| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,190,401 | G→A | 61.0% | Q411Q (CAG→CAA) | yeaJ → | predicted diguanylate cyclase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,190,401 | 0 | G | A | 61.0% | 27.0 / 41.6 | 64 | Q411Q (CAG→CAA) | yeaJ | predicted diguanylate cyclase |
| Reads supporting (aligned to +/- strand): ref base G (18/7); new base A (15/24); total (33/31) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.12e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.88e-01 | |||||||||||
AAATGACACCCTCGGTCATCAGGAGGGGGATTTAGCGATTACGTTATTAGCTCAGGCGATTAAACAGTCGATTCGTAAAAGTGATTATGCCATCCGACTCGGTGGCGATGAATTCTGCATCATTCTTGTC > minE/1190335‑1190464 | aaatGACACCCTCGGTCATCAGGAGGGGGATTTAGCGATTACGTTATTAGCTCAGGCGATTAAACAGTc < 1:272281/69‑1 (MQ=255)aaatGACACCCTCGGTCATCAGGAGGGGGATTTAGCGATTACGTTATTAGCTCAGGCGATTAAACAGTc < 1:118807/69‑1 (MQ=255)aaatGACACCCTCGGTCATCAGGAGGGGGATTTAGCGATTACGTTATTAGCTCAGGCGATTAAACAGTc < 1:3002779/69‑1 (MQ=255)aaatGACACCATCGGTCATCAGGAGGGGGATTTAGCGATTACGTTATTAGCTCAGGCGATTAAACAGTc < 1:1449223/69‑1 (MQ=255) catcaGGAGGGGGATTTAGCGATTACGTTATTAGCTCAGGCGATTAAACAGTCGATTCGTAAAAGTGa > 1:2151070/1‑68 (MQ=255) catcaGGAGGGGGATTTAGCGATTACGTTATTAGCTCAGGCGATTAAACAGTCGATTCGTAAAAGTGAt > 1:1880800/1‑69 (MQ=255) catcaGGAGGGGGATTTAGCGATTACGTTATTAGCTCAGGCGATTAAACAGTCGATTCGTAAAAGTGAt > 1:2762757/1‑69 (MQ=255) catcaGGAGGGGGATTTAGCGATTACGTTATTAGCTCAGGCGATTAAACAGTCGATTCGTAAAAGTGAt < 1:1182617/69‑1 (MQ=255) catcaGGAGGGGGATTTAGCGATTACGTTATTAGCTCAGGCGATTAAACAGTCGATTCGTAAAAGTGAt < 1:2796204/69‑1 (MQ=255) catcaGGAGGGGGATTTAGCGATTACGTTATTAGCTCAGGCGATTAAACAGTCGATTCGTAAAAGTGAt > 1:3353291/1‑69 (MQ=255) gaggGGGATTTAGCGATTACGTTATTAGCTCAGGCGATTAAACAGTCGATTCGTAAAAGTGATTATGcc < 1:397427/69‑1 (MQ=255) ggggATTTAGCGATTACGTTATTAGCTCAGGCGATTAAACAGTCGATTCGTAAAAGTGATTATGCCAt > 1:2377079/1‑68 (MQ=255) ggATTTAGCGATTACGTTATTAGCTCAGGCGATTAAACAATc < 1:3273345/42‑1 (MQ=255) ggATTTAGCGATTACGTTATTAGCTCAGGCGATTAAACAATc < 1:252662/42‑1 (MQ=255) ggATTTAGCGATTACGTTATTAGCTCAGGCGATTAAACAATc < 1:2544066/42‑1 (MQ=255) ggATTTAGCGATTACGTTATTAGCTCAGGCGATTAAACAATc < 1:599388/42‑1 (MQ=255) ggATTTAGCGATTACGTTATTAGCTCAGGCGATTAAACAATc < 1:206130/42‑1 (MQ=255) ggATTTAGCGATTACGTTATTAGCTCAGGCGATTAAACAATc < 1:2052137/42‑1 (MQ=255) ggATTTAGCGATTACGTTATTAGCTCAGGCGATTAAACAATc < 1:2652899/42‑1 (MQ=255) ggATTTAGCGATTACGTTATTAGCTCAGGCGATTAAACAATc < 1:1962113/42‑1 (MQ=255) ggATTTAGCGATTACGTTATTAGCTCAGGCGATTAAACAATc < 1:1955846/42‑1 (MQ=255) ggATTTAGCGATTACGTTATTAGCTCAGGCGATTAAACAATc < 1:536006/42‑1 (MQ=255) ggATTTAGCGATTACGTTATTAGCTCAGGCGATTAAACAATc < 1:1668059/42‑1 (MQ=255) ggATTTAGCGATTACGTTATTAGCTCAGGCGATTAAACAATc < 1:2721611/42‑1 (MQ=255) ggATTTAGCGATTACGTTATTAGCTCAGGCGATTAAACAATc < 1:1145476/42‑1 (MQ=255) ggATTTAGCGATTACGTTATTAGCTCAGGCGATTAAACAATc < 1:3403988/42‑1 (MQ=255) ggATTTAGCGATTACGTTATTAGCTCAGGCGATTAAACAATc < 1:1194568/42‑1 (MQ=255) ggATTTAGCGATTACGTTATTAGCTCAGGCGATTAAACAATc < 1:1327498/42‑1 (MQ=255) ggATTTAGCGATTACGTTATTAGCTCAGGCGATTAAACAATc < 1:30348/42‑1 (MQ=255) ggATTTAGCGATTACGTTATTAGCTCAGGCGATTAAACAATc < 1:3169309/42‑1 (MQ=255) ttAGCGATTACGTTATTAGCTCAGGCGATTAAACAGTCGATTCGTAAAAGTGATTATGCCATCCGACt > 1:2280137/1‑68 (MQ=255) ttAGCGATTACGTTATTAGCTCAGGCGATTAAACAGTCGATTCGTAAAAGTGATTATGCCATCCGACTc > 1:2253851/1‑69 (MQ=255) ttAGCGATTACGTTATTAGCTCAGGCGATTAAACAGTCGATTCGTAAAAGTGATTATGCCATCCGACTc > 1:1068727/1‑69 (MQ=255) ttAGCGATTACGTTATTAGCTCAGGCGATTAAACAGTCGATTCGTAAAAGTGATTATGCCATCCGACTc > 1:1078944/1‑69 (MQ=255) ttAGCGATTACGTTATTAGCTCAGGCGATTAAACAGTCGATTCGTAAAAGTGATTATGCCATCCGACTc > 1:1121252/1‑69 (MQ=255) ttAGCGATTAAGTTATTAGCTCAGGCGATTAAACAGTCGAtt > 1:2931254/1‑42 (MQ=255) gATTAAACAATCGATTCGTAAAAGTGATTATGCCATCCGACTCGGTGGCGATGAATTCTGCATCATTc > 1:96189/1‑68 (MQ=255) gATTAAACAATCGATTCGTAAAAGTGATTATGCCATCCGACTCGGTGGCGATGAATTCTGCATCATTCt > 1:1580808/1‑69 (MQ=255) gATTAAACAATCGATTCGTAAAAGTGATTATGCCATCCGACTCGGTGGCGATGAATTCTGCATCATTCt > 1:2019275/1‑69 (MQ=255) gATTAAACAATCGATTCGTAAAAGTGATTATGCCATCCGACTCGGTGGCGATGAATTCTGCATCATTCt > 1:1157716/1‑69 (MQ=255) gATTAAACAATCGATTCGTAAAAGTGATTATGCCATCCGACTCGGTGGCGATGAATTCTGCATCATTCt > 1:3315473/1‑69 (MQ=255) gATTAAACAATCGATTCGTAAAAGTGATTATGCCATCCGACTCGGTGGCGATGAATTCTGCATCATTCt > 1:1373139/1‑69 (MQ=255) gATTAAACAATCGATTCGTAAAAGTGATTATGCCATCCGACTCGGTGGCGATGAATTCTGCATCATTCt > 1:897080/1‑69 (MQ=255) gATTAAACAATCGATTCGTAAAAGTGATTATGCCATCCGACTCGGTGGCGATGAATTCTGCATCATTCt > 1:683094/1‑69 (MQ=255) gATTAAACAATCGATTCGTAAAAGTGATTATGCCATCCGACTCGGTGGCGATGAATTCTGCATCATTCt > 1:2423494/1‑69 (MQ=255) gATTAAACAATCGATTCGTAAAAGTGATTATGCCATCCGACTCGGTGGCGATGAATTCTGCATCATTCt > 1:2928029/1‑69 (MQ=255) gATTAAACAATCGATTCGTAAAAGTGATTATGCCATCCGACTCGGTGGCGATGAATTCTGCATCATTCt > 1:2806113/1‑69 (MQ=255) gATTAAACAATCGATTCGTAAAAGTGATTATGCCATCCGACTCGGTGGCGATGAATTCTGCATCATTCt > 1:1552835/1‑69 (MQ=255) gATTAAACAATCGATTCGTAAAAGTGATTATGCCATCCGACTCGGTGGCGATGAATTCTGCATCATTCt > 1:888032/1‑69 (MQ=255) gATTAAACAATCGATTCGTAAAAGTGATTATGCCATCCGACTCGGTGGCGATGAATTCTGCATCATTCt > 1:2668677/1‑69 (MQ=255) gATTAAACAATCGATTCGTAAAAGTGACTATGCCATCCGACTCGGTGGCGATGAATTCTGCATCATTCt > 1:154661/1‑69 (MQ=255) ttAAACAATCGATTCGTAAAAGTGATTATGCCATCCGACTCGGTGGCGATGAATTCTGCATCATTCTTg < 1:1560398/69‑1 (MQ=255) ttAAACAATCGATTCGTAAAAGTGATTATGCCATCCGACTCGGTGGCGATGAATTCTGCATCATTCTTg < 1:272538/69‑1 (MQ=255) ttAAACAATCGATTCGTAAAAGTGATTATGCCATCCGACTCGGTGGCGATGAATTCTGCATCATTCTTg < 1:1296675/69‑1 (MQ=255) ttAAACAATCGATTCGTAAAAGTGATTATGCCATCCGACTCGGTGGCGATGAATTCTGCATCATTCTTg < 1:1579912/69‑1 (MQ=255) ttAAACAATCGATTCGTAAAAGTGATTATGCCATCCGACTCGGTGGCGATGAATTCTGCATCATTCTTg < 1:3420787/69‑1 (MQ=255) ttAAACAATCGATTCGTAAAAGTGATTATGCCATCCGACTCGGTGGCGATGAATTCTGCATCATTCTTg < 1:2241791/69‑1 (MQ=255) tAAACAGTCGATTCGTAAAAGTGATTATGCCATCCg > 1:1070329/1‑36 (MQ=255) tAAACAGTCGATTCGTAAAAGTGATTATGCCATCCGACTCGGTGGCGATGAATTCTGCATCATTCTTg > 1:3023858/1‑68 (MQ=255) tAAACAGTCGATTCGTAAAAGTGATTATGCCATCCGACTCGGTGGCGATGAATTCTGCATCATTCTTg > 1:1420589/1‑68 (MQ=255) tAAACAGTCGATTCGTAAAAGTGATTATGCCATCCGACTCGGTGGCGATGAATTCTGCATCATTCTTg > 1:963980/1‑68 (MQ=255) tAAACAGTCGATTCGTAAAAGTGATTATGCCATCCGACTCGGTGGCGATGAATTCTGCATCATTCTTg > 1:982916/1‑68 (MQ=255) aaaCAGTCGATTCGTAAAAGTGATTATGCCATCCGACTCGGTGGCGATGAATTCTGCATCATTCTTGt > 1:2757913/1‑68 (MQ=255) aaaCAGTCGATTCGTAAAAGTGATTATGCCATCCGACTCGGTGGCGATGAATTCTGCATCATTCTTGTc > 1:1450354/1‑69 (MQ=255) | AAATGACACCCTCGGTCATCAGGAGGGGGATTTAGCGATTACGTTATTAGCTCAGGCGATTAAACAGTCGATTCGTAAAAGTGATTATGCCATCCGACTCGGTGGCGATGAATTCTGCATCATTCTTGTC > minE/1190335‑1190464 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |