| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,178,979 | G→T | 32.8% | P143P (CCG→CCT) | ansA → | cytoplasmic L‑asparaginase I |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,178,979 | 0 | G | T | 32.8% | 84.9 / 46.6 | 73 | P143P (CCG→CCT) | ansA | cytoplasmic L‑asparaginase I |
| Reads supporting (aligned to +/- strand): ref base G (26/23); new base T (21/3); total (47/26) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.25e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
TGAGTTACGCTCTGACGGACAAATTAATCTGCTGAATGCGTTGTACGTTGCGGCGAATTATCCGATCAACGAAGTAACGCTCTTTTTCAATAACCGATTGTATCGCGGCAACCGCACTACCAAAGCCCATG > minE/1178916‑1179046 | tgAGTTACGCTCTGACGGACAAATTAATCTGCTGAATGCGTTGTACGTTGCGGCGAATTATCCGATc < 1:1121446/67‑1 (MQ=255)tgAGTTACGCTCTGACGGACAAATTAATCTGCTGAATGCGTTGTACGTTGCGGCGAATTATCCGATc < 1:311049/67‑1 (MQ=255)tgAGTTACGCTCTGACGGACAAATTAATCTGCTGAATGCGTTGTACGTTGCGGCGAATTATCCGATc < 1:125922/67‑1 (MQ=255)tgAGTTACGCTCTGACGGACAAATTAATCTGCTGAATGCGTTGTACGTTGCGGCGAATTATCCGATc < 1:2186482/67‑1 (MQ=255)tgAGTTACGCTCTGACGGACAAATTAATCTGCTGAATGCGTTGTACGTTGCGGCGAATTATCCGATc < 1:1611877/67‑1 (MQ=255)tgAGTTACGCTCTGACGGACAAATTAATCTGCTGAATGCGTTGTACGTTGCGGCGAATTATCCGATc < 1:145727/67‑1 (MQ=255) tACGCTCTGACGGACAAATTAATCTGCTGAATGCGTTGTATGTTGCGGCGAATTATCCGATCAACGAAg < 1:1252151/69‑1 (MQ=255) tACGCTCTGACGGACAAATTAATCTGCTGAATGCGTTGTACGTTGCGGCGAATTATCCGATCAACGAAg < 1:1399084/69‑1 (MQ=255) tACGCTCTGACGGACAAATTAATCTGCTGAATGCGTTGTACGTTGCGGCGAATTATCCGATCAACGAAg < 1:846233/69‑1 (MQ=255) aCGCTCTGACGGACAAATTAATCTGCTGAATGCGTTGTACGTTGCGGCGAATTATCCGATCAACGa < 1:87479/66‑1 (MQ=255) aCGCTCTGACGGACAAATTAATCTGCTGAATGCGTTGTACGTTGCGGCGAATTATCCGATCAACGa < 1:1373964/66‑1 (MQ=255) ggACAAATTAATCTGCTGAATGCGTTGTACGTTGCGGCGAATTATCCGTTCAACGa < 1:1254928/56‑1 (MQ=255) gACAAATTAATCTGCTGAATGCGTTGTACGTTGCGGCGAATTATCCTATCAACGAAGTAACTCTCttt > 1:439521/1‑68 (MQ=255) gACAAATTAATCTGCTGAATGCGTTGTACGTTGCGGCGAATTATCCTATCAACGAAGTAACTCTCttt > 1:3386954/1‑68 (MQ=255) gACAAATTAATCTGCTGAATGCGTTGTACGTTGCGGCGAATTATCCTATCAACGAAGTAACTCTCttt > 1:3222790/1‑68 (MQ=255) gACAAATTAATCTGCTGAATGCGTTGTACGTTGCGGCGAATTATCCTATCAACGAAGTAACTCTCttt > 1:427031/1‑68 (MQ=255) gACAAATTAATCTGCTGAATGCGTTGTACGTTGCGGCGAATTATCCTATCAACGAAGTAACTCTCttt > 1:2031449/1‑68 (MQ=255) gACAAATTAATCTGCTGAATGCGTTGTACGTTGCGGCGAATTATCCTATCAACGAAGTAACTCTCttt > 1:3404197/1‑68 (MQ=255) gACAAATTAATCTGCTGAATGCGTTGTACGTTGCGGCGAATTATCCTATCAACGAAGTAACTCTCttt > 1:3389227/1‑68 (MQ=255) gACAAATTAATCTGCTGAATGCGTTGTACGTTGCGGCGAATTATCCTATCAACGAAGTAACTCTCttt > 1:220592/1‑68 (MQ=255) gACAAATTAATCTGCTGAATGCGTTGTACGTTGCGGCGAATTATCCTATCAACGAAGTAACTCTCttt > 1:2253287/1‑68 (MQ=255) gACAAATTAATCTGCTGAATGCGTTGTACGTTGCGGCGAATTATCCTATCAACGAAGTAACTCTCttt > 1:3012755/1‑68 (MQ=255) gACAAATTAATCTGCTGAATGCGTTGTACGTTGCGGCGAATTATCCTATCAACGAAGTAACTCTCttt > 1:505302/1‑68 (MQ=255) gACAAATTAATCTGCTGAATGCGTTGTACGTTGCGGCGAATTATCCTATCAACGAAGTAACTCTCttt > 1:2709760/1‑68 (MQ=255) gACAAATTAATCTGCTGAATGCGTTGTACGTTGCGGCGAATTATCCTATCAACGAAGTAACTCTCttt > 1:613071/1‑68 (MQ=255) gACAAATTAATCTGCTGAATGCGTTGTACGTTGCGGCGAATTATCCTATCAACGAAGTAACTCTCttt > 1:156604/1‑68 (MQ=255) gACAAATTAATCTGCTGAATGCGTTGTACGTTGCGGCGAATTATCCTATCAACGAAGTAACTCTCttt > 1:1436440/1‑68 (MQ=255) gACAAATTAATCTGCTGAATGCGTTGTACGTTGCGGCGAATTATCCTATCAACGAAGTAACTCTCttt > 1:1337122/1‑68 (MQ=255) gACAAATTAATCTGCTGAATGCGTTGTACGTTGCGGCGAATTATCCTATCAACGAAGTAACTCTCttt > 1:1296587/1‑68 (MQ=255) gACAAATTAATCTGCTGAATGCGTTGTACGTTGCGGCGAATTATCCTATCAACGAAGTAACTCTCttt > 1:93790/1‑68 (MQ=255) gACAAATTAATCTGCTGAATGCGTTGTACGTTGCGGCGAATTATCCTATCAACGAAGTAACTCTCttt > 1:1033717/1‑68 (MQ=255) gACAAATTAATCTGCTGAATGCGTTGTACGTTGCGGCGAATTATCCTATCAACGAAGAAActct > 1:2081516/1‑64 (MQ=255) gACAAATTAATCTGATGAATGCGTTGTACGTTGCGGCGAATTATCCTATCAACGAAGTAACTCTCttt > 1:2386042/1‑68 (MQ=255) cAAATTAATCTGCTGAATGCGTTGTTCGTTGCGGCGAATTATCCGATCAACGAAGTAACGCTCTTTTTc > 1:2380877/1‑69 (MQ=255) cAAATTAATCTGCTGAATGCGTTGTACGTTGCGGCGAATTATCCGATCAACGAAGTAACGCTCTTTTTc > 1:1959763/1‑69 (MQ=255) cAAATTAATCTGCTGAATGCGTTGTACGTTGCGGCGAATTATCCGATCAACGAAGTAACGCTCTTTTTc > 1:2304872/1‑69 (MQ=255) cAAATTAATCTGCTGAATGCGTTGTACGTTGCGGCGAATTATCCGATCAACGAAGTAACGCTCTTTTTc > 1:362395/1‑69 (MQ=255) cAAATTAATCTGCTGAATGCGTTGTACGTTGCGGCGAATTATCCGATCAACGAAGTAACGCTCTTTTTc > 1:499694/1‑69 (MQ=255) cAAATTAATCTGCTGAATGCGTTGTACGTTGCGGCGAATTATCCGATCAACGAAGTAACGCTCTTTTTc > 1:571245/1‑69 (MQ=255) tGCGTTGTACGTTGCGGCGAATTATCCGATCAACGAAGTAAc > 1:1883083/1‑42 (MQ=255) tGCGTTGTACGTTGCGGCGAATTATCCGATCAACGAAGTAACGCTCTTTTTCAATAACCGATTGTATcg > 1:3423746/1‑69 (MQ=255) tGCGTTGTACGTTGCGGCGAATTATCCGATCAACGAAGTAACGCTCTTTTTCAATAACCGATTGTATcg > 1:3259703/1‑69 (MQ=255) tGCGTTGTACGTTGCGGCGAATTATCCGATCAACGAAGTAACGCTCTTTTTCAATAACCGATTGTATcg > 1:988584/1‑69 (MQ=255) tGCGTTGTACGTTGCGGCGAATTATCCGATCAACGAAGTAACGCTCTTTTTCAATAACCGATTGTATcg > 1:3020147/1‑69 (MQ=255) tGCGTTGTACGTTGCGGCGAATTATCCGATCAACGAAGTAACGCTCTTTTTCAATAACCGATTGTATcg > 1:2921950/1‑69 (MQ=255) tGCGTTGTACGTTGCGGCGAATTATCCGATCAACGAAGTAACGCTCTTTTTCAATAACCGATTGTATcg > 1:2894040/1‑69 (MQ=255) tGCGTTGTACGTTGCGGCGAATTATCCGATCAACGAAGTAACGCTCTTTTTCAATAACCGATTGTATcg > 1:2624629/1‑69 (MQ=255) tGCGTTGTACGTTGCGGCGAATTATCCGATCAACGAAGTAACGCTCTTTTTCAATAACCGATTGTATcg > 1:1037031/1‑69 (MQ=255) tGCGTTGTACGTTGCGGCGAATTATCCGATCAACGAAGTAACGCTCTTTTTCAATAACCGATTGTATcg > 1:1519654/1‑69 (MQ=255) tGCGTTGTACGTTGCGGCGAATTATCCGATCAACGAAGTAACGCTCTTTTTCAATAACCGATTGTATcg > 1:1578024/1‑69 (MQ=255) tGCGTTGTACGTTGCGGCGAATTATCCGATCAACGAAGTAACGCTCTTTTTCAATAACCGATTGTATcg > 1:1664667/1‑69 (MQ=255) tGCGTTGTACGTTGCGGCGAATTATCCGATCAACGAAGTAACGCTCTTTTTCAATAACCGATTGTATcg > 1:1794697/1‑69 (MQ=255) tGCGTTGTACGTTGCGGCGAATTATCCGATCAACGAAGTAACGCTCTTTTTCAATAACCGATTGTATcg > 1:2313797/1‑69 (MQ=255) tGCGTTGTACGTTGCGGCGAATTATCCGATCAACGAAGTAACGCTCTTTTTCAATAACCGATTGTATcg > 1:2609692/1‑69 (MQ=255) tGCGTTGTACGTTGCGGCGAATTATCCGATCAACGAAGTAACGCTCTTTTTCAATAACCGATTGTATcg > 1:2526320/1‑69 (MQ=255) ttGCGGCGAATTATCCGATCAACGAAGTAACGCTCTTTTTCAATAACCGATTGTATCGCGGCAACCGc < 1:2710023/68‑1 (MQ=255) ttGCGGCGAATTATCCGATCAACGAAGTAACGCTCTTTTTCAATAACCGATTGTATCGCGGCAACCGc < 1:2076719/68‑1 (MQ=255) ttGCGGCGAATTATCCGATCAACGAAGTAACGCTCTTTTTCAATAACCGATTGTATCGCGGCAACCGc < 1:3410863/68‑1 (MQ=255) ttGCGGCGAATTATCCGATCAACGAAGTAACGCTCTTTTTCAATAACCGATTGTATCGCGGCAACCGc < 1:3431809/68‑1 (MQ=255) ttGCGGCGAATTATCCGATCAACGAAGTAACGCTCTTTTTCAATAACCGATTGTATCGCGGCAACCGc < 1:1991410/68‑1 (MQ=255) ttGCGGCGAATTATCCGATCAACGAAGTAACGCTCTTTTTCAATAACCGATTGTATCGCGGCAACCGc < 1:3383709/68‑1 (MQ=255) gtGCGGCGAATTATCCGATCAACGAAGTAACGCTCTTTTTCAATAACCGATTGTATCGCGGCAACCGCa < 1:1175690/68‑1 (MQ=255) gAATTATCCGATCAACGAAGTAACGCTCTTTTTCAATAACCGATTGTATCGCGGCAACCGCACTACCaa > 1:2488199/1‑69 (MQ=255) tATCCTATCAACGAAGTAACTCTCTTTTTTAATAATCGACTGTATCGTGGCAACCGAACCACCAAAGca < 1:2005853/69‑2 (MQ=255) tATCCTATCAACGAAGTAACTCTCTTTTTTAATAATCGACTGTATCGTGGCAACCGAACCACCAAAGca < 1:418407/69‑2 (MQ=255) tATCCTATCAACGAAGTAACTCTCTTTTTTAATAATCGACTGTATCGTGGCAACCGAACCACCAAAGca < 1:2435683/69‑2 (MQ=255) tATCCGATCAACGAAGTAACGCTCTTTTTCAATAACCGATTGTATCGCGGCAACCGCACTACCAAAGcc > 1:1976982/1‑69 (MQ=255) tATCCGATCAACGAAGTAACGCTCTTTTTCAATAACCGATTGTATCGCGGCAACCGCACTACCAAAGcc > 1:1260539/1‑69 (MQ=255) tATCCGATCAACGAAGTAACGCTCTTTTTCAATAACCGATTGTATCGCGGCAACCGCACTACCAAAGcc < 1:1731508/69‑1 (MQ=255) tATCCGATCAACGAAGTAACGCTCTTTTTCAATAACCGATTGTATCGCGGCAACCGCACTACCAAAGcc < 1:3404969/69‑1 (MQ=255) tATCCGATCAACGAAGTAACGCTCTTTTTCAATAACCGATTGTATCGCGGCAACCGCACTACCAAAGcc < 1:2068130/69‑1 (MQ=255) tATCCGATCAACGAAGTAACGCTCTTTTTCAATAACCGATTGTATCGCGGCAACCGCACTACCAAAGcc < 1:2383754/69‑1 (MQ=255) cGATCAACGAAGTAACGCTCTTTTTCAATAACCGATTGTATCGCGGCAACCGCACTACCAAAGCCCATg > 1:1802168/1‑69 (MQ=255) | TGAGTTACGCTCTGACGGACAAATTAATCTGCTGAATGCGTTGTACGTTGCGGCGAATTATCCGATCAACGAAGTAACGCTCTTTTTCAATAACCGATTGTATCGCGGCAACCGCACTACCAAAGCCCATG > minE/1178916‑1179046 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |