      >  1:340792/1‑69 (MQ=255)                       tGGCTTCATGCAGGAAGTGTTTGATGACATTAAGTTGTTTAGTCACGGAGGGTCGGGGCTGTAATTGcc                                      >  1:3428164/1‑69 (MQ=255)                       tGGCTTCATGCAGGAAGTGTTTGATGACATTAAGTTGTTTAGTCACGGAGGGTCGGGGCTGTAATTGcc                                      >  1:635014/1‑69 (MQ=255)                       tGGCTTCATGCAGGAAGTGTTTGATGACATTAAGTTGTTTAGTCACGGAGGGTCGGGGCTGTAATTGcc                                      >  1:1526376/1‑69 (MQ=255)                       tGGCTTCATGCAGGAAGTGTTTGATGACATTAAGTTGTTTAGTCACGGAGGGTCGGGGCTGTAATTGcc                                      >  1:670595/1‑69 (MQ=255)                       tGGCTTCATGCAGGAAGTGTTTGATGACATTAAGTTGTTTAGTCACGGAGGGTCGGGGCTGTAATTGcc                                      >  1:869289/1‑69 (MQ=255)                       tGGCTTCATGCAGGAAGTGTTTGATGACATTAAGTTGTTTAGTCACGGAGGGTCGGGGCTGTAATTGc                                       <  1:356672/68‑1 (MQ=255)                       tGGCTTCATGCAGGAAGTGTTTGATGACATTAAGTTGTTTAGTCACGGAGGGTCGGGGCTGTAATTGc                                       <  1:1147419/68‑1 (MQ=255)                       tGGCTTCATGCAGGAAGTGTTTGATGACATTAAGTTGTTTAGTCACGGAGGGTCGGGGCTGTAATTGc                                       <  1:24348/68‑1 (MQ=255)                       tGGCTTCATGCAGGAAGTGTTTGATGACATTAAGTTGTTTAGTCACGGAGGGTCGGGGCTGTAATTGc                                       <  1:1326948/68‑1 (MQ=255)                       tGGCTTCATGCAGGAAGTGTTTGATGACATTAAGTTGTTTAGTCACGGAGGGTCGGGGCTGTAATTGc                                       <  1:2163759/68‑1 (MQ=255)                       tGGCTTCATGCAGGAAGTGTTTGATGACATTAAGTTGTTTAGTCACGGAGGGTCGGGGCTGTAATTGc                                       <  1:2117965/68‑1 (MQ=255)                       tGGCTTCATGCAGGAAGTGTTTGATGACATTAAGTTGTTTAGTCACGGAGGGTCGGGGCTGTAATTGc                                       <  1:2105569/68‑1 (MQ=255)                       tGGCTTCATGCAGGAAGTGTTTGATGACATTAAGTTGTTTAGTCACGGAGGGTCGGGGCTGTAATTGc                                       <  1:1890903/68‑1 (MQ=255)                       tGGCTTCATGCAGGAAGTGTTTGATGACATTAAGTTGTTTAGTCACGGAGGGTCGGGGCTGTAATTGc                                       <  1:1889519/68‑1 (MQ=255)                       tGGCTTCATGCAGGAAGTGTTTGATGACATTAAGTTGTTTAGTCACGGAGGGTCGGCGCTGTAATTGc                                       >  1:2945658/1‑68 (MQ=255)                               tGCAGGAAGTGTTTGATGACATTAAGTTGTTTAGTCACGGAGGGTCGGGGCTGTAATTGCCACTTTTc                               <  1:632908/68‑1 (MQ=255)                               tGCAGGAAGTGTTTGATGACATTAAGTTGTTTAGTCACGGAGGGTCGGGGCTGTAATTGCCACTTTTc                               <  1:1771559/68‑1 (MQ=255)                               tGCAGGAAGTGTTTGATGACATTAAGTTGTTTAGTCACGGAGGGTCGGGGCTGTAATTGCCACTTTTc                               <  1:1605011/68‑1 (MQ=255)                               tGCAGGAAGTGTTTGATGACATTAAGTTGTTTAGTCACGGAGGGTCGGGGCTGTAATTGCCACTTTTc                               <  1:55894/68‑1 (MQ=255)                               tGCAGGAAGTGTTTGATGACATTAAGTTGTTTAGTCACGGAGGGTCGGGGCTGTAATTGCCACTTTTc                               <  1:1599590/68‑1 (MQ=255)                               tGCAGGAAGTGTTTGATGACATTAAGTTGTTTAGTCACGGAGGGTCGGGGCTGTAATTGCCACTTTTc                               <  1:1585634/68‑1 (MQ=255)                               tGCAGGAAGTGTTTGATGACATTAAGTTGTTTAGTCACGGAGGGTCGGGGCTGTAATTGCCACTTTTc                               <  1:235572/68‑1 (MQ=255)                               tGCAGGAAGTGTTTGATGACATTAAGTTGTTTAGTCACGGAGGGTCGGGGCTGTAATTGCCACTTTTc                               <  1:1560293/68‑1 (MQ=255)                               tGCAGGAAGTGTTTGATGACATTAAGTTGTTTAGTCACGGAGGGTCGGGGCTGTAATTGCCACTTTTc                               <  1:1417048/68‑1 (MQ=255)                               tGCAGGAAGTGTTTGATGACATTAAGTTGTTTAGTCACGGAGGGTCGGGGCTGTAATTGCCACTTTTc                               <  1:1008325/68‑1 (MQ=255)                               tGCAGGAAGTGTTTGATGACATTAAGTTGTTTAGTCACGGAGGGTCGGGGCTGTAATTGCCACTTTTc                               <  1:1833679/68‑1 (MQ=255)                               tGCAGGAAGTGTTTGATGACATTAAGTTGTTTAGTCACGGAGGGTCGGGGCTGTAATTGCCACTTTTc                               <  1:1852160/68‑1 (MQ=255)                               tGCAGGAAGTGTTTGATGACATTAAGTTGTTTAGTCACGGAGGGTCGGGGCTGTAATTGCCACTTTTc                               <  1:3203168/68‑1 (MQ=255)                               tGCAGGAAGTGTTTGATGACATTAAGTTGTTTAGTCACGGAGGGTCGGGGCTGTAATTGCCACTTTTc                               <  1:2990708/68‑1 (MQ=255)                               tGCAGGAAGTGTTTGATGACATTAAGTTGTTTAGTCACGGAGGGTCGGGGCTGTAATTGCCACTTTTc                               <  1:1913468/68‑1 (MQ=255)                               tGCAGGAAGTGTTTGATGACATTAAGTTGTTTAGTCACGGAGGGTCGGGGCTGTAATTGCCACTTTTc                               <  1:284063/68‑1 (MQ=255)                               tGCAGGAAGTGTTTGATGACATTAAGTTGTTTAGTCACGGAGGGTCGGGGCTGTAATTGCCACTTTTc                               <  1:2283789/68‑1 (MQ=255)                               tGCAGGAAGTGTTTGATGACATTAAGTTGTTTAGTCACGGAGGGGCGGGGCTGTAATTGCCACTTTTc                               <  1:282294/68‑1 (MQ=255)                                 cAGGAACCGTTTGATGACGTTAAGTTGTTTGGTCACGGAGGGTCGGGGCTGTAATTGCCACTTTTCCgg                            >  1:989616/1‑69 (MQ=255)                                 cAGGAACCGTTTGATGACGTTAAGTTCTTTGGTCACGGAGGGTCGGGGCTGTAATTGCCACTTTTCCgg                            >  1:1804679/1‑69 (MQ=255)                                                      aaGTTGTTTGGTCACGGAGGGTCGGGGCTGTAATTGCCACTTTTCCGGGACAATCGGCAAATCCGCAAg       >  1:3357615/1‑69 (MQ=255)                                                      aaGTTGTTTGGTCACGGAGGGTCGGGGCTGTAATTGCCACTTTTCCGGGACAATCGGCAAATCCGCAAg       >  1:2409644/1‑69 (MQ=255)                                                          tgttTGGTCACGGAGGGTCGGGGCTGTAATTGCCACTTTTCCGGGATAATCGGCAAATCCGCAAGAt     >  1:1341465/1‑67 (MQ=255)                                                           gttTGGTCACGGAGGGTCGGGGCTGTAATTGCCACTTTTCCGGGACAATCGGCAAATCCGCAAGATTcc  >  1:2045738/1‑69 (MQ=255)                                                           gttTGGTCACGGAGGGTCGGGGCTGTAATTGCCACTTTTCCGGGACAATCGGCAAATCCGCAAGATTcc  >  1:1368329/1‑69 (MQ=255)                                                               |                                                                 TCCCCGGCGTGAACGATTTCGCTGGCTTCATGCAGGAACCGTTTGATGACGTTAAGTTGTTTGGTCACGGAGGGTCGGGGCTGTAATTGCCACTTTTCCGGGACAATCGGCAAATCCGCAAGATTCC  >  minE/1174344‑1174470

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑