| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,195,448 | C→T | 30.3% | V568V (GTG→GTA) | yoaA ← | conserved hypothetical protein with nucleoside triphosphate hydrolase domain |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,195,448 | 0 | C | T | 30.3% | 88.4 / 42.1 | 92 | V568V (GTG→GTA) | yoaA | conserved hypothetical protein with nucleoside triphosphate hydrolase domain |
| Reads supporting (aligned to +/- strand): ref base C (31/33); new base T (13/15); total (44/48) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.63e-01 | |||||||||||
CGTCGGCGTCGCGAATCAGTCGCCCTACCCCCTGTTTGAGAGTAATGACGGCATCTGGTAGTTGCACTTCATCGAACGGGTCGCCACCGCGCAAACGACAATCTTCCATGCGCGCTTTTAACAGTGGATCA > minE/1195384‑1195514 | cgtcgGCGTCGCGAATCAGTCGCCCTACCCCCTGTTTGAGAGTAATGACGGCATCTGGTAGTTGCACtt < 1:1028066/69‑1 (MQ=255)cgtcgGCGTCGCGAATCAGTCGCCCTACCCCCTGTTTGAGAGTAATGACGGCATCTGGTAGTTGCACtt < 1:1079845/69‑1 (MQ=255)cgtcgGCGTCGCGAATCAGTCGCCCTACCCCCTGTTTGAGAGTAATGACGGCATCTGGTAGTTGCACtt < 1:2419128/69‑1 (MQ=255) gcgAATCAGTCGCCCTACCCCCTGTTTGAGAGTAATGACGGCATCTGGTAGTTGCACTTCa > 1:1156197/1‑61 (MQ=255) gcgAATCAGTCGCCCTACCCCCTGTTTGAGAGTAATGACGGCATCTGGTAGTTGCACTTCCTCGCACgg > 1:2063533/1‑69 (MQ=255) gcgAATCAGTCGCCCTACCCCCTGTTTGAGAGTAATGACGGCATCTGGTAGTTGCACTTCATCGAACgg > 1:971574/1‑69 (MQ=255) gcgAATCAGTCGCCCTACCCCCTGTTTGAGAGTAATGACGGCATCTGGTAGTTGCACTTCATCGAACgg > 1:3149768/1‑69 (MQ=255) gcgAATCAGTCGCCCTACCCCCTGTTTGAGAGTAATGACGGCATCTGGTAGTTGCACTTCATCGAACgg > 1:2945335/1‑69 (MQ=255) gcgAATCAGTCGCCCTACCCCCTGTTTGAGAGTAATGACGGCATCTGGTAGTTGCACTTCATCGAACgg > 1:2330523/1‑69 (MQ=255) gcgAATCAGTCGCCCTACCCCCTGTTTGAGAGTAATGACGGCATCTGGTAGTTGCACTTCATCGAACgg > 1:167126/1‑69 (MQ=255) gAATCAATCGCCCTACCCCCTGCTTGAGAGTAATGACCGCATCCGGCAGTTGTACTTCATCGAACgga < 1:2063211/68‑2 (MQ=255) gAATCAATCGCCCTACCCCCTGCTTGAGAGTAATGACCGCATCCGGCAGTTGTACTTCATCGAACgga < 1:2442992/68‑2 (MQ=255) gAATCAATCGCCCTACCCCCTGCTTGAGAGTAATGACCGCATCCGGCAGTTGTACTTCATCGAACgga < 1:1954520/68‑2 (MQ=255) gAATCAATCGCCCTACCCCCTGCTTGAGAGTAATGACCGCATCCGGCAGTTGTACTTCATCGAACgga < 1:2511617/68‑2 (MQ=255) gAATCAATCGCCCTACCCCCTGCTTGAGAGTAATGACCGCATCCGGCAGTTGTACTTCATCGAACgga < 1:2719335/68‑2 (MQ=255) gAATCAATCGCCCTACCCCCTGCTTGAGAGTAATGACCGCATCCGGCAGTTGTACTTCATCGAACgga < 1:273599/68‑2 (MQ=255) gAATCAATCGCCCTACCCCCTGCTTGAGAGTAATGACCGCATCCGGCAGTTGTACTTCATCGAACgga < 1:3177260/68‑2 (MQ=255) gAATCAATCGCCCTACCCCCTGCTTGAGAGTAATGACCGCATCCGGCAGTTGTACTTCATCGAACgga < 1:1081096/68‑2 (MQ=255) gAATCAATCGCCCTACCCCCTGCTTGAGAGTAATGACCGCATCCGGCAGTTGTACTTCATCGAACgga < 1:1279117/68‑2 (MQ=255) gAATCAATCGCCCTACCCCCTGCTTGAGAGTAATGACCGCATCCGGCAGTTGTACTTCATCGAACgga < 1:1521142/68‑2 (MQ=255) gAATCAATCGCCCTACCCCCTGCTTGAGAGTAATGACCGCATCCGGCAGTTGTACTTCATCGAACgga < 1:1559398/68‑2 (MQ=255) gAATCAATCGCCCTACCCCCTGCTTGAGAGTAATGACCGCATCCGGCAGTTGTACTTCATCGAACgga < 1:2795261/68‑2 (MQ=255) gAATCAATCGCCCTACCCCCTGCTTGAGAGTAATGACCGCATCCGGCAGTTGTACTTCATCGAACgga < 1:2927747/68‑2 (MQ=255) aatcaatcGCCCTACCCCCTGCTTGAGAGTAATGACCGCATCCGGCAGTTGTACTTCATCGAACgga < 1:1550896/67‑2 (MQ=255) cttGAGAGTAATGACCGCATCCGGCAGTTGTACTTCATCGAACgga < 1:2088320/45‑2 (MQ=25) tGAGAGTAATGACGGCATCTGGTAGTTGCACTTCATCGAACGGGTCGCCACCGCGCAAACGACAATCt > 1:1572431/1‑68 (MQ=255) tGAGAGTAATGACGGCATCTGGTAGTTGCACTTCATCGAACGGGTCGCCACCGCGCAAACGACAATCt > 1:1109759/1‑68 (MQ=255) tGAGAGTAATGACGGCATCTGGTAGTTGCACTTCATCGAACGGGTCGCCACCGCGCAAACGACAATCt > 1:2753097/1‑68 (MQ=255) tGAGAGTAATGACGGCATCTGGTAGTTGCACTTCATCGAACGGGTCGCCACCGCGCAAACGACAATCt > 1:3281235/1‑68 (MQ=255) tGAGAGTAATGACGGCATCTGGTAGTTGCACTTCATCGAACGGGTCGCCACCGCGCAAACGACAATCt > 1:1388854/1‑68 (MQ=255) tGAGAGTAATGACGGCATCTGGTAGTTGCACTTCATCGAACGGGTCGCCACCGCGCAAACGACAATCt > 1:2606907/1‑68 (MQ=255) tGAGAGTAATGACGGCATCTGGTAGTTGCACTTCATCGAACGGGTCGCCACCGCGCAAACGACAATCt > 1:3343862/1‑68 (MQ=255) tGAGAGTAATGACGGCATCTGGTAGTTGCACTTCATCGAACGGGTCGCCACCGCGCAAACGACAATCt > 1:369364/1‑68 (MQ=255) tGAGAGTAATGACGGCATCTGGTAGTTGCACTTCATCGAACGGGTCGCCACCGCGCAAACGACAATCt > 1:792803/1‑68 (MQ=255) tGAGAGTAATGACGGCATCTGGTAGTTGCACTTCATCGAACGGGTCGCCACCGCGCAAACGACAATCt > 1:2046670/1‑68 (MQ=255) tGAGAGTAATGACGGCATCTGGTAGTTGCACTTCATCGAACGGGTCGCCACCGCGCAAACGACAATCt > 1:2279381/1‑68 (MQ=255) tGAGAGTAATGACGGCATCTGGTAGTTGCACTTCATCGAACGGGTCGCCACCGCGCAAACGACAATCt > 1:1309215/1‑68 (MQ=255) agagTAATGACGGCATCTGGTAGTTGCACTTCATCGAACGGTTCGCCACCGCGCAAACGACAATCTTc < 1:1027920/68‑1 (MQ=255) agagTAATGACGGCATCTGGTAGTTGCACTTCATCGAACGGGTCGCCACCGCGCAAACGACAATCTTc < 1:3168337/68‑1 (MQ=255) agagTAATGACGGCATCTGGTAGTTGCACTTCATCGAACGGGTCGCCACCGCGCAAACGACAATCTTc < 1:1497078/68‑1 (MQ=255) agagTAATGACGGCATCTGGTAGTTGCACTTCATCGAACGGGTCGCCACCGCGCAAACGACAATCTTc < 1:3299977/68‑1 (MQ=255) agagTAATGACGGCATCTGGTAGTTGCACTTCATCGAACGGGTCGCCACCGCGCAAACGACAATCTTc < 1:1205203/68‑1 (MQ=255) agagTAATGACGGCATCTGGTAGTTGCACTTCATCGAACGGGTCGCCACCGCGCAAACGACAATCTTc < 1:1737149/68‑1 (MQ=255) gACGGCATCTGGTAGTTGCACTTCATCTAACGGGTCGCCACCGCGCAAACGACAATCTTCCATgcgcg < 1:1240699/68‑1 (MQ=255) gACGGCATCTGGTAGTTGCACTTCATCGAACGGGTCGCCACCGCGCAAACGACAATCTTCCATgcgcg < 1:592706/68‑1 (MQ=255) gACGGCATCTGGTAGTTGCACTTCATCGAACGGGGCGCCACCGCGCAAACGACAATCTTc < 1:1649760/60‑1 (MQ=255) gCATCTGGTAGTTGCACTTCATCGAACGGGTCGCCACCGCGCAAACGACAATCTTCCATGCGCGCtttt < 1:2651409/69‑1 (MQ=255) gCATCCGGCAGTTGTACTTCATCGAACGGATCACCACCGCGCAAACGGCAATCTTcc > 1:2750770/1‑57 (MQ=255) gCATCCGGCAGTTGTACTTCATCGAACGGATCACCACCGCGCAAACGGCAATCTTCCATGCGTGCtttt > 1:1766677/1‑69 (MQ=255) gCATCCGGCAGTTGTACTTCATCGAACGGATCACCACCGCGCAAACGGCAATCTTCCATGCGTGCtttt > 1:3059343/1‑69 (MQ=255) gCATCCGGCAGTTGTACTTCATCGAACGGATCACCACCGCGCAAACGGCAATCTTCCATGCGTGCtttt > 1:2940174/1‑69 (MQ=255) gCATCCGGCAGTTGTACTTCATCGAACGGATCACCACCGCGCAAACGGCAATCTTCCATGCGTGCtttt > 1:1385147/1‑69 (MQ=255) gCATCCGGCAGTTGTACTTCATCGAACGGATCACCACCGCGCAAACGGCAATCTTCCATGCGTGCtttt > 1:3382154/1‑69 (MQ=255) gCATCCGGCAGTTGTACTTCATCGAACGGATCACCACCGCGCAAACGGCAATCTTCCATGCGTGCtttt > 1:369199/1‑69 (MQ=255) gCATCCGGCAGTTGTACTTCATCGAACGGATCACCACCGCGCAAACGGCAATCTTCCATGCGTGCtttt > 1:1372538/1‑69 (MQ=255) gCATCCGGCAGTTGTACTTCATCGAACGGATCACCACCGCGCAAACGGCAATCTTCCATGCGTGCtttt > 1:2619957/1‑69 (MQ=255) gCATCCGGCAGTTGTACTTCATCGAACGGATCACCACCGCGCAAACGGCAATCTTCCATGCGTGCtttt > 1:371337/1‑69 (MQ=255) gCATCCGGCAGTTGTACTTCATCGAACGGATCACCACCGCGCAAACGGCAATCTTCCATGCGTGCtttt > 1:420987/1‑69 (MQ=255) gCATCCGGCAGTTGTACTTCATCGAACGGATCACCACCGCGCAAACGGCAATCTTCCATGCGTGCtttt > 1:765249/1‑69 (MQ=255) gCATCCGGCAGTTGTACTTCATCGAACGGATCACCACCGCGCAAACGGCAATCTTCCATGCGTGCtttt > 1:646772/1‑69 (MQ=255) tCTGGTAGTTGCACTTCATCGAACGGGTCGCCACCGCGCAAACGACAATCTTCCATGCGCGCTTTTAAc < 1:3023428/69‑1 (MQ=255) tGGTAGTTGCACTTCATCGAACGGGTCGCCACCGCGCAAACGACaa > 1:297784/1‑46 (MQ=255) tGGTAGTTGCACTTCATCGAACGGGTCGCCACCGCGCAAACGACAATCTTc < 1:3388037/51‑1 (MQ=255) tGGTAGTTGCACTTCATCGAACGGGTCGCCACCGCGCAAACGACAATCTTc < 1:221187/51‑1 (MQ=255) tGGTAGTTGCACTTCATCGAACGGGTCGCCACCGCGCAAACGACAATCTTc < 1:2266175/51‑1 (MQ=255) tGGTAGTTGCACTTCATCGAACGGGTCGCCACCGCGCAAACGACAATCTTc < 1:2777270/51‑1 (MQ=255) tGGTAGTTGCACTTCATCGAACGGGTCGCCACCGCGCAAACGACAATCTTCCATGCGCGCTTTTAACAg > 1:1580254/1‑69 (MQ=255) tGGTAGTTGCACTTCATCGAACGGGTCGCCACCGCGCAAACGACAATCTTCCATGCGCGCTTTTAACAg > 1:1848657/1‑69 (MQ=255) tGGTAGTTGCACTTCATCGAACGGGTCGCCACCGCGCAAACGACAATCTTCCATGCGCGCTTTTAACAg > 1:150283/1‑69 (MQ=255) tGCACTTCATCGAACGGGTCGCCACCGCGCAAACGACAATCTTCCATGCGCGCTTTTAACAg < 1:426174/62‑1 (MQ=255) tGCACTTCATCGAACGGGTCGCCACCGCGCAAACGACAATCTTCCATGCGCGCTTTTAACAg < 1:330389/62‑1 (MQ=255) tGCACTTCATCGAACGGGTCGCCACCGCGCAAACGACAATCTTCCATGCGCGCTTTTAACAg < 1:1321120/62‑1 (MQ=255) tGCACTTCATCGAACGGGTCGCCACCGCGCAAACGACAATCTTCCATGCGCGCTTTTAACAg < 1:1516221/62‑1 (MQ=255) tGCACTTCATCGAACGGGTCGCCACCGCGCAAACGACAATCTTCCATGCGCGCTTTTAACAg < 1:1289505/62‑1 (MQ=255) tGCACTTCATCGAACGGGTCGCCACCGCGCAAACGACAATCTTCCATGCGCGCTTTTAACAg < 1:3010453/62‑1 (MQ=255) tGCACTTCATCGAACGGGTCGCCACCGCGCAAACGACAATCTTCCATGCGCGCTTTTAACAg < 1:2938959/62‑1 (MQ=255) tGCACTTCATCGAACGGGTCGCCACCGCGCAAACGACAATCTTCCATGCGCGCTTTTAACAg < 1:2898657/62‑1 (MQ=255) tGCACTTCATCGAACGGGTCGCCACCGCGCAAACGACAATCTTCCATGCGCGCTTTTAACAg < 1:1795558/62‑1 (MQ=255) tGCACTTCATCGAACGGGTCGCCACCGCGCAAACGACAATCTTCCATGCGCGCTTTTAACAg < 1:2716879/62‑1 (MQ=255) tGCACTTCATCGAACGGGTCGCCACCGCGCAAACGACAATCTTCCATGCGCGCTTTTAACAg < 1:2667696/62‑1 (MQ=255) tGCACTTCATCGAACGGGTCGCCACCGCGCAAACGACAATCTTCCATGCGCGCTTTTAACAg < 1:3148908/62‑1 (MQ=255) tGCACTTCATCGAACGGGTCGCCACCGCGCAAACGACAATCTTCCATGCGCGCTTTTAACAg < 1:258581/62‑1 (MQ=255) tGCACTTCATCGAACGGGTCGCCACCGCGCAAACGACAATCTTCCATGCGCGCTTTTAACAg < 1:2017928/62‑1 (MQ=255) tGCACTTCATCGAACGGGTCGCCACCGCGCAAACGACAATCTTCCATGCGCGCTTTTAACAg < 1:2300593/62‑1 (MQ=255) tGCACTTCATCGAACGGGTCGCCACCGCGCAAACGACAATCTTCCATGCGCGCTTTTAACAGTGGatca > 1:1840823/1‑69 (MQ=255) tGCACTTCATCGAACGGGTCGCCACCGCGCAAACGACAATCTTCCATGCGCGCTTTTAACAGTGGatca > 1:2156353/1‑69 (MQ=255) tGCACTTCATCGAACGGGTCGCCACCGCGCAAACGACAATCTTCCATGCGCGCTTTTAACAGTGGatca > 1:2058449/1‑69 (MQ=255) tGCACTTCATCGAACGGGTCGCCACCGCGCAAACGACAATCTTCCATGCGCGCTTTTAACAGTGGatca > 1:2441812/1‑69 (MQ=255) tGCACTTCATCGAACGGGTCGCCACCGCGCAAACGACAATCTTCCATGCGCGCTTTTAACAGTGGatca > 1:429907/1‑69 (MQ=255) tGCACTTCATCGAACGGGTCGCCACCGCGCAAACGACAATCTTCCATGCGCGCTTTTAACAGTGGatca > 1:295028/1‑69 (MQ=255) tGCACTTCATCGAACGGGTCGCCACCGCGCAAAAGACAATCTTCCATGCGCGCTTTTAACAGTGGatca > 1:1423318/1‑69 (MQ=255) gCACTTCATCGAACGGGTCGCCACCGCGCAAACGACAATCTTCCATgc > 1:1195563/1‑48 (MQ=255) | CGTCGGCGTCGCGAATCAGTCGCCCTACCCCCTGTTTGAGAGTAATGACGGCATCTGGTAGTTGCACTTCATCGAACGGGTCGCCACCGCGCAAACGACAATCTTCCATGCGCGCTTTTAACAGTGGATCA > minE/1195384‑1195514 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |