| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 96,648 | G→C | 100% | T231S (ACT→AGT) | yacF ← | conserved hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 96,648 | 0 | G | C | 100.0% | 69.6 / NA | 29 | T231S (ACT→AGT) | yacF | conserved hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base G (0/0); new base C (29/0); total (29/0) | |||||||||||
| Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
CAGTTCGAAATCCAGACGTTCCGGTACCTGTCCGTTTTCAGTGTCCAGCGGCATAAAACGAATGGCAAAA > minE/96608‑96677 | cAGTTCGAAATCCAGACGTTCTGGTACCTGTCCGTTTTCACTGTCCAGCGGCATAAAACGAATGGGaaa > 1:593225/1‑69 (MQ=255)cAGTTCGAAATCCAGACGTTCTGGTACCTGTCCGTTTTCACTGTCCAGCGGCATAAAACGAATGGCaaa > 1:1994847/1‑69 (MQ=255)cAGTTCGAAATCCAGACGTTCTGGTACCTGTCCGTTTTCACTGTCCAGCGGCATAAAACGAATGGCaaa > 1:2811762/1‑69 (MQ=255)cAGTTCGAAATCCAGACGTTCTGGTACCTGTCCGTTTTCACTGTCCAGCGGCATAAAACGAATGGCaaa > 1:2566303/1‑69 (MQ=255)cAGTTCGAAATCCAGACGTTCTGGTACCTGTCCGTTTTCACTGTCCAGCGGCATAAAACGAATGGCaaa > 1:2477692/1‑69 (MQ=255)cAGTTCGAAATCCAGACGTTCTGGTACCTGTCCGTTTTCACTGTCCAGCGGCATAAAACGAATGGCaaa > 1:3017058/1‑69 (MQ=255)cAGTTCGAAATCCAGACGTTCTGGTACCTGTCCGTTTTCACTGTCCAGCGGCATAAAACGAATGGCaaa > 1:3352220/1‑69 (MQ=255)cAGTTCGAAATCCAGACGTTCTGGTACCTGTCCGTTTTCACTGTCCAGCGGCATAAAACGAATGGCaaa > 1:2114372/1‑69 (MQ=255)cAGTTCGAAATCCAGACGTTCTGGTACCTGTCCGTTTTCACTGTCCAGCGGCATAAAACGAATGGCaaa > 1:409522/1‑69 (MQ=255)cAGTTCGAAATCCAGACGTTCTGGTACCTGTCCGTTTTCACTGTCCAGCGGCATAAAACGAATGGCaaa > 1:1627587/1‑69 (MQ=255)cAGTTCGAAATCCAGACGTTCTGGTACCTGTCCGTTTTCACTGTCCAGCGGCATAAAACGAATGGCaaa > 1:1575641/1‑69 (MQ=255)cAGTTCGAAATCCAGACGTTCTGGTACCTGTCCGTTTTCACTGTCCAGCGGCATAAAACGAATGGCaaa > 1:1495104/1‑69 (MQ=255)cAGTTCGAAATCCAGACGTTCTGGTACCTGTCCGTTTTCACTGTCCAGCGGCATAAAACGAATGGCaaa > 1:1290548/1‑69 (MQ=255)cAGTTCGAAATCCAGACGTTCTGGTACCTGTCCGTTTTCACTGTCCAGCGGCATAAAACGAATGGCaaa > 1:1278684/1‑69 (MQ=255)cAGTTCGAAATCCAGACGTTCTGGTACCTGTCCGTTTTCACTGTCCAGCGGCATAAAACGAATGGCaaa > 1:1060800/1‑69 (MQ=255)cAGTTCGAAATCCAGACGTTCTGGTACCTGTCCGTTTTCACTGTCCAGCGGCATAAAACGAATGGCaa > 1:405150/1‑68 (MQ=255)cAGTTCGAAATCCAGACGTTCTGGTACCTGTCCGTTTTCACTGTCCAGCGGCATAAAACGAATGGCaa > 1:1001274/1‑68 (MQ=255) aGTTCGAAATCCAGACGTTCTTGTACCTGTCCGTTTTCACTGTCCAGCGGCATAAAACGAATGGCaaaa > 1:2445112/1‑69 (MQ=255) aGTTCGAAATCCAGACGTTCTGGTACCTGTCCGTTTTCACTGTCCAGCGGCATAAAACGAATGGCaaaa > 1:2717120/1‑69 (MQ=255) aGTTCGAAATCCAGACGTTCTGGTACCTGTCCGTTTTCACTGTCCAGCGGCATAAAACGAATGGCaaaa > 1:25706/1‑69 (MQ=255) aGTTCGAAATCCAGACGTTCTGGTACCTGTCCGTTTTCACTGTCCAGCGGCATAAAACGAATGGCaaaa > 1:2230702/1‑69 (MQ=255) aGTTCGAAATCCAGACGTTCTGGTACCTGTCCGTTTTCACTGTCCAGCGGCATAAAACGAATGGCaaaa > 1:3061779/1‑69 (MQ=255) aGTTCGAAATCCAGACGTTCTGGTACCTGTCCGTTTTCACTGTCCAGCGGCATAAAACGAATGGCaaaa > 1:3321179/1‑69 (MQ=255) aGTTCGAAATCCAGACGTTCTGGTACCTGTCCGTTTTCACTGTCCAGCGGCATAAAACGAATGGCaaaa > 1:2214083/1‑69 (MQ=255) aGTTCGAAATCCAGACGTTCTGGTACCTGTCCGTTTTCACTGTCCAGCGGCATAAAACGAATGGCaaaa > 1:3422043/1‑69 (MQ=255) aGTTCGAAATCCAGACGTTCTGGTACCTGTCCGTTTTCACTGTCCAGCGGCATAAAACGAATGGCaaaa > 1:2064336/1‑69 (MQ=255) aGTTCGAAATCCAGACGTTCTGGTACCTGTCCGTTTTCACTGTCCAGCGGCATAAAACGAATGGCaaaa > 1:1890583/1‑69 (MQ=255) aGTTCGAAATCCAGACGTTCTGGTACCTGTCCGTTTTCACTGTCCAGCGGCATAAAACGAATGGCaaaa > 1:1692995/1‑69 (MQ=255) aGTTCGAAATCCAGACGTTCTGGTACCTGTCCGTTTTCACTGTCCAGAGGCATAAAACGAATGGCaaaa > 1:613682/1‑69 (MQ=255) | CAGTTCGAAATCCAGACGTTCCGGTACCTGTCCGTTTTCAGTGTCCAGCGGCATAAAACGAATGGCAAAA > minE/96608‑96677 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |