| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,184,950 | G→A | 40.5% | E305E (GAG→GAA) | yeaG → | conserved hypothetical protein with nucleoside triphosphate hydrolase domain |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,184,950 | 0 | G | A | 40.5% | 46.0 / 70.5 | 102 | E305E (GAG→GAA) | yeaG | conserved hypothetical protein with nucleoside triphosphate hydrolase domain |
| Reads supporting (aligned to +/- strand): ref base G (33/27); new base A (20/21); total (53/49) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.51e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.82e-01 | |||||||||||
Only 100 of 102 total aligned reads displayed.
TACAACGGGACGGAAGGTATCTCCGCCCTGCCGTTCAACGGGATTATTCTCGCACACTCGAACGAGTCCGAATGGGTCACTTTCCGTAATAACAAAAACAACGAAGCCTTCCTCGATCGTGTTTACA > minE/1184885‑1185011 | tACAACGGGACGGAAGGTATCTCCGCCCTGCCGTTCAACGGGATTATTCTCGCCCACTCGAACGAATc > 1:1810067/1‑68 (MQ=255)tACAACGGGACGGAAGGTATCTCCGCCCTGCCGTTCAACGGGATTATTCTCGCCCACTCGAACGAATc > 1:2440961/1‑68 (MQ=255)tACAACGGGACGGAAGGTATCTCCGCCCTGCCGTTCAACGGGATTATTCTCGCCCACTCGAACGAATc > 1:251209/1‑68 (MQ=255)tACAACGGGACGGAAGGTATCTCCGCCCTGCCGTTCAACGGGATTATTCTCGCCCACTCGAACGAATc > 1:10002/1‑68 (MQ=255)tACAACGGGACGGAAGGTATCTCCGCCCTGCCGTTCAACGGGATTATTCTCGCCCACTCGAACGAATc > 1:2717705/1‑68 (MQ=255)tACAACGGGACGGAAGGTATCTCCGCCCTGCCGTTCAACGGGATTATTCTCGCCCACTCGAACGAATc > 1:2179299/1‑68 (MQ=255)tACAACGGGACGGAAGGTATCTCCGCCCTGCCGTTCAACGGGATTATTCTCGCCCACTCGAACGAATc > 1:2743781/1‑68 (MQ=255)tACAACGGGACGGAAGGTATCTCCGCCCTGCCGTTCAACGGGATTATTCTCGCCCACTCGAACGAATc > 1:2844698/1‑68 (MQ=255)tACAACGGGACGGAAGGTATCTCCGCCCTGCCGTTCAACGGGATTATTCTCGCCCACTCGAACGAATc > 1:1977447/1‑68 (MQ=255)tACAACGGGACGGAAGGTATCTCCGCCCTGCCGTTCAACGGGATTATTCTCGCCCACTCGAACGAATc > 1:28702/1‑68 (MQ=255)tACAACGGGACGGAAGGTATCTCCGCCCTGCCGTTCAACGGGATTATTCTCGCCCACTCGAACGAATc > 1:2900732/1‑68 (MQ=255)tACAACGGGACGGAAGGTATCTCCGCCCTGCCGTTCAACGGGATTATTCTCGCCCACTCGAACGAATc > 1:3320269/1‑68 (MQ=255)tACAACGGGACGGAAGGTATCTCCGCCCTGCCGTTCAACGGGATTATTCTCGCCCACTCGAACGAATc > 1:1689681/1‑68 (MQ=255)tACAACGGGACGGAAGGTATCTCCGCCCTGCCGTTCAACGGGATTATTCTCGCCCACTCGAACGAATc > 1:3329638/1‑68 (MQ=255)tACAACGGGACGGAAGGTATCTCCGCCCTGCCGTTCAACGGGATTATTCTCGCCCACTCGAACGAATc > 1:3358513/1‑68 (MQ=255)tACAACGGGACGGAAGGTATCTCCGCCCTGCCGTTCAACGGGATTATTCTCGCCCACTCGAACGAATc > 1:8789/1‑68 (MQ=255)tACAACGGGACGGAAGGTATCTCCGCCCTGCCGTTCAACGGGATTATTCTCGCCCACTCGAACGAATc > 1:1542345/1‑68 (MQ=255)tACAACGGGACGGAAGGTATCTCCGCCCTGCCGTTCAACGGGATTATTCTCGCCCACTCGAACGAATc > 1:705320/1‑68 (MQ=255)tACAACGGGACGGAAGGTATCTCCGCCCTGCCGTTCAACGGGATTATTCTCGCCCACTCGAACGAATc > 1:707172/1‑68 (MQ=255)tACAACGGGACGGAAGGTATCTCCGCCCTGCCGTTCAACGGGATTATTCTCGCACACTCGAACGAGTc > 1:2338780/1‑68 (MQ=255)tACAACGGGACGGAAGGTATCTCCGCCCTGCCGTTCAACGGGATTATTCTCGCACACTCGAACGAGTc > 1:1045779/1‑68 (MQ=255) aCGGGACGGAAGGTATCTCCGCCCTGCCGTTCAACGGGATTATTCTCGCACACTCGAACGAGTCCGaa < 1:1232/68‑1 (MQ=255) aCGGGACGGAAGGTATCTCCGCCCTGCCGTTCAACGGGATTATTCTCGCACACTCGAACGAGTCCGaa < 1:101381/68‑1 (MQ=255) aCGGGACGGAAGGTATCTCCGCCCTGCCGTTCAACGGGATTATTCTCGCACACTCGAACGAGTCCGaa < 1:2345941/68‑1 (MQ=255) aCGGGACGGAAGGTATCTCCGCCCTGCCGTTCAACGGGATTATTCTCGCACACTCGAACGAGTCCGaa < 1:263091/68‑1 (MQ=255) aCGGGACGGAAGGTATCTCCGCCCTGCCGTTCAACGGGATTATTCTCGCACACTCGAACGAGTCCGaa < 1:2675613/68‑1 (MQ=255) aCGGGACGGAAGGTATCTCCGCCCTGCCGTTCAACGGGATTATTCTCGCACACTCGAACGAGTCCGaa < 1:2252624/68‑1 (MQ=255) aCGGGACGGAAGGTATCTCCGCCCTGCCGTTCAACGGGATTATTCTCGCACACTCGAACGAGTCCGaa < 1:1120019/68‑1 (MQ=255) aCGGGACGGAAGGTATCTCCGCCCTGCCGTTCAACGGGATTATTCTCGCACACTCGAACGAGTCCGaa < 1:2147796/68‑1 (MQ=255) aCGGGACGGAAGGTATCTCCGCCCTGCCGTTCAACGGGATTATTCTCGCACACTCGAACGAGTCCGaa < 1:2789876/68‑1 (MQ=255) aCGGGACGGAAGGTATCTCCGCCCTGCCGTTCAACGGGATTATTCTCGCACACTCGAACGAGTCCGaa < 1:3384422/68‑1 (MQ=255) aCGGGACGGAAGGTATCTCCGCCCTGCCGTTCAACGGGATTATTCTCGCACACTCGAACGAGTCCGaa < 1:304005/68‑1 (MQ=255) aCGGGACGGAAGGTATCTCCGCCCTGCCGTTCAACGGGATTATTCTCGCACACTCGAACGAGTCCGaa < 1:3065703/68‑1 (MQ=255) aCGGGACGGAAGGTATCTCCGCCCTGCCGTTCAACGGGATTATTCTCGCACACTCGAACGAGTCCGaa < 1:1898560/68‑1 (MQ=255) aCGGGACGGAAGGTATCTCCGCCCTGCCGTTCAACGGGATTATTCTCGCACACTCGAACGAGTCCGaa < 1:2868074/68‑1 (MQ=255) aCGGGACGGAAGGTATCTCCGCCCTGCCGTTCAACGGGATTATTCTCGCACACTCGAACGAGTCCGaa < 1:1795405/68‑1 (MQ=255) aCGGGACGGAAGGTATCTCCGCCCTGCCGTTCAACGGGATTATTCTCGCACACTCGAACGAGTCCGaa < 1:3267920/68‑1 (MQ=255) aCGGGACGGAAGGTATCTCCGCCCTGCCGTTCAACGGGATTATTCTCGCACACTCGAACGAGTCCGaa < 1:1510141/68‑1 (MQ=255) aGGTATCTCCGCCCTGCCGTTCAACGGGATTATTCTCGCCCACTCGAACGAATCCGAATGGGTCACttt < 1:2607589/69‑1 (MQ=255) aGGTATCTCCGCCCTGCCGTTCAACGGGATTATTCTCGCCCACTCGAACGAATCCGAATGGGTCACttt < 1:3357720/69‑1 (MQ=255) aGGTATCTCCGCCCTGCCGTTCAACGGGATTATTCTCGCCCACTCGAACGAATCCGAATGGGTCACttt < 1:3353731/69‑1 (MQ=255) aGGTATCTCCGCCCTGCCGTTCAACGGGATTATTCTCGCCCACTCGAACGAATCCGAATGGGTCACttt < 1:847954/69‑1 (MQ=255) aGGTATCTCCGCCCTGCCGTTCAACGGGATTATTCTCGCCCACTCGAACGAATCCGAATGGGTCACttt < 1:491937/69‑1 (MQ=255) aGGTATCTCCGCCCTGCCGTTCAACGGGATTATTCTCGCCCACTCGAACGAATCCGAATGGGTCACttt < 1:78149/69‑1 (MQ=255) aGGTATCTCCGCCCTGCCGTTCAACGGGATTATTCTCGCCCACTCGAACGAATCCGAATGGGTCACttt < 1:2809033/69‑1 (MQ=255) aGGTATCTCCGCCCTGCCGTTCAACGGGATTATTCTCGCCCACTCGAACGAATCCGAATGGGTCACttt < 1:77319/69‑1 (MQ=255) aGGTATCTCCGCCCTGCCGTTCAACGGGATTATTCTCGCCCACTCGAACGAATCCGAATGGGTCACttt < 1:2906404/69‑1 (MQ=255) aGGTATCTCCGCCCTGCCGTTCAACGGGATTATTCTCGCCCACTCGAACGAATCCGAATGGGTCACttt < 1:1565782/69‑1 (MQ=255) aGGTATCTCCGCCCTGCCGTTCAACGGGATTATTCTCGCCCACTCGAACGAATCCGAATGGGTCACttt < 1:1648191/69‑1 (MQ=255) aGGTATCTCCGCCCTGCCGTTCAACGGGATTATTCTCGCCCACTCGAACGAATCCGAATGGGTCACttt < 1:2478477/69‑1 (MQ=255) aGGTATCTCCGCCCTGCCGTTCAACGGGATTATTCTCGCCCACTCGAACGAATCCGAATGGGTCACttt < 1:1651484/69‑1 (MQ=255) aGGTATCTCCGCCCTGCCGTTCAACGGGATTATTCTCGCCCACTCGAACGAATCCGAATGGGTCACttt < 1:1043014/69‑1 (MQ=255) aGGTATCTCCGCCCTGCCGTTCAACGGGATTATTCTCGCCCACTCGAACGAATCCGAATGGGTCACttt < 1:1197374/69‑1 (MQ=255) aGGTATCTCCGCCCTGCCGTTCAACGGGATTATTCTCGCCCACTCGAACGAATCCGAATGGGTCACttt < 1:1412742/69‑1 (MQ=255) aGGTATCTCCGCCCTGCCGTTCAACGGGATTATTCTCGCCCACTCGAACGAATCCGAATGGGTCACttt < 1:1324747/69‑1 (MQ=255) aGGTATCTCCGCCCTGCCGTTCAACGGGATTATTCTCGCCCACTCGAACGAATCCGAATGGGTCACttt < 1:1286716/69‑1 (MQ=255) aGGTATCTCCGCCCTGCCGTTCAACGGGATTATTCTCGCCCACTCGAACGAATCCGAATGGGTCACttt < 1:1936202/69‑1 (MQ=255) aGGTATCTCCGCCCTGCCGTTCAACGGGATTATTCTCGCCCACTCGAACGAATCCGAATGGGTCACttt < 1:1955880/69‑1 (MQ=255) tATCTCCGCCCTGCCGTTCAACGGGATTATTCTCGCACACTCGAACGAGTCCGAATGGGTCACTTTcc > 1:2370141/1‑68 (MQ=255) tATCTCCGCCCTGCCGTTCAACGGGATTATTCTCGCACACTCGAACGAGTCCGAATGGGTCACTTTcc > 1:3329072/1‑68 (MQ=255) tATCTCCGCCCTGCCGTTCAACGGGATTATTCTCGCACACTCGAACGAGTCCGAATGGGTCACTTTcc > 1:764484/1‑68 (MQ=255) tATCTCCGCCCTGCCGTTCAACGGGATTATTCTCGCACACTCGAACGAGTCCGAATGGGTCACTTTcc > 1:1948155/1‑68 (MQ=255) tATCTCCGCCCTGCCGTTCAACGGGATTATTCTCGCACACTCGAACGAGTCCGAATGGGTCACTTTcc > 1:2039434/1‑68 (MQ=255) tATCTCCGCCCTGCCGTTCAACGGGATTATTCTCGCACACTCGAACGAGTCCGAATGGGTCACTTTcc > 1:2216135/1‑68 (MQ=255) tATCTCCGCCCTGCCGTTCAACGGGATTATTCTCGCACACTCGAACGAGTCCGAATGGGTCACTTTc > 1:1928173/1‑67 (MQ=255) tATCTCAGCCCTGCCGTTCAACGGGATTATTCTCGCACACTCGAACGAGTCCGAATGGGTCACTTTcc > 1:435550/1‑68 (MQ=255) tcCGCCCTGCCGTTCAACGGGATTATTCTCGCACACTCGAACGAGTCCGAATGGGTCACTTTCCGtaat > 1:196967/1‑69 (MQ=255) tcCGCCCTGCCGTTCAACGGGATTATTCTCGCACACTCGAACGAGTCCGAATGGGTCACTTTCCGtaat > 1:215545/1‑69 (MQ=255) tcCGCCCTGCCGTTCAACGGGATTATTCTCGCACACTCGAACGAGTCCGAATGGGTCACTTTCCGtaat > 1:1738319/1‑69 (MQ=255) cTGCCGTTCAACGGGATTATTCTCGCACACTCGAACGAGTCCGaa < 1:2635563/45‑1 (MQ=255) aCGGGATTATTCTCGCACACTCGAACGAGTCCGaa < 1:2701793/35‑1 (MQ=255) tattCTCGCACACTCGAACGAGTCCGAATGGGTCACTTTCCGTAATAACAAAAACAACGAAGCCTTcc > 1:1987046/1‑68 (MQ=255) tattCTCGCACACTCGAACGAGTCCGAATGGGTCACTTTCCGTAATAACAAAAACAACGAAGCCTTcc > 1:3376656/1‑68 (MQ=255) tattCTCGCACACTCGAACGAGTCCGAATGGGTCACTTTCCGTAATAACAAAAACAACGAAGCCTTcc > 1:3371534/1‑68 (MQ=255) tattCTCGCACACTCGAACGAGTCCGAATGGGTCACTTTCCGTAATAACAAAAACAACGAAGCCTTcc > 1:2305504/1‑68 (MQ=255) tattCTCGCACACTCGAACGAGTCCGAATGGGTCACTTTCCGTAATAACAAAAACAACGAAGCCTTcc > 1:638514/1‑68 (MQ=255) tattCTCGCACACTCGAACGAGTCCGAATGGGTCACTTTCCGTAATAACAAAAACAACGAAGCCTTcc > 1:3071976/1‑68 (MQ=255) tattCTCGCACACTCGAACGAGTCCGAATGGGTCACTTTCCGTAATAACAAAAACAACGAAGCCTTcc > 1:1211096/1‑68 (MQ=255) tattCTCGCACACTCGAACGAGTCCGAATGGGTCACTTTCCGTAATAACAAAAACAACGAAGCCTTc > 1:253470/1‑67 (MQ=255) tattCTCGCACACTCGAACGAGTCCGAATCGGTCACTTTCCGTAATAACAAAAACAACGAAGCCTTcc > 1:460433/1‑68 (MQ=255) tctcGCCCACTCGAACGAATCCGAATGGGTCACttt < 1:38401/36‑1 (MQ=255) tctcGCACACTCGAACGAGTCCGAATGGGTCACTTTCCGTAATAACAAAAACAACGAAGCCTTCCTCg < 1:607930/68‑1 (MQ=255) tctcGCACACTCGAACGAGTCCGAATGGGTCACTTTCCGTAATAACAAAAACAACGAAGCCTTCCTCg < 1:2616770/68‑1 (MQ=255) tctcGCACACTCGAACGAGTCCGAATGGGTCACTTTCCGTAATAACAAAAACAACGAAGCCTTCCTCg < 1:3358334/68‑1 (MQ=255) tctcGCACACTCGAACGAGTCCGAATGGGTCACTTTCCGTAATAACAAAAACAACGAAGCCTTCCTCg < 1:208610/68‑1 (MQ=255) tctcGCACACTCGAACGAGTCCGAATGGGTCACTTTCCGTAATAACAAAAACAACGAAGCCTTCCTCg < 1:151118/68‑1 (MQ=255) cTCGAACGAGTCCGAATGGGTCACTTTCCGTAATAACAAAAACAACGAAGCCTTCCTCGATCGTGttt > 1:1012753/1‑68 (MQ=255) cTCGAACGAGTCCGAATGGGTCACTTTCCGTAATAACAAAAACAACGAAGCCTTCCTCGATCGTGTTTa > 1:3357219/1‑69 (MQ=255) cTCGAACGAGTCCGAATGGGTCACTTTCCGTAATAACAAAAACAACGAAGCCTTCCTCGATCGTGTTTa > 1:17789/1‑69 (MQ=255) cTCGAACGAGTCCGAATGGGTCACTTTCCGTAATAACAAAAACAACGAAGCCTTCCTCGATCGTGTTTa > 1:3222750/1‑69 (MQ=255) cTCGAACGAGTCCGAATGGGTCACTTTCCGTAATAACAAAAACAACGAAGCCTTCCTCGATCGTGTTTa > 1:3078213/1‑69 (MQ=255) cTCGAACGAGTCCGAATGGGTCACTTTCCGTAATAACAAAAACAACGAAGCCTTCCTCGATCGTGTTTa > 1:19315/1‑69 (MQ=255) cTCGAACGAGTCCGAATGGGTCACTTTCCGTAATAACAAAAACAACGAAGCCTTCCTCGATCGTGTTTa > 1:2968543/1‑69 (MQ=255) cTCGAACGAGTCCGAATGGGTCACTTTCCGTAATAACAAAAACAACGAAGCCTTCCTCGATCGTGTTTa > 1:2651605/1‑69 (MQ=255) cTCGAACGAGTCCGAATGGGTCACTTTCCGTAATAACAAAAACAACGAAGCCTTCCTCGATCGTGTTTa > 1:2318745/1‑69 (MQ=255) cTCGAACGAGTCCGAATGGGTCACTTTCCGTAATAACAAAAACAACGAAGCCTTCCTCGATCGTGTTTa > 1:23408/1‑69 (MQ=255) cGAACGAGTCCGAATGGGTCACTTTCCGTAATAACAAAAACAACGAAGCCTTCCTCGATCGTGTTTACa < 1:1621696/69‑1 (MQ=255) cGAACGAGTCCGAATGGGTCACTTTCCGTAATAACAAAAACAACGAAGCCTTCCTCGATCGTGTTTACa < 1:3128893/69‑1 (MQ=255) cGAACGAGTCCGAATGGGTCACTTTCCGTAATAACAAAAACAACGAAGCCTTCCTCGATCGTGTTTACa < 1:3093157/69‑1 (MQ=255) cGAACGACTCCGAATGGGTCACTTTCCGTAATAACAAAAACAACGAAGCCTTCCTCGATCGTGTTTACa < 1:677684/69‑1 (MQ=255) | TACAACGGGACGGAAGGTATCTCCGCCCTGCCGTTCAACGGGATTATTCTCGCACACTCGAACGAGTCCGAATGGGTCACTTTCCGTAATAACAAAAACAACGAAGCCTTCCTCGATCGTGTTTACA > minE/1184885‑1185011 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |