| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,181,303 | A→G | 46.6% | H109R (CAT→CGT) | yeaD → | conserved hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,181,303 | 0 | A | G | 46.6% | 10.0 / 103.8 | 118 | H109R (CAT→CGT) | yeaD | conserved hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base A (17/46); new base G (24/31); total (41/77) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 8.08e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
Only 100 of 127 total aligned reads displayed.
GCACAACAAGGTCTGCCTGCGCACGGTTTTGCCCGCAACCTGCCGTGGACGCTGAAATCACATCATGAAGATGCTGATGGCGTAGCGCTGACTTTTGAATTGACGCAAAGCGAAGAGACGAAAAAATTCTG > minE/1181239‑1181369 | gcACAACAAGGTCTGTCTGCGCACGGTTTTGCCCGCAACCTGCCGTGGACGCTGAAATCACATCATGaa < 1:2087642/69‑1 (MQ=255)gcACAACAAGGTCTGCCTGCGCACGGTTTTGCCCGCAACCTGCCGTGGACGCTGAAATCACATCATGaa < 1:2846767/69‑1 (MQ=255)gcACAACAAGGTCTGCCTGCGCACGGTTTTGCCCGCAACCTGCCGTGGACGCTGAAATCACATCATGaa < 1:2281869/69‑1 (MQ=255)gcACAACAAGGTCTGCCTGCGCACGGTTTTGCCCGCAACCTGCCGTGGACGCTGAAATCACATCATGaa < 1:1637794/69‑1 (MQ=255)gcACAACAAGGTCTGCCTGCGCACGGTTTTGCCCGCAACCTGCCGTGGACGCTGAAATCACATCATGaa < 1:1684771/69‑1 (MQ=255)gcACAACAAGGTCTGCCTGCGCACGGTTTTGCCCGCAACCTGCCGTGGACGCTGAAATCACATCATGaa < 1:279949/69‑1 (MQ=255)gcACAACAAGGTCTGCCTGCGCACGGTTTTGCCCGCAACCTGCCGTGGACGCTGAAATCACATCATGaa < 1:2114716/69‑1 (MQ=255)gcACAACAAGGTCTGCCTGCGCACGGTTTTGCCCGCAACCTGCCGTGGACGCTGAAATCACATCATGaa < 1:740157/69‑1 (MQ=255)gcACAACAAGGTCTGCCTGCGCACGGTTTTGCCCGCAACCTGCCGTGGACGCTGAAATCACATCATGaa < 1:1822952/69‑1 (MQ=255)gcACAACAAGGTCTGCCTGCGCACGGTTTTGCCCGCAACCTGCCGTGGACGCTGAAATCACATCATGaa < 1:2942208/69‑1 (MQ=255)gcACAACAAGGTCTGCCTGCGCACGGTTTTGCCCGCAACCTGCCGTGGACGCTGAAATCACATCATGaa < 1:3051288/69‑1 (MQ=255)gcACAACAAGGTCTGCCTGCGCACGGTTTTGCCCGCAACCTGCCGTGGACGCTGAAATCACATCATGaa < 1:1218878/69‑1 (MQ=255)gcACAACAAGGTCTGCCTGCGCACGGTTTTGCCCGCAACCTGCCGTGGACGCTGAAATCACATCATGaa < 1:182378/69‑1 (MQ=255)gcACAACAAGGTCTGCCTGCGCACGGTTTTGCCCGCAACCTGCCGTGGACGCTGAAATCACATCATGaa < 1:1925952/69‑1 (MQ=255) cacaACAAGGTCTGCCTGCGCACGGTTTTGCCCGCAACCTGCCGTGGACGCTGAAATCACATCATGaa < 1:664689/68‑1 (MQ=255) acaacaAGGTCTGCCTGCGCACGGTTTTGCCCGCAACCTGCCGTGGACGCTGAAATCACATCATGaa < 1:2135703/67‑1 (MQ=255) acaacaAGGTCTGCCTGCGCACGGTTTTGCCCGCAACCTGCCGTGGACGCTGAAATCACATCATGaa < 1:2619425/67‑1 (MQ=255) acaacaAGGTCTGCCTGCGCACGGTTTTGCCCGCAACCTGCCGTGGACGCTGAAATCACATCATGaa < 1:556401/67‑1 (MQ=255) acaacaAGGTCTGCCTGCGCACGGTTTTGCCCGCAACCTGCCGTGGACGCTGAAATCACATCATGaa < 1:884227/67‑1 (MQ=255) acaacaAGGTCTGCCTGCGCACGGTTTTGCCCGCAACCTGCCGTGGACGCTGAAATCACATCATGaa < 1:2424362/67‑1 (MQ=255) acaacaAGGTCTGCCTGCGCACGGTTTTGCCCGCAACCTGCCGTGGACGCTGAAATCACATCATGaa < 1:1133102/67‑1 (MQ=255) acaacaAGGTCTGCCTGCGCAAGGTTTTGCCCGCAACCTGCCGTGGACGCTGAAATCACATCATGaa < 1:3125959/67‑1 (MQ=255) caaGGTCTGCCTGCGCACGGTTTTGCCCGCAACCTGCCGTGGACGCTGAAATCACATCATGAAGATGCt < 1:2236770/69‑1 (MQ=255) caaGGTCTGCCTGCGCACGGTTTTGCCCGCAACCTGCCGTGGACGCTGAAATCACATCATGAAGATGCt < 1:2216666/69‑1 (MQ=255) caaGGTCTGCCTGCGCACGGTTTTGCCCGCAACCTGCCGTGGACGCTGAAATCACATCATGAAGATGCt < 1:450040/69‑1 (MQ=255) aaGGTCTGCCTGCGCACGGTTTTGCCCGCAACCTGCCGTGGACGCTGAAATCACATCATGAAGATGCt < 1:1646883/68‑1 (MQ=255) ctgcctgcGCACGGTTTTGCCCGCAACCTGCCGTGGACGCTGAAATCACATCGTGAAGATGCTGATgg > 1:2466599/1‑68 (MQ=255) ctgcctgcGCACGGTTTTGCCCGCAACCTGCCGTGGACGCTGAAATCACATCGTGAAGATGCTGATgg > 1:2578607/1‑68 (MQ=255) ctgcctgcGCACGGTTTTGCCCGCAACCTGCCGTGGACGCTGAAATCACATCGTGAAGATGCTGATgg > 1:2583236/1‑68 (MQ=255) ctgcctgcGCACGGTTTTGCCCGCAACCTGCCGTGGACGCTGAAATCACATCGTGAAGATGCTGATgg > 1:2660370/1‑68 (MQ=255) ctgcctgcGCACGGTTTTGCCCGCAACCTGCCGTGGACGCTGAAATCACATCGTGAAGATGCTGATgg > 1:2855625/1‑68 (MQ=255) ctgcctgcGCACGGTTTTGCCCGCAACCTGCCGTGGACGCTGAAATCACATCGTGAAGATGCTGATgg > 1:2904210/1‑68 (MQ=255) ctgcctgcGCACGGTTTTGCCCGCAACCTGCCGTGGACGCTGAAATCACATCGTGAAGATGCTGATgg > 1:3068013/1‑68 (MQ=255) ctgcctgcGCACGGTTTTGCCCGCAACCTGCCGTGGACGCTGAAATCACATCGTGAAGATGCTGATgg > 1:3134021/1‑68 (MQ=255) ctgcctgcGCACGGTTTTGCCCGCAACCTGCCGTGGACGCTGAAATCACATCGTGAAGATGCTGATgg > 1:1144057/1‑68 (MQ=255) ctgcctgcGCACGGTTTTGCCCGCAACCTGCCGTGGACGCTGAAATCACATCGTGAAGATGCTGATgg > 1:1454360/1‑68 (MQ=255) ctgcctgcGCACGGTTTTGCCCGCAACCTGCCGTGGACGCTGAAATCACATCGTGAAGATGCTGATgg > 1:739244/1‑68 (MQ=255) ctgcctgcGCACGGTTTTGCCCGCAACCTGCCGTGGACGCTGAAATCACATCGTGAAGATGCTGATgg > 1:1397116/1‑68 (MQ=255) ctgcctgcGCACGGTTTTGCCCGCAACCTGCCGTGGACGCTGAAATCACATCGTGAAGATGCTGATgg > 1:1235439/1‑68 (MQ=255) ctgcctgcGCACGGTTTTGCCCGCAACCTGCCGTGGACGCTGAAATCACATCGTGAAGATGCTGATgg > 1:521125/1‑68 (MQ=255) ctgcctgcGCACGGTTTTGCCCGCAACCTGCCGTGGACGCTGAAATCACATCGTGAAGATGCTGATgg > 1:1373847/1‑68 (MQ=255) ctgcctgcGCACGGTTTTGCCCGCAACCTGCCGTGGACGCTGAAATCACATCGTGAAGATGCTGATgg > 1:1584918/1‑68 (MQ=255) ctgcctgcGCACGGTTTTGCCCGCAACCTGCCGTGGACGCTGAAATCACATCGTGAAGATGCTGATgg > 1:1600194/1‑68 (MQ=255) ctgcctgcGCACGGTTTTGCCCGCAACCTGCCGTGGACGCTGAAATCACATCGTGAAGATGCTGATgg > 1:1634580/1‑68 (MQ=255) ctgcctgcGCACGGTTTTGCCCGCAACCTGCCGTGGACGCTGAAATCACATCATGAAGATGCTGATgg > 1:933176/1‑68 (MQ=255) tgcGCACGGTTTTGCCCGCAACCTGCCGTGGACGCTGAAATCACATCATGAAGATGCTGATGGCGt < 1:1207970/66‑1 (MQ=255) tgcGCACGGTTTTGCCCGCAACCTGCCGTGGACGCTGAAATCACATCATGAAGATGCTGATGGCGt < 1:2506314/66‑1 (MQ=255) tgcGCACGGTTTTGCCAGCAACCTGCCGTGGACGCTGAAATCACATCATGAAGATGCTGATGGCGt < 1:2192079/66‑1 (MQ=255) gcgcACGGTTTTGCCCGCAACCTGCCGTGGACGCTGAAATCACATCATGAAGATGCTGATGGCGt < 1:1462788/65‑1 (MQ=255) cgcACGGTTTTGCCCGCAACCTGCCGTGGACGCTGAAATCACATCATGAAGATGCTGATGGCGTAgcg > 1:1297594/1‑68 (MQ=255) cgcACGGTTTTGCCCGCAACCTGCCGTGGACGCTGAAATCACATCATGAAGATGCTGATGGCGTAgcg > 1:265744/1‑68 (MQ=255) cgcACGGTTTTGCCCGCAACCTGCCGTGGACGCTGAAATCACATCATGAAGATGCTGATGGCGTAgcg > 1:3327626/1‑68 (MQ=255) gCCCGCAACCTGCCGTGGACGCTGAAATCACATCGTGAAGATGCTGATGGTGTAGTGCTAACTTTTGa < 1:1364393/68‑1 (MQ=255) gCCCGCAACCTGCCGTGGACGCTGAAATCACATCGTGAAGATGCTGATGGTGTAGTGCTAACTTTTGa < 1:2892967/68‑1 (MQ=255) gCCCGCAACCTGCCGTGGACGCTGAAATCACATCGTGAAGATGCTGATGGTGTAGTGCTAACTTTTGa < 1:2915817/68‑1 (MQ=255) gCCCGCAACCTGCCGTGGACGCTGAAATCACATCGTGAAGATGCTGATGGTGTAGTGCTAACTTTTGa < 1:784907/68‑1 (MQ=255) gCCCGCAACCTGCCGTGGACGCTGAAATCACATCGTGAAGATGCTGATGGTGTAGTGCTAACTTTTGa < 1:2789442/68‑1 (MQ=255) gCCCGCAACCTGCCGTGGACGCTGAAATCACATCGTGAAGATGCTGATGGTGTAGTGCTAACTTTTGa < 1:3311761/68‑1 (MQ=255) gCCCGCAACCTGCCGTGGACGCTGAAATCACATCGTGAAGATGCTGATGGTGTAGTGCTAACTTTTGa < 1:470919/68‑1 (MQ=255) gCCCGCAACCTGCCGTGGACGCTGAAATCACATCGTGAAGATGCTGATGGTGTAGTGCTAACTTTTGa < 1:672810/68‑1 (MQ=255) gCCCGCAACCTGCCGTGGACGCTGAAATCACATCGTGAAGATGCTGATGGTGTAGTGCTAACTTTTGa < 1:1926510/68‑1 (MQ=255) gCCCGCAACCTGCCGTGGACGCTGAAATCACATCGTGAAGATGCTGATGGTGTAGTGCTAACTTTTGa < 1:43336/68‑1 (MQ=255) gCCCGCAACCTGCCGTGGACGCTGAAATCACATCGTGAAGATGCTGATGGTGTAGTGCTAACTTTTGa < 1:3422060/68‑1 (MQ=255) gCCCGCAACCTGCCGTGGACGCTGAAATCACATCGTGAAGATGCTGATGGTGTAGTGCTAACTTTTGa < 1:2067189/68‑1 (MQ=255) gCCCGCAACCTGCCGTGGACGCTGAAATCACATCGTGAAGATGCTGATGGTGTAGTGCTAACTTTTGa < 1:176861/68‑1 (MQ=255) gCCCGCAACCTGCCGTGGACGCTGAAATCACATCGTGAAGATGCTGATGGTGTAGTGCTAACTTTTGa < 1:1746384/68‑1 (MQ=255) gCCCGCAACCTGCCGTGGACGCTGAAATCACATCGTGAAGATGCTGATGGTGTAGTGCTAACTTTTGa < 1:2210659/68‑1 (MQ=255) gCCCGCAACCTGCCGTGGACGCTGAAATCACATCGTGAAGATGCTGATGGTGTAGTGCTAACTTTTGa < 1:2410989/68‑1 (MQ=255) gCCCGCAACCTGCCGTGGACGCTGAAATCACATCGTGAAGATGCTGATGGTGTAGTGCTAACTTTTGa < 1:611744/68‑1 (MQ=255) gCCCGCAACCTGCCGTGGACGCTGAAATCACATCGTGAAGATGCTGATGGTGTAGTGCTAACTTTTGa < 1:1563768/68‑1 (MQ=255) gCCCGCAACCTGCCGTGGACGCTGAAATCACATCGTGAAGATGCTGATGGTGTAGTGCTAACTTTTGa < 1:2464293/68‑1 (MQ=255) gCCCGCAACCTGCCGTGGACGCTGAAATCACATCGTGAAGATGCTGATGGTGTAGTGCTAACTTTTGa < 1:3329262/68‑1 (MQ=255) gCCCGCAACCTGCCGTGGACGCTGAAATCACATCGTGAAGATGCTGATGGTGTAGTGCTAACTTTTGa < 1:682508/68‑1 (MQ=255) cccGCAACCTGCCGTGGACGCTGAAATCACATCGTGAAGATGCTGATGGTGTAGTGCTAACTTTTGa < 1:3341039/67‑1 (MQ=255) aCCTGCCGTGGACGCTGAAATCACATCGTGAAGATGCTGATGGTGTAGTGCTAACttttta < 1:3050517/61‑3 (MQ=255) gTGGACGCTGAAATCACATCATGAAGATGCTGATGGCGTAGCGCTGACTTTTGAATTGACGCAAAGCg < 1:2422939/68‑1 (MQ=255) gTGGACGCTGAAATCACATCATGAAGATGCTGATGGCGTAGCGCTGACTTTTGAATTGACGCAAAGCg < 1:1324774/68‑1 (MQ=255) gTGGACGCTGAAATCACATCATGAAGATGCTGATGGCGTAGCGCTGACTTTTGAATTGACGCAAAGCg < 1:1439140/68‑1 (MQ=255) gTGGACGCTGAAATCACATCATGAAGATGCTGATGGCGTAGCGCTGACTTTTGAATTGACGCAAAGCg < 1:1736295/68‑1 (MQ=255) gTGGACGCTGAAATCACATCATGAAGATGCTGATGGCGTAGCGCTGACTTTTGAATTGACGCAAAGCg < 1:2049449/68‑1 (MQ=255) gTGGACGCTGAAATCACATCATGAAGATGCTGATGGCGTAGCGCTGACTTTTGAATTGACGCAAAGCg < 1:1830821/68‑1 (MQ=255) gTGGACGCTGAAATCACATCATGAAGATGCTGATGGCGTAGCGCTGACTTTTGAATTGACGCAAAGCg < 1:1889318/68‑1 (MQ=255) gTGGACGCTGAAATCACATCATGAAGATGCTGATGGCGTAGCCCTGACTTTTGAATTGACGCAAAGCg < 1:1932878/68‑1 (MQ=255) gACGCTGAAATCACATCATGAAGATGCTGATGGCGTAgcgc > 1:2690260/1‑41 (MQ=255) gACGCTGAAATCACATCATGAAGATGCTGATGGCGTAGCGCTGACTTTTGAATTGACGCAAAGCGAaga > 1:1229566/1‑69 (MQ=255) gACGCTGAAATCACATCATGAAGATGCTGATGGCGTAGCGCTGACTTTTGAATTGACGCAAAGCGAaga > 1:725564/1‑69 (MQ=255) gACGCTGAAATCACATCATGAAGATGCTGATGGCGTAGCGCTGACTTTTGAATTGACGCAAAGCGAaga > 1:2485905/1‑69 (MQ=255) gACGCTGAAATCACATCATGAAGATGCTGATGGCGTAGCGCTGACTTTTGAATTGACGCAAAGCGAaga > 1:2624875/1‑69 (MQ=255) gACGCTGAAATCACATCATGAAGATGCTGATGGCGTAGCGCTGACTTTTGAATTGACGCAAAGCGAaga > 1:3097767/1‑69 (MQ=255) cGCTGAAATCACATCATGAAGATGCTGATGGCGTAGCGCTGACTTTTGAATTGACGCAAAGCGAagag > 1:1458013/1‑68 (MQ=255) gAAATCACATCATGAAGATGCTGATGGCGTAGCGCTGACTTTTGATTTGACGCAAAGCg < 1:1567840/59‑1 (MQ=255) acaTCATGAAGATGCTGATGGCGTAGCGCTGACTTTTGAATTGACGCAAAGCGAAGAGACGAAAAAAtt > 1:535590/1‑69 (MQ=255) acaTCATGAAGATGCTGATGGCGTAGCGCTGACTTTTGAATTGACGCAAAGCGAAGAGACGAAAAAAtt > 1:2114147/1‑69 (MQ=255) acaTCATGAAGATGCTGATGGCGTAGCGCTGACTTTTGAATTGACGCAAAGCGAAGAGACGAAAAAAtt > 1:1163246/1‑69 (MQ=255) tcatGAAGATGCTGATGGCGTAGCGCTGACTTTTGAATTGACGCAAAGCGAAGAGACGAAAAAATTCt > 1:1409100/1‑68 (MQ=255) tcatGAAGATGCTGATGGCGTAGCGCTGACTTTTGAATTGACGCAAAGCGAAGAGACGAAAAAATTCTg > 1:1144976/1‑69 (MQ=255) tcatGAAGATGCTGATGGCGTAGCGCTGACTTTTGAATTGACGCAAAGCGAAGAGACGAAAAAATTCTg > 1:938726/1‑69 (MQ=255) tcatGAAGATGCTGATGGCGTAGCGCTGACTTTTGAATTGACGCAAAGCGAAGAGACGAAAAAATTCTg > 1:1514607/1‑69 (MQ=255) tcatGAAGATGCTGATGGCGTAGCGCTGACTTTTGAATTGACGCAAAGCGAAGAGACGAAAAAATTCTg > 1:19325/1‑69 (MQ=255) tcatGAAGATGCTGATGGCGTAGCGCTGACTTTTGAATTGACGCAAAGCGAAGAGACGAAAAAATTCTg > 1:322021/1‑69 (MQ=255) | GCACAACAAGGTCTGCCTGCGCACGGTTTTGCCCGCAACCTGCCGTGGACGCTGAAATCACATCATGAAGATGCTGATGGCGTAGCGCTGACTTTTGAATTGACGCAAAGCGAAGAGACGAAAAAATTCTG > minE/1181239‑1181369 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |