| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,178,823 | C→A | 45.7% | T91T (ACC→ACA) | ansA → | cytoplasmic L‑asparaginase I |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,178,823 | 0 | C | A | 45.7% | 33.4 / 95.7 | 94 | T91T (ACC→ACA) | ansA | cytoplasmic L‑asparaginase I |
| Reads supporting (aligned to +/- strand): ref base C (21/30); new base A (19/24); total (40/54) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 8.36e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.68e-01 | |||||||||||
GATATTAAAGCGCACTATGACGACTATGATGGTTTTGTCATTCTGCACGGCACCGACACGATGGCGTATACCGCCTCTGCGCTGTCGTTCATGCTCGAGAATCTCGGTAAACCGGTCAT > minE/1178770‑1178888 | gATATTAAAGCGCACTATGACGACTATGATGGTTTTGTCATTCTGCACGGCACCGACACGATGGCGtat > 1:2250596/1‑69 (MQ=255)gATATTAAAGCGCACTATGACGACTATGATGGTTTTGTCATTCTGCACGGCACCGACACGATGGCGtat > 1:2333695/1‑69 (MQ=255)gATATTAAAGCGCACTATGACGACTATGATGGTTTTGTCATTCTGCACGGCACCGACACGATGGCGtat > 1:2413705/1‑69 (MQ=255)gATATTAAAGCGCACTATGACGACTATGATGGTTTTGTCATTCTGCACGGCACCGACACGATGGCGtat > 1:2520734/1‑69 (MQ=255)gATATTAAAGCGCACTATGACGACTATGATGGTTTTGTCATTCTGCACGGCACCGACACGATGGCGtat > 1:3052050/1‑69 (MQ=255)gATATTAAAGCGCACTATGACGACTATGATGGTTTTGTCATTCTGCACGGCACCGACACGATGGCGtat > 1:440705/1‑69 (MQ=255)gATATTAAAGCGCACTATGACGACTATGATGGTTTTGTCATTCTGCACGGCACCGACACGATGGCGtat > 1:3366166/1‑69 (MQ=255) aaaGCGCACTATGACGACTATGATGGTTTTGTCATTCTGCACGGCACCGACACGATGGCGTATACCGc < 1:2917763/68‑1 (MQ=255) aaaGCGCACTATGACGACTATGATGGTTTTGTCATTCTGCACGGCACCGACACGATGGCGTATACCGc < 1:1299201/68‑1 (MQ=255) aaaGCGCACTATGACGACTATGATGGTTTTGTCATTCTGCACGGCACCGACACGATGGCGTATACCGc < 1:134140/68‑1 (MQ=255) aaaGCGCACTATGACGACTATGATGGTTTTGTCATTCTGCACGGCACCGACACGATGGCGTATACCGc < 1:2030140/68‑1 (MQ=255) aaaGCGCACTATGACGACTATGATGGTTTTGTCATTCTGCACGGCACCGACACGATGGCGTATACCGc < 1:351305/68‑1 (MQ=255) aaaGCGCACTATGACGACTATGATGGTTTTGTCATTCTGCACGGCACCGACACGATGGCGTATACCGc < 1:2100216/68‑1 (MQ=255) aTGACGACTATGATGGTTTTGTCATTCTGCACGGCACCGacac > 1:1504711/1‑43 (MQ=255) aTGACGACTATGATGGTTTTGTCATTCTGCACGGCACCGacac > 1:1508569/1‑43 (MQ=255) aTGACGACTATGATGGTTTTGTCATTCTGCACGGCACCGacac > 1:621147/1‑43 (MQ=255) aTGACGACTATGATGGTTTTGTCATTCTGCACGGCACCGacac > 1:10720/1‑43 (MQ=255) aTGACGACTATGATGGTTTTGTCATTCTGCACGGCACCGACACGATGGCGTATACCGCCTCTGCGCt > 1:215505/1‑67 (MQ=255) aTGACGACTATGATGGTTTTGTCATTCTGCACGGCACCGACACGATGGCGTATACCGCCTCTGCGCTGt > 1:2486516/1‑69 (MQ=255) tATGATGGTTTTGTCATTCTGCACGGCACCGacac > 1:1752567/1‑35 (MQ=255) tATGATGGTTTTGTCATTCTGCACGGCACCGacac > 1:2443668/1‑35 (MQ=255) tATGATGGTTTTGTCATTCTGCACGGCACCGacac > 1:2663560/1‑35 (MQ=255) tATGATGGTTTTGTCATTCTGCACGGCACCGacac > 1:2667456/1‑35 (MQ=255) tATGATGGTTTTGTCATTCTGCACGGCACCGacac > 1:1832364/1‑35 (MQ=255) tATGATGGTTTTGTCATTCTGCACGGCACCGacac > 1:3103171/1‑35 (MQ=255) tATGATGGTTTTGTCATTCTGCACGGCACCGacac > 1:960295/1‑35 (MQ=255) atgGTTTTGTCATTCTGCACGGCACCGACACGATGGCGTATACCGCCTCTGCGCTGTCGt < 1:2973523/60‑1 (MQ=255) atgGTTTTGTCATTCTGCACGGCACCGACACGATGGCGTATACCGCCTCTGCGCTGTCGt < 1:2634533/60‑1 (MQ=255) atgGTTTTGTCATTCTGCACGGCACCGACACGATGGCGTATACCGCCTCTGCGCTGTCGt < 1:2036724/60‑1 (MQ=255) atgGTTTTGTCATTCTGCACGGCACCGACACGATGGCGTATACCGCCTCTGCGCTGTCGt < 1:1639198/60‑1 (MQ=255) atgGTTTTGTCATTCTGCACGGCACCGACACGATGGCGTATACCGCCTCTGCGCTGTCGt < 1:1435850/60‑1 (MQ=255) atgGTTTTGTCATTCTGCACGGCACCGACACGATGGCGTATACCGCCTCTGCGCTGTCGt < 1:557825/60‑1 (MQ=255) atgGTTTTGTCATTCTGCACGGCACCGACACGATGGCGTATACCGCCTCTGCGCTGTCGt < 1:2257998/60‑1 (MQ=255) tttGTCATTCTGCACGGCACCGACACGATGGCGTATACCGCCTCTGCGCTGTCGTTCATGCTCGAGaa < 1:2758563/68‑1 (MQ=255) tttGTCATTCTGCACGGCACCGACACGATGGCGTATACCGCCTCTGCGCTGTCGTTCATGCTCGAGaa < 1:993890/68‑1 (MQ=255) tttGTCATTCTGCACGGCACCGACACGATGGCGTATACCGCCTCTGCGCTGTCGTTCATGCTCGAGaa < 1:3395173/68‑1 (MQ=255) tttGTCATTCTGCACGGCACCGACACGATGGCGTATACCGCCTCTGCGCTGTCGTTCATGCTCGAGaa < 1:637998/68‑1 (MQ=255) tttGTCATTCTGCACGGCACCGACACGATGGCGTATACCGCCTCTGCGCTGTCGTTCATGCTCGAGaa < 1:850055/68‑1 (MQ=255) tttGTCATTCTGCACGGCACCGACACGATGGCGTATACCGCCTCTGCGCTGTCGTTCATGCTCGAGaa < 1:230257/68‑1 (MQ=255) tttGTCATTCTGCACGGCACCGACACGATGGCGTATACCGCCTCTGCGCTGTCGTTCATGCTCGAGaa < 1:1176718/68‑1 (MQ=255) tttGTCATTCTGCACGGCACCGACACGATGGCGTATACCGCCTCTGCGCTGTCGTTCATGCTCGAGaa < 1:2028356/68‑1 (MQ=255) ttGTCATTCTGCACGGTACAGACACGATGGCGTATACCGCCTCTGCACTGTCGTTCATGCTCGAGAAtc > 1:3141321/1‑69 (MQ=255) ttGTCATTCTGCACGGTACAGACACGATGGCGTATACCGCCTCTGCACTGTCGTTCATGCTCGAGAAtc > 1:1619155/1‑69 (MQ=255) ttGTCATTCTGCACGGTACAGACACGATGGCGTATACCGCCTCTGCACTGTCGTTCATGCTCGAGAAtc > 1:1498042/1‑69 (MQ=255) ttGTCATTCTGCACGGTACAGACACGATGGCGTATACCGCCTCTGCACTGTCGTTCATGCTCGAGAAtc > 1:495347/1‑69 (MQ=255) ttGTCATTCTGCACGGTACAGACACGATGGCGTATACCGCCTCTGCACTGTCGTTCATGCTCGAGAAtc > 1:1948663/1‑69 (MQ=255) ttGTCATTCTGCACGGTACAGACACGATGGCGTATACCGCCTCTGCACTGTCGTTCATGCTCGAGAAtc > 1:1353197/1‑69 (MQ=255) ttGTCATTCTGCACGGTACAGACACGATGGCGTATACCGCCTCTGCACTGTCGTTCATGCTCGAGAAtc > 1:1963260/1‑69 (MQ=255) ttGTCATTCTGCACGGTACAGACACGATGGCGTATACCGCCTCTGCACTGTCGTTCATGCTCGAGAAtc > 1:120273/1‑69 (MQ=255) ttGTCATTCTGCACGGTACAGACACGATGGCGTATACCGCCTCTGCACTGTCGTTCATGCTCGAGAAtc > 1:2559906/1‑69 (MQ=255) ttGTCATTCTGCACGGTACAGACACGATGGCGTATACCGCCTCTGCACTGTCGTTCATGCTCGAGAAtc > 1:804464/1‑69 (MQ=255) ttGTCATTCTGCACGGTACAGACACGATGGCGTATACCGCCTCTGCACTGTCGTTCATGCTCGAGAAtc > 1:2483896/1‑69 (MQ=255) ttGTCATTCTGCACGGTACAGACACGATGGCGTATACCGCCTCTGCACTGTCGTTCATGCTCGAGAAtc > 1:819074/1‑69 (MQ=255) ttGTCATTCTGCACGGTACAGACACGATGGCGTATACCGCCTCTGCACTGTCGTTCATGCTCGAGAAtc > 1:243729/1‑69 (MQ=255) ttGTCATTCTGCACGGTACAGACACGATGGCGTATACCGCCTCTGCACTGTCGTTCATGCTCGAGAAtc > 1:838764/1‑69 (MQ=255) ttGTCATTCTGCACGGTACAGACACGATGGCGTATACCGCCTCTGCACTGTCGTTCATGCTCGAGAAt > 1:2197769/1‑68 (MQ=255) ttGTCATTCTGCACGGTACAGACACGATGGCGTATACCGCCTCTGAACTGTCGTTCATGCTCGAGAAt > 1:3285111/1‑68 (MQ=255) ttGTCATTCTGCACGGTACAGACACGATGGCGTATACCGCATCTGCACTGTCGTTCATGCTCGAGAAtc > 1:2070818/1‑69 (MQ=255) ttGTCATTATGCACGGTACAGACACGATGGCGTATACCGCCTCTGCACTGTCGTTCATGCTCGAGaa > 1:2565139/1‑67 (MQ=255) atGTCATTCTGCACGGTACAGACACGATGGCGTATACCGCCTCTGCACTGTCGTTCATGc > 1:2305838/2‑60 (MQ=255) cATTCTGCACGGCACCGACACGATGGCGTATACCGCCTCTGCGCTGTCGt < 1:173979/50‑1 (MQ=255) cGGTACAGACACGATGGCGTATACCGCCTCTGCACTGTCGTTCATGCTCGAGAATCTCGGTAAACCg < 1:351666/67‑1 (MQ=255) cGGTACAGACACGATGGCGTATACCGCCTCTGCACTGTCGTTCATGCTCGAGAATCTCGGTAAACCg < 1:2629084/67‑1 (MQ=255) cGGTACAGACACGATGGCGTATACCGCCTCTGCACTGTCGTTCATGCTCGAGAATCTCGGTAAACCg < 1:248783/67‑1 (MQ=255) cGGTACAGACACGATGGCGTATACCGCCTCTGCACTGTCGTTCATGCTCGAGAATCTCGGTAAACCg < 1:605519/67‑1 (MQ=255) cGGTACAGACACGATGGCGTATACCGCCTCTGCACTGTCGTTCATGCTCGAGAATCTCGGTAAACCg < 1:2645369/67‑1 (MQ=255) cGGTACAGACACGATGGCGTATACCGCCTCTGCACTGTCGTTCATGCTCGAGAATCTCGGTAAACCg < 1:541920/67‑1 (MQ=255) cGGTACAGACACGATGGCGTATACCGCCTCTGCACTGTCGTTCATGCTCGAGAATCTCGGTAAACCg < 1:531040/67‑1 (MQ=255) cGGTACAGACACGATGGCGTATACCGCCTCTGCACTGTCGTTCATGCTCGAGAATCTCGGTAAACCg < 1:514743/67‑1 (MQ=255) cGGTACAGACACGATGGCGTATACCGCCTCTGCACTGTCGTTCATGCTCGAGAATCTCGGTAAACCg < 1:502669/67‑1 (MQ=255) cGGTACAGACACGATGGCGTATACCGCCTCTGCACTGTCGTTCATGCTCGAGAATCTCGGTAAACCg < 1:2169870/67‑1 (MQ=255) cGGTACAGACACGATGGCGTATACCGCCTCTGCACTGTCGTTCATGCTCGAGAATCTCGGTAAACCg < 1:395790/67‑1 (MQ=255) cGGTACAGACACGATGGCGTATACCGCCTCTGCACTGTCGTTCATGCTCGAGAATCTCGGTAAACCg < 1:2190353/67‑1 (MQ=255) cGGTACAGACACGATGGCGTATACCGCCTCTGCACTGTCGTTCATGCTCGAGAATCTCGGTAAACCg < 1:353679/67‑1 (MQ=255) cGGTACAGACACGATGGCGTATACCGCCTCTGCACTGTCGTTCATGCTCGAGAATCTCGGTAAACCg < 1:236701/67‑1 (MQ=255) cGGTACAGACACGATGGCGTATACCGCCTCTGCACTGTCGTTCATGCTCGAGAATCTCGGTAAACCg < 1:157458/67‑1 (MQ=255) cGGTACAGACACGATGGCGTATACCGCCTCTGCACTGTCGTTCATGCTCGAGAATCTCGGTAAACCg < 1:3379424/67‑1 (MQ=255) cGGTACAGACACGATGGCGTATACCGCCTCTGCACTGTCGTTCATGCTCGAGAATCTCGGTAAACCg < 1:1919565/67‑1 (MQ=255) cGGTACAGACACGATGGCGTATACCGCCTCTGCACTGTCGTTCATGCTCGAGAATCTCGGTAAACCg < 1:3362331/67‑1 (MQ=255) cGGTACAGACACGATGGCGTATACCGCCTCTGCACTGTCGTTCATGCTCGAGAATCTCGGTAAACCg < 1:899626/67‑1 (MQ=255) cGGTACAGACACGATGGCGTATACCGCCTCTGCACTGTCGTTCATGCTCGAGAATCTCGGTAAACCg < 1:1072775/67‑1 (MQ=255) cGGTACAGACACGATGGCGTATACCGCCTCTGCACTGTCGTTCATGCTCGAGAATCTCGGTAAACCg < 1:1883401/67‑1 (MQ=255) cGGTACAGACACGATGGCGTATACCGCCTCTGCACTGTCGTTCATGCTCGAGAATCTCGGTAAACCg < 1:1727794/67‑1 (MQ=255) cGGTACAGACACGATGGCGTATACCGCCTCTGCACTGTCGTTCATGCTCGAGAATCTCGGTAAACCg < 1:3050909/67‑1 (MQ=255) cGGTACAGACACGATGGCGTATACCGCCTCTGCACTGTCGTTCATGCTCGAGAATCTCGGTAAACCg < 1:105127/67‑1 (MQ=255) cGGCACCGACACGATGGCGTATACCGCCTCTGCGCTGTCGTTCATGCTCGAGAATCTCGGTAAACCGGt > 1:1936082/1‑69 (MQ=255) cACCGACACGATGGCGTATACCGCCTCTGCGCTGTCGTTCATGCTCGAGAATCTCGGTAAACCGGTCAt < 1:1845956/69‑1 (MQ=255) cACCGACACGATGGCGTATACCGCCTCTGCGCTGTCGTTCATGCTCGAGAATCTCGGTAAACCGGTCAt < 1:1267678/69‑1 (MQ=255) cACCGACACGATGGCGTATACCGCCTCTGCGCTGTCGTTCATGCTCGAGAATCTCGGTAAACCGGTCAt < 1:369061/69‑1 (MQ=255) cACCGACACGATGGCGTATACCGCCTCTGCGCTGTCGTTCATGCTCGAGAATCTCGGTAAACCGGTCAt < 1:1505281/69‑1 (MQ=255) cACCGACACGATGGCGTATACCGCCTCTGCGCTGTCGTTCATGCTCGAGAATCTCGGTAAACCGGTCAt < 1:3310876/69‑1 (MQ=255) cACCGACACGATGGCGTATACCGCCTCTGCGCTGTCGTTCATGCTCGAGAATCTCGGTAAACCGGTCAt < 1:2767495/69‑1 (MQ=255) cACCGACACGATGGCGTATACCGCCTCTGCGCTGTCGTTCATGCTCGAGAATCTCGGTAAACCGGTCAt < 1:2949516/69‑1 (MQ=255) aCCGACACGATGGCGTATACCGCCTCTGCGCTGTCGTTCATGCTCGAGAATCTCGGTAAACCGGTCAt < 1:269187/68‑1 (MQ=255) | GATATTAAAGCGCACTATGACGACTATGATGGTTTTGTCATTCTGCACGGCACCGACACGATGGCGTATACCGCCTCTGCGCTGTCGTTCATGCTCGAGAATCTCGGTAAACCGGTCAT > minE/1178770‑1178888 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |