| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,179,177 | A→G | 74.7% | P209P (CCA→CCG) | ansA → | cytoplasmic L‑asparaginase I |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,179,177 | 0 | A | G | 74.7% | 68.7 / 19.2 | 59 | P209P (CCA→CCG) | ansA | cytoplasmic L‑asparaginase I |
| Reads supporting (aligned to +/- strand): ref base A (10/5); new base G (26/18); total (36/23) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.62e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.49e-01 | |||||||||||
TTCGTCGTTTGAATACGCCACCCGCCCCGCACGGTGAAGGGGAATTAATCGTTCATCCAATCACCCCACAACCAATTGGCGTAGTGACGATTTATCCAGGGATTTCTGCTGACGTCGTGCGCAATTTT > minE/1179110‑1179237 | ttCGTCGTTTGAATACGCCACCCGCCCCGCTCGGTGAAGGGGAATTAATCGTTCATCCAATCACCCcac > 1:141285/1‑69 (MQ=255)ttCGTCGTTTGAATACGCCACCCGCCCCGCACGGTGAAGGGGAATTAATCGTTCATCCAATCACCCcac > 1:3331015/1‑69 (MQ=255)ttCGTCGTTTGAATACGCCACCCGCCCCGCACGGTGAAGGGGAATTAATCGTTCATCCAATCACCCcac > 1:207845/1‑69 (MQ=255)ttCGTCGTTTGAATACGCCACCCGCCCCGCACGGTGAAGGGGAATTAATCGTTCATCCAATCACCCcac > 1:2945645/1‑69 (MQ=255)ttCGTCGTTTGAATACGCCACCCGCCCCGCACGGTGAAGGGGAATTAATCGTTCATCCAATCACCCcac > 1:3085129/1‑69 (MQ=255)ttCGTCGTTTGAATACGCCACCCGCCCCGCACGGTGAAGGGGAATTAATCGTTCATCCAATCACCCcac > 1:2091867/1‑69 (MQ=255)ttCGTCGTTTGAATACGCCACCCGCCCCGCACGGTGAAGGGGAATTAATCGTTCATCCAATCACCCcac > 1:3095967/1‑69 (MQ=255)ttCGTCGTTTGAATACGCCACCCGCCCCGCACGGTGAAGGGGAATTAATCGTTCATCCAATCACCCcac > 1:2280430/1‑69 (MQ=255)ttCGTCGTTTGAATACGCCACCCGCCCCGCACGGTGAAGGGGAATTAATCGTTCATCCAATCACCCcac > 1:370440/1‑69 (MQ=255)ttCGTCGTTTGAATACGCCACCCGCCCCGCACGGTGAAGGGGAATTAATCGTTCATCCAATCACCCcac > 1:2750736/1‑69 (MQ=255)ttCGTCGTTTGAATACGCCACCCGCCCCGCACGGTGAAGGGGAATTAATCGTTCATCCAATCACCCcac > 1:597804/1‑69 (MQ=255)ttCGTCGTTTGAATACGCCACCCGCCCCGCACGGTGAAGGGGAATTAATCGTTCATCCAATCACCCcac > 1:610276/1‑69 (MQ=255)ttCGTCGTTTGAATACGCCACCCGCCCCGCACGGTGAAGGGGAATTAATCGTTCATCCAATCACCCcac > 1:694236/1‑69 (MQ=255)ttCGTCGTTTGAATACGCCACCCGCCCCGCACGGTGAAGGGGAATTAATCGTTCATCCAATCACCCcac > 1:1448115/1‑69 (MQ=255)ttCGTCGTTTGAATACGCCACCCGCCCCGCACGGTGAAGGGGAATTAATCGTTCATCCAATCACCCcac > 1:781801/1‑69 (MQ=255)ttCGTCGTTTGAATACGCCACCCGCCCCGCACGGTGAAGGGGAATTAATCGTTCATCCAATCACCCcac > 1:3422776/1‑69 (MQ=255)ttCGTCGTTTGAATACGCCACCCGCCCCGCACGGTGAAGGGGAATTAATCGTTCATCCAATCACCCcac > 1:1217556/1‑69 (MQ=255) cgtTTGAATACGCCACCCGCCCCGCACGGTGAAGGGGAATTAATCGTTCATCCAATCACCCCACAAcc < 1:3219146/68‑1 (MQ=255) tGAATACGCCACCCGCCCCGCACGGTGAAGGGGAATTAATCGTTCATCCAATCACCCCACAACCAATTg > 1:2749567/1‑69 (MQ=255) tGAATACGCCACCCGCCCCGCACGGTGAAGGGGAATTAATCGTTCATCCAATCACCCCACAACCAATTg > 1:581399/1‑69 (MQ=255) tGAATACGCCACCCGCCCCGCACGGTGAAGGGGAATTAATCGTTCATCCAATCACCCCACAACCAATTg > 1:771047/1‑69 (MQ=255) tGAATACGCCACCCGCCCCGCACGGTGAAGGGGAATTAATCGTTCATCCAATCACCCCACAACCAATTg > 1:3110864/1‑69 (MQ=255) tGAATACGCCACCCGCCCCGCACGGTGAAGGGGAATTAATCGTTCATCCAATCACCCCACAACCAATTg > 1:2176797/1‑69 (MQ=255) tGAATACGCCACCCGCCCCGCACGGTGAAGGGGAATTAATCGTTCATCCAATCACCCCACAACCAATTg > 1:426595/1‑69 (MQ=255) tGAATACGCCACCCGCCCCGCACGGTGAAGGGGAATTAATCGTTCATCCAATCACCCCACAACCAATTg > 1:3398590/1‑69 (MQ=255) tACGCCACCCGCCCCGCACGGTGAAGGGGAATTAATCGTTCATCCAATCACCCCACAACCAATTGGCGt < 1:2806031/69‑1 (MQ=255) aCCCGTCCCGCCCGGTGAAGGGGCATTGATCGTTCATCCAATCACCCCGCAACCAATTg > 1:1928423/1‑59 (MQ=255) aCCCGTCCCGCACGGTGAAGGGGCATTGATCGTTCATCCAATCACGCCGCAACCAATTGGCGTAGTGa > 1:3196625/1‑68 (MQ=255) aCCCGTCCCGCACGGTGAAGGGGCATTGATCGTTCATCCAATCACGCCGCAACCAATTGGCGTAGTGAc > 1:2392907/1‑69 (MQ=255) aCCCGTCCCGCACGGTGAAGGGGCATTGATCGTTCATCCAATCACGCCGCAACCAATTGGCGTAGTGAc > 1:2708675/1‑69 (MQ=255) aCCCGTCCCGCACGGTGAAGGGGCATTGATCGTTCATCCAATCACGCCGCAACCAATTGGCGTAGTGAc > 1:2697765/1‑69 (MQ=255) aCCCGTCCCGCACGGTGAAGGGGCATTGATCGTTCATCCAATCACGCCGCAACCAATTGGCGTAGTGAc > 1:2691434/1‑69 (MQ=255) aCCCGTCCCGCACGGTGAAGGGGCATTGATCGTTCATCCAATCACGCCGCAACCAATTGGCGTAGTGAc > 1:1332834/1‑69 (MQ=255) aCCCGTCCCGCACGGTGAAGGGGCATTGATCGTTCATCCAATCACGCCGCAACCAATTGGCGTAGTGAc > 1:2563275/1‑69 (MQ=255) aCCCGTCCCGCACGGTGAAGGGGCATTGATCGTTCATCCAATCACGCCGCAACCAATTGGCGTAGTGAc > 1:1507378/1‑69 (MQ=255) aCCCGTCCCGCACGGTGAAGGGGCATTGATCGTTCATCCAATCACGCCGCAACCAATTGGCGTAGTGAc > 1:2069469/1‑69 (MQ=255) aCCCGTCCCGCACGGTGAAGGGGCATTGATCGTTCATCCAATCACGCCGCAACCAATTGGCGTAGTGAc > 1:2758716/1‑69 (MQ=255) aCCCGTCCCGCACGGTGAAGGGGCATTGATCGTTCATCCAATCACGCCGCAACCAATTGGCGTAGTGAc > 1:3146297/1‑69 (MQ=255) aCCCGTCCCGCACGGTGAAGGGGCATTGATCGTTCATCCAATCACGCCGCAACCAATTGGCGTAGTGAc > 1:1852521/1‑69 (MQ=255) aCCCGTCCCGCACGGTGAAGGGGCATTGATCGTTCATCCAATCACGCCGCAACCAATTGGCGTAGTGAc > 1:210540/1‑69 (MQ=255) aCCCGTCCCGCACGGTGAAGGGGCATTGATCGTTCATCCAATCACGCCGCAACCAATTGGCGTAGTGAc > 1:2910662/1‑69 (MQ=255) cgccccgcACGGTGAAGGGGAATTAATCGTTCATCCAATCACCCCACAACCAATTGGCGTAGTGACGa > 1:353751/1‑68 (MQ=255) cgccccgcACGGTGAAGGGGAATTAATCGTTCATCCAATCACCCCACAACCAATTGGCGTAGTGACGa > 1:3105302/1‑68 (MQ=255) ggTGAAGGGGAATTAATCGTTCATCCAATCACCCCACAACCAATTGGCGTAGTGACGATTTATCCAggg < 1:3333376/69‑1 (MQ=255) ggTGAAGGGGAATTAATCGTTCATCCAATCACCCCACAACCAATTGGCGTAGTGACGATTTATCCAggg < 1:1889677/69‑1 (MQ=255) ggTGAAGGGGAATTAATCGTTCATCCAATCACCCCACAACCAATTGGCGTAGTGACGATTTATCCAggg < 1:1177715/69‑1 (MQ=255) ggggCATTGATCGTTCATCCAATCACGCCGCAACCaa > 1:2062139/1‑37 (MQ=21) ggggCATTGATCGTTCATCCAATCACGCCGCAACCAATTGGc > 1:1625688/1‑42 (MQ=25) ggggCATTGATCGTTCATCCAATCACGCCGCAACCAATTGGCg > 1:1006061/1‑43 (MQ=25) ggggCATTGATCGTTCATCCAATCACGCCGCAACCAATTGGCg > 1:2352389/1‑43 (MQ=25) ggggCATTGATCGTTCATCCAATCACGCCGCAACCAATTGGCg > 1:422942/1‑43 (MQ=25) ggggCATTGATCGTTCATCCAATCACGCCGCAACCAATTGGCg > 1:1996278/1‑43 (MQ=25) ggggCATTGATCGTTCATCCAATCACGCCGCAACCAATTGGCg > 1:1987074/1‑43 (MQ=25) ggggCATTGATCGTTCATCCAATCACGCCGCAACCAATTGGCg > 1:1704986/1‑43 (MQ=25) ggggCATTGATCGTTCATCCAATCACGCCGCAACCAATTGGCg > 1:52859/1‑43 (MQ=25) ggggCATTGATCGTTCATCCAATCACGCCGCAACCAATTGGCg > 1:1336958/1‑43 (MQ=25) ggggCATTGATCGTTCATCCAATCACGCCGCAACCAATTGGCg > 1:887807/1‑43 (MQ=25) gTTCATCCAATCACCCCACAACCAATTGGCGTAGTGACGATTTATCCAGGGATTTCTGCTGACGTCGTg > 1:2452893/1‑69 (MQ=255) aTCACGCCGCAACCAATTGGCGTAGTGACGATTTATCCGGGGATTTCTGCTGACGTCGTGCGCAAtttt < 1:495672/69‑1 (MQ=255) aTCACGCCGCAACCAATTGGCGTAGTGACGATTTATCCGGGGATTTCTGCTGACGTCGTGCGCAAtttt < 1:2538834/69‑1 (MQ=255) aTCACGCCGCAACCAATTGGCGTAGTGACGATTTATCCGGGGATTTCTGCTGACGTCGTGCGCAAtttt < 1:3362413/69‑1 (MQ=255) aTCACGCCGCAACCAATTGGCGTAGTGACGATTTATCCGGGGATTTCTGCTGACGTCGTGCGCAAtttt < 1:3286217/69‑1 (MQ=255) aTCACGCCGCAACCAATTGGCGTAGTGACGATTTATCCGGGGATTTCTGCTGACGTCGTGCGCAAtttt < 1:2577322/69‑1 (MQ=255) aTCACGCCGCAACCAATTGGCGTAGTGACGATTTATCCGGGGATTTCTGCTGACGTCGTGCGCAAtttt < 1:2517129/69‑1 (MQ=255) aTCACGCCGCAACCAATTGGCGTAGTGACGATTTATCCGGGGATTTCTGCTGACGTCGTGCGCAAtttt < 1:229445/69‑1 (MQ=255) aTCACGCCGCAACCAATTGGCGTAGTGACGATTTATCCGGGGATTTCTGCTGACGTCGTGCGCAAtttt < 1:1890136/69‑1 (MQ=255) aTCACGCCGCAACCAATTGGCGTAGTGACGATTTATCCGGGGATTTCTGCTGACGTCGTGCGCAAtttt < 1:1700683/69‑1 (MQ=255) aTCACGCCGCAACCAATTGGCGTAGTGACGATTTATCCGGGGATTTCTGCTGACGTCGTGCGCAAtttt < 1:1603909/69‑1 (MQ=255) aTCACGCCGCAACCAATTGGCGTAGTGACGATTTATCCGGGGATTTCTGCTGACGTCGTGCGCAAtttt < 1:1570382/69‑1 (MQ=255) aTCACGCCGCAACCAATTGGCGTAGTGACGATTTATCCGGGGATTTCTGCTGACGTCGTGCGCAAtttt < 1:1483324/69‑1 (MQ=255) aTCACGCCGCAACCAATTGGCGTAGTGACGATTTATCCGGGGATTTCTGCTGACGTCGTGCGCAAtttt < 1:1480041/69‑1 (MQ=255) aTCACGCCGCAACCAATTGGCGTAGTGACGATTTATCCGGGGATTTCTGCTGACGTCGTGCGCAAtttt < 1:1429643/69‑1 (MQ=255) aTCACGCCGCAACCAATTGGCGTAGTGACGATTTATCCGGGGATTTCTGCTGACGTCGTGCGCAAtttt < 1:1357580/69‑1 (MQ=255) aTCACGCCGCAACCAATTGGCGTAGTGACGATTTATCCGGGGATTTCTGCTGACGTCGTGCGCAAtttt < 1:1153427/69‑1 (MQ=255) tCACGCCGCAACCAATTGGCGTAGTGACGATTTATCCGGGGATTTCTGCTGACGTCGTGCGCAAtttt < 1:2001810/68‑1 (MQ=255) tCACGCCGCAACCAATTGGCGTAGTGACGATTTATCCGGGGATTTCTGCTGACGTCGTGCGCAAtttt < 1:1641983/68‑1 (MQ=255) | TTCGTCGTTTGAATACGCCACCCGCCCCGCACGGTGAAGGGGAATTAATCGTTCATCCAATCACCCCACAACCAATTGGCGTAGTGACGATTTATCCAGGGATTTCTGCTGACGTCGTGCGCAATTTT > minE/1179110‑1179237 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |