| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,182,974 | G→A | 44.0% | G209G (GGC→GGT) | mipA ← | scaffolding protein for murein synthesizing machinery |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,182,974 | 0 | G | A | 44.0% | 14.1 / 43.0 | 59 | G209G (GGC→GGT) | mipA | scaffolding protein for murein synthesizing machinery |
| Reads supporting (aligned to +/- strand): ref base G (25/8); new base A (17/9); total (42/17) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.03e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
ATCGGGCTGTCAGTAACTTCATCAGACAGACGGGTGTAGCGTGCGGTACCGTAAACACTCCAGTCGCCGAGGAAGTTGTAGCTGGCGCTCAGCTCCAGGTAAGGGCTCCAGCTGTCGTTCGGGTTATAGC > minE/1182909‑1183038 | aTCGGGCTGTCAGTAACTTCATCAGACAGACGGGTGTAGCGTGCGGTACCGTAAACACTCCAGTCGCCg > 1:104366/1‑69 (MQ=255)aTCGGGCTGTCAGTAACTTCATCAGACAGACGGGTGTAGCGTGCGGTACCGTAAACACTCCAGTCGCCg > 1:279155/1‑69 (MQ=255) aCTTCATCAGACAGACGGGTGTAGCGTGCGGTACCGTAAACACTCCAGTCGCCGAGGAAGTTGTAGCt > 1:669579/1‑68 (MQ=255) aCTTCATCAGACAGACGGGTGTAGCGTGCGGTACCGTAAACACTCCAGTCGCCGAGGAAGTTGTAGCTg > 1:447427/1‑69 (MQ=255) aCTTCATCAGACAGACGGGTGTAGCGTGCGGTACCGTAAACACTCCAGTCGCCGAGGAAGTTGTAGCTg > 1:3264806/1‑69 (MQ=255) aCTTCATCAGACAGACGGGTGTAGCGTGCGGTACCGTAAACACTCCAGTCGCCGAGGAAGTTGTAGCTg > 1:3115814/1‑69 (MQ=255) aCTTCATCAGACAGACGGGTGTAGCGTGCGGTACCGTAAACACTCCAGTCGCCGAGGAAGTTGTAGCTg > 1:1316745/1‑69 (MQ=255) aCTTCATCAGACAGACGGGTGTAGCGTGCGGTACCGTAAACACTCAAGTCGCCGAGGAAGTTGTAGCTg > 1:2010595/1‑69 (MQ=255) catcaGACAGACGGGTGTAGCGCGCGATACCGTAAACACTCCAGTCACCGAGGAAg > 1:3324622/1‑56 (MQ=255) catcaGACAGACGGGTGTAGCGCGCGATACCGTAAACACTCCAGTCACCGAGGAAGTTGTAGCTGgcg > 1:3095314/1‑68 (MQ=255) catcaGACAGACGGGTGTAGCGCGCGATACCGTAAACACTCCAGTCACCGAGGAAGTTGTAGCTGgcg > 1:860211/1‑68 (MQ=255) catcaGACAGACGGGTGTAGCGCGCGATACCGTAAACACTCCAGTCACCGAGGAAGTTGTAGCTGgcg > 1:1271906/1‑68 (MQ=255) catcaGACAGACGGGTGTAGCGCGCGATACCGTAAACACTCCAGTCACCGAGGAAGTTGTAGCTGgcg > 1:543487/1‑68 (MQ=255) catcaGACAGACGGGTGTAGCGCGCGATACCGTAAACACTCCAGTCACCGAGGAAGTTGTAGCTGgcg > 1:2688537/1‑68 (MQ=255) catcaGACAGACGGGTGTAGCGCGCGATACCGTAAACACTCCAGTCACCGAGGAAGTTGTAGCTGgcg > 1:2247874/1‑68 (MQ=255) catcaGACAGACGGGTGTAGCGCGCGATACCGTAAACACTCCAGTCACCGAGGAAGTTGTAGCTGgcg > 1:322780/1‑68 (MQ=255) catcaGACAGACGGGTGTAGCGCGCGATACCGTAAACACTCCAGTCACCGAGGAAGTTGTAGCTGgcg > 1:1115731/1‑68 (MQ=255) catcaGACAGACGGGTGTAGCGCGCGATACCGTAAACACTCCAGTCACCGAGGAAGTTGTAGCTGgcg > 1:1821699/1‑68 (MQ=255) catcaGACAGACGGGTGTAGCGCGCGATACCGTAAACACTCCAGTCACCGAGGAAGTTGTAGCTGgcg > 1:1821449/1‑68 (MQ=255) catcaGACAGACGGGTGTAGCGCGCGATACCGTAAACACTCCAGTCACCGAGGAAGTTGTAGCTGgcg > 1:181820/1‑68 (MQ=255) catcaGACAGACGGGTGTAGCGCGCGATACCGTAAACACTCCAGTCACCGAGGAAGTTGTAGCTGgcg > 1:1621398/1‑68 (MQ=255) catcaGACAGACGGGTGTAGCGCGCGATACCGTAAACACTCCAGTCACCGAGGAAGTTGTAGCTGgcg > 1:1501490/1‑68 (MQ=255) catcaGACAGACGGGTGTAGCGCGCGATACCGTAAACACTCCAGTCACCGAGGAAGTTGTAGCTGgcg > 1:1476180/1‑68 (MQ=255) catcaGACAGACGGGTGTAGCGCGCGATACCGTAAACACTCCAGTCACAGAGGAAGTTGTAGCTGgcg > 1:1989129/1‑68 (MQ=255) catcaGACAGACGGGTGTAGCGCGCGATACAGTAAACACTCCAGTCACCGAGGAAGTTGTAGCTGgcg > 1:3047824/1‑68 (MQ=255) cagacagaCGGGTGTAGCGTGCGGTACCGTAAACACTCCAGTCGCCGAGGAAGTTGTAGCTGGCGCTCa > 1:129237/1‑69 (MQ=255) cagacagaCGGGTGTAGCGTGCGGTACCGTAAACACTCCAGTCGCCGAGGAAGTTGTAGCTGGCGCTCa > 1:638009/1‑69 (MQ=255) cagacagaCGGGTGTAGCGTGCGGTACCGTAAACACTCCAGTCGCCGAGGAAGTTGTAGCTGGCGCTCa > 1:3360034/1‑69 (MQ=255) cagacagaCGGGTGTAGCGTGCGGTACCGTAAACACTCCAGTCGCCGAGGAAGTTGTAGCTGGCGCTCa > 1:2844313/1‑69 (MQ=255) cagacagaCGGGTGTAGCGTGCGGTACCGTAAACACTCCAGTCGCCGAGGAAGTTGTAGCTGGCGCTCa > 1:33833/1‑69 (MQ=255) gtgtAGTGTGCGGTACCGTAAACACTCCAGTCGCCGAGGAAGTTGTAGCTGGCGCTCAGCTCCAGGTaa < 1:1987286/69‑1 (MQ=255) tgtAGCGCGCGATACCGTAAACACTCCAGTCACCGAGGAAGTTGTAGCTGGCGCTCAGCTCCAGGTAAg < 1:1356811/69‑1 (MQ=255) tgtAGCGCGCGATACCGTAAACACTCCAGTCACCGAGGAAGTTGTAGCTGGCGCTCAGCTCCAGGTAAg < 1:3197695/69‑1 (MQ=255) tgtAGCGCGCGATACCGTAAACACTCCAGTCACCGAGGAAGTTGTAGCTGGCGCTCAGCTCCAGGTAAg < 1:3208510/69‑1 (MQ=255) tgtAGCGCGCGATACCGTAAACACTCCAGTCACCGAGGAAGTTGTAGCTGGCGCTCAGCTCCAGGTAAg < 1:3232300/69‑1 (MQ=255) tgtAGCGCGCGATACCGTAAACACTCCAGTCACCGAGGAAGTTGTAGCTGGCGCTCAGCTCCAGGTAAg < 1:627958/69‑1 (MQ=255) tgtAGCGCGCGATACCGTAAACACTCCAGTCACCGAGGAAGTTGTAGCTGGCGCTCAGCTCCAGGTAAg < 1:1885983/69‑1 (MQ=255) tgtAGCGCGCGATACCGTAAACACTCCAGTCACCGAGGAAGTTGTAGCTGGCGCTCAGCTCCAGGTAAg < 1:1653402/69‑1 (MQ=255) tgtAGCGCGCGATACCGTAAACACTCCAGTCACCGAGGAAGTTGTAGCTGGCGCTCAGCTCCAGGTAAg < 1:739508/69‑1 (MQ=255) tgtAGCGCGCAATACCGTAAACACTCCAGTCACCGAGGAAGTTGTAGCTGGCGCTCAGCTCCAGGTAAg < 1:715994/69‑1 (MQ=255) cGGTACCGTAAACACTCCAGTCGCCGAGGAAGTTGTAGCTGGCGCTCAGCTCCAGGTAAGGGCTCCAGc > 1:2198925/1‑69 (MQ=255) cGGTACCGTAAACACTCCAGTCGCCGAGGAAGTTGTAGCTGGCGCTCAGCTCCAGGTAAGGGCTCCAGc > 1:455610/1‑69 (MQ=255) cGGTACCGTAAACACTCCAGTCGCCGAGGAAGTTGTAGCTGGCGCTCAGCTCCAGGTAAGGGCTCCAGc > 1:150517/1‑69 (MQ=255) gTAAACACTCCAGTCGCCGAGGAAGTTGTATCTGGCGCTCAGCTCCAAGTAAGGGCTCCAGCTGTCGtt > 1:1974442/1‑69 (MQ=255) gTAAACACTCCAGTCGCCGAGGAAGTTGTAGCTGGCGCTCAGCTCCAAGTAAGGGCTCGAGCTGTCGtt > 1:334076/1‑69 (MQ=255) gTAAACACTCCAGTCGCCGAGGAAGTTGTAGCTGGCGCTCAGCTCCAAGTAAGGGCTCCAGCTGTCGtt > 1:460414/1‑69 (MQ=255) gTAAACACTCCAGTCGCCGAGGAAGTTGTAGCTGGCGCTCAGCTCCAAGTAAGGGCTCCAGCTGTCGtt > 1:1060185/1‑69 (MQ=255) gTAAACACTCCAGTCGCCGAGGAAGTTGTAGCTGGCGCTCAGCTCCAAGTAAGGGCTCCAGCTGTCGtt > 1:2815454/1‑69 (MQ=255) gTAAACACTCCAGTCGCCGAGGAAGTTGTAGCTGGCGCTCAGCTCCAAGTAAGGGCTCCAGCTGTCGtt > 1:2766466/1‑69 (MQ=255) gTAAACACTCCAGTCGCCGAGGAAGTTGTAGCTGGCGCTCAGCTCCAAGTAAGGGCTCCAGCTGTCGtt > 1:2441470/1‑69 (MQ=255) gTAAACACTCCAGTCGCCGAGGAAGTTGTAGCTGGCGCTCAGCTCCAAGTAAGGGCTCCAGCTGTCGtt > 1:2006834/1‑69 (MQ=255) gTAAACACTCCAGTCGCCGAGGAAGTTGTAGCTGGCGCTCAGCTCCAAGTAAGGGCTCCAGCTGTCGtt > 1:1374125/1‑69 (MQ=255) cacTCCAGTCGCCGAGGAAGTTGTAGCTGGCGCTCAGCTCCAGGTAAGGGCTCCAGCTGTCGTTCTGGt < 1:2780730/69‑1 (MQ=255) cacTCCAGTCGCCGAGGAAGTTGTAGCTGGCGCTCAGCTCCAGGTAAGGGCTCCAGCTGTCGTTCGGGt < 1:446207/69‑1 (MQ=255) cacTCCAGTCGCCGAGGAAGTTGTAGCTGGCGCTCAGCTCCAGGTAAGGGCTCCAGCTGTCGTTCGGGt < 1:700408/69‑1 (MQ=255) cacTCCAGTCGCCGAGGAAGTTGTAGCTGGCGCTCAGCTCCAGGTAAGGGCTCCAGCTGTCGTTCGGGt < 1:1185253/69‑1 (MQ=255) gTCGCCGAGGAAGTTGTAGCTGGCGCTCAGCTCCAGGTAAGGGCTCCAGCTGTCTTTCGGGTTATAGc < 1:3231579/68‑1 (MQ=255) gTCGCCGAGGAAGTTGTAGCTGGCGCTCAGCTCCAGGTAAGGGCTCCAGCTGTCGTTCGGGTTATAGc < 1:445057/68‑1 (MQ=255) gTCGCCGAGGAAGTTGTAGCTGGCGCTCAGCTCCAGGTAAGGGCTCCAGCTGTCGTTCGGGTTATAGc < 1:2589525/68‑1 (MQ=255) | ATCGGGCTGTCAGTAACTTCATCAGACAGACGGGTGTAGCGTGCGGTACCGTAAACACTCCAGTCGCCGAGGAAGTTGTAGCTGGCGCTCAGCTCCAGGTAAGGGCTCCAGCTGTCGTTCGGGTTATAGC > minE/1182909‑1183038 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |