| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,184,938 | A→C | 41.4% | A301A (GCA→GCC) | yeaG → | conserved hypothetical protein with nucleoside triphosphate hydrolase domain |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,184,938 | 0 | A | C | 41.4% | 34.9 / 103.1 | 104 | A301A (GCA→GCC) | yeaG | conserved hypothetical protein with nucleoside triphosphate hydrolase domain |
| Reads supporting (aligned to +/- strand): ref base A (37/24); new base C (22/21); total (59/45) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.22e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
Only 100 of 104 total aligned reads displayed.
AGGTAACTACAACGGGACGGAAGGTATCTCCGCCCTGCCGTTCAACGGGATTATTCTCGCACACTCGAACGAGTCCGAATGGGTCACTTTCCGTAATAACAAAAACAACGAAGCCTTCCTCG > minE/1184878‑1184999 | aGGTAACTACAACGGGACGGAAGGTATCTCCGCCCTGCCGTTCAACGGGATTATTCTCGCACACTCg > 1:2842206/1‑67 (MQ=255)aGGTAACTACAACGGGACGGAAGGTATCTCCGCCCTGCCGTTCAACGGGATTATTCTCGCACACTCGa > 1:954886/1‑68 (MQ=255)aGGTAACTACAACGGGACGGAAGGTATCTCCGCCCTGCCGTTCAACGGGATTATTCTCGCACACTCGa > 1:1742277/1‑68 (MQ=255)aGGTAACTACAACGGGACGGAAGGTATCTCCGCCCTGCCGTTCAACGGGATTATTCTCGCACACTCGa > 1:678056/1‑68 (MQ=255)aGGTAACTACAACGGGACGGAAGGTATCTCCGCCCTGCCGTTCAACGGGATTATTCTCGCACACTCGa > 1:510698/1‑68 (MQ=255)aGGTAACTACAACGGGACGGAAGGTATCTCCGCCCTGCCGTTCAACGGGATTATTCTCGCACACTCGa > 1:146061/1‑68 (MQ=255) gTAACTACAACGGGACGGAAGGTATCTCCGCCCTGCCGTTCAACGGGATTATTCTCGCACACTCGAACg > 1:2070369/1‑69 (MQ=255) gTAACTACAACGGGACGGAAGGTATCTCCGCCCTGCCGTTCAACGGGATTATTCTCGCACACTCGAACg > 1:628624/1‑69 (MQ=255) gTAACTACAACGGGACGGAAGGTATCTCCGCCCTGCCGTTCAACGGGATTATTCTCGCACACTCGAACg > 1:1288318/1‑69 (MQ=255) gTAACTACAACGGGACGGAAGGTATCTCCGCCCTGCCGTTCAACGGGATTATTCTCGCACACTCGAACg > 1:1565228/1‑69 (MQ=255) gTAACTACAACGGGACGGAAGGTATCTCCGCCCTGCCGTTCAACGGGATTATTCTCGCACACTCGAACg > 1:1920899/1‑69 (MQ=255) gTAACTACAACGGGACGGAAGGTATCTCCGCCCTGCCGTTCAACGGGATTATTCTCGCACACTCGAACg > 1:2645174/1‑69 (MQ=255) tACAACGGGACGGAAGGTATCTCCGCCCTGCCGTTCAACGGGATTATTCTCGCCCACTCg > 1:3317122/1‑60 (MQ=255) tACAACGGGACGGAAGGTATCTCCGCCCTGCCGTTCAACGGGATTATTCTCGCCCACTCGAACGa > 1:1546301/1‑65 (MQ=255) tACAACGGGACGGAAGGTATCTCCGCCCTGCCGTTCAACGGGATTATTCTCGCCCACTCGAACGAATc > 1:1689681/1‑68 (MQ=255) tACAACGGGACGGAAGGTATCTCCGCCCTGCCGTTCAACGGGATTATTCTCGCCCACTCGAACGAATc > 1:1977447/1‑68 (MQ=255) tACAACGGGACGGAAGGTATCTCCGCCCTGCCGTTCAACGGGATTATTCTCGCCCACTCGAACGAATc > 1:2900732/1‑68 (MQ=255) tACAACGGGACGGAAGGTATCTCCGCCCTGCCGTTCAACGGGATTATTCTCGCCCACTCGAACGAATc > 1:28702/1‑68 (MQ=255) tACAACGGGACGGAAGGTATCTCCGCCCTGCCGTTCAACGGGATTATTCTCGCCCACTCGAACGAATc > 1:2844698/1‑68 (MQ=255) tACAACGGGACGGAAGGTATCTCCGCCCTGCCGTTCAACGGGATTATTCTCGCCCACTCGAACGAATc > 1:2717705/1‑68 (MQ=255) tACAACGGGACGGAAGGTATCTCCGCCCTGCCGTTCAACGGGATTATTCTCGCCCACTCGAACGAATc > 1:2743781/1‑68 (MQ=255) tACAACGGGACGGAAGGTATCTCCGCCCTGCCGTTCAACGGGATTATTCTCGCCCACTCGAACGAATc > 1:2179299/1‑68 (MQ=255) tACAACGGGACGGAAGGTATCTCCGCCCTGCCGTTCAACGGGATTATTCTCGCCCACTCGAACGAATc > 1:3320269/1‑68 (MQ=255) tACAACGGGACGGAAGGTATCTCCGCCCTGCCGTTCAACGGGATTATTCTCGCCCACTCGAACGAATc > 1:1542345/1‑68 (MQ=255) tACAACGGGACGGAAGGTATCTCCGCCCTGCCGTTCAACGGGATTATTCTCGCCCACTCGAACGAATc > 1:3329638/1‑68 (MQ=255) tACAACGGGACGGAAGGTATCTCCGCCCTGCCGTTCAACGGGATTATTCTCGCCCACTCGAACGAATc > 1:3358513/1‑68 (MQ=255) tACAACGGGACGGAAGGTATCTCCGCCCTGCCGTTCAACGGGATTATTCTCGCCCACTCGAACGAATc > 1:251209/1‑68 (MQ=255) tACAACGGGACGGAAGGTATCTCCGCCCTGCCGTTCAACGGGATTATTCTCGCCCACTCGAACGAATc > 1:705320/1‑68 (MQ=255) tACAACGGGACGGAAGGTATCTCCGCCCTGCCGTTCAACGGGATTATTCTCGCCCACTCGAACGAATc > 1:707172/1‑68 (MQ=255) tACAACGGGACGGAAGGTATCTCCGCCCTGCCGTTCAACGGGATTATTCTCGCCCACTCGAACGAATc > 1:8789/1‑68 (MQ=255) tACAACGGGACGGAAGGTATCTCCGCCCTGCCGTTCAACGGGATTATTCTCGCCCACTCGAACGAATc > 1:2440961/1‑68 (MQ=255) tACAACGGGACGGAAGGTATCTCCGCCCTGCCGTTCAACGGGATTATTCTCGCCCACTCGAACGAATc > 1:10002/1‑68 (MQ=255) tACAACGGGACGGAAGGTATCTCCGCCCTGCCGTTCAACGGGATTATTCTCGCACACTCGAACGAGTc > 1:2547922/1‑68 (MQ=255) tACAACGGGACGGAAGGTATCTCCGCCCTGCCGTTCAACGGGATTATTCTCGCACACTCGAACGAGTc > 1:2338780/1‑68 (MQ=255) tACAACGGGACGGAAGGTATCTCCGCCCTGCCGTTCAACGGGATTATTCTCGCACACTCGAACGAGTc > 1:1045779/1‑68 (MQ=255) aCGGGACGGAAGGTATCTCCGCCCTGCCGTTCAACGGGATTATTCTCGCACACTCGAACGAGTCCGaa < 1:2789876/68‑1 (MQ=255) aCGGGACGGAAGGTATCTCCGCCCTGCCGTTCAACGGGATTATTCTCGCACACTCGAACGAGTCCGaa < 1:2675613/68‑1 (MQ=255) aCGGGACGGAAGGTATCTCCGCCCTGCCGTTCAACGGGATTATTCTCGCACACTCGAACGAGTCCGaa < 1:2868074/68‑1 (MQ=255) aCGGGACGGAAGGTATCTCCGCCCTGCCGTTCAACGGGATTATTCTCGCACACTCGAACGAGTCCGaa < 1:304005/68‑1 (MQ=255) aCGGGACGGAAGGTATCTCCGCCCTGCCGTTCAACGGGATTATTCTCGCACACTCGAACGAGTCCGaa < 1:263091/68‑1 (MQ=255) aCGGGACGGAAGGTATCTCCGCCCTGCCGTTCAACGGGATTATTCTCGCACACTCGAACGAGTCCGaa < 1:3065703/68‑1 (MQ=255) aCGGGACGGAAGGTATCTCCGCCCTGCCGTTCAACGGGATTATTCTCGCACACTCGAACGAGTCCGaa < 1:3267920/68‑1 (MQ=255) aCGGGACGGAAGGTATCTCCGCCCTGCCGTTCAACGGGATTATTCTCGCACACTCGAACGAGTCCGaa < 1:3384422/68‑1 (MQ=255) aCGGGACGGAAGGTATCTCCGCCCTGCCGTTCAACGGGATTATTCTCGCACACTCGAACGAGTCCGaa < 1:2345941/68‑1 (MQ=255) aCGGGACGGAAGGTATCTCCGCCCTGCCGTTCAACGGGATTATTCTCGCACACTCGAACGAGTCCGaa < 1:101381/68‑1 (MQ=255) aCGGGACGGAAGGTATCTCCGCCCTGCCGTTCAACGGGATTATTCTCGCACACTCGAACGAGTCCGaa < 1:1898560/68‑1 (MQ=255) aCGGGACGGAAGGTATCTCCGCCCTGCCGTTCAACGGGATTATTCTCGCACACTCGAACGAGTCCGaa < 1:2252624/68‑1 (MQ=255) aCGGGACGGAAGGTATCTCCGCCCTGCCGTTCAACGGGATTATTCTCGCACACTCGAACGAGTCCGaa < 1:1120019/68‑1 (MQ=255) aCGGGACGGAAGGTATCTCCGCCCTGCCGTTCAACGGGATTATTCTCGCACACTCGAACGAGTCCGaa < 1:1795405/68‑1 (MQ=255) aCGGGACGGAAGGTATCTCCGCCCTGCCGTTCAACGGGATTATTCTCGCACACTCGAACGAGTCCGaa < 1:1232/68‑1 (MQ=255) aCGGGACGGAAGGTATCTCCGCCCTGCCGTTCAACGGGATTATTCTCGCACACTCGAACGAGTCCGaa < 1:2147796/68‑1 (MQ=255) aCGGGACGGAAGGTATCTCCGCCCTGCCGTTCAACGGGATTATTCTCGCACACTCGAACGAGTCCGaa < 1:1510141/68‑1 (MQ=255) aGGTATCTCCGCCCTGCCGTTCAACGGGATTATTCTCGCCCACTCGAACGAATCCGAATGGGTCACttt < 1:1324747/69‑1 (MQ=255) aGGTATCTCCGCCCTGCCGTTCAACGGGATTATTCTCGCCCACTCGAACGAATCCGAATGGGTCACttt < 1:2906404/69‑1 (MQ=255) aGGTATCTCCGCCCTGCCGTTCAACGGGATTATTCTCGCCCACTCGAACGAATCCGAATGGGTCACttt < 1:1648191/69‑1 (MQ=255) aGGTATCTCCGCCCTGCCGTTCAACGGGATTATTCTCGCCCACTCGAACGAATCCGAATGGGTCACttt < 1:1565782/69‑1 (MQ=255) aGGTATCTCCGCCCTGCCGTTCAACGGGATTATTCTCGCCCACTCGAACGAATCCGAATGGGTCACttt < 1:3353731/69‑1 (MQ=255) aGGTATCTCCGCCCTGCCGTTCAACGGGATTATTCTCGCCCACTCGAACGAATCCGAATGGGTCACttt < 1:3357720/69‑1 (MQ=255) aGGTATCTCCGCCCTGCCGTTCAACGGGATTATTCTCGCCCACTCGAACGAATCCGAATGGGTCACttt < 1:1412742/69‑1 (MQ=255) aGGTATCTCCGCCCTGCCGTTCAACGGGATTATTCTCGCCCACTCGAACGAATCCGAATGGGTCACttt < 1:491937/69‑1 (MQ=255) aGGTATCTCCGCCCTGCCGTTCAACGGGATTATTCTCGCCCACTCGAACGAATCCGAATGGGTCACttt < 1:1651484/69‑1 (MQ=255) aGGTATCTCCGCCCTGCCGTTCAACGGGATTATTCTCGCCCACTCGAACGAATCCGAATGGGTCACttt < 1:1286716/69‑1 (MQ=255) aGGTATCTCCGCCCTGCCGTTCAACGGGATTATTCTCGCCCACTCGAACGAATCCGAATGGGTCACttt < 1:1197374/69‑1 (MQ=255) aGGTATCTCCGCCCTGCCGTTCAACGGGATTATTCTCGCCCACTCGAACGAATCCGAATGGGTCACttt < 1:77319/69‑1 (MQ=255) aGGTATCTCCGCCCTGCCGTTCAACGGGATTATTCTCGCCCACTCGAACGAATCCGAATGGGTCACttt < 1:78149/69‑1 (MQ=255) aGGTATCTCCGCCCTGCCGTTCAACGGGATTATTCTCGCCCACTCGAACGAATCCGAATGGGTCACttt < 1:847954/69‑1 (MQ=255) aGGTATCTCCGCCCTGCCGTTCAACGGGATTATTCTCGCCCACTCGAACGAATCCGAATGGGTCACttt < 1:1043014/69‑1 (MQ=255) aGGTATCTCCGCCCTGCCGTTCAACGGGATTATTCTCGCCCACTCGAACGAATCCGAATGGGTCACttt < 1:1936202/69‑1 (MQ=255) aGGTATCTCCGCCCTGCCGTTCAACGGGATTATTCTCGCCCACTCGAACGAATCCGAATGGGTCACttt < 1:2478477/69‑1 (MQ=255) aGGTATCTCCGCCCTGCCGTTCAACGGGATTATTCTCGCCCACTCGAACGAATCCGAATGGGTCACttt < 1:2607589/69‑1 (MQ=255) aGGTATCTCCGCCCTGCCGTTCAACGGGATTATTCTCGCCCACTCGAACGAATCCGAATGGGTCACttt < 1:1955880/69‑1 (MQ=255) aGGTATCTCCGCCCTGCCGTTCAACGGGATTATTCTCGCCCACTCGAACGAATCCGAATGGGTCACttt < 1:2809033/69‑1 (MQ=255) tATCTCCGCCCTGCCGTTCAACGGGATTATTCTCGCACACTCGa > 1:1962718/1‑44 (MQ=255) tATCTCCGCCCTGCCGTTCAACGGGATTATTCTCGCACACTCGAACGAGTCCGAATGGGTCACTTTcc > 1:2370141/1‑68 (MQ=255) tATCTCCGCCCTGCCGTTCAACGGGATTATTCTCGCACACTCGAACGAGTCCGAATGGGTCACTTTcc > 1:2216135/1‑68 (MQ=255) tATCTCCGCCCTGCCGTTCAACGGGATTATTCTCGCACACTCGAACGAGTCCGAATGGGTCACTTTcc > 1:764484/1‑68 (MQ=255) tATCTCCGCCCTGCCGTTCAACGGGATTATTCTCGCACACTCGAACGAGTCCGAATGGGTCACTTTcc > 1:2039434/1‑68 (MQ=255) tATCTCCGCCCTGCCGTTCAACGGGATTATTCTCGCACACTCGAACGAGTCCGAATGGGTCACTTTcc > 1:1948155/1‑68 (MQ=255) tATCTCCGCCCTGCCGTTCAACGGGATTATTCTCGCACACTCGAACGAGTCCGAATGGGTCACTTTc > 1:1928173/1‑67 (MQ=255) tATCTCAGCCCTGCCGTTCAACGGGATTATTCTCGCACACTCGAACGAGTCCGAATGGGTCACTTTcc > 1:435550/1‑68 (MQ=255) tcCGCCCTGCCGTTCAACGGGATTATTCTCGCACACTCGAACGAGTCCGAATGGGTCACTTTCCGtaat > 1:1738319/1‑69 (MQ=255) tcCGCCCTGCCGTTCAACGGGATTATTCTCGCACACTCGAACGAGTCCGAATGGGTCACTTTCCGtaat > 1:215545/1‑69 (MQ=255) tcCGCCCTGCCGTTCAACGGGATTATTCTCGCACACTCGAACGAGTCCGAATGGGTCACTTTCCGtaat > 1:196967/1‑69 (MQ=255) cTGCCGTTCAACGGGATTATTCTCGCACACTCGAACGAGTCCGaa < 1:2635563/45‑1 (MQ=255) aCGGGATTATTCTCGCACACTCGAACGAGTCCGaa < 1:2701793/35‑1 (MQ=255) tattCTCGCACACTCGAACGAGTCCGAATGGGTCACTTTCCGTAATAACAAAAACAACGAAGCCTTcc > 1:1987046/1‑68 (MQ=255) tattCTCGCACACTCGAACGAGTCCGAATGGGTCACTTTCCGTAATAACAAAAACAACGAAGCCTTcc > 1:3376656/1‑68 (MQ=255) tattCTCGCACACTCGAACGAGTCCGAATGGGTCACTTTCCGTAATAACAAAAACAACGAAGCCTTcc > 1:638514/1‑68 (MQ=255) tattCTCGCACACTCGAACGAGTCCGAATGGGTCACTTTCCGTAATAACAAAAACAACGAAGCCTTcc > 1:1211096/1‑68 (MQ=255) tattCTCGCACACTCGAACGAGTCCGAATGGGTCACTTTCCGTAATAACAAAAACAACGAAGCCTTcc > 1:2305504/1‑68 (MQ=255) tattCTCGCACACTCGAACGAGTCCGAATGGGTCACTTTCCGTAATAACAAAAACAACGAAGCCTTcc > 1:3071976/1‑68 (MQ=255) tattCTCGCACACTCGAACGAGTCCGAATGGGTCACTTTCCGTAATAACAAAAACAACGAAGCCTTcc > 1:3371534/1‑68 (MQ=255) tattCTCGCACACTCGAACGAGTCCGAATGGGTCACTTTCCGTAATAACAAAAACAACGAAGCCTTc > 1:253470/1‑67 (MQ=255) tattCTCGCACACTCGAACGAGTCCGAATCGGTCACTTTCCGTAATAACAAAAACAACGAAGCCTTcc > 1:460433/1‑68 (MQ=255) tctcGCCCACTCGAACGAATCCGAATGGGTCACttt < 1:38401/36‑1 (MQ=255) tctcGCACACTCGAACGAGTCCGAATGGGTCACTTTCCGTAATAACAAAAACAACGAAGCCTTCCTCg < 1:607930/68‑1 (MQ=255) tctcGCACACTCGAACGAGTCCGAATGGGTCACTTTCCGTAATAACAAAAACAACGAAGCCTTCCTCg < 1:2616770/68‑1 (MQ=255) tctcGCACACTCGAACGAGTCCGAATGGGTCACTTTCCGTAATAACAAAAACAACGAAGCCTTCCTCg < 1:208610/68‑1 (MQ=255) tctcGCACACTCGAACGAGTCCGAATGGGTCACTTTCCGTAATAACAAAAACAACGAAGCCTTCCTCg < 1:151118/68‑1 (MQ=255) tctcGCACACTCGAACGAGTCCGAATGGGTCACTTTCCGTAATAACAAAAACAACGAAGCCTTCCTCg < 1:3358334/68‑1 (MQ=255) | AGGTAACTACAACGGGACGGAAGGTATCTCCGCCCTGCCGTTCAACGGGATTATTCTCGCACACTCGAACGAGTCCGAATGGGTCACTTTCCGTAATAACAAAAACAACGAAGCCTTCCTCG > minE/1184878‑1184999 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |