| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,186,871 | A→G | 24.2% | Q263Q (CAA→CAG) | yeaH → | conserved hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,186,871 | 0 | A | G | 24.2% | 142.1 / 40.8 | 115 | Q263Q (CAA→CAG) | yeaH | conserved hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base A (45/42); new base G (8/20); total (53/62) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.88e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.23e-01 | |||||||||||
Only 100 of 115 total aligned reads displayed.
CCGATCCCTCCAGCCAGGCAGTGATGTTTTGCCTGATGGACGTTTCCGGTTCAATGGATCAATCCACTAAAGATATGGCTAAGCGTTTTTATATTCTGCTGTATCTGTTCCTCAGCAGAA > minE/1186810‑1186929 | aTCCCTCCAGCCAGGCAGTGATG‑TTTGCCTGATGGACGTTTCCGGTTCAATGGATCAATCCACTAAAGa < 1:2011439/69‑1 (MQ=255) tCCCTCCAGCCAGGCAGTGATGTTTTGCCTGATGGACGTTTCCGGTTCAATGGATCAATCCACTAAAGa < 1:704243/69‑1 (MQ=255) tCCCTCCAGCCAGGCAGTGATGTTTTGCCTGATGGACGTTTCCGGTTCAATGGATCAATCCACTAAAGa < 1:3270374/69‑1 (MQ=255) tCCCTCCAGCCAGGCAGTGATGTTTTGCCTGATGGACGTTTCCGGTTCAATGGATCAATCCACTAAAGa < 1:3272623/69‑1 (MQ=255) tCCCTCCAGCCAGGCAGTGATGTTTTGCCTGATGGACGTTTCCGGTTCAATGGATCAATCCACTAAAGa < 1:3200551/69‑1 (MQ=255) tCCCTCCAGCCAGGCAGTGATGTTTTGCCTGATGGACGTTTCCGGTTCAATGGATCAATCCACTAAAGa < 1:1439268/69‑1 (MQ=255) tCCCTCCAGCCAGGCAGTGATGTTTTGCCTGATGGACGTTTCCGGTTCAATGGATCAATCCACTAAAGa < 1:2875813/69‑1 (MQ=255) tCCCTCCAGCCAGGCAGTGATGTTTTGCCTGATGGACGTTTCCGGTTCAATGGATCAATCCACTAAAGa < 1:616007/69‑1 (MQ=255) tCCCTCCAGCCAGGCAGTGATGTTTTGCCTGATGGACGTTTCCGGTTCAATGGATCAATCCACTAAAGa < 1:835989/69‑1 (MQ=255) tCCCTCCAGCCAGGCAGTGATGTTTTGCCTGATGGACGTTTCCGGTTCAATGGATCAATCCACTAAAGa < 1:1130728/69‑1 (MQ=255) tCCCTCCAGCCAGGCAGTGATGTTTTGCCTGATGGACGTTTCCGGTTCAATGGATCAATCCACTAAAGa < 1:205646/69‑1 (MQ=255) tCCCTCCAGCCAGGCAGTGATGTTTTGCCTGATGGACGTTTCCGGTTCAATGGATCAATCCACTAAAGa < 1:1038807/69‑1 (MQ=255) tCCCTCCAGCCAGGCAGTGATGTTTTGCCTGATGGACGTTTCCGGTTCAATGGATCAATCCACTAAAGa < 1:2058373/69‑1 (MQ=255) tCCCTCCAGCCAGGCAGTGATGTTTTGCCTGATGGACGTTTCCGGTTCAATGGATCAATCCACTAAAGa < 1:2069523/69‑1 (MQ=255) tCCCTCCAGCCAGGCAGTGATGTTTTGCCTGATGGACGTTTCCGGTTCAATGGATCAATCCACTAAAGa < 1:2097930/69‑1 (MQ=255) cccTCCAGCCAGGCAGTGATGTTTTGCCTGATGGACGTTTCCGGTTCAATGGATCAATCCACTTAAGa < 1:1711958/68‑1 (MQ=255) cccTCCAGCCAGGCAGTGATGTTTTGCCTGATGGACGTTTCCGGTTCAATGGATCAATCCACTAAAGat > 1:2523008/1‑69 (MQ=255) cccTCCAGCCAGGCAGTGATGTTTTGCCTGATGGACGTTTCCGGTTCAATGGATCAATCCACTAAAGat > 1:1213110/1‑69 (MQ=255) cccTCCAGCCAGGCAGTGATGTTTTGCCTGATGGACGTTTCCGGTTCAATGGATCAATCCACTAAAGat > 1:3371741/1‑69 (MQ=255) cccTCCAGCCAGGCAGTGATGTTTTGCCTGATGGACGTTTCCGGTTCAATGGATCAATCCACTAAAGa < 1:1512668/68‑1 (MQ=255) cccTCCAGCCAGGCAGTGATGTTTTGCCTGATGGACGTTTCCGGTTCAATGGATCAATCCACTAAAGa < 1:2107322/68‑1 (MQ=255) cccTCCAGCCAGGCAGTGATGTTTTGCCTGATGGACGTTTCCGGTTCAATGGATCAATCCACTAAAGa < 1:2659190/68‑1 (MQ=255) cccTCCAGCCAGGCAGTGATGTTTTGCCTGATGGACGTTTCCGGTTCAATGGATCAATCCACTAAAGa < 1:31722/68‑1 (MQ=255) cccTCCAGCCAGGCAGTGATGTTTTGCCTGATGGACGTTTCCGGTTCAATGGATCAATCCACTAAAGa < 1:2145143/68‑1 (MQ=255) cccTCCAGCCAGGCAGTGATGTTTTGCCTGATGGACGTTTCCGGTTCAATGGATCAATCCACTAAAGa < 1:2564888/68‑1 (MQ=255) cccTCCAGCCAGGCAGTGATGTTTTGCCTGATGGACGTTTCCGGTTCAATGGATCAATCCACTAAAGa < 1:1074939/68‑1 (MQ=255) cagccagGCAGTGATGTTTTGCCTGATGGACGTTTCCGGTTCAATGGATCAGTCCACTAAAGATATgg > 1:2892010/1‑68 (MQ=255) cagccagGCAGTGATGTTTTGCCTGATGGACGTTTCCGGTTCAATGGATCAGTCCACTAAAGATATgg > 1:2872774/1‑68 (MQ=255) cagccagGCAGTGATGTTTTGCCTGATGGACGTTTCCGGTTCAATGGATCAGTCCACTAAAGATATgg > 1:912647/1‑68 (MQ=255) cagccagGCAGTGATGTTTTGCCTGATGGACGTTTCCGGTTCAATGGATCAGTCCACTAAAGATATgg > 1:1022590/1‑68 (MQ=255) cagccagGCAGTGATGTTTTGCCTGATGGACGTTTCCGGTTCAATGGATCAGTCCACTAAAGATATgg > 1:1012097/1‑68 (MQ=255) cagccagGCAGTGATGTTTTGCCTGATGGACGTTTCCGGTTCAATGGATCAATCCACTAAAGATATgg > 1:3286007/1‑68 (MQ=255) cagccagGCAGTGATGTTTTGCCTGATGGACGTTTCCGGTTCAATGGATCAATCCACTAAAGATATgg > 1:2114174/1‑68 (MQ=255) cagccagGCAGTGATGTTTTGCCTGATGGACGTTTCCGGTTCAATGGATCAATCCACTAAAGATATgg > 1:620716/1‑68 (MQ=255) tGATGTTTTGCCTGATGGACGTTTCCGGTTCAATTGATCAGTCCACTAAAGATATGGCTAAACGttttt < 1:2652969/69‑1 (MQ=255) tGATGTTTTGCCTGATGGACGTTTCCGGTTCAATGGATCAGTCCACTAAAGATATGGCTAAACGttttt < 1:2434678/69‑1 (MQ=255) tGATGTTTTGCCTGATGGACGTTTCCGGTTCAATGGATCAGTCCACTAAAGATATGGCTAAACGttttt < 1:2605421/69‑1 (MQ=255) tGATGTTTTGCCTGATGGACGTTTCCGGTTCAATGGATCAGTCCACTAAAGATATGGCTAAACGttttt < 1:1006911/69‑1 (MQ=255) tGATGTTTTGCCTGATGGACGTTTCCGGTTCAATGGATCAGTCCACTAAAGATATGGCTAAACGttttt < 1:262730/69‑1 (MQ=255) tGATGTTTTGCCTGATGGACGTTTCCGGTTCAATGGATCAGTCCACTAAAGATATGGCTAAACGttttt < 1:31546/69‑1 (MQ=255) tGATGTTTTGCCTGATGGACGTTTCCGGTTCAATGGATCAGTCCACTAAAGATATGGCTAAACGttttt < 1:3367537/69‑1 (MQ=255) tGATGTTTTGCCTGATGGACGTTTCCGGTTCAATGGATCAGTCCACTAAAGATATGGCTAAACGttttt < 1:482580/69‑1 (MQ=255) tGATGTTTTGCCTGATGGACGTTTCCGGTTCAATGGATCAGTCCACTAAAGATATGGCTAAACGttttt < 1:554740/69‑1 (MQ=255) tGATGTTTTGCCTGATGGACGTTTCCGGTTCAATGGATCAGTCCACTAAAGATATGGCTAAACGttttt < 1:852122/69‑1 (MQ=255) tGATGTTTTGCCTGATGGACGTTTCCGGTTCAATGGATCAGTCCACTAAAGATATGGCTAAACGttttt < 1:928837/69‑1 (MQ=255) tGATGTTTTGCCTGATGGACGTTTCCGGTTCAATGGATCAGTCCACTAAAGATATGGCTAAACGttttt < 1:1159872/69‑1 (MQ=255) tGATGTTTTGCCTGATGGACGTTTCCGGTTCAATGGATCAGTCCACTAAAGATATGGCTAAACGttttt < 1:1439753/69‑1 (MQ=255) tGATGTTTTGCCTGATGGACGTTTCCGGTTCAATGGATCAGTCCACTAAAGATATGGCTAAACGttttt < 1:116394/69‑1 (MQ=255) tGATGTTTTGCCTGATGGACGTTTCCGGTTCAATGGATCAGTCCACTAAAGATATGGCTAAACGttttt < 1:118345/69‑1 (MQ=255) tGATGTTTTGCCTGATGGACGTTTCCGGTTCAATGGATCAGTCCACTAAAGATATGGCTAAACGttttt < 1:2161879/69‑1 (MQ=255) tGATGTTTTGCCTGATGGACGTTTCCGGTTCAATGGATCAGTCCACTAAAGATATGGCTAAACGttttt < 1:1783117/69‑1 (MQ=255) gATGTTTTGCCTGATGGACGTTTCCGGTTCAATGGATCAATCCACTAAAGa > 1:1006497/1‑51 (MQ=255) gATGTTTTGCCTGATGGACGTTTCCGGTTCAATGGATCAATCCACTAAAGa > 1:159564/1‑51 (MQ=255) gATGTTTTGCCTGATGGACGTTTCCGGTTCAATGGATCAATCCACTAAAGa > 1:1461261/1‑51 (MQ=255) gATGTTTTGCCTGATGGACGTTTCCGGTTCAATGGATCAATCCACTAAAGa > 1:3149041/1‑51 (MQ=255) gATGTTTTGCCTGATGGACGTTTCCGGTTCAATGGATCAATCCACTAAAGa > 1:3104282/1‑51 (MQ=255) gATGTTTTGCCTGATGGACGTTTCCGGTTCAATGGATCAATCCACTAAAGa > 1:3100237/1‑51 (MQ=255) gATGTTTTGCCTGATGGACGTTTCCGGTTCAATGGATCAATCCACTAAAGa > 1:2892973/1‑51 (MQ=255) gATGTTTTGCCTGATGGACGTTTCCGGTTCAATGGATCAATCCACTAAAGa > 1:53558/1‑51 (MQ=255) gATGTTTTGCCTGATGGACGTTTCCGGTTCAATGGATCAATCCACTAAAGa > 1:130434/1‑51 (MQ=255) gATGTTTTGCCTGATGGACGTTTCCGGTTCAATGGATCAATCCACTAAAGa > 1:2324937/1‑51 (MQ=255) gATGTTTTGCCTGATGGACGTTTCCGGTTCAATGGATCAATCCACAAAAg > 1:2483790/1‑50 (MQ=255) gATGGACGTTTCCGGTTCAATGGATCAATCCACTAAAGATATGGCTAAGCGTTTTTATATTCTGCTGTa > 1:2699304/1‑69 (MQ=255) gATGGACGTTTCCGGTTCAATGGATCAATCCACTAAAGATATGGCTAAGCGTTTTTATATTCTGCTGTa > 1:1804603/1‑69 (MQ=255) gATGGACGTTTCCGGTTCAATGGATCAATCCACTAAAGATATGGCTAAGCGTTTTTATATTCTGCTGTa > 1:430843/1‑69 (MQ=255) gATGGACGTTTCCGGTTCAATGGATCAATCCACTAAAGATATGGCTAAGCGTTTTTATATTCTGCTGTa > 1:1351736/1‑69 (MQ=255) gATGGACGTTTCCGGTTCAATGGATCAATCCACTAAAGATATGGCTAAGCGTTTTTATATTCTGCTGTa > 1:847625/1‑69 (MQ=255) gATGGACGTTTCCGGTTCAATGGATCAATCCACTAAAGATATGGCTAAGCGTTTTTATATTCTGCTGTa > 1:35052/1‑69 (MQ=255) gATGGACGTTTCCGGTTCAATGGATCAATCCACTAAAGATATGGCTAAGCGTTTTTATATTCTGCTGTa > 1:3411337/1‑69 (MQ=255) gATGGACGTTTCCGGTTCAATGGATCAATCCACTAAAGATATGGCTAAGCGTTTTTATATTCTGCTGTa > 1:2453624/1‑69 (MQ=255) gACGTTTCCGGTTCAATGGATCAATCCACTATATATATGGCTAAGCGTTTTTATATTCTGCTGTATCTg < 1:589540/69‑1 (MQ=255) gACGTTTCCGGTTCAATGGATCAATCCACTAAAGa < 1:534284/35‑1 (MQ=255) gACGTTTCCGGTTCAATGGATCAATCCACTAAAGa < 1:161624/35‑1 (MQ=255) gACGTTTCCGGTTCAATGGATCAATCCACTAAAGa < 1:2040270/35‑1 (MQ=255) gACGTTTCCGGTTCAATGGATCAATCCACTAAAGa < 1:2275380/35‑1 (MQ=255) gACGTTTCCGGTTCAATGGATCAATCCACTAAAGATATGGCTAAGCGTTTTTATATTCTGCTGTATCTg < 1:1596306/69‑1 (MQ=255) gACGTTTCCGGTTCAATGGATCAATCCACTAAAGATATGGCTAAGCGTTTTTATATTCTGCTGTATCTg < 1:766196/69‑1 (MQ=255) gACGTTTCCGGTTCAATGGATCAATCCACTAAAGATATGGCTAAGCGTTTTTATATTCTGCTGTATCTg < 1:1969145/69‑1 (MQ=255) gACGTTTCCGGTTCAATGGATCAATCCACTAAAGATATGGCTAAGCGTTTTTATATTCTGCTGTATCTg < 1:1394493/69‑1 (MQ=255) gACGTTTCCGGTTCAATGGATCAATCCACTAAAGATATGGCTAAGCGTTTTTATATTCTGCTGTATCTg < 1:855632/69‑1 (MQ=255) gACGTTTCCGGTTCAATGGATCAATCCACTAAAGATATGGCTAAGCGTTTTTATATTCTGCTGTATCTg < 1:928581/69‑1 (MQ=255) cGTTTCCGGTTCAATGGATCAATCCACTAAAGATATGGCTAAGCGTTTTTatat > 1:2514205/1‑54 (MQ=255) cGTTTCCGGTTCAATGGATCAATCCACTAAAGATATGGCTAAGCGTTTTTatat > 1:1951912/1‑54 (MQ=255) cGTTTCCGGTTCAATGGATCAATCCACTAAAGATATGGCTAAGCGTTTTTatat > 1:2454728/1‑54 (MQ=255) ttCAATGGATCAATCCACTAAAGATATGGCTAAGCGTTTTTATATTCTGCTGTATCTGTTCCTcagcag < 1:2888686/69‑1 (MQ=255) ttCAATGGATCAATCCACTAAAGATATGGCTAAGCGTTTTTATATTCTGCTGTATCTGTTCCTcagcag < 1:497725/69‑1 (MQ=255) ttCAATGGATCAATCCACTAAAGATATGGCTAAGCGTTTTTATATTCTGCTGTATCTGTTCCTcagcag < 1:1221570/69‑1 (MQ=255) cAATGGATCAATCCACTAAAGATATGGCTAAGCGTTTTTATATTCTGCTGTATCTGTTCCTCAGCAGaa > 1:2491711/1‑69 (MQ=255) cAATGGATCAATCCACTAAAGATATGGCTAAGCGTTTTTATATTCTGCTGTATCTGTTCCTCAGCAGaa > 1:82292/1‑69 (MQ=255) cAATGGATCAATCCACTAAAGATATGGCTAAGCGTTTTTATATTCTGCTGTATCTGTTCCTCAGCAGaa > 1:2395605/1‑69 (MQ=255) cAATGGATCAATCCACTAAAGATATGGCTAAGCGTTTTTATATTCTGCTGTATCTGTTCCTCAGCAGaa > 1:736313/1‑69 (MQ=255) cAATGGATCAATCCACTAAAGATATGGCTAAGCGTTTTTATATTCTGCTGTATCTGTTCCTCAGCAGaa > 1:1234879/1‑69 (MQ=255) cAATGGATCAATCCACTAAAGATATGGCTAAGCGTTTTTATATTCTGCTGTATCTGTTCCTCAGCAGaa > 1:2618770/1‑69 (MQ=255) cAATGGATCAATCCACTAAAGATATGGCTAAGCGTTTTTATATTCTGCTGTATCTGTTCCTCAGCAGaa > 1:1908032/1‑69 (MQ=255) cAATGGATCAATCCACTAAAGATATGGCTAAGCGTTTTTATATTCTGCTGTATCTGTTCCTCAGCAGaa > 1:1713586/1‑69 (MQ=255) cAATGGATCAATCCACTAAAGATATGGCTAAGCGTTTTTATATTCTGCTGTATCTGTTCCTCAGCAGaa > 1:1713401/1‑69 (MQ=255) cAATGGATCAATCCACTAAAGATATGGCTAAGCGTTTTTATATTCTGCTGTATCTGTTCCTCAGCAGaa > 1:3391040/1‑69 (MQ=255) cAATGGATCAATCCACTAAAGATATGGCTAAGCGTTTTTATATTCTGCTGTATCTGTTCCTCAGCAGaa > 1:3183811/1‑69 (MQ=255) cAATGGATCAATCCACTAAAGATATGGCTAAGCGTTTTTATATTCTGCTGTATCTGTTCCTCAGCAGaa > 1:1699406/1‑69 (MQ=255) cAATGGATCAATCCACTAAAGATATGGCTAAGCGTTTTTATATTCTGCTGTATCTGTTCCTCAGCAGaa > 1:239583/1‑69 (MQ=255) | CCGATCCCTCCAGCCAGGCAGTGATGTTTTGCCTGATGGACGTTTCCGGTTCAATGGATCAATCCACTAAAGATATGGCTAAGCGTTTTTATATTCTGCTGTATCTGTTCCTCAGCAGAA > minE/1186810‑1186929 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |